AUCTORES
Globalize your Research
Research Article
*Corresponding Author: Robert Germain Beka. Department of Food Science and Nutrition, ENSAI, University of Ngaoundere, Cameroon.
Citation: Emmanuel A. Panyoo, Robert G. Beka, Yadang G., Homsi P., Laurette B. M. Kenfack b and Tchiegang C., (2021) Production and characterization of exopolysaccharides from loss cooked sweet potatoes (Ipomoea batatas) by Lactobacillus plantarum J, Biotechnology and Bioprocessing 2(7); DOI: 10.31579/2766-2314/030
Copyright: © 2021, Robert Germain Beka. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Received: 07 March 2021 | Accepted: 29 March 2021 | Published: 20 August 2021
Keywords: expolysaccharides; fermentation; Lactobacillus; yield; titrable acidity
The aim of the work was to produce and characterize the exopolysaccharides from loss cooked sweet potato (Ipomoea batatas) using fermentation with Lactobacillus. The cooked sweet potato was fermented according to the factorial design with the following factors: the time (21.51h-38.48h) and the amount of Lactobacillus (1.58*106-5*106UFC). Responses were represented by production yield, pH, and titrable acidity. Characterization of the exopolysaccharides was then done by determination of total sugars, solubility index and viscosity. The results show that the pH of unfermented cooked potatoes was between 6.23 to 6.63. The pH of fermented potatoes varies from 3.96 to 4.06. The lactic acid content was found from 7.75 to 9.9% for the fermented samples. The production yields are 1.90% for the samples fermented for 21.5 hours with 3.5*106UFC of bacteria and 5.62% for those fermented for 30 hours with the same volume of inoculum. The average viscosity of the products was 4mPas regardless of the fermentation time. Chemical characterization indicates glucose contents of 68.21 and 94.01% in fermented potato for 24h and 21h respectively. The solubility index gives values of 70.3 ± 0.16 and 88.11 ± 0.23 for fermentation times of 21h and 24h respectively. Results of this work indicated that cooked sweet potatoes ferment for 21h was a promising substrate for production of exopolysaccharides.
Sweet potato (Ipomoea batatas L.) belongs to the Convolvulaceae family. It is the seventh major worldwide food crop after wheat, rice, corn, apple land, barley and cassava [1].It is the second foodstuff after cassava in tropical countries [2] and the third in Sub-Saharan Africa after cassava and yam [3] Cameroon had a production of 348,618 tonnes in 2013 and this production increases over time [4].
After cooking, sweet potato is the subject of losses, which could be an alternative to produce exopolysaccharides useful in food industries, health and agronomy. Indeed, when the cooked potato is left at room temperature for 24 hours, it gets slimy on the surface layer, indicating the presence of exopolysaccharides produced by bacterial during ambient fermentation. Exopolysaccharides (EPS) are also called capsular polysaccharides (CPS), or exo cellular polysaccharides [5].
They are of great interest in health, agro-food, and cosmetics. In food, they can improve the rheological and sensory properties of fermented products such as cheese and yogurt [6]. In bread making, they improve the viscoelastic properties and volume of the dough, and increase the self-live of bread, while reducing the hardness of the bread crust [7-8].
Most exopolysaccharides used in the food industry are obtained from plant substrates. Starch and its derivatives (gums, corn starch, tapioca, and cellulose) are the most used polymers to produce exopolysaccharides [9]. Exopolysaccharides can be produced from Tibetkefir with the use of Lactobacillus kefiranofaciens [10]. Lactic acid bacteria during growing have the capacity to generate exopolysaccharides. However, several factors can influence the production of exopolysaccharides such as: temperature, pH, number of microorganisms, time of fermentation, the fermentation substrate [11].
To bring added value to sweet potato cooked and to valorize the loses after cooking in Cameroon, the general objective of this work is to produce exopolysaccharides by lactic fermentation using the loses sweet potato after cooking. Specifically, the cooked sweet potatoes were ferment as a function of time and ratio microorganisms. The characterization of the exopolysaccharides was also performed.
2.1. Microorganisms and root tubers
The root tuber used in this work is sweet potato (Ipomoea batatas) the yellow variety which was bought at the Mokolo market inYaoundé in the Centre Region of Cameroon in July 2019. The Lactobacillus plantarum was kindly supplied by Professor Sado (Department of microbiology, University of Yaounde I, Cameroon). The microorganism was maintained at 4 °C in lactose (1g milk+20g of 1L of water and 1mL of Tween 80) and subcultured every 2 weeks. Figure 1 shows the general diagram of the work carried out.
2.2. Transplanting the strains
Transplanting of the strains (figure 2) was done to readjust the microorganisms to their environment. The microorganisms stored at -18 °C are stressed. They were put in optimal conditions so to renew young cells.
2.3. Production of one liter biomass
Biomass production was carried out to obtain a number of bacteria important for analyzes. It was done by the culture of bacteria in a nutritious medium at 37 ° C for 24h as shown on figure 3.
2.4. Determination of the microbial load of the obtained biomass.
The microbial load is determined in order to ferment the cooked sweet potato roots with fixed charges. For this, the biomass previously obtained is centrifuged at 4000 rpm for 30 min. The pellet is suspended in physiological water (1.7 g / 200 ml) and transferred to a sterile conical flask 0.1 ml of this mixture is sown in Petri dishes containing the medium culture Plate Count Agar (PCA: 6.9g / 300ml) previously sterilized and cooled. The whole was incubated at 37 °C. The counting is carried out after 24 hours.
2.5. Fermentation of cooked sweet potato roots.
The fermentation was conducted in 250 mL Erlenmeyer flask using 50 mL modified EPS production medium. The inoculated flask was incubated at 37 °C for different times (21h-38h) of fermentation under static condition. This fermentation process is illustrated in figure 4.
2.6. Extraction of exopolysaccharides.
Exopolysaccharides were extracted according to the method of [12] after boiling the culture at 100°C for 10 min to inactivate the EPS-degrading enzymes. The extraction of exopolysaccharides was done as shown in figure 5.
2.7. Characterization of exopolysaccharides.
To characterize the obtained exopolysaccharides, the analyses carried out were: titrable acidity, viscosity measurement, the solubility index, determination of total sugars. The yield was also calculated.
2.7. 1.Titrable acidity.
The titrable acidity of the fermented sweet potato was performed as follow: In 10g of fermented potatoe, a few drops of phenolphthalein were added. Then the mixture was titrated with 0.1N NaOH until the appearance of a pink color.
2.7. 2.Viscosity
The viscosity of exopolysaccharides was measured using a viscometer (Brookfield). Solutions (10% W/V) of exopolysaccharides are prepared. The mixture was stirred. The rotational viscometer was used to measures the viscosity. The stem is driven in rotation by a motor passing through a calibrated spring. Resistance to the flow increases as a function of the size of the rod and or the speed of rotation. Viscosity was expressed in mPas.
2.7. 3.Solubility index.
Solubility in water was performed according to the procedure of [13]. 10% exopolysaccharide (W / V) solutions were prepared. The mixture is stirred for 30 min then centrifuged at 3500 rpm for 15min. The solubility index is the percentage of exopolysaccharides dissolved in water after determination of the dry matter of the supernatant (24 h at 105° C). The solubility index is given by the equation:
Solubility index (%)=(Total carbohydrate concentration in supernatant )/(Weight of sample (dry weight basis))
2.7.4. Total sugars.
Total sugars in exopolysaccharides, was quantified using reducing sugar assay described by [14]. The results were expressed as glucose equivalent.
2.8. Statistical analyses.
A fractional factorial design, Box-Behnken model, was employed for the statistical optimization of the production conditions. The experimental design consisted of thirteen runs and the independent variables were studied at two different levels, a high level and a low level. The high level is commonly coded as +1 and the low level as -1. It is necessary to include center points as well (in which all factors are at their central values).
Two factors selected for optimization were the time of fermentation and the amount of microorganisms. A design was generated with these factors, having a low level and a high level. The low level for time of fermentation was 21h and the high level was 38h. The low level for amount of microorganism was 1.38*106 ml with a high level of 5*106 ml. The Minitab 18Software (v. 6.0, Stat-Ease, Inc., Minneapolis, USA) was used for experimental design. The pH, the titrable acidity of the media and the yield value of the produced EPS was taken as the response.
The statistical analysis of the model was performed in the form of analysis of variance (ANOVA). This analysis included the Fisher’s F-test (overall model significance), its associated probability p(F), correlation coefficient R, and determination coefficient R2 which measures the goodness of fit of the regression model. It also includes the Student’s t-value for the estimated coefficients and associated probabilities p(t). The quadratic models were represented as response surface graphs. Validation of the experiment was also performed by selecting different combinations of the factors as recommended by the software. All the experiments were performed in triplicates.
3.1. Microbial load of the biomass obtained
The microbial load required for the fermentation of sweet potato roots was 10-6. Dilutions were done to obtain these microbial loads. After 24h of incubation at 37°C, the colonies for each petri dish were counted. Several dilutions were carried out to obtain the microbial load. These results are presented in table 1. The dilutions used in this work were those between 10-6 and 10-8. In these cases, the dilution of 10-6 was used.
3.2. Yield of exopolysaccharide
The low yields of exopolysaccharide production by most Lactobacillus species are the main reason of their noncommercial exploitation. Therefore, among factors which affect the yield production of exopolysaccharides are time of fermentation, amount of microorganisms, the fermentation substrate, the temperature of fermentation, the pH of the media and the production of lactic acid [15-16]. In this study, the fermentation time and the amount of microorganism does not affect the yield of exopolysaccharides (table 2). The quadratic effect of the quantity of microorganisms and the time*quantity (p=0.91) interaction does not have effect on the yield (table 2).
The yield of cooked and fermented potato exopolysaccharides varies as a function of time and the amount of Lactobacillus (figure 6). At the minimum amount of 1.58*106 UFC, the production was 3.268g/100g for 30h. When the amount of microorganisms was increased up to 3.5*106 UFC, the yield was 5.682g/100g. Between 3.5*106 and 5.62*106 UFC a slight decrease of the production of exopolysaccharides is observed with yields of 5.682g/100g to 4.518g/100g respectively.
The production of exopolysaccharides increases with the time of fermentation. After 21.51h, the production of exopolysaccharides was 1.9g/100g. While after 38h 48min (the longest fermentation time), the production yield of exopolysaccharides was 4.321g/100 g. The maximum yield was obtained after 30 hours of fermentation and the corresponding value was 5.6g/100g. The time and quantity of microorganisms at which a maximun yield is obtained are 30h and 3.5ml of Lactobacillus respectively. Sethuraman et al. [17] had 9.3 g/l of exopolysaccharides after 100 hours of fermentation. They used the cooked sweet potato water as a growing medium. However, Yuliani et al. [18] found 3.8 g/l of exopolysaccharides after 48h 8min of fermentation. The yields observed by Sethuraman et al. [17] and Yuliani et al. [18] are lower than yields obtained with cooked sweet potato water. This can be explained by the fact that, after a long fermentation time, the amount of lactic acid in the medium was high. In fact, lactic acid stresses bacteria.
3.3. pH of the media
It has been shown that the pH of the media have a great impact on the production of exopolysaccharides. The best pH to produce exopolysaccharides varies around pH 5 and 6 [19]. The pH was measured before and after fermentation. The pH of the various treatments before fermentation is between 6.2 and 6.6. There is no significant difference between these values.
The effect of fermentation time and the amount of Lactobacillus are presented in table 3. The P value (0.95) of time and the quantity of microorganisms show that these two factors have no effect on the pH. Quadratic effect of quantity and interaction does not affect the pH. Yuliani et al. [18] extracted the exopolysaccharides from the water distilled soschu made from sweet potato and obtained maximum yield at pH 6.2. Sivakumar et al. [20] obtained exopolysaccharides from Frateuria aurentia by varying the pH from 3 to 9, after 72h of fermentation at 30 ° C. The pH of 6 and 7 were those at which the yield is maximum. This shows that the high yield depends on the experiment. The study of these authors shows that, to have an optimal production exopolysaccharide, the pH should be between 6 and 7. For this work, at the start of fermentation the pH values were between 6.2 and 6.6. At the end of fermentation the pH value range between 3.9 and 4.6 as shown at Figure 7. The drop of pH in the medium was due to the production of lactic acid and hydrogen by bacteria. That is why the next result was to verify the amount of titrable acidity in the medium.
3.4. Titrable acidity
Lactic acid bacteria as dominant organism in food fermentations will convert free sugars to lactic acid. The occurrence of lactic acid is based on the production of amylolytic enzymes by the bacteria that degrade starch granules and hydrolyze amylose and amylopectin [21].This enzyme production depends on the time of fermentation and the amount of microorganism in the media. Table 4 presents the analysis of variance of the effect of the fermentation time and the amount of lactic acid bacteria on the titrable acidity. The P-value (P≥0.05) of time and quantity shows that these two factors have no significant influence on titrable acidity. The effect of the time and the quantity of microorganisms does not also affect the titrable acidity at the secondary level and in interaction.
The variation of titrable acidity of the fermented cooked sweet potato is shown on figure 8. At a minimum amount of Lactobacillus of1.58 * 106 CFU, there was a production of lactic acid at 9.2%. When the amount of microorganism increased to 3.5 * 106 UFC, the titrable acidity was 9.8%. From 3.5 * 106 UFC to 5.62 * 106 UFC the production of titrable acidity was observed in the environment with values drops from 9.8% to 7.75% respectively. After 21h51min of fermentation, the titrable acidity produced, was at 8.66%. At 38h48min, the value rate increases to 9.9%. Bergmaier et al. [22] obtained 0.90 g of lactic acid per g of lactose consumed in the environment during the production of exopolysaccharides from potatoes by Lactobacillus rhamnosus. These authors observed that the increase of the fermentation time is related to that of lactic acid in the medium. After a large production of lactic acid in the medium, the bacteria are stressed and they no longer optimally produce exopolysaccharides. When there is a large amount of lactic acid in the medium, the pH drops and becomes acidic. It will therefore be necessary to adjust the pH to promote the production of exopolysaccharides.
Figure 9 shows the optimal area for the production of exopolysaccharides as a function of the fermentation time and the amount of microorganisms. The areas marked in white represent these optimal areas which are for the time being intervals ranging from 22h to 24h and 31h to 33h. The quantity of Lactobacillus used in this study has no influence on the production. Figure 9 confirms also that the lactic acid concentration and the pH are the parameters that affect the production of exopolysaccharides. This is why, Bergmaier et al. [22] found that a very high pH or a very low pH does not favor a high yield of exopolysaccharides production. In addition, the presence of a high amount of lactic acid in the environment stresses the bacteria and slows down their growth.
3.5. Physico-chemical characterization of exopolysaccharides.
In order to characterize the physico-chemical properties of the produced exopolysaccharides, the samples was analyses at the center and at the ends of the experimental design for their total sugars contents, solubility index and their viscosity.
3.5.1. Total sugars.
Exopolysaccharides are manly composed of sugars. Glucans are composed of glucose polymer backbone with various degrees of branching depending on producing strains. Exopolysaccharides were analysed for their sugar contents in term of glucose. Table 4 shows the amounts of sugar (glucose) in g present in 100gof exopolysaccharides from some samples of exopolysaccharides with respect to samples fermented for 21h, 24h, 30h and 38h. The glucose contents in the exopolysaccharide analysed samples vary from 68.21 ± 0.32g / 100g of exopolysaccharides and 94.01 ± 0.52g / 100g. These results show that glucose is the major sugar in these exopolysaccharides produced from sweet potatoes roots. Thus exopolysaccharides obtained would be glucans which are glucose polymers. They are composed of the monosaccharide monomer D-glucose, linked together by glycosidic bonds. Glucans can be divided into α-glucan and β-glucan.
3.6. Solubility index
Some glucans are both soluble and insoluble. That is why it was important to evaluate the solubility index of the exopolysaccharides. Solubility index is the maximum amount of substance that can dissolve in a certain volume of water. The solubility indices vary from 70.3 ± 0.16 to 88.11 ± 0.23%. These result shows that the exopolysaccharides obtained are very soluble in water. Zaheer et al. [10] extracted the exopolysaccharides from Tibet kefir. These authors obtained the solubility of 14.2%.The difference in solubility can be explained by the difference in the substrates. In addition to the substrate, the composition of the exopolysaccharides could bedifferent. In this investigation, after characterization, the exopolysaccharides contain more than 80% ofglucose. Glucose being a sugar very soluble in water, the solubility of exopolysaccharideswas very high.
3.7. Viscosity
The exopolysaccharides are visually slimy when they are produced. So, it is important to know the viscosity of the produced exopolysaccharide. The viscosity of the samples measured are all equal to 4mPas. Kanmani et al. [23] had viscosities equal to 208mPa at pH 6 and 226mPa at pH 3. Zaheer et al. [10] have obtained viscosity equal to 3.5mPa with a concentration of exopolysaccharides of2mg / ml and 4.5mPa with a concentration of exopolysaccharides of 4mg / ml. Kanmani et al. [23] observed that the viscosity of exopolysaccharides increases inacidic media. Zaheer et al. [10] demonstrate that the more the concentration increases, the more the viscosity increases. According to the concentrations (100mg/ml) used in this study, the viscosities (4mPas) obtained are lower than those noted by Zaheer et al. [10]. This can be explained by the fact that the viscosity values could vary with respect to the subtrates and the composition of exopolysaccharides.
This study opens new horizon for news sources of exopolysaccharides from local products. Loss cooked sweet potato root can be ferment and used to produce exopolysaccharides. These exopolysaccharides can be used in different field of food industry.
Dr. Panyoo acknowledges the Food and Nutrition Research Centre of the Institute of Medical Research and Medicinal Plant Studies of Cameroon for the laboratory’s facilities.
The authors do not have any conflict of interest.
Clearly Auctoresonline and particularly Psychology and Mental Health Care Journal is dedicated to improving health care services for individuals and populations. The editorial boards' ability to efficiently recognize and share the global importance of health literacy with a variety of stakeholders. Auctoresonline publishing platform can be used to facilitate of optimal client-based services and should be added to health care professionals' repertoire of evidence-based health care resources.
Journal of Clinical Cardiology and Cardiovascular Intervention The submission and review process was adequate. However I think that the publication total value should have been enlightened in early fases. Thank you for all.
Journal of Women Health Care and Issues By the present mail, I want to say thank to you and tour colleagues for facilitating my published article. Specially thank you for the peer review process, support from the editorial office. I appreciate positively the quality of your journal.
Journal of Clinical Research and Reports I would be very delighted to submit my testimonial regarding the reviewer board and the editorial office. The reviewer board were accurate and helpful regarding any modifications for my manuscript. And the editorial office were very helpful and supportive in contacting and monitoring with any update and offering help. It was my pleasure to contribute with your promising Journal and I am looking forward for more collaboration.
We would like to thank the Journal of Thoracic Disease and Cardiothoracic Surgery because of the services they provided us for our articles. The peer-review process was done in a very excellent time manner, and the opinions of the reviewers helped us to improve our manuscript further. The editorial office had an outstanding correspondence with us and guided us in many ways. During a hard time of the pandemic that is affecting every one of us tremendously, the editorial office helped us make everything easier for publishing scientific work. Hope for a more scientific relationship with your Journal.
The peer-review process which consisted high quality queries on the paper. I did answer six reviewers’ questions and comments before the paper was accepted. The support from the editorial office is excellent.
Journal of Neuroscience and Neurological Surgery. I had the experience of publishing a research article recently. The whole process was simple from submission to publication. The reviewers made specific and valuable recommendations and corrections that improved the quality of my publication. I strongly recommend this Journal.
Dr. Katarzyna Byczkowska My testimonial covering: "The peer review process is quick and effective. The support from the editorial office is very professional and friendly. Quality of the Clinical Cardiology and Cardiovascular Interventions is scientific and publishes ground-breaking research on cardiology that is useful for other professionals in the field.
Thank you most sincerely, with regard to the support you have given in relation to the reviewing process and the processing of my article entitled "Large Cell Neuroendocrine Carcinoma of The Prostate Gland: A Review and Update" for publication in your esteemed Journal, Journal of Cancer Research and Cellular Therapeutics". The editorial team has been very supportive.
Testimony of Journal of Clinical Otorhinolaryngology: work with your Reviews has been a educational and constructive experience. The editorial office were very helpful and supportive. It was a pleasure to contribute to your Journal.
Dr. Bernard Terkimbi Utoo, I am happy to publish my scientific work in Journal of Women Health Care and Issues (JWHCI). The manuscript submission was seamless and peer review process was top notch. I was amazed that 4 reviewers worked on the manuscript which made it a highly technical, standard and excellent quality paper. I appreciate the format and consideration for the APC as well as the speed of publication. It is my pleasure to continue with this scientific relationship with the esteem JWHCI.
This is an acknowledgment for peer reviewers, editorial board of Journal of Clinical Research and Reports. They show a lot of consideration for us as publishers for our research article “Evaluation of the different factors associated with side effects of COVID-19 vaccination on medical students, Mutah university, Al-Karak, Jordan”, in a very professional and easy way. This journal is one of outstanding medical journal.
Dear Hao Jiang, to Journal of Nutrition and Food Processing We greatly appreciate the efficient, professional and rapid processing of our paper by your team. If there is anything else we should do, please do not hesitate to let us know. On behalf of my co-authors, we would like to express our great appreciation to editor and reviewers.
As an author who has recently published in the journal "Brain and Neurological Disorders". I am delighted to provide a testimonial on the peer review process, editorial office support, and the overall quality of the journal. The peer review process at Brain and Neurological Disorders is rigorous and meticulous, ensuring that only high-quality, evidence-based research is published. The reviewers are experts in their fields, and their comments and suggestions were constructive and helped improve the quality of my manuscript. The review process was timely and efficient, with clear communication from the editorial office at each stage. The support from the editorial office was exceptional throughout the entire process. The editorial staff was responsive, professional, and always willing to help. They provided valuable guidance on formatting, structure, and ethical considerations, making the submission process seamless. Moreover, they kept me informed about the status of my manuscript and provided timely updates, which made the process less stressful. The journal Brain and Neurological Disorders is of the highest quality, with a strong focus on publishing cutting-edge research in the field of neurology. The articles published in this journal are well-researched, rigorously peer-reviewed, and written by experts in the field. The journal maintains high standards, ensuring that readers are provided with the most up-to-date and reliable information on brain and neurological disorders. In conclusion, I had a wonderful experience publishing in Brain and Neurological Disorders. The peer review process was thorough, the editorial office provided exceptional support, and the journal's quality is second to none. I would highly recommend this journal to any researcher working in the field of neurology and brain disorders.
Dear Agrippa Hilda, Journal of Neuroscience and Neurological Surgery, Editorial Coordinator, I trust this message finds you well. I want to extend my appreciation for considering my article for publication in your esteemed journal. I am pleased to provide a testimonial regarding the peer review process and the support received from your editorial office. The peer review process for my paper was carried out in a highly professional and thorough manner. The feedback and comments provided by the authors were constructive and very useful in improving the quality of the manuscript. This rigorous assessment process undoubtedly contributes to the high standards maintained by your journal.
International Journal of Clinical Case Reports and Reviews. I strongly recommend to consider submitting your work to this high-quality journal. The support and availability of the Editorial staff is outstanding and the review process was both efficient and rigorous.
Thank you very much for publishing my Research Article titled “Comparing Treatment Outcome Of Allergic Rhinitis Patients After Using Fluticasone Nasal Spray And Nasal Douching" in the Journal of Clinical Otorhinolaryngology. As Medical Professionals we are immensely benefited from study of various informative Articles and Papers published in this high quality Journal. I look forward to enriching my knowledge by regular study of the Journal and contribute my future work in the field of ENT through the Journal for use by the medical fraternity. The support from the Editorial office was excellent and very prompt. I also welcome the comments received from the readers of my Research Article.
Dear Erica Kelsey, Editorial Coordinator of Cancer Research and Cellular Therapeutics Our team is very satisfied with the processing of our paper by your journal. That was fast, efficient, rigorous, but without unnecessary complications. We appreciated the very short time between the submission of the paper and its publication on line on your site.
I am very glad to say that the peer review process is very successful and fast and support from the Editorial Office. Therefore, I would like to continue our scientific relationship for a long time. And I especially thank you for your kindly attention towards my article. Have a good day!
"We recently published an article entitled “Influence of beta-Cyclodextrins upon the Degradation of Carbofuran Derivatives under Alkaline Conditions" in the Journal of “Pesticides and Biofertilizers” to show that the cyclodextrins protect the carbamates increasing their half-life time in the presence of basic conditions This will be very helpful to understand carbofuran behaviour in the analytical, agro-environmental and food areas. We greatly appreciated the interaction with the editor and the editorial team; we were particularly well accompanied during the course of the revision process, since all various steps towards publication were short and without delay".
I would like to express my gratitude towards you process of article review and submission. I found this to be very fair and expedient. Your follow up has been excellent. I have many publications in national and international journal and your process has been one of the best so far. Keep up the great work.
We are grateful for this opportunity to provide a glowing recommendation to the Journal of Psychiatry and Psychotherapy. We found that the editorial team were very supportive, helpful, kept us abreast of timelines and over all very professional in nature. The peer review process was rigorous, efficient and constructive that really enhanced our article submission. The experience with this journal remains one of our best ever and we look forward to providing future submissions in the near future.
I am very pleased to serve as EBM of the journal, I hope many years of my experience in stem cells can help the journal from one way or another. As we know, stem cells hold great potential for regenerative medicine, which are mostly used to promote the repair response of diseased, dysfunctional or injured tissue using stem cells or their derivatives. I think Stem Cell Research and Therapeutics International is a great platform to publish and share the understanding towards the biology and translational or clinical application of stem cells.
I would like to give my testimony in the support I have got by the peer review process and to support the editorial office where they were of asset to support young author like me to be encouraged to publish their work in your respected journal and globalize and share knowledge across the globe. I really give my great gratitude to your journal and the peer review including the editorial office.
I am delighted to publish our manuscript entitled "A Perspective on Cocaine Induced Stroke - Its Mechanisms and Management" in the Journal of Neuroscience and Neurological Surgery. The peer review process, support from the editorial office, and quality of the journal are excellent. The manuscripts published are of high quality and of excellent scientific value. I recommend this journal very much to colleagues.
Dr.Tania Muñoz, My experience as researcher and author of a review article in The Journal Clinical Cardiology and Interventions has been very enriching and stimulating. The editorial team is excellent, performs its work with absolute responsibility and delivery. They are proactive, dynamic and receptive to all proposals. Supporting at all times the vast universe of authors who choose them as an option for publication. The team of review specialists, members of the editorial board, are brilliant professionals, with remarkable performance in medical research and scientific methodology. Together they form a frontline team that consolidates the JCCI as a magnificent option for the publication and review of high-level medical articles and broad collective interest. I am honored to be able to share my review article and open to receive all your comments.
“The peer review process of JPMHC is quick and effective. Authors are benefited by good and professional reviewers with huge experience in the field of psychology and mental health. The support from the editorial office is very professional. People to contact to are friendly and happy to help and assist any query authors might have. Quality of the Journal is scientific and publishes ground-breaking research on mental health that is useful for other professionals in the field”.
Dear editorial department: On behalf of our team, I hereby certify the reliability and superiority of the International Journal of Clinical Case Reports and Reviews in the peer review process, editorial support, and journal quality. Firstly, the peer review process of the International Journal of Clinical Case Reports and Reviews is rigorous, fair, transparent, fast, and of high quality. The editorial department invites experts from relevant fields as anonymous reviewers to review all submitted manuscripts. These experts have rich academic backgrounds and experience, and can accurately evaluate the academic quality, originality, and suitability of manuscripts. The editorial department is committed to ensuring the rigor of the peer review process, while also making every effort to ensure a fast review cycle to meet the needs of authors and the academic community. Secondly, the editorial team of the International Journal of Clinical Case Reports and Reviews is composed of a group of senior scholars and professionals with rich experience and professional knowledge in related fields. The editorial department is committed to assisting authors in improving their manuscripts, ensuring their academic accuracy, clarity, and completeness. Editors actively collaborate with authors, providing useful suggestions and feedback to promote the improvement and development of the manuscript. We believe that the support of the editorial department is one of the key factors in ensuring the quality of the journal. Finally, the International Journal of Clinical Case Reports and Reviews is renowned for its high- quality articles and strict academic standards. The editorial department is committed to publishing innovative and academically valuable research results to promote the development and progress of related fields. The International Journal of Clinical Case Reports and Reviews is reasonably priced and ensures excellent service and quality ratio, allowing authors to obtain high-level academic publishing opportunities in an affordable manner. I hereby solemnly declare that the International Journal of Clinical Case Reports and Reviews has a high level of credibility and superiority in terms of peer review process, editorial support, reasonable fees, and journal quality. Sincerely, Rui Tao.
Clinical Cardiology and Cardiovascular Interventions I testity the covering of the peer review process, support from the editorial office, and quality of the journal.
Clinical Cardiology and Cardiovascular Interventions, we deeply appreciate the interest shown in our work and its publication. It has been a true pleasure to collaborate with you. The peer review process, as well as the support provided by the editorial office, have been exceptional, and the quality of the journal is very high, which was a determining factor in our decision to publish with you.
The peer reviewers process is quick and effective, the supports from editorial office is excellent, the quality of journal is high. I would like to collabroate with Internatioanl journal of Clinical Case Reports and Reviews journal clinically in the future time.
Clinical Cardiology and Cardiovascular Interventions, I would like to express my sincerest gratitude for the trust placed in our team for the publication in your journal. It has been a true pleasure to collaborate with you on this project. I am pleased to inform you that both the peer review process and the attention from the editorial coordination have been excellent. Your team has worked with dedication and professionalism to ensure that your publication meets the highest standards of quality. We are confident that this collaboration will result in mutual success, and we are eager to see the fruits of this shared effort.
Dear Dr. Jessica Magne, Editorial Coordinator 0f Clinical Cardiology and Cardiovascular Interventions, I hope this message finds you well. I want to express my utmost gratitude for your excellent work and for the dedication and speed in the publication process of my article titled "Navigating Innovation: Qualitative Insights on Using Technology for Health Education in Acute Coronary Syndrome Patients." I am very satisfied with the peer review process, the support from the editorial office, and the quality of the journal. I hope we can maintain our scientific relationship in the long term.
Dear Monica Gissare, - Editorial Coordinator of Nutrition and Food Processing. ¨My testimony with you is truly professional, with a positive response regarding the follow-up of the article and its review, you took into account my qualities and the importance of the topic¨.
Dear Dr. Jessica Magne, Editorial Coordinator 0f Clinical Cardiology and Cardiovascular Interventions, The review process for the article “The Handling of Anti-aggregants and Anticoagulants in the Oncologic Heart Patient Submitted to Surgery” was extremely rigorous and detailed. From the initial submission to the final acceptance, the editorial team at the “Journal of Clinical Cardiology and Cardiovascular Interventions” demonstrated a high level of professionalism and dedication. The reviewers provided constructive and detailed feedback, which was essential for improving the quality of our work. Communication was always clear and efficient, ensuring that all our questions were promptly addressed. The quality of the “Journal of Clinical Cardiology and Cardiovascular Interventions” is undeniable. It is a peer-reviewed, open-access publication dedicated exclusively to disseminating high-quality research in the field of clinical cardiology and cardiovascular interventions. The journal's impact factor is currently under evaluation, and it is indexed in reputable databases, which further reinforces its credibility and relevance in the scientific field. I highly recommend this journal to researchers looking for a reputable platform to publish their studies.
Dear Editorial Coordinator of the Journal of Nutrition and Food Processing! "I would like to thank the Journal of Nutrition and Food Processing for including and publishing my article. The peer review process was very quick, movement and precise. The Editorial Board has done an extremely conscientious job with much help, valuable comments and advices. I find the journal very valuable from a professional point of view, thank you very much for allowing me to be part of it and I would like to participate in the future!”
Dealing with The Journal of Neurology and Neurological Surgery was very smooth and comprehensive. The office staff took time to address my needs and the response from editors and the office was prompt and fair. I certainly hope to publish with this journal again.Their professionalism is apparent and more than satisfactory. Susan Weiner
