AUCTORES
Globalize your Research
Research Article
*Corresponding Author: Ali Souldozi, Department of Chemistry, Urmia Branch, Islamic Azad University, Urmia, Iran.
Citation: Naeimeh Nazari, Ali Souldozi, (2023), Synthesis and Evaluation of Antibacterial Activity of Novel Disubstituted 1,3,4-Oxadiazoles Derivatives Containing Fluorine Substituent, J, Biotechnology and Bioprocessing, 4(3); DOI: 10.31579/2766-2314/102
Copyright: 2023, Ali Souldozi. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Received: 29 May 2023 | Accepted: 07 June 2023 | Published: 15 June 2023
Keywords: N-isocyaniminotriphenylphosphorane; 1,3,4-oxadiazoles; antibacterial activity
In the present study a new series of antibacterial agents (4a-f) was designed, synthesized and evaluated for antibacterial activity against different gram positive (Staphylococcusaureus, Staphylococcus epidermidis, Bacillus cereus) and gram negative (listeria monocytogenes, Escherichia coli, Pseudomonas aeroginosa) bacteria. New derivatives of oxadiazoles were prepared by one-pot reaction of N-isocyanoiminotriphenylphosphorane, carboxylic acid derivatives and 2-pyridinecarboxaldehyde, in the presence of acetonitrile solvent. The broth macro dilution and well agar diffusion methods were used for determination of inhibition zone (IZ) and minimum inhibitory concentration (MIC) during preliminary evaluation of antimicrobial activity. The (MIC) values of tested compounds revealed that all compounds have displayed significant antibacterial activity against Staphylococcusaureus and Staphylococcus epidermidis (with MIC values in the range of 31 to 125µg/mL).
Widespread antibiotic resistance appeared over the last 60 years and generates a serious challenge to the medical pundits. Despite the presence of antibiotics in structural and vaccination programmes, infectious diseases are major causes of morbidity and mortality worldwide. Thus, there is essential need to novel insights into development of drugs with new mechanisms of action. Among the wide variety of heterocycles that have been explored for developing new therapeutic molecules, 1,3,4-oxadiazoles become promising moiety in the structure of drugs [1,2]. 1,3,4-Oxadiazole are known to show antibacterial [3,4], antifungal [5], analgesic [6], anti-inflammator [7,8], antitubercular [9], pesticidal [10]. anti-HIV [11]and anti-cancer [12]. The improvement in activity due to the presence of aromatic moiety in the molecules that increases the lipophilicity and affects the subdividing of molecules into membranes and comfort hydrophobic interactions of the molecules with specific binding sites on either receptor or enzymes is well established [13]. In recent years, a number of new 1,3,4-oxadiazole derivatives as antibacterial agents have been reported. The main modifications of these compounds were focused on triflouromethyl and flourophenyl groups [14]. In this research, we designed and synthesized new series of fluorinated 1,3,4-oxadiazoles derivatives and evaluated there in vitro antibacterial activity against against some pathogenic bacteria.
2.1 Materials and Methods
Chemistry 1H (300.13 MHz) and 13C (75.47 MHz) NMR measurements were recorded on a Bruker 250 spectrometer in CDCl3 with tetramethylsilane as internal standard. IR spectra were measured on a Shimadzu IR-460 spectrometer. Melting points were measured on an Electrothermal 9100 apparatus and are uncorrected. N-isocyanoiminotriphenylphosphorane 2 was prepared based on a reported procedure [15,16]. Other starting materials and solvents were obtained from Merck (Germany) and Fluka (Switzerland) and were used without further purification. Flash chromatography columns were prepared from Merck silica gel powder. The structures of the products were deduced from their IR, 1H NMR and 13C NMR spectra.
2.2 General Procedure for the Preparation of products (4a-f)
To a magnetically stirred solution of N-isocyanoiminotriphenylphosphorane (1) (0.302 g, 1 mmol) in CH3CN (8 ml) was added drop-wise a solution of 2(1 mmol) in CH3CN (7 ml) over 15 min. The mixture was stirred for 12 h at room temperature. The solvent was removed under reduced pressure and the viscous residue was purified by flash column chromatography (silica gel; petroleum ether–ethyl acetate (10:3). The solvent was removed under reduced pressure and the products (4a–f) were obtained.
(5-(2-fluorophenyl)-1,3,4-oxadiazol-2-yl) (pyridin-2-yl) methanol (4a)
White powder, m.p. 122 ºC, yield 74%
IR (KBr) (νmax , Cm-1): 3416, 3064, 2927, 1685, 1609, 1491, 1437, 1191, 1117, 722.
1HNMR (300.13 MHz, CDCl3): δH= 6.24 (s, CH, Aliphatic), 6.63 (s, 1H, OH), 7.31-7.37 (m, 2H, CH, Ar), 7.64-7.67 (m, 1H, CH, Ar), 7.88-7.91 (m, 1H, CH, Ar), 7.93-8.01(m, 1H, CH, Pyridine ring), 8.23-8.26 (m, 1H, CH, Pyridine ring), 8.47-8.51 (m, 1H, CH, Pyridine ring), 8.88-8.93 (m, 1H, CH, Pyridine ring ).
13CNMR (75.467 MHz, CDCl3): δC= 66.7 (CH-OH), 116.8 (1C, d, 2JCF= 22.3 Hz, Ar), 119.4 (1C, d, 2JCF= 10.4 Hz, Ar), 124.4 (1CH, d, 4JCF= 4.1 Hz, C Ar), 131.8 (1CH, d, 3JCF= 1.4 Hz, Ar), 134.6 (1CH, d, 3JCF= 9.0 Hz, Ar), 124.9, 128.4, 137.5, 150.5 (4 CH Pyridine ring), 155.4 and 166.8 (1C, d, 1JCF= 256.8 Hz, Ar), 158.3 (1C, Pyridine ring), 161.9 (1C, oxadiazol ring), 165.1 and 164.1 (1C, d, 3JCF= 22.9 Hz, oxadiazol ring).
(5-(3-fluorophenyl)-1,3,4-oxadiazol-2-yl) (pyridin-2-yl) methanol (4b)
White powder, m.p. 125 ºC, yield 71%.
IR (KBr) (νmax , Cm-1): 3435 (br), 3055, 2924, 2855, 1677, 1549, 1463, 1370, 1216, 1180, 1091, 998.
1HNMR (300.13 MHz, CDCl3): δH= 6.16 (s, 1H, CH aliphatic), 6.54 (s, 1H, OH), 7.31-7.36 (m, 1H, CH, Ar), 7.54-7.64 (m, 1H, CH, Ar), 7.64-7.67 (m, 1H, CH, Ar), 7.94-7.98 (m, 2H, CH, Ar), 8.01-8.06 (m, 1H, CH, Pyridine ring), 8.46 (d, 1H, 3JHH= 8.1 Hz, CH, Pyridine ring), 8.92-8.93 (m, 1H, CH Pyridine ring).
13CNMR (75.467 MHz, CDCl3): δC= 66.8 (CH-OH), 114.9 (d, 1H, 2JCF= 24.4 Hz, CH Ar), 120.0 (d, 1CH, 2JCF= 21.2 Hz, Ar), 123.7 (d, 1C, 3JFC= 3.4 Hz, Ar), 126.1 (d, 1CH, 4JFC= 3.2 Hz, Ar), 125.7, 128.2, 137.3, 150.2 (4 CH Pyridine ring), 157.7 and 167.5 (d, 1C, 1JFC= 352.2 Hz, Ar), 158.2 (1C, Pyridine ring), 161.7, 166.8 (2C).
(5-(4-fluorophenyl)-1,3,4-oxadiazol-2-yl) (pyridin-2-yl) methanol (4c)
White powder, m.p. 128 ºC, yield 77%
IR (KBr) (νmax , Cm-1):3406, 3057, 2928, 1677, 1607, 1491, 1438, 1185, 1115, 716.
1HNMR (300.13 MHz, CDCl3): δH= 6.23 (s, CH, Aliphatic), 6.61 (s, 1H, OH), 7.47-7.48 (m, 2H, CH, Ar), 7.64-7.71 (m, 2H, CH, Ar), 7.53-7.55 (m, 1H, CH, Pyridine ring), 7.99-8.04 (m, 1H, CH Ar), 8.42-8.45 (d, 1H,3JHH= 7.5 Hz, CH Ar), 8.89-8.91 (m, 1H, CH Pyridine ring).
13CNMR (75.467 MHz, CDCl3): δC= 67.0 (CH-OH), 115.5 (2CH, d, 2JCF= 22.1 Hz, CH Ar), 132.0 (2CH, d, 3JCF= 9.0 Hz, CH Ar), 162.4 and 167.4 (1C, d, 1JCF= 250.4 Hz, C Ar), 127.4 (1C Ar), 125.7, 128.2, 137.2, 150.2 (4 CH Pyridine ring), 158.2 (1C, Pyridine ring), 161.7, 166.8 (2C).
(5-(3,4-difluorophenyl)-1,3,4-oxadiazol-2-yl) (pyridin-2-yl) methanol (4d)
White powder, m.p. 140 ºC, yield 81%
IR (KBr) (νmax , Cm-1): 3412, 3062, 2926, 1683, 1601, 1486, 1431, 1185, 1112, 718.
1HNMR (300.13 MHz, CDCl3): δH= 6.20 (s, CH, Aliphatic), 6.61 (s, 1H, OH), 7.46-7,54 (m, 2H, Ar), 7.64-7.71 (m, 1H, Ar), 7.96-8.01 (m, 1H, Pyridine ring), 8.21-8.25 (m, 1H, Pyridine ring), 8.44-8.46 (m, 1H, Pyridine ring), 8.87-8.91 (m, 1H, Pyridine ring).
13CNMR (75.467 MHz, CDCl3): δC= 66.8 (CH-OH), 116.7 (1CH, d, 3JCF= 22.6 Hz, CH Ar), 119.2-120.1 (1CH, d of d, 3JCF= 2.0 Hz, 2JCF= 16.9 Hz, CH Ar), 126.1-126.5 (1CH, d of d, 4JCF= 2.3 Hz, 3JCF= 3.5 Hz, CH Ar), 127.4 (1CH , q, 3JCF= 3.8 Hz, CH Ar), 149.0-149.5 and 155.0-155.5 (1CF, d of d, 2JCF= 12.8 Hz, 1JCF= 257.9 Hz), 145.0-145.6 and 155.0-155.5 (1CF, d of d, 2JCF= 12.8 Hz, 1JCF= 257.9 Hz, CF Arom), 124.7, 128.2, 137.3, 150.2 (4CH, Pyridine ring), 158.3(1C, Pyridine ring), 161.8, 166.8 (2C).
pyridin-2-yl(5-(3-(trifluoromethyl) phenyl)-1,3,4-oxadiazol-2-yl) methanol (4e)
White powder, m.p. 143 ºC, yield 84%
IR (KBr) (νmax , Cm-1): 3408, 3062, 2931, 1678, 1610, 1497, 1442, 1190, 1118, 718.
1HNMR (300.13 MHz, CDCl3): δH= 6.21 (s, CH, Aliphatic), 6.64 (s, 1H, OH), 7.61-7.64 (m, 1H, CH, Ar), 7.81-7.88 (m, 1H, CH, Ar), 8.34-8.38 (m, 2H, CH, Ar), 7.95-7.99 (m, 1H, CH, Pyridine ring), 8.03-8.08 (m, 1H, CH, Pyridine ring), 8.45 (1H, d, 3JHH= 8 Hz, Pyridine ring), 8.94-8.97 (m, 1H, CH, Pyridine ring).
13CNMR (75.467 MHz, CDCl3): δC= 66.9 (CH-OH), 107.4, 118.3, 129.1, 139.9 (1C, q, 1JCF= 272.2 Hz, Ar), 127.3 (1CH, q, 3JCF= 3.8 Hz, Ar), 129.5, 130.8, 132.1, 133.5 (1C, q, 2JCF= 33.2 Hz, Ar), 129.3, 133.5 (2CH, Ar), 130.8 (1C, Ar), 130.5 (1CH, q, 3JCF= 3.8 Hz, Ar), 125 .8, 128.4, 137.4, 150.3 (4 CH, Pyridine ring), 158.4 (1C, Pyridine ring), 161.8, 166.9 (2C, Oxadiazol ring).
pyridin-2-yl(5-(4-(trifluoromethyl) phenyl)-1,3,4-oxadiazol-2-yl) methanol(4f)
White powder, m.p. 146 ºC, yield 85%
IR (KBr) (νmax , Cm-1): 3412, 3071, 2931, 1679, 1610, 1496, 1444, 1189, 1117, 715.
1HNMR (300.13 MHz, CDCl3): δH= 6.22 (s, CH, Aliphatic), 6.62 (s, 1H, OH), 7.88 (2H, d, 3JHH= 8 Hz, Ar), 8.17 (2H, d, 3JHH= 8.0 Hz, Ar), 7.94-8.01(m, 1H, CH, Pyridine ring), 8.03-8.09 (m, 1H, CH, Pyridine ring), 8.47 (1H, d, 3JHH= 8.0 Hz, Pyridine ring), 8.87-8.92 (m, 1H, CH, Pyridine ring).
13CNMR (75.467 MHz, CDCl3): δC= 67.0 (CH-OH), 125.5 (2CH, q, 3JCF= 3.6 Hz, Ar), 130.1 (2CH, Ar), 130.5, 131.9, 133.3, 134.8 (1C, q, 2JCF= 32.2 Hz, Ar), 105.7, 117.8, 129.9, 142.0 (1C, q, 1JCF= 272.6 Hz, Ar), 134.8 (1C, Ar), 125.7, 128.3, 137.2, 150.3 (4 CH Pyridine ring), 158.4 (1C, Pyridine ring), 161.9, 166.9 (2C, Oxadiazol ring).
3.1 Agar well diffusion method
The antibacterial activity of synthesized compounds was investigated against Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, listeria monocytogenes ATCC 23074, Pseudomonas aeroginosa ATCC 27853, Bacillus cereus ATCC 11778, Staphylococcus epidermidis ATCC 14990 by agar well diffusion and broth dilution methods [15-16].
The standardization of each bacterial inoculum was done following the following them to Clinical & Laboratory Standards Institute (CLSI) [17]. Briefly, each microorganism after inoculation into Mueller Hinton broth (Merck, Germany) was prepared to turbidity standard of 0.5 McFarland. The prepared suspension from tested bacteria was transferred on solidified agar and spreaded uniformly. Synthetic antibacterial agents were dissolved in dimethyl sulfoxide (DMSO) to get stock with concentration of 1mg/ml [18,19]. After punching wells in agar using a sterile glass tube, 70µl of compounds and DMSO were transferred to each well. The zones around each well against defined bacteria after incubation for 24h, determinated the antibacterial power of each compound [20]. Ceftizoxime, Ciprofloxacin and DMSO were used with the same method as positive control and negative control respectively.
3.2 Broth dilution Method
In the next step for determination the lowest concentration of an antimicrobial agents that inhibits the visible growth of the tested microorganism (MIC value), broth dilution method was used [21]. In this study, eight sterile tubes were used for each compound and after transferring the broth culture medium into the tubes, dilution was done and 10µL of inoculum contained 1.5×106 C.F.U/ml of tested microorganism was added to each test tubes. After the incubation, the tubes were checked for the minimum inhibitory concentration concentration point. Ceftizixime, ciprofloxacin and DMSO respectively were also observed for comparative tests. Minimum Bactericidal Concentration (MBC) values were determined by subculturing of the tested tubes on agar media that show no visible bacterial growth [22].
3.3 Statistical analysis
We used Excel to do some basic data on three independent experiments,and the results are expressed as mean ± SEM.
4.1 Chemistry
We found that 3-fluorobenzoic acid derivatives 3a reacted with 2-pyridinecarbaldehyde 2 and N-isocyanoiminotriphenylphosphorane 1 in CH3CN react in a 1:1:1 ratio at room temperature to produce (5-(3-fluorophenyl)-1,3,4-oxadiazol-2-yl) (pyridin-2-yl) methanol 4a and, as a by-product, Ph3P=O 5 (Scheme 1). The reaction proceeded smoothly and cleanly under mild conditions in 74% yield, and no side reactions were observed. The other aromatic carboxylic acids also reacted smoothly to give similar products 4b-4f in yields of 71, 85% (Scheme1). We tried using the simple ketone analogous, 1-(2-pyridyl)-1-ethanone instead of 2-pyridinecarbaldehyde 2 in this reaction, but no corresponding products of type 4 were observed (Scheme1).
Scheme 1: Synthesis of (5-(fluoroaryl)-1,3,4-oxadiazol-2-yl) (pyridin-2-yl) methanol (4a-f)
The structures of the products were deduced from their IR, 1H and 13C NMR data. The 1H-NMR spectrum of 4a consisted of a singlet for CH aliphatic (δ= 6.24 ppm), a singlet for OH (δ= 6.63 ppm), exchangeable by D2O and multiple at δ= 7.31-8.93 ppm for the aromatic hydrogen atoms. The 1H decoupled 13C NMR spectrum of 4a showed eleven distinct resonances, partial assignment of these resonances is given in the experimental. The 1H and 13C NMR spectra of compounds 4b- 4f were similar to those of 4a, except for the aromatic moiety, which exhibited characteristic signals with appropriate chemical shifts.
A mechanistic rationalization for this reaction is depicted in Scheme 2. On the basis of the chemistry of isocyanides, it is reasonable to assume that the first step involves nucleophilic addition of 1 to 2-pyridinecarbaldehyde 2, facilitated by its protonation with the acid 3, leading to nitrilium intermediate 6. This intermediate may be attacked by the conjugate base of the acid 7 to form the 1:1:1 adduct 8. This adduct may undergo an intramolecular aza-Wittig reaction of the iminophosphorane moiety with the ester C=O group to afford the isolated (5-(fluoroaryl)-1,3,4-oxadiazol-2-yl) (pyridin-2-yl) methanol 4 by removal of Ph3P=O 5 from intermediate 9. In this reaction, the first two reaction steps are analogous to the well-known Passerini reaction, and the final step is analogous to the well-known intramolecularaza-Wittig reaction (tandem Passerini/intramolecularaza-Wittig sequence) (Scheme 2).
Scheme 2: Plausible mechanism for the (5-(fluoroaryl)-1,3,4-oxadiazol-2-yl) (pyridin-2-yl) methanol
4-2 Measurement of antimicrobial activity using Agar well diffusion Method
The antimicrobial activity of the synthesized derivatives was measured by zone of inhibition around each well. With comparison of zone of inhibition diameters of synthetic derivatives with Ceftizoxime and Ciprofloxacin drugs, the antibacterial power of oxadiazole derivatives were determined.
As shown in Figure 1 all the Oxadiazole derivatives that were synthesized in this research show significant anti actibacterial activity against Staphylococcus aureus with IZ of 16-21, whereas an IZ of 21-24mm was observed against Staphylococcus epidermidis and their antibacterial activity are notably more in compare with Ceftizoxime. No zone of inhibition was seen around the well containing DMSO (Negative control). (Figure1 and Table1).
Figure 1: Inhibition zone of compounds against Staphylococcus aureus and Staphylococcus epidermidis in 1mg/ml
NA:No ActivityThe result are expressed as mean ±sem*
Table1: Antibacterial activity of (5-(fluoroaryl)-1,3,4-oxadiazol-2-yl) (pyridin-2-yl) methanol derivatives by Agar well diffusion method (1mg/ml)
NA:No Activity The result are expressed as mean ±sem*
Table2: Antibacterial activity of (5-(fluoroaryl)-1,3,4-oxadiazol-2-yl) (pyridin-2-yl) methanol derivatives by broth dilution method(µg/mL)
The minimum inhibitory concentrations (MIC) of tested compounds (4a-f) are shown in (Table2). The MBC of compounds were three-fold higher than the corresponding MIC results. Compounds 4f were the most effective against S.aureus and S.epidermidis with MIC values of 15µg/mL and 31.25μg/mL respectively. while the MIC of the compounds 4f containing 4-trifluoromethylphenyl, were determined in the range of 31 to 62 µg/ml against S.aureus and S.epidermidis.
The investigation of antibacterial screening data revealed that all the tested compounds (4a-f) showed moderate to good inhibition at μg/ml in DMSO. This report intends to explore the role of electronic environment (fluorine atoms) on antibacterial activity. In our study, ring substitution by fluorine increased the antibacterial activity of the compounds in compared to compounds of our previous research [23]. The data found in the literature matching our research and claims that the compounds with halogen substituent are the most efficient against Gram-positive bacteria, particularly against S. aureus [24, 25]. In the course of this study, derivativs 4a-f possessing pyridine ring at the C-2 position on the linker of 1,3,4-oxadiazoles was identified as showing moderately enhanced antibacterial activity against gram positive bacteria as compared to our previous report so interesting activity of compounds 4a-f against Staphylococcus aureus and Staphylococcus epidermidis, which is much stronger than that seen against Gram negative bacteria can be correlated to this lipophilic group [26]. A limited effect is detected for the compound against Escherichia coli and Pseudomonas aeroginosa (MICs 500-1000 μg mL).
As in the presented results, the compounds had good antibacterial power, but in addition to the antimicrobial power of the synthesized derivatives, the presented one-step synthesis method can also be a very suitable method for the synthesis of derivatives with high antibacterial power and low financial and time cost.
This work was supported by Islamic Azad University, Urmia Branch.
Clearly Auctoresonline and particularly Psychology and Mental Health Care Journal is dedicated to improving health care services for individuals and populations. The editorial boards' ability to efficiently recognize and share the global importance of health literacy with a variety of stakeholders. Auctoresonline publishing platform can be used to facilitate of optimal client-based services and should be added to health care professionals' repertoire of evidence-based health care resources.
Journal of Clinical Cardiology and Cardiovascular Intervention The submission and review process was adequate. However I think that the publication total value should have been enlightened in early fases. Thank you for all.
Journal of Women Health Care and Issues By the present mail, I want to say thank to you and tour colleagues for facilitating my published article. Specially thank you for the peer review process, support from the editorial office. I appreciate positively the quality of your journal.
Journal of Clinical Research and Reports I would be very delighted to submit my testimonial regarding the reviewer board and the editorial office. The reviewer board were accurate and helpful regarding any modifications for my manuscript. And the editorial office were very helpful and supportive in contacting and monitoring with any update and offering help. It was my pleasure to contribute with your promising Journal and I am looking forward for more collaboration.
We would like to thank the Journal of Thoracic Disease and Cardiothoracic Surgery because of the services they provided us for our articles. The peer-review process was done in a very excellent time manner, and the opinions of the reviewers helped us to improve our manuscript further. The editorial office had an outstanding correspondence with us and guided us in many ways. During a hard time of the pandemic that is affecting every one of us tremendously, the editorial office helped us make everything easier for publishing scientific work. Hope for a more scientific relationship with your Journal.
The peer-review process which consisted high quality queries on the paper. I did answer six reviewers’ questions and comments before the paper was accepted. The support from the editorial office is excellent.
Journal of Neuroscience and Neurological Surgery. I had the experience of publishing a research article recently. The whole process was simple from submission to publication. The reviewers made specific and valuable recommendations and corrections that improved the quality of my publication. I strongly recommend this Journal.
Dr. Katarzyna Byczkowska My testimonial covering: "The peer review process is quick and effective. The support from the editorial office is very professional and friendly. Quality of the Clinical Cardiology and Cardiovascular Interventions is scientific and publishes ground-breaking research on cardiology that is useful for other professionals in the field.
Thank you most sincerely, with regard to the support you have given in relation to the reviewing process and the processing of my article entitled "Large Cell Neuroendocrine Carcinoma of The Prostate Gland: A Review and Update" for publication in your esteemed Journal, Journal of Cancer Research and Cellular Therapeutics". The editorial team has been very supportive.
Testimony of Journal of Clinical Otorhinolaryngology: work with your Reviews has been a educational and constructive experience. The editorial office were very helpful and supportive. It was a pleasure to contribute to your Journal.
Dr. Bernard Terkimbi Utoo, I am happy to publish my scientific work in Journal of Women Health Care and Issues (JWHCI). The manuscript submission was seamless and peer review process was top notch. I was amazed that 4 reviewers worked on the manuscript which made it a highly technical, standard and excellent quality paper. I appreciate the format and consideration for the APC as well as the speed of publication. It is my pleasure to continue with this scientific relationship with the esteem JWHCI.
This is an acknowledgment for peer reviewers, editorial board of Journal of Clinical Research and Reports. They show a lot of consideration for us as publishers for our research article “Evaluation of the different factors associated with side effects of COVID-19 vaccination on medical students, Mutah university, Al-Karak, Jordan”, in a very professional and easy way. This journal is one of outstanding medical journal.
Dear Hao Jiang, to Journal of Nutrition and Food Processing We greatly appreciate the efficient, professional and rapid processing of our paper by your team. If there is anything else we should do, please do not hesitate to let us know. On behalf of my co-authors, we would like to express our great appreciation to editor and reviewers.
As an author who has recently published in the journal "Brain and Neurological Disorders". I am delighted to provide a testimonial on the peer review process, editorial office support, and the overall quality of the journal. The peer review process at Brain and Neurological Disorders is rigorous and meticulous, ensuring that only high-quality, evidence-based research is published. The reviewers are experts in their fields, and their comments and suggestions were constructive and helped improve the quality of my manuscript. The review process was timely and efficient, with clear communication from the editorial office at each stage. The support from the editorial office was exceptional throughout the entire process. The editorial staff was responsive, professional, and always willing to help. They provided valuable guidance on formatting, structure, and ethical considerations, making the submission process seamless. Moreover, they kept me informed about the status of my manuscript and provided timely updates, which made the process less stressful. The journal Brain and Neurological Disorders is of the highest quality, with a strong focus on publishing cutting-edge research in the field of neurology. The articles published in this journal are well-researched, rigorously peer-reviewed, and written by experts in the field. The journal maintains high standards, ensuring that readers are provided with the most up-to-date and reliable information on brain and neurological disorders. In conclusion, I had a wonderful experience publishing in Brain and Neurological Disorders. The peer review process was thorough, the editorial office provided exceptional support, and the journal's quality is second to none. I would highly recommend this journal to any researcher working in the field of neurology and brain disorders.
Dear Agrippa Hilda, Journal of Neuroscience and Neurological Surgery, Editorial Coordinator, I trust this message finds you well. I want to extend my appreciation for considering my article for publication in your esteemed journal. I am pleased to provide a testimonial regarding the peer review process and the support received from your editorial office. The peer review process for my paper was carried out in a highly professional and thorough manner. The feedback and comments provided by the authors were constructive and very useful in improving the quality of the manuscript. This rigorous assessment process undoubtedly contributes to the high standards maintained by your journal.
International Journal of Clinical Case Reports and Reviews. I strongly recommend to consider submitting your work to this high-quality journal. The support and availability of the Editorial staff is outstanding and the review process was both efficient and rigorous.
Thank you very much for publishing my Research Article titled “Comparing Treatment Outcome Of Allergic Rhinitis Patients After Using Fluticasone Nasal Spray And Nasal Douching" in the Journal of Clinical Otorhinolaryngology. As Medical Professionals we are immensely benefited from study of various informative Articles and Papers published in this high quality Journal. I look forward to enriching my knowledge by regular study of the Journal and contribute my future work in the field of ENT through the Journal for use by the medical fraternity. The support from the Editorial office was excellent and very prompt. I also welcome the comments received from the readers of my Research Article.
Dear Erica Kelsey, Editorial Coordinator of Cancer Research and Cellular Therapeutics Our team is very satisfied with the processing of our paper by your journal. That was fast, efficient, rigorous, but without unnecessary complications. We appreciated the very short time between the submission of the paper and its publication on line on your site.
I am very glad to say that the peer review process is very successful and fast and support from the Editorial Office. Therefore, I would like to continue our scientific relationship for a long time. And I especially thank you for your kindly attention towards my article. Have a good day!
"We recently published an article entitled “Influence of beta-Cyclodextrins upon the Degradation of Carbofuran Derivatives under Alkaline Conditions" in the Journal of “Pesticides and Biofertilizers” to show that the cyclodextrins protect the carbamates increasing their half-life time in the presence of basic conditions This will be very helpful to understand carbofuran behaviour in the analytical, agro-environmental and food areas. We greatly appreciated the interaction with the editor and the editorial team; we were particularly well accompanied during the course of the revision process, since all various steps towards publication were short and without delay".
I would like to express my gratitude towards you process of article review and submission. I found this to be very fair and expedient. Your follow up has been excellent. I have many publications in national and international journal and your process has been one of the best so far. Keep up the great work.
We are grateful for this opportunity to provide a glowing recommendation to the Journal of Psychiatry and Psychotherapy. We found that the editorial team were very supportive, helpful, kept us abreast of timelines and over all very professional in nature. The peer review process was rigorous, efficient and constructive that really enhanced our article submission. The experience with this journal remains one of our best ever and we look forward to providing future submissions in the near future.
I am very pleased to serve as EBM of the journal, I hope many years of my experience in stem cells can help the journal from one way or another. As we know, stem cells hold great potential for regenerative medicine, which are mostly used to promote the repair response of diseased, dysfunctional or injured tissue using stem cells or their derivatives. I think Stem Cell Research and Therapeutics International is a great platform to publish and share the understanding towards the biology and translational or clinical application of stem cells.
I would like to give my testimony in the support I have got by the peer review process and to support the editorial office where they were of asset to support young author like me to be encouraged to publish their work in your respected journal and globalize and share knowledge across the globe. I really give my great gratitude to your journal and the peer review including the editorial office.
I am delighted to publish our manuscript entitled "A Perspective on Cocaine Induced Stroke - Its Mechanisms and Management" in the Journal of Neuroscience and Neurological Surgery. The peer review process, support from the editorial office, and quality of the journal are excellent. The manuscripts published are of high quality and of excellent scientific value. I recommend this journal very much to colleagues.
Dr.Tania Muñoz, My experience as researcher and author of a review article in The Journal Clinical Cardiology and Interventions has been very enriching and stimulating. The editorial team is excellent, performs its work with absolute responsibility and delivery. They are proactive, dynamic and receptive to all proposals. Supporting at all times the vast universe of authors who choose them as an option for publication. The team of review specialists, members of the editorial board, are brilliant professionals, with remarkable performance in medical research and scientific methodology. Together they form a frontline team that consolidates the JCCI as a magnificent option for the publication and review of high-level medical articles and broad collective interest. I am honored to be able to share my review article and open to receive all your comments.
“The peer review process of JPMHC is quick and effective. Authors are benefited by good and professional reviewers with huge experience in the field of psychology and mental health. The support from the editorial office is very professional. People to contact to are friendly and happy to help and assist any query authors might have. Quality of the Journal is scientific and publishes ground-breaking research on mental health that is useful for other professionals in the field”.
Dear editorial department: On behalf of our team, I hereby certify the reliability and superiority of the International Journal of Clinical Case Reports and Reviews in the peer review process, editorial support, and journal quality. Firstly, the peer review process of the International Journal of Clinical Case Reports and Reviews is rigorous, fair, transparent, fast, and of high quality. The editorial department invites experts from relevant fields as anonymous reviewers to review all submitted manuscripts. These experts have rich academic backgrounds and experience, and can accurately evaluate the academic quality, originality, and suitability of manuscripts. The editorial department is committed to ensuring the rigor of the peer review process, while also making every effort to ensure a fast review cycle to meet the needs of authors and the academic community. Secondly, the editorial team of the International Journal of Clinical Case Reports and Reviews is composed of a group of senior scholars and professionals with rich experience and professional knowledge in related fields. The editorial department is committed to assisting authors in improving their manuscripts, ensuring their academic accuracy, clarity, and completeness. Editors actively collaborate with authors, providing useful suggestions and feedback to promote the improvement and development of the manuscript. We believe that the support of the editorial department is one of the key factors in ensuring the quality of the journal. Finally, the International Journal of Clinical Case Reports and Reviews is renowned for its high- quality articles and strict academic standards. The editorial department is committed to publishing innovative and academically valuable research results to promote the development and progress of related fields. The International Journal of Clinical Case Reports and Reviews is reasonably priced and ensures excellent service and quality ratio, allowing authors to obtain high-level academic publishing opportunities in an affordable manner. I hereby solemnly declare that the International Journal of Clinical Case Reports and Reviews has a high level of credibility and superiority in terms of peer review process, editorial support, reasonable fees, and journal quality. Sincerely, Rui Tao.
Clinical Cardiology and Cardiovascular Interventions I testity the covering of the peer review process, support from the editorial office, and quality of the journal.
Clinical Cardiology and Cardiovascular Interventions, we deeply appreciate the interest shown in our work and its publication. It has been a true pleasure to collaborate with you. The peer review process, as well as the support provided by the editorial office, have been exceptional, and the quality of the journal is very high, which was a determining factor in our decision to publish with you.
The peer reviewers process is quick and effective, the supports from editorial office is excellent, the quality of journal is high. I would like to collabroate with Internatioanl journal of Clinical Case Reports and Reviews journal clinically in the future time.
Clinical Cardiology and Cardiovascular Interventions, I would like to express my sincerest gratitude for the trust placed in our team for the publication in your journal. It has been a true pleasure to collaborate with you on this project. I am pleased to inform you that both the peer review process and the attention from the editorial coordination have been excellent. Your team has worked with dedication and professionalism to ensure that your publication meets the highest standards of quality. We are confident that this collaboration will result in mutual success, and we are eager to see the fruits of this shared effort.
Dear Dr. Jessica Magne, Editorial Coordinator 0f Clinical Cardiology and Cardiovascular Interventions, I hope this message finds you well. I want to express my utmost gratitude for your excellent work and for the dedication and speed in the publication process of my article titled "Navigating Innovation: Qualitative Insights on Using Technology for Health Education in Acute Coronary Syndrome Patients." I am very satisfied with the peer review process, the support from the editorial office, and the quality of the journal. I hope we can maintain our scientific relationship in the long term.
Dear Monica Gissare, - Editorial Coordinator of Nutrition and Food Processing. ¨My testimony with you is truly professional, with a positive response regarding the follow-up of the article and its review, you took into account my qualities and the importance of the topic¨.
Dear Dr. Jessica Magne, Editorial Coordinator 0f Clinical Cardiology and Cardiovascular Interventions, The review process for the article “The Handling of Anti-aggregants and Anticoagulants in the Oncologic Heart Patient Submitted to Surgery” was extremely rigorous and detailed. From the initial submission to the final acceptance, the editorial team at the “Journal of Clinical Cardiology and Cardiovascular Interventions” demonstrated a high level of professionalism and dedication. The reviewers provided constructive and detailed feedback, which was essential for improving the quality of our work. Communication was always clear and efficient, ensuring that all our questions were promptly addressed. The quality of the “Journal of Clinical Cardiology and Cardiovascular Interventions” is undeniable. It is a peer-reviewed, open-access publication dedicated exclusively to disseminating high-quality research in the field of clinical cardiology and cardiovascular interventions. The journal's impact factor is currently under evaluation, and it is indexed in reputable databases, which further reinforces its credibility and relevance in the scientific field. I highly recommend this journal to researchers looking for a reputable platform to publish their studies.
Dear Editorial Coordinator of the Journal of Nutrition and Food Processing! "I would like to thank the Journal of Nutrition and Food Processing for including and publishing my article. The peer review process was very quick, movement and precise. The Editorial Board has done an extremely conscientious job with much help, valuable comments and advices. I find the journal very valuable from a professional point of view, thank you very much for allowing me to be part of it and I would like to participate in the future!”
Dealing with The Journal of Neurology and Neurological Surgery was very smooth and comprehensive. The office staff took time to address my needs and the response from editors and the office was prompt and fair. I certainly hope to publish with this journal again.Their professionalism is apparent and more than satisfactory. Susan Weiner
