PUBLICATIONS 1. K.V. NGUYEN and J.C. GALIN Anti-inflammatory polymer-bound steroids for topical applications I: Synthesis and characterization. Journal of Applied Polymer Science, 30, 2761-2778 (1985). 2. K.V. NGUYEN and L. JUNG Study of the retention of child-dose drugs on cellulose ester membranes during in-line intravenous filtration. Sciences Techniques et Pratiques Pharmaceutiques, 1, 201-207 (1985). 3. K.V. NGUYEN, L. JUNG, G. COUPIN and P. POINDRON Anti-inflammatory polymer-bound steroids for topical applications II: Controlled release of the steroids. Journal of Polymer Science: Polymer Chemistry Edition, 24, 359-373 (1986). 4. B. RIHN, F. BISSERET, R. GIRARDOT, J.M. SCHEFTEL, K.V. NGUYEN and H. MONTEIL Fast protein purification of Clostridium difficile cytotoxin. Journal of Chromatography, 428, 408-414 (1988). 5. K.V. NGUYEN, C. HECKEL, F. BISSERET, B. RIBIN, R. GIRARDOT and H. MONTEIL Enzyme-linked immunosorbent assay for detection of Clostridium difficile toxine B. Archives of Toxicology, Suppl. 12, 453-456 (1988). 6. F. BISSERET, G. KEITH, B. RIHN, I. AMIRI, B. WERNEBURG, R. GIRARDOT, O. BALDACINI, G. GREEN, K.V. NGUYEN and H. MONTEIL Clostridium difficile toxin B: Characterization and sequence of three peptides. Journal of Chromatography, 490, 91-100 (1989). Curriculum Vitae Khue Nguyen 14 7. K.V. NGUYEN, B. RIHN, C. HECKEL, F. BISSERET, R. GIRARDOT and H. MONTEIL Enzyme immunoassay (ELISA) for detection of Clostridium difficile toxin B in specimens of faeces. Journal of Medical Microbiology, 31, 251-257 (1990). 8. K.V. NGUYEN, C.M. WOLFF, J.L. SERIS and J.P. SCHWING Study on a complete lyophilizate for rapid colorimetric enzymatic creatinine assay. Analusis, 18, 582-587 (1990). 9. K.V. NGUYEN, C.M. WOLFF, J.L. SERIS and J.P. SCHWING Immobilized enzyme electrode for creatinine determination in serum. Analytical Chemistry, 63, 611-614 (1991). 10. K.V. NGUYEN and R.F. MAES Evaluation of an enzyme-linked immunosorbent assay for detection of antibodies to bovine leukemia virus in serum and milk. Journal of Clinical Microbiology, 31, 979-981 (1993). 11. K.V. NGUYEN, L.E. SABATIER, M. GOELDNER, C. BOUDIER, G. JAMET, J.M. WARTER and P. POINDRON Stabilization of dry immobilized acetylcholinesterase on microtitration plates for colorimetric determination of its inhibitors in water and biological fluids. Enzyme and Microbial Technology, 20, 18-23 (1997). 12. K.V. NGUYEN, C.M. WOLFF, J.M. WARTER and P. POINDRON Stabilization of dry immobilized acetylcholinesterase on nitrocellulose membrane for rapid colorimetric screening of its inhibitors in water and biological fluids. Analytical Letters, 31, 2457-2473 (1998). Curriculum Vitae Khue Nguyen 15 13. K.V. NGUYEN, N. LECLERC, C.M. WOLFF, P. KENNEL, P. FONTENEAU, R. DEYES, J.M. WARTER and P. POINDRON Protection of immunoreactivity of dry immobilized proteins on microtitration plates in ELISA: Application for detection of autoantibodies in Myasthenia gravis. Journal of Biotechnology, 72, 115-125 (1999). 14. K.V. NGUYEN, C.M. WOLFF, D. MEYER, P. POINDRON and J.M. WARTER Utilization of nucleotide probes for the measurement of specific messenger RNA: Application for molecular diagnosis of autosomal recessive spinal muscular atrophy. Analytical Letters, 35, 1135-1148 (2002). 15. K.V. NGUYEN, J.L. GENDRAULT and C.M. WOLFF Poly-L-lysine dissolves fibrillar aggregation of the Alzheimer ?-amyloid peptide in vitro. Biochemical and Biophysical Research Communications, 291, 764-768 (2002). 16. C.M. WOLFF, K.V. NGUYEN and P. REMY Cloning and expression of the Surfeit locus member Surf-6 during embryogenesis in Xenopus laevis. DNA Sequence, 13, 149-152 (2002). The nucleotide sequence reported in this paper has been deposited in the GenBank data base under the accession number AJ276843.1. 17. R.K. NAVIAUX and K.V. NGUYEN POLG mutations associated with Alpers syndrome and mitochondrial DNA depletion. Annals of Neurology, 55, 706-712 (2004). 18. K.V. NGUYEN RT-PCR-based cloning of the human beta-amyloid precursor protein gene and the construction of its expression plasmids. Analytical Letters, 38, 71-88 (2005). Curriculum Vitae Khue Nguyen 16 19. K.V. NGUYEN RT-PCR-based cloning of human SMN, the SMA determining gene, and the construction of its expression plasmids. Analytical Letters, 38, 1351-1365 (2005). 20. R.K. NAVIAUX and K.V. NGUYEN POLG mutations associated with Alpers syndrome and mitochodrial DNA depletion. Annals of Neurology, 58, 491 (2005). 21. K.V. NGUYEN, E. OSTERGAARD, S.H. RAVN, T. BALSLEV, E.R. DANIELSEN, A. VARDAG, P.J. McKIERNAN, G. GRAY and R.K. NAVIAUX POLG mutations in Alpers syndrome. Neurology, 65, 1493-1495 (2005). 22. K.V. NGUYEN Selection of peptide ligands specific for baculovirus DNA-binding protein from the FliTrxTM random peptide display library. Analytical Letters, 39, 99-112 (2006). 23. K.V. NGUYEN, F. SHARIEF, S. S. L. CHAN, W.C. COPELAND and R.K. NAVIAUX Molecular diagnosis of Alpers syndrome. Journal of Hepatology, 45, 108-116 (2006). 24. K.V. NGUYEN Utilization of nucleotide probes in ELISA procedure for the quantitative determination of baculovirus titer. Analytical Letters, 40, 513-528 (2007). 25. K.V. NGUYEN Utilization of nucleotide probes in PCR-ELISA procedure for the quantitative determination of Plasmodium falciparum DNA in malaria. Analytical Letters, 40, 2045- 2065 (2007). 26. K.V. NGUYEN Human p53 and Hdm2: Cloning and construction of expression plasmid. Analytical Letters, 43, 323-334 (2010). Curriculum Vitae Khue Nguyen 17 27. K.V. NGUYEN, R.K. NAVIAUX, S. PATRA, B.A. BARSHOP and W.L. NYHAN Novel mutations in the human MCCA and MCCB gene causing methylcrotonylglycinuria. Molecular Genetics and Metabolism, 102, 218-221 (2011). 28. K.V. NGUYEN, R.K. NAVIAUX, K.K. PAIK and W.L. NYHAN Novel mutations in the human HPRT gene. Nucleosides, Nucleotides and Nucleic Acids, 30, 440-445 (2011). 29. K.V. NGUYEN, R.K. NAVIAUX, K.K. PAIK and W.L. NYHAN Lesch-Nyhan syndrome: mRNA expression of HPRT in patients with enzyme proven deficiency of HPRT and normal HPRT coding region of the DNA. Molecular Genetics and Metabolism, 106, 498-501 (2012). 30. K.V. NGUYEN, R.K. NAVIAUX, K.K. PAIK, T. NAKAYAMA and W.L. NYHAN Lesch-Nyhan variant syndrome: real-time RT-PCR for mRNA quantification in variable presentation in three affected family members. Nucleosides, Nucleotides and Nucleic Acids, 31, 616-629 (2012). 31. K.V. NGUYEN, R. NGUYEN and W.L. NYHAN The human folate receptor 1 gene: molecular diagnostic of folate deficiency. International Journal of Medicine and Molecular Medicine, 3(6):WMC003461 (2012). 32. K.V. NGUYEN Antithrombin Hanoi: Arg 393 to His missense point mutation in antithrombin gene and cancer. International Journal of Medicine and Molecular Medicine, 3(9):WMC003720 (2012). 33. K.V. NGUYEN and W.L. NYHAN Identification of novel mutations in the human HPRT gene. Nucleosides, Nucleotides and Nucleic Acids, 32, 155-160 (2013). Curriculum Vitae Khue Nguyen 18 34. R. FU, I.C. PICOT, R.J. TORRES, L.E. LAROVERE, Y. YAMADA, K.V. NGUYEN, M. HEGDE, J.E. VISSER, D.J. SCHRETLEN, W.L. NYHAN, J.G. PUIG, P.J. O’NEILL, H.A. JINNAH and for the Lesch-Nyhan Disease International Group. Genotype-phenotype correlations in neurogenetics: Lesch-Nyhan disease as a model disorder. Brain, 137, 1282-1303 (2014). 35. K.V. NGUYEN Epigenetic regulation in amyloid precursor protein and the Lesch-Nyhan syndrome. Biochemical and Biophysical Research Communications, 446, 1091-1095 (2014). 36. K.V. NGUYEN The human ?-amyloid precursor protein: biomolecular and epigenetic aspects. Biomolecular Concepts, 6, 11-32 (2015). 37. K.V. NGUYEN and W.L. NYHAN Lesch-Nyhan syndrome in a family with a deletion followed by an insertion within the HPRT1 gene. Nucleosides, Nucleotides and Nucleic Acids, 34, 442-447 (2015). 38. K.V. NGUYEN Epigenetic regulation in amyloid precursor protein with genomic rearrangements and the Lesch-Nyhan syndrome. Nucleosides, Nucleotides and Nucleic Acids, 34, 674-690 (2015). 39. K.V. NGUYEN and W.L. NYHAN Mutation in the human HPRT1 gene and the Lesch-Nyhan syndrome. Nucleosides, Nucleotides and Nucleic Acids, 35, 426-433 (2016). Curriculum Vitae Khue Nguyen 19 40. K.V. NGUYEN, R.K. NAVIAUX and W.L. NYHAN Human HPRT1 gene and the Lesch-Nyhan disease: substitution of alanine for glycine and inversely in the HGprt enzyme protein. Nucleosides, Nucleotides and Nucleic Acids, 36, 151-157 (2017). 41. K.V. NGUYEN and W.L. NYHAN Quantification of various APP-mRNA isoforms and epistasis in Lesch-Nyhan disease. Neuroscience Letters, 643, 52-58 (2017). 42. K.V. NGUYEN, K. LEYDIKER, R. WANG, J. ABDENUR and W.L. NYHAN A neurodevelopmental disorder with a nonsense mutation in the Ox-2 antigen domain of the amyloid precursor protein (APP) gene. Nucleosides, Nucleotides and Nucleic Acids, 36, 317-327 (2017). 43. K.V. NGUYEN, S. SILVA, M. TRONCOSO, R.K. NAVIAUX and W.L. NYHAN Lesch-Nyhan disease in two families from Chiloe Island with mutations in the HPRT1 gene. Nucleosides, Nucleotides and Nucleic Acids, 36, 452-462 (2017). 44. K.V. NGUYEN Epigenetics in rare diseases. Journal of Rare Disorders: Diagnosis & Therapy, 3:1 (2017). 45. K.V. NGUYEN, R.K. NAVIAUX and W.L. NYHAN Novel mutation in the human HPRT1 gene and the Lesch-Nyhan disease. Nucleosides, Nucleotides and Nucleic Acids (submitted). 46. K.V. NGUYEN Epigenetics in rare diseases and the target for epigenetic therapy. Biomolecular Concepts

Dr. Calixto Machado graduated as MD in 1976. He continued his medical training to become Specialist in Neurology and Clinical Neurophysiology, First Degree in 1980, and Second Degree in 1984. In 1994 he became PhD, and in 2002 he was the youngest Dr. in Sciences (PhD - Second Degree) in his country. Dr. Machado received clinical training in specialized neurology centers in Sweden, Denmark, Canada, Italy, Austria, and the US. During the last 30 years he has run many research protocols, such as: brain death, coma, persistent vegetative states and other disorders of consciousness, stroke, autism, etc. He has published more than 450 peer reviewed articles, book chapters, and 7 books. His Book “Brain Death: A Reappraisal” has been recognized with great enthusiasm among neurologist, neurosurgeons, intensivists, and physicians specialized in transplants.