Hurdle Concept and The Role of Lactic Acid Bacteria

Review Article | DOI: https://doi.org/10.31579/2637-8914/248

Hurdle Concept and The Role of Lactic Acid Bacteria

  • Zakari David Adeiza
  • Kareem Sarafadeen Olateju
  • Bello Kizito Eneye
  • Audu Godwin Amoka
  • Aliyu Abdulbasit Anoze
  • Muhammed Abdulsamad Adeiza
  • Osazuwa Christopher Olojemarin
  • Emurotu Marvelous Olubunmi

1Department of Microbiology, Kogi State University (Prince Abubakar Audu University), PMB 1008, Anyigba, Kogi State, Nigeria

2Department of Microbiology, College of Biosciences, Federal University of Agriculture, PMB 2240, Abeokuta, Ogun State, Nigeria 

3Department of Medical Microbiology and Parasitology, School of Medical Sciences, Universiti Sains, Malaysia

4Department of Chemistry, Prince Abubakar Audu University, PMB1008, Anyigba, Kogi State, Nigeria

5Department of Microbiology, Adekunle Ajasin University, Akungba, Ondo State, Nigeria

*Corresponding Author: Zakari David Adeiza, 1Department of Microbiology, Kogi State University (Prince Abubakar Audu University), PMB 1008, Anyigba, Kogi State, Nigeria.

Citation: Zakari D. Adeiza, Kareem S. Olateju, Bello K. Eneye, Audu G. Amoka, Aliyu A. Anoze, et al, (2024), Hurdle Concept and The Role of Lactic Acid Bacteria, J. Nutrition and Food Processing, 7(10); DOI:10.31579/2637-8914/248

Copyright: © 2024, Zakari David Adeiza. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Received: 08 July 2024 | Accepted: 13 August 2024 | Published: 23 August 2024

Keywords: lactic acid bacteria; diacetyl; biofilms; acetic acid; food matrices

Abstract

The hurdle idea is a food preservation technique that includes building up a number of barriers to prevent microbes from growing and prolong the shelf life of perishable goods. The idea of using lactic acid bacteria (LAB), which are essential for food preservation, is one that is significant. Through a variety of processes, including fermentation, competitive exclusion, metabolite generation, biofilm formation, nutritional competition, and metabolic activity, LAB support the hurdle concept. Lactic acid bacteria (LAB) ferment glucose to produce lactic acid, which results in an acidic environment that prevents infections and spoiling germs from growing. Additionally, LAB generates antimicrobial substances such as hydrogen peroxide, organic acids, and bacteriocins, which work by competitively excluding harmful germs from growing. Furthermore, metabolites of lactic acid bacteria (LAB) such acetic acid, diacetyl, and carbon dioxide enhance the flavor of fermented foods and serve as organic preservatives. Moreover, LAB have the ability to create biofilms that shield them from antimicrobial agents and environmental stressors, increasing their survival and persistence in food matrices. By means of nutritional competition, LAB outcompetes pathogenic or spoilage bacteria in the consumption of available resources, hence restricting their growth. In addition, LAB produces enzymes and antibacterial substances, among other metabolic activities that further impede the growth of infections. 

Introduction

The hurdle idea is a method of food preservation that combines the use of several non-lethal barriers to stop pathogenic and spoilage bacteria from growing and surviving in food. Physical, chemical, and biological variables are some of the obstacles. Because it minimizes the use of preservatives and other potentially dangerous chemicals while preserving the nutritional value, flavor, and texture of food, this method of food preservation has gained popularity in recent years (Panel, 2022).

The hurdle concept and the function of lactic acid bacteria (LAB) are important factors in food preservation since they guarantee the durability and safety of perishable food items. The hurdle concept is using several barriers or hurdles to prevent microbes from growing and prolong food shelf life. Important players in this notion are lactic acid bacteria, a varied group of bacteria recognized for their capacity to create lactic acid through fermentation. 

Fundamentally, the hurdle concept seeks to establish a setting in which microbes find it difficult to endure and multiply. The total microbial load in food is reduced by combining several barriers, such as temperature regulation, pH adjustment, water activity reduction, and preservatives, increasing the stability and safety of food products. Because of their special qualities and contributions to preventing the proliferation of pathogens and spoilage bacteria, LAB play a major role in this framework (Nura et al., 2016).

LAB, which include a variety of species like Lactobacillus, Lactococcus, Leuconostoc, and Streptococcus, are ubiquitous in nature. These microorganisms are frequently present in fermented foods and give them their unique tastes, textures, and scents. Food products have a lower pH because LAB convert carbohydrates into lactic acid through metabolic processes. This environment's acidity hinders the development of spoiling microorganisms as well as the spread of harmful bacteria (Malheiros et al., 2015).

Furthermore, the many metabolites and chemicals that LAB create further impede the growth of unwanted microbes. For example, they create natural preservatives such as hydrogen peroxide, organic acids, and bacteriocins, which are antibacterial substances. These substances show inhibitory actions against pathogens and spoilage bacteria, adding another line of defense against microbial contamination (Nura et al., 2016).

LAB also support the hurdle notion by outcompeting other microbes for resources and available space through a process known as competitive exclusion. Because of their better nutrient utilization skills, LAB are able to inhibit the growth of harmful or spoilage bacteria and efficiently absorb available resources. LAB can also create biofilms, which increase their survival and persistence in food matrices by providing defense against environmental stressors and antimicrobial agents (Kaya et al., 2019).

Food manufacturers are able to increase the safety, sensory qualities, and shelf life of perishable foods by utilizing LAB and the hurdle concept. The application of LAB in a range of culinary items, including cheese, yogurt, sauerkraut, kimchi, and sourdough bread, demonstrates their adaptability and significance in the maintenance and evolution of unique flavors in these goods (Leal-Sánchez et al., 2002).

History and Evolution:

Van Valen initially presented the idea of hurdle technology in the early 1960s. He suggested using a number of low-level physical and chemical barriers to stop microorganisms from growing in food. Subsequently, the hurdle theory has been improved and broadened to encompass more biological and physical variables. Over time, the hurdle notion has grown and changed to encompass a wider variety of physical, chemical, and biological elements that are involved in food preservation. Van Valen first offered the idea in the early 1960s, suggesting the use of several low-level chemical and physical barriers to stop microorganisms from growing in food. Since then, other components and their combinations have been added to the hurdle concept, which has been improved and broadened (Panel, 2022).

Scientific Principles:

The hurdle concept is founded on the scientific idea that a variety of low-level stressors working together can limit or completely stop microorganisms in food from growing and surviving. These stress variables, which collectively prevent the development and survival of bacteria, include temperature, pH, water activity, preservatives, and antimicrobial compounds. Because of their combined effect, less preservative and antibacterial agent is needed than would be the case if these components were used independently (Parada et al., 2007).

Practical Applications:

A wide range of food products, including meats, dairy products, fruits, and vegetables, have been effectively preserved using the hurdle idea. The hurdle idea is frequently used in the meat industry to produce cooked and cured meats, which require the use of heat, nitrite or nitrate, low pH, and low water activity. Similar to this, lactic acid bacteria and low pH are utilized in the dairy industry to create fermented foods like kefir, yogurt, and cheese using the hurdle idea. The idea of the hurdle is also applied to the preservation of fruits and vegetables, wherein low pH, heat, and refrigeration are combined to keep the produce fresh and unspoiled (Panel, 2022).

In order to stop the growth of microorganisms and lengthen the shelf life of food, the hurdle idea in food preservation refers to a technique that combines several barriers or elements. The steps involved in applying the hurdle principle to food preservation are as follows:

Steps involved in the use of hurdle concept in food preservation

1. Hurdle Selection: The first stage is to determine which hurdles will be utilized to keep the food fresh. A number of variables, including temperature, pH, water activity, chemical preservatives, packaging, and altered environment, might operate as hurdles (Malheiros et al., 2015).

2. Analysis of Microbial Targets: It's critical to know which particular microbes are most likely to proliferate in the food that is being preserved. The right barriers can be chosen with the aid of this analysis (Kaya et al., 2019).

3. Determining obstacle Parameters: Each obstacle requires a set of parameters that are determined based on the study. For example, the type of food and the microorganisms present can be used to establish the target temperature, pH level, water activity, or concentration of preservatives (Mahrous et al., 2013).

4. Combination of Hurdles: Combining several hurdles is a crucial component of the hurdle concept. Various obstacles combine to prevent microbial development. Finding the ideal combination to optimize the preservation effect is crucial (Kaya et al., 2019).

5. Application of Barriers: After defining the barriers and their limitations, the meal is subjected to them. This may entail procedures such as heat treatment (such as pasteurization or sterilization), pH level adjustments, preservative additions, packing under modified atmospheres, or high-pressure processing (Oladapo et al., 2014).

6. Monitoring and Control: To make sure that the barriers are successful in stopping microbial development, the preservation process must be watched over. The effectiveness of the hurdle system can be confirmed through routine testing, such as microbiological analysis can be performed to verify the efficacy of the hurdle system (Nura et al., 2016).

7. Storage and Distribution: Following the preservation procedure, the food can be kept and shared in suitable settings, keeping in mind the particular obstacles that were employed. To keep the barriers functional, handling, packing, and storage temperatures must all be followed (Leistner, 2000).

8. Determining Shelf Life: Over time, quality and safety factors are assessed to ascertain how long a preserved product will last. This entails assessing any deterioration or spoiling through chemical analysis, microbiological testing, and sensory evaluation (Panel, 2022).

Factors used in food preservation

Temperature: A key element in the preservation of food is temperature. Food microbes are frequently destroyed or rendered inactive by heat treatment, and food microbiological quality is greatly dependent on temperature control. In order to attain the required degree of preservation, hurdle technology employs combinations of heat treatments at various temperatures (Oladapo et al., 2014).

Acidity/pH: Another significant variable that can be controlled for food preservation is acidity or pH. Because some microbes arepH sensitive, reducing a food product's pH can prevent them from growing and surviving. The pH levels of food products have been adjusted by the use of organic acids as well as other acids including citric, lactic, and acetic acids (Nura et al., 2016).

Water Activity: A food product's water molecule availability is gauged by its water activity (aw), with a lower aw signifying a smaller amount of available water molecules. Because certain microbes require high aw levels to thrive, microbial development and spoiling can be prevented by decreasing aw through drying, salting, or the use of other chemicals (Oladapo et al.,2014).

Preservatives: To stop microbes from growing, preservatives are frequently employed in food preservation. With hurdle technology, the same amount of microbial inhibition that would normally need larger doses of single preservatives is achieved by using many preservatives, frequently in low quantities (Leal-Sánchez et al., 2002). 

Antimicrobial Agents: Materials that can stop the growth of bacteria include synthetic and natural antimicrobial agents like bacteriocins. The food business makes extensive use of bacteriocins, which are proteins made by specific bacteria that either kill or stop the growth of other bacteria (Kaya et al., 2019).

Biological Processes: Dairy products, meats, and vegetables are frequently preserved using biological processes like fermentation and the use of starter cultures. During fermentation, carbohydrates are broken down by microbes, most often lactic acid bacteria, which result in the production of organic acids and other substances that aid in the regulation of microbial development and enhance the quality of food (Malheiros et al., 2015). 

Various food products that employs hurdle technology

1. Cooked and Cured Meats: Haze technique is frequently used to preserve foods including sausages, deli meats, and cooked ham. This calls for the application of several techniques, including low pH (from acids or fermentation), low water activity (from drying or salt addition), heat treatment (cooking or smoking), and the use of nitrite or nitrate as preservatives (Nura et al., 2016).

2. Fermented Dairy Products: Using hurdle technology, fermented dairy products include kefir, cheese, and yogurt. A mixture of lactic acid bacteria ferment lactose to produce lactic acid, which in turn creates an acidic environment that prevents the growth of pathogenic microbes and spoiling (Kaya et al., 2019).

3. Fruit and Vegetable Preservation: To retain quality and increase shelf life, fresh fruits and vegetables can be preserved using hurdle technology. To reduce microbial and enzymatic activity, methods like refrigeration, modified atmosphere packaging (MAP), and temperature and humidity management are used (Panel, 2022).

4. Pickles and Sauerkraut: The hurdle principle is used to preserve pickled vegetables, such as cucumbers and sauerkraut. The flavors and textures are enhanced and rotting is prevented by the combination of high salt concentrations, low pH (from fermentation or vinegar), and chilling (Oladapo et al., 2014).

5. Shelf-Stable Ready-to-Eat Meals: The manufacturing of shelf-stable, ready-to-eat meals frequently uses hurdle technology. To preserve safety and increase shelf life, a variety of preservation procedures are used, including low pH, low water activity, vacuum packaging, thermal processing (canning), and oxygen removal methods (Nura et al., 2016). 

6. Bakery Products: Hinderneath technology can also be used to preserve baked items such as bread, cakes, and pastries. Preservatives, temperature control, low water activity (from sugar or reduced water content), and altered packaging methods are some of the strategies used to keep product quality and stop microbiological deterioration (Malheiros et al., 2015).

How are the factors of food preservation employed in hurdle concept

In order to stop microbiological development and lengthen the shelf life of food, the hurdle concept in food preservation combines a number of hurdles, such as temperature, pH, water activity, moisture content, chemical preservatives, packaging, and changed environment. Each of these obstacles is used as follows:

1. Temperature: One of the biggest obstacles to food preservation is temperature. Microorganisms can be killed by high temperatures or their growth might be slowed down by low temperatures (Leistner, 2000). The type of food, the microorganisms present, and the preservation method all play a role in determining the temperature. For instance, pasteurization entails heating the food to a precise temperature (often between 72°C and 75°C) in order to kill the majority of the bacteria, whereas sterilization entails heating the food to a higher temperature (sometimes beyond 100°C) in order to eradicate all germs (Kaya et al., 2019).

2. pH: Another significant barrier to food preservation is pH. Many bacteria are inhibited in their growth by acidity. Food can be made more acidic and less conducive to microbial development by adjusting its pH level using organic acids such as citric acid, lactic acid, or acetic acid (Walter, 2008).

3. Water activity: The quantity of water that is available for microbial growth in a food product is known as water activity (aw). Since microorganisms require water to develop, lowering the water activity may prevent them from doing so. Drying, adding sugar or salt, or utilizing other dehydrating agents can all help achieve this (Yi et al., 2013).

4. Moisture content: Moisture content is a significant barrier to food preservation and is correlated with water activity. It is possible to lower the food's moisture content by drying, dehydration, or desiccation processes, which prevent microbial growth (Zúñiga et al., 1993).

5. Chemical preservatives: To stop or slow down the growth of germs in food, chemicals such organic acids, salt, nitrites, and sulfites are frequently utilized as barriers. The kind of microorganisms present and the food product being preserved determine the chemical preservative's type and concentration (Ulloa et al., 2008).

6. Packaging: Food preservation is greatly aided by packaging. By establishing a barrier between the food and the environment, it can stop microbiological growth. Using materials for airtight packaging will help achieve this. Additionally, some packaging methods can lower the oxygen content and produce an anaerobic environment that prevents the growth of bacteria (Ulloa et al., 2008).

7. Modified atmosphere: It is possible to alter the environment around food in order to prevent the growth of microorganisms. This can be accomplished by changing the air's composition in the packaging material to include a mix of nitrogen, oxygen, and carbon dioxide. Packaging with a modified environment can be helpful for fresh items like meat products and fruits and vegetables (Van Geel-Schutten et al., 1998).

In order to stop microbes from growing, food preservation experts use a combination of barriers, such as temperature, pH, water activity, moisture level, chemical preservatives, packaging, and a modified atmosphere. The application of each of these barriers depends on the particular food product, the kind of microorganisms present, and the preservation method being employed (Ulloa et al., 2008).

How does hurdle concept help in extending the shelf life of perishable foods

1. Microbial Growth Inhibition: The hurdle idea establishes an environment that prevents the growth of microbes by using a variety of hurdles, including temperature, pH, water activity, moisture content, chemical preservatives, packing, and changed atmosphere. This prolongs the shelf life by preventing deterioration and the growth of foodborne germs (Walter, 2008). 

2. Combination Effect: The barriers in the hurdle idea cooperate with one another to increase their efficacy against microbes. When combined, for instance, low temperature, packaging in a modified environment, and chemical preservatives can significantly suppress microbial development more than when any one of these barriers is used alone (Yi et al., 2013).
3. Reduction of Water Activity: Since microorganisms need water to thrive, the barrier idea produces an environment that is adverse to microbial growth by reducing the water activity by methods including drying, dehydration, or the addition of salt or sugar. This slows down the rate of spoiling, extending the shelf life of perishable items (Zúñiga et al., 1993). 

4. Preservation of Quality Attributes: The hurdle idea aids in maintaining the quality characteristics of perishable foods in addition to preventing microbial development (Kaban and Kaya, 2008). For example, keeping the food at the right temperature, utilizing packaging with a modified environment, and managing moisture content can all help to efficiently preserve the meal's sensory qualities by reducing oxidative reactions, delaying physical deterioration, and slowing down enzymatic activity (Jang et al., 2006).

5. Safety Assurance: By preventing the formation of foodborne germs, the hurdle concept not only increases the shelf life of perishable goods but also guarantees their safety. By controlling harmful germs, a safer product is provided for consumers through the use of proper chemical preservatives and pH modification, among other challenges (Kaban and Kaya, 2008).

6. Shelf Life Prediction and Extension: The hurdle idea makes it possible to better understand the shelf life of perishable foods through regular monitoring, shelf life prediction models, and quality assessments (Kadariya et al., 2014). The items' shelf life can be increased by modifying and optimizing the hurdle characteristics, which will decrease food waste and boost profitability.

Perishable goods can be kept safe from infection and deterioration by using the hurdle idea in food preservation, and ensuring an extended shelf life while maintaining their quality and safety (Kaya et al., 2019).

Chemical preservatives commonly used in hurdle concept

1. Benzoates: Common preservatives include potassium and sodium benzoate. They work well against some bacteria, molds, and yeasts. Pickles, fruit juices, salad dressings, fizzy drinks, and pickles all frequently include benzoates (KP et al., 2016).

2. Sorbates: Common preservatives include calcium and potassium sorbate. They work well against some bacteria, yeasts, and molds. Sorbates are frequently found in wine, cheese, baked products, and dried fruits (Leal-Sánchez et al., 2002).

3. Sulfites: Strong preservatives with antibacterial and antioxidant qualities, sulfites include sulfur dioxide, sodium sulfite, and potassium metabisulfite. They are frequently found in wine, dried fruits, canned vegetables, and seafood and are efficient against a variety of bacteria (Leistner, 2000).

4. Nitrites and Nitrates: To stop the growth of Clostridium botulinum and other dangerous bacteria, processed meat products like bacon, ham, and sausages frequently contain potassium and sodium nitrate. Additionally, nitrates and nitrites contribute to the distinct flavor, color, and shelf life of cured meats (Mahrous et al., 2013).

5. Organic Acids: Preservatives such as lactic acid, malic acid, acetic acid, and citric acid work well. They produce an acidic environment that prevents microbial growth by lowering the pH of the meal. Organic acids are frequently found in drinks, dressings, sauces, and dairy products (Malheiros et al., 2015).

6. Propionates: To prevent the formation of mold and germs, calcium and sodium propionate are frequently added to bread and other baked foods. They aid in extending the shelf life of baked goods and are efficient against rotting microbes (Mandal et al., 2008). 

7. Parabens: Two common food preservatives found in baked goods, confections, and beverages are methyl and propylparaben. They work well against germs, molds, and yeasts (McDonald et al., 1987).

8. EDTA, or ethylenediaminetetraacetic acid, is a chelating chemical that helps maintain the quality and freshness of some foods, particularly those that are processed and canned. It binds to metal ions that can catalyze unwanted reactions, preventing the deterioration of texture, flavor, and color (Mead et al., 1999).

Role of Lactic Acid Bacteria in the preservation of fermented dairy products through hurdle technology

They contribute to the safety, flavor, and shelf life of these products by performing the following functions in preservation process:

1. Acidification: Lactose, the naturally occurring sugar in milk, is fermented by LAB to produce lactic acid (Mokoena et al., 2016). The dairy product's pH is lowered via acidification, which also produces an acidic environment that prevents the growth of harmful bacteria and spoiling. The product's flavor and texture are also preserved by the higher acidity (Naidu et al., 1999).

2. Competitive Exclusion: Because LAB can grow in the low pH environment they produce, they have an edge over many other microbes (Nura et al., 2016). LAB quickly acidify the product, making it uninhabitable for more potentially hazardous bacteria, yeasts, and molds. By limiting the proliferation of spoilage germs, this competitive exclusion reduces the likelihood of foodborne diseases (Oladapo et al., 2014).

3. Production of Preservative Compounds: During fermentation, LAB create a variety of organic compounds, including lactic acid, hydrogen peroxide, and bacteriocins. By preventing the growth of harmful microbes and deterioration, these substances function as organic preservatives (Onwuakor et al., 2014). LAB specifically produces proteinaceous substances called bacteriocins, which have antibacterial effect against bacteria that are closely related to one another or compete with it (Oppegård et al., 2010).

4. Development of Flavor and Aroma: LAB gives fermented dairy products their distinct flavors and fragrances. Lactic acid and other chemicals such as acetic acid, diacetyl, and volatiles are produced during fermentation by lactose breakdown by lactic acid bacteria (Panel, 2022). These substances improve the product's sensory qualities by imparting to it the characteristically acidic, sour, or creamy flavors of fermented dairy products (Parada et al., 2007).

5. Shelf Life Extension: Fermented dairy products have a longer shelf life due to the interaction of acidity, competition, and antibacterial chemicals produced by LAB (Perez et al., 2014). The risk of spoiling and waste is decreased because the controlled growth of LAB prevents the growth of bacteria that cause spoiling and helps maintain product quality for a longer amount of time (Rajilić-Stojanović and De Vos, 2014).

How different strains of lactic acid bacteria impact the flavor and texture of fermented dairy products

1. Streptococcus thermophilus: Yogurt is typically made using this common culture of bacteria. It produces lactic acid by fermenting lactose, which lowers pH and coagulates milk proteins to give yogurt its sour flavor and creamy texture (Saxena et al., 2009). Exopolysaccharides, which are also produced by S. thermophilus, enhance the end product's viscosity and texture (Sidhu and Nehra, 2019).

2. Lactobacillus bulgaricus: This common yogurt culture is also essential in giving yogurt its characteristically sour taste (Hati et al., 2013). L. bulgaricus coagulates milk proteins and helps give yogurt its creamy texture, working in tandem with S. thermophilus. But L. bulgaricus is also capable of producing volatile substances like diacetyl and acetaldehyde, which give yogurt its distinct flavor (Herbel et al., 2013).

3. Lactobacillus delbrueckii subsp. bulgaricus: Because of the particular bacterial culture utilized, this strain of bacteria is utilized to produce Bulgarian yogurt, which differs slightly from regular yogurt (Alkema et al., 2016). Bulgarian yogurt's smooth, creamy texture is produced by the fermentation of lactose into lactic acid by Lactobacillus delbrueckii subsp. bulgaricus, which also lowers pH. It also yields a unique flavor that is sometimes characterized as being somewhat acidic and tasting like fresh cheese (Aditya and Nida, 2015).

4. Lactococcus lactis: This bacteria is used to make a variety of cheeses, such as feta, Gouda, and cheddar. Cheese gets its distinctive pores and texture from the fermentation of lactose into lactic acid and carbon dioxide. The creation of the cheese flavor is also aided by the bacteria's breakdown of the milk proteins (Alakomi et al., 2000).

5. Bifidobacteria: Some yogurts and other fermented dairy products are made with the help of bifidobacteria. They have a reputation for having possible health benefits, including as boosting immunity and enhancing digestive health (Aljewicz and Cichosz, 2017). These goods might have a smooth, creamy texture and a mild, somewhat sweet flavor thanks to the impact of bifidobacteria (Alkema et al., 2016).

These are just a few examples, but the specific bacterial strains used, as well as the fermentation conditions and processing techniques, can significantly impact the final product characteristics (Anurag Chaturvedi et al., 2013). The choice of bacterial culture used in the fermentation process can greatly influence the sensory attributes, texture, and nutritional value of fermented dairy products (Arora et al., 2009).

Some specific food products that employs lactic acid bacteria in its hurdle concept

Lactic acid bacteria (LAB) can be employed in several specific food products using the hurdle concept to inhibit the growth of spoilage microorganisms and pathogens (Bazhal et al., 2003). Here are some examples:

1) Cheese: The fermentation process in the making of cheese is started with LAB. Lactic acid is produced by LAB during this process, which acidifies the cheese curd and makes it uninhabitable for pathogens and bacteria that cause spoiling (Bourdichon et al., 2019). The main application of some LAB strains, such as Lactobacillus helveticus and Lactococcus lactis, is in the making of cheese, which gives different types of cheese their distinct flavor, texture, and scent (De Vuyst and Leroy, 2007).

2) Yogurt: Lactic acid bacteria (LAB) are essential for the fermentation of lactose, a milk sugar, into lactic acid, which lowers pH and imparts a tangy flavor to yogurt (Devi et al., 2013). Lactobacillus bulgaricus and Streptococcus thermophilus are two particular LAB strains that are frequently employed in the manufacturing of yogurt; during fermentation, they also create vital vitamins and nutrients (Diep et al., 2009).

3) Sauerkraut: LAB use lactic acid fermentation to turn cabbage into sauerkraut, which has a sour, tart flavor that is typical of fermented foods (Dimov et al., 2005). The LAB Lactobacillus plantarum and Leuconostoc mesenteroides are in charge of fermenting sauerkraut. Because lactic acid production lowers the pH of sauerkraut, spoilage bacteria cannot thrive there, extending the product's shelf life (Djadouni and Kihal, 2012).

4) Kimchi: A fermented vegetable food that goes through lactic acid fermentation, kimchi is similar to sauerkraut (Elayaraja et al., 2014). Leuconostoc mesenteroides, Lactobacillus kimchii, and Lactobacillus plantarum are LAB species that aid in the fermentation of kimchi. In addition to extending the product's shelf life, LAB give kimchi its distinct flavor and color (Field et al., 2018).

5) Sourdough bread: To produce sourdough bread, LAB are used in the fermentation process, which gives the dough a sour taste. Lactobacillus sanfranciscensis and Lactobacillus brevis are the main LAB strains used in sourdough fermentation; they enhance the bread's shelf life and aid in the formation of taste and fragrance molecules (Ganguly, 2013).

Challenges and solutions to hurdle concept 

Combining a number of barriers or hurdles to stop the growth of microbes and increase the shelf life of food items is known as the hurdle concept in food preservation. Although it provides practical preservation solutions, there are certain implementation-related difficulties. 

The following are some typical obstacles and possible ways to overcome them in relation to the hurdle concept:

1. Microbial Adaptation: The efficiency of individual barriers can be decreased when microorganisms learn to adapt and become resistant to one or more obstacles found in the food environment. Reduced shelf life and impaired safety may result from this (Goh and Philip, 2015).

Solution: Evaluate and modify the set of obstacles utilized in the food preservation procedure on a regular basis. The effectiveness of barriers can be maintained and microbial adaption can be inhibited by rotating between different preservation procedures (Goldstein et al., 2015).

2. Synergistic Effects: When several obstacles are employed together, they may have a synergistic effect that produces a preservation outcome that is greater than when they are utilized alone. It can be difficult to figure out the best combination and dosage of obstacles, though (Gordon, 2016).

Solution: To determine the best combination and concentration of obstacles for a given food product, conduct extensive research and trial. Extensive testing and collaboration with food scientists can yield important insights on optimization and synergy (Güllüce et al., 2013).

3. Quality and Sensory Changes: During the preservation process, some obstacles like intense heat or potent preservatives might change the food products' sensory characteristics and quality. Consumer acceptability may be impacted by this (Gunathilake, 2005).

Solution: Look into different barriers or altered processing methods that can minimize detrimental effects on sensory attributes while still achieving microbial control. For instance, replacing chemical preservatives with natural antimicrobials or applying a gentler heat treatment (Hati et al., 2013).

4. Cost and Implementation: The barrier concept may need to be implemented with additional testing, equipment, and monitoring, which could be costly and logistically difficult, particularly for smaller food enterprises (Herbel et al., 2013).

Solution: Make the best use of the tools and resources already in place while looking at more affordable options for implementing the barrier. Partnering with academic institutions or business associations can facilitate access to resources, shared knowledge, and funding. 
5. Regulatory Compliance: Regulations and rules pertaining to the installation of hurdle technology, including the concentrations of permissible hurdles, may vary based on the geographical region (Hu et al., 2010).

Recommendation

Maintain awareness of the particular rules and policies in the intended market. Consult a professional, interact with regulatory bodies, and make sure local food safety laws are being followed. 

Conclusion

The hurdle idea is a flexible and successful method of food preservation that combines a number of non-lethal elements to stop microbes from growing and surviving in food. Improved food safety, longer shelf life, and a decrease in the need of preservatives and other potentially dangerous chemicals are just a few advantages of using this strategy. Several food products, including as meats, dairy products, fruits, and vegetables, have been produced effectively using the hurdle idea. The obstacle idea is probably going to become a more crucial tactic in the food business as consumer desire for naturally occurring, minimally processed goods grows. The hurdle notion has evolved over time to encompass a variety of physical, chemical, and biological elements that combine to produce successful food preservation techniques. Combining these variables enables the use of preservative and antibacterial agent dosage reductions with the same degree of microbial inhibition. The barrier concept will keep changing as new methods of preservation, as well as new components and procedures, are created to assist satisfy the increasing consumer desire for naturally occurring, minimally processed foods.

References

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Journal of Neuroscience and Neurological Surgery. I had the experience of publishing a research article recently. The whole process was simple from submission to publication. The reviewers made specific and valuable recommendations and corrections that improved the quality of my publication. I strongly recommend this Journal.

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Orlando Villarreal

Dr. Katarzyna Byczkowska My testimonial covering: "The peer review process is quick and effective. The support from the editorial office is very professional and friendly. Quality of the Clinical Cardiology and Cardiovascular Interventions is scientific and publishes ground-breaking research on cardiology that is useful for other professionals in the field.

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Katarzyna Byczkowska

Thank you most sincerely, with regard to the support you have given in relation to the reviewing process and the processing of my article entitled "Large Cell Neuroendocrine Carcinoma of The Prostate Gland: A Review and Update" for publication in your esteemed Journal, Journal of Cancer Research and Cellular Therapeutics". The editorial team has been very supportive.

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Anthony Kodzo-Grey Venyo

Testimony of Journal of Clinical Otorhinolaryngology: work with your Reviews has been a educational and constructive experience. The editorial office were very helpful and supportive. It was a pleasure to contribute to your Journal.

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Pedro Marques Gomes

Dr. Bernard Terkimbi Utoo, I am happy to publish my scientific work in Journal of Women Health Care and Issues (JWHCI). The manuscript submission was seamless and peer review process was top notch. I was amazed that 4 reviewers worked on the manuscript which made it a highly technical, standard and excellent quality paper. I appreciate the format and consideration for the APC as well as the speed of publication. It is my pleasure to continue with this scientific relationship with the esteem JWHCI.

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Bernard Terkimbi Utoo

This is an acknowledgment for peer reviewers, editorial board of Journal of Clinical Research and Reports. They show a lot of consideration for us as publishers for our research article “Evaluation of the different factors associated with side effects of COVID-19 vaccination on medical students, Mutah university, Al-Karak, Jordan”, in a very professional and easy way. This journal is one of outstanding medical journal.

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Prof Sherif W Mansour

Dear Hao Jiang, to Journal of Nutrition and Food Processing We greatly appreciate the efficient, professional and rapid processing of our paper by your team. If there is anything else we should do, please do not hesitate to let us know. On behalf of my co-authors, we would like to express our great appreciation to editor and reviewers.

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Hao Jiang

As an author who has recently published in the journal "Brain and Neurological Disorders". I am delighted to provide a testimonial on the peer review process, editorial office support, and the overall quality of the journal. The peer review process at Brain and Neurological Disorders is rigorous and meticulous, ensuring that only high-quality, evidence-based research is published. The reviewers are experts in their fields, and their comments and suggestions were constructive and helped improve the quality of my manuscript. The review process was timely and efficient, with clear communication from the editorial office at each stage. The support from the editorial office was exceptional throughout the entire process. The editorial staff was responsive, professional, and always willing to help. They provided valuable guidance on formatting, structure, and ethical considerations, making the submission process seamless. Moreover, they kept me informed about the status of my manuscript and provided timely updates, which made the process less stressful. The journal Brain and Neurological Disorders is of the highest quality, with a strong focus on publishing cutting-edge research in the field of neurology. The articles published in this journal are well-researched, rigorously peer-reviewed, and written by experts in the field. The journal maintains high standards, ensuring that readers are provided with the most up-to-date and reliable information on brain and neurological disorders. In conclusion, I had a wonderful experience publishing in Brain and Neurological Disorders. The peer review process was thorough, the editorial office provided exceptional support, and the journal's quality is second to none. I would highly recommend this journal to any researcher working in the field of neurology and brain disorders.

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Dr Shiming Tang

Dear Agrippa Hilda, Journal of Neuroscience and Neurological Surgery, Editorial Coordinator, I trust this message finds you well. I want to extend my appreciation for considering my article for publication in your esteemed journal. I am pleased to provide a testimonial regarding the peer review process and the support received from your editorial office. The peer review process for my paper was carried out in a highly professional and thorough manner. The feedback and comments provided by the authors were constructive and very useful in improving the quality of the manuscript. This rigorous assessment process undoubtedly contributes to the high standards maintained by your journal.

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Raed Mualem

International Journal of Clinical Case Reports and Reviews. I strongly recommend to consider submitting your work to this high-quality journal. The support and availability of the Editorial staff is outstanding and the review process was both efficient and rigorous.

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Andreas Filippaios

Thank you very much for publishing my Research Article titled “Comparing Treatment Outcome Of Allergic Rhinitis Patients After Using Fluticasone Nasal Spray And Nasal Douching" in the Journal of Clinical Otorhinolaryngology. As Medical Professionals we are immensely benefited from study of various informative Articles and Papers published in this high quality Journal. I look forward to enriching my knowledge by regular study of the Journal and contribute my future work in the field of ENT through the Journal for use by the medical fraternity. The support from the Editorial office was excellent and very prompt. I also welcome the comments received from the readers of my Research Article.

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Dr Suramya Dhamija

Dear Erica Kelsey, Editorial Coordinator of Cancer Research and Cellular Therapeutics Our team is very satisfied with the processing of our paper by your journal. That was fast, efficient, rigorous, but without unnecessary complications. We appreciated the very short time between the submission of the paper and its publication on line on your site.

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Bruno Chauffert

I am very glad to say that the peer review process is very successful and fast and support from the Editorial Office. Therefore, I would like to continue our scientific relationship for a long time. And I especially thank you for your kindly attention towards my article. Have a good day!

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Baheci Selen

"We recently published an article entitled “Influence of beta-Cyclodextrins upon the Degradation of Carbofuran Derivatives under Alkaline Conditions" in the Journal of “Pesticides and Biofertilizers” to show that the cyclodextrins protect the carbamates increasing their half-life time in the presence of basic conditions This will be very helpful to understand carbofuran behaviour in the analytical, agro-environmental and food areas. We greatly appreciated the interaction with the editor and the editorial team; we were particularly well accompanied during the course of the revision process, since all various steps towards publication were short and without delay".

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Jesus Simal-Gandara

I would like to express my gratitude towards you process of article review and submission. I found this to be very fair and expedient. Your follow up has been excellent. I have many publications in national and international journal and your process has been one of the best so far. Keep up the great work.

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Douglas Miyazaki

We are grateful for this opportunity to provide a glowing recommendation to the Journal of Psychiatry and Psychotherapy. We found that the editorial team were very supportive, helpful, kept us abreast of timelines and over all very professional in nature. The peer review process was rigorous, efficient and constructive that really enhanced our article submission. The experience with this journal remains one of our best ever and we look forward to providing future submissions in the near future.

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Dr Griffith

I am very pleased to serve as EBM of the journal, I hope many years of my experience in stem cells can help the journal from one way or another. As we know, stem cells hold great potential for regenerative medicine, which are mostly used to promote the repair response of diseased, dysfunctional or injured tissue using stem cells or their derivatives. I think Stem Cell Research and Therapeutics International is a great platform to publish and share the understanding towards the biology and translational or clinical application of stem cells.

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Dr Tong Ming Liu

I would like to give my testimony in the support I have got by the peer review process and to support the editorial office where they were of asset to support young author like me to be encouraged to publish their work in your respected journal and globalize and share knowledge across the globe. I really give my great gratitude to your journal and the peer review including the editorial office.

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Husain Taha Radhi

I am delighted to publish our manuscript entitled "A Perspective on Cocaine Induced Stroke - Its Mechanisms and Management" in the Journal of Neuroscience and Neurological Surgery. The peer review process, support from the editorial office, and quality of the journal are excellent. The manuscripts published are of high quality and of excellent scientific value. I recommend this journal very much to colleagues.

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S Munshi

Dr.Tania Muñoz, My experience as researcher and author of a review article in The Journal Clinical Cardiology and Interventions has been very enriching and stimulating. The editorial team is excellent, performs its work with absolute responsibility and delivery. They are proactive, dynamic and receptive to all proposals. Supporting at all times the vast universe of authors who choose them as an option for publication. The team of review specialists, members of the editorial board, are brilliant professionals, with remarkable performance in medical research and scientific methodology. Together they form a frontline team that consolidates the JCCI as a magnificent option for the publication and review of high-level medical articles and broad collective interest. I am honored to be able to share my review article and open to receive all your comments.

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Tania Munoz

“The peer review process of JPMHC is quick and effective. Authors are benefited by good and professional reviewers with huge experience in the field of psychology and mental health. The support from the editorial office is very professional. People to contact to are friendly and happy to help and assist any query authors might have. Quality of the Journal is scientific and publishes ground-breaking research on mental health that is useful for other professionals in the field”.

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George Varvatsoulias

Dear editorial department: On behalf of our team, I hereby certify the reliability and superiority of the International Journal of Clinical Case Reports and Reviews in the peer review process, editorial support, and journal quality. Firstly, the peer review process of the International Journal of Clinical Case Reports and Reviews is rigorous, fair, transparent, fast, and of high quality. The editorial department invites experts from relevant fields as anonymous reviewers to review all submitted manuscripts. These experts have rich academic backgrounds and experience, and can accurately evaluate the academic quality, originality, and suitability of manuscripts. The editorial department is committed to ensuring the rigor of the peer review process, while also making every effort to ensure a fast review cycle to meet the needs of authors and the academic community. Secondly, the editorial team of the International Journal of Clinical Case Reports and Reviews is composed of a group of senior scholars and professionals with rich experience and professional knowledge in related fields. The editorial department is committed to assisting authors in improving their manuscripts, ensuring their academic accuracy, clarity, and completeness. Editors actively collaborate with authors, providing useful suggestions and feedback to promote the improvement and development of the manuscript. We believe that the support of the editorial department is one of the key factors in ensuring the quality of the journal. Finally, the International Journal of Clinical Case Reports and Reviews is renowned for its high- quality articles and strict academic standards. The editorial department is committed to publishing innovative and academically valuable research results to promote the development and progress of related fields. The International Journal of Clinical Case Reports and Reviews is reasonably priced and ensures excellent service and quality ratio, allowing authors to obtain high-level academic publishing opportunities in an affordable manner. I hereby solemnly declare that the International Journal of Clinical Case Reports and Reviews has a high level of credibility and superiority in terms of peer review process, editorial support, reasonable fees, and journal quality. Sincerely, Rui Tao.

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Rui Tao

Clinical Cardiology and Cardiovascular Interventions I testity the covering of the peer review process, support from the editorial office, and quality of the journal.

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Khurram Arshad

Clinical Cardiology and Cardiovascular Interventions, we deeply appreciate the interest shown in our work and its publication. It has been a true pleasure to collaborate with you. The peer review process, as well as the support provided by the editorial office, have been exceptional, and the quality of the journal is very high, which was a determining factor in our decision to publish with you.

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Gomez Barriga Maria Dolores

The peer reviewers process is quick and effective, the supports from editorial office is excellent, the quality of journal is high. I would like to collabroate with Internatioanl journal of Clinical Case Reports and Reviews journal clinically in the future time.

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Lin Shaw Chin

Clinical Cardiology and Cardiovascular Interventions, I would like to express my sincerest gratitude for the trust placed in our team for the publication in your journal. It has been a true pleasure to collaborate with you on this project. I am pleased to inform you that both the peer review process and the attention from the editorial coordination have been excellent. Your team has worked with dedication and professionalism to ensure that your publication meets the highest standards of quality. We are confident that this collaboration will result in mutual success, and we are eager to see the fruits of this shared effort.

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Maria Dolores Gomez Barriga

Dear Dr. Jessica Magne, Editorial Coordinator 0f Clinical Cardiology and Cardiovascular Interventions, I hope this message finds you well. I want to express my utmost gratitude for your excellent work and for the dedication and speed in the publication process of my article titled "Navigating Innovation: Qualitative Insights on Using Technology for Health Education in Acute Coronary Syndrome Patients." I am very satisfied with the peer review process, the support from the editorial office, and the quality of the journal. I hope we can maintain our scientific relationship in the long term.

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Dr Maria Dolores Gomez Barriga

Dear Monica Gissare, - Editorial Coordinator of Nutrition and Food Processing. ¨My testimony with you is truly professional, with a positive response regarding the follow-up of the article and its review, you took into account my qualities and the importance of the topic¨.

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Dr Maria Regina Penchyna Nieto

Dear Dr. Jessica Magne, Editorial Coordinator 0f Clinical Cardiology and Cardiovascular Interventions, The review process for the article “The Handling of Anti-aggregants and Anticoagulants in the Oncologic Heart Patient Submitted to Surgery” was extremely rigorous and detailed. From the initial submission to the final acceptance, the editorial team at the “Journal of Clinical Cardiology and Cardiovascular Interventions” demonstrated a high level of professionalism and dedication. The reviewers provided constructive and detailed feedback, which was essential for improving the quality of our work. Communication was always clear and efficient, ensuring that all our questions were promptly addressed. The quality of the “Journal of Clinical Cardiology and Cardiovascular Interventions” is undeniable. It is a peer-reviewed, open-access publication dedicated exclusively to disseminating high-quality research in the field of clinical cardiology and cardiovascular interventions. The journal's impact factor is currently under evaluation, and it is indexed in reputable databases, which further reinforces its credibility and relevance in the scientific field. I highly recommend this journal to researchers looking for a reputable platform to publish their studies.

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Dr Marcelo Flavio Gomes Jardim Filho

Dear Editorial Coordinator of the Journal of Nutrition and Food Processing! "I would like to thank the Journal of Nutrition and Food Processing for including and publishing my article. The peer review process was very quick, movement and precise. The Editorial Board has done an extremely conscientious job with much help, valuable comments and advices. I find the journal very valuable from a professional point of view, thank you very much for allowing me to be part of it and I would like to participate in the future!”

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Zsuzsanna Bene

Dealing with The Journal of Neurology and Neurological Surgery was very smooth and comprehensive. The office staff took time to address my needs and the response from editors and the office was prompt and fair. I certainly hope to publish with this journal again.Their professionalism is apparent and more than satisfactory. Susan Weiner

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Dr Susan Weiner

My Testimonial Covering as fellowing: Lin-Show Chin. The peer reviewers process is quick and effective, the supports from editorial office is excellent, the quality of journal is high. I would like to collabroate with Internatioanl journal of Clinical Case Reports and Reviews.

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Lin-Show Chin

My experience publishing in Psychology and Mental Health Care was exceptional. The peer review process was rigorous and constructive, with reviewers providing valuable insights that helped enhance the quality of our work. The editorial team was highly supportive and responsive, making the submission process smooth and efficient. The journal's commitment to high standards and academic rigor makes it a respected platform for quality research. I am grateful for the opportunity to publish in such a reputable journal.

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Sonila Qirko

My experience publishing in International Journal of Clinical Case Reports and Reviews was exceptional. I Come forth to Provide a Testimonial Covering the Peer Review Process and the editorial office for the Professional and Impartial Evaluation of the Manuscript.

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Luiz Sellmann