info@auctorespublishing.com
+1-(302)-520-2644
+1-(302)-520-2644

Preclinical Study to Evaluate the Effects of a Soft Handkerchief in Nasolabial Skin Barrier

  1. Home
  2. Journals
  3. Dermatology and Dermatitis
  4. Archives
Submit Guidelines

Research

Preclinical Study to Evaluate the Effects of a Soft Handkerchief in Nasolabial Skin Barrier

  • Samara Eberlin 1*
  • Liliana Torloni 2
  • Angélica Richart Csipak 2
  • Gustavo Facchini 1
  • Gustavo Henrique da Silva 1
  • Camila Martins Kawakami 1
  • Ana Lúcia Tabarini Alves Pinheiro 1
  • Vivienne Carduz Castilho 2

*Corresponding Author: Samara Eberlin, Kosmoscience Group, Rua Sandoval Meirelles, 72 – Vila João Jorge, CEP: 13040-315 - Campinas/SP, Brazil.

Citation: S Eberlin, L Torloni, A RCsipak, G Facchini, G H da Silvat et al. (2021) Preclinical study to evaluate the effects of a soft handkerchief in nasolabial skin barrier. J. Dermatology and Dermatitis. 6(2); Doi:10.31579/2578-8949/078

Copyright: ©2021 Samara Eberlin, This is an open-access article distributed under the terms of The Creative Commons. Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Received: 03 June 2021 | Accepted: 08 July 2021 | Published: 16 July 2021

Keywords: skin barrier; fillagrin, histamina; sensitive skin; wipes; skin care; ex vivo; skin; microbiota

Abstract

People suffering from an ordinary acute cold consume so many handkerchiefs that the wiping actions on their own increase the abrasive damage of the nasolabial zone, finally leading to a disturbed barrier function and inflamation. It seems that the quality of the material used for nose cleansing could play an important role and that innovative handkerchiefs would fulfil a preventive role in minimizing the damaging effect of theskin barrier function of the nasolabial zone during this conditions. The objective of this work was to evaluate the effects of a wet handkerchief (SKNW) on the skin barrier balance by measuring filaggrin and histamine using an experimental model of ex vivo native human skin model and the interference of this handkerchief in the skin microbiota through in vitro screening. SKNW showed an increase in the production of filaggrin and a reduction of histamine synthesis in human explants subjected to barrier disruption with 5% Sodium Lauryl Sulfate. Additionaly, SKNW showed a mild and moderate antiseptic action on the evaluated microorganisms. This study demonstrated that SKNW could be considered a feasible option for consecutive wiping of nasolabial zone avoiding the transient mechanical dermatitis, considering its skin barrier protective, non-irritating and antiseptic actions.

Abbreviations

SC – Stratum corneum

SKNW – SalsepÒ Kids Nasal Wipes 

IL-1a- interleukin-1 alpha

IL-1β- interleukin-1 beta 

TNFa-Tumor necrosis fator a

NMFs – Natural moiusturizing factors

 BHI – Brain Heart Infusion Broth

CFU – Colony forming units

Introduction

The occurrence of runny nose due to rhinitis, flu and colds is very common in infants. Published studies carried out in Brazilian children have shown that about 48%had at least one episode of sneezing, runny nose or stuffy nose without a cold or flu in the first year of life, and that the average prevalence of rhinitis in children was 26.6% [1,2].

Under this clinical situation, frequent rubbing of the nasolabial skin with handkerchiefs can provoke skin irritation, which – at first – heals difficultly because the skin reacts by an inflammatory response leading to hyperkeratotic scaly areas. These clinical symptoms of a mechanically induced dermatitis probably are caused by removal of the superficial hydrolipidic layer and disturbance of the barrier function in the upper part of the Stratum corneum (SC) [3].

The natural barrier of SC depends on its composition, formed mostly by highly insoluble and resistant proteins, which involve externally keratinocytes being fundamental in the structure and organization of intracellular lipids [4]. Profilaggrin, filaggrin, involucrin and loricin are examples of these proteins which are responsible for the association of intermediate keratin filaments and consequent increase of cohesion among corneocytes [4].

Insult of skin barrier promotes immediately release of pro-inflammatory cytokines, along chemokines and growth factors, which will stimulate the migration and proliferation of immunocompetent cells [5]. Inflammatory signaling via nuclear transcription factor kappa B (NF-kB) is also activated and results in the production of more cytokines and chemokines, as well as, trigger the classic pathway of inflammation and increasing the production of eicosanoids, such as prostaglandins and leukotrienes [6,7]. Furthermore, a variety of mediators, such as, vascular endothelial growth factor, nitric oxide and histamine are produced leading to vasodilation, edema, mast cell degranulation, pain and itching, exacerbating the inflammatory process and skin sensivity [6, 8-9].

Concurrently, local microbiota can be significantly disturbed resulting in a cutaneousdysbiosis that unbalances the immune system and favors the growth of pathogenic microorganisms, predisposing to secondary infections [10-12].

It seems that the quality of the paper material used for nose cleansing could play an important role and that soft handkerchiefs would cause less damage on the hydrolipidic film and the skin barrier when having a cold. Soft paper handkerchiefs might then fulfil a preventive role in minimizing the damaging effect of the skin barrier function of the nasolabial zone [13].

The objective of this work was to evaluate the effects of a skin care handkerchiefs on the nasolabial skin barrier balance by measuring filaggrin and histamine using an experimental model of ex vivo native skin. Additionally, an in vitro screening was carried out with the purpose of evaluating the interference of these wipes in skin microbiota.

Materials and methods

Test substance

Test substance consists of a wet handkerchief – SalsepR Kids Nasal Wipes (SKNW) mainly indicated for cleaning nasal secretions, crusts and runny nose caused by flu, colds and rhinitis in infants. SKNW was provided by Libbs Farmacêutica Ltda, São Paulo/SP–Brazil (INCI names: Aqua, Myristamidopropyl PG-Dimonium Chloride Phosphate, Sodium Chloride, Tocopheryl Acetate, Aloe Barbadensis leaf juice, Glycerin, Chamomilla Recutita Flower Extract, Potassium sorbate, Decyl Glucoside, Citric acid, Sodium citrate, Sodium benzoate).

Skin fragments culture

Human skin fragments were originating from one (01) healthy donor, female, skin type III, 43 years, who underwent elective plastic surgery in the abdominal region (abdominoplasty). After the surgical procedure, the skin fragments were collected in plastic vials containing 0.9% saline and kept in refrigeration for up to 24 hours. The use of human skin fragments from elective surgeries for this study was approved by the Ethics Committee of the University São Francisco – SP.

Treatment protocols and stress conditions for skin culture

Skin fragments were fractionated into pieces of approximately 1.5 cm2 and incubated in culture medium (DMEM; Sigma-Aldrich, San Luis, MO, USA) in air-liquid interface at 37°C in a humidified atmosphere with 5% CO2. Fragments were treated with SKNW, simulating the condition of using a wet wipe for 3 consecutive days. On the third day, the fragments were subjected to barrier disruption with Sodium Lauryl Sulfate (5% - SLS; Sigma) and treated with SKNW. After 24 hours the third treatment, the skin culture supernatant was collected for measurement of histamine and the fragments subjected to histological processing for immunofluorescence and semi-quantification of filaggrin.

Quantification of histamine

Concentrations of histamine were measured by ELISA using commercially available kit (Uscn Life Sciences, Wuhan, China). Absorbance reading was performed on Multiskan GO monochromator (Thermo Fisher Scientific, Waltham, MA, USA).

Immunofluorescence evaluation for Filaggrin

Skin fragments were fixed in 4% paraformaldehyde (pH 7.4) for 24 hours and cryoprotected in a 30% sucrose solution for 72 hours. Fragments were embedded in a formulation of water-soluble glycols and resins (Tissue-Tek ® OCT ™, Torrance, CA, USA) and then 10 µm serial sections were collected directly on silanized slides with the aid of cryostat (Leica - CRYOCUT 1800, Germany). Slides were incubated overnight with primary antibody anti-FILAGGRIN (Abcam, Cambridge, UK). Subsequently, the cuts were washed and incubated with Alexa Fluor 488 secondary antibody - Goat anti Rabbit (Thermo Fisher Scientific). DAPI (4'-6-Diamidino-2-Phenylindol; DNA marker; Sigma-Aldrich) was used as a DNA marker. The slides were mounted in a specific mounting medium and analyzed using a Fluorescence Microscope (OLYMPUS) with the aid of the cellSens Standardsoftware (© 2010 OLYMPUS CORPORATION, Center Valley, PA, USA). The evaluated parameter was the fluorescence intensity emitted by the specific antibody labeling. After obtaining the images, the fluorescence intensity was quantified with the aid of the ImageJ software(Arbitrary Units - A.U.)

Statistical analysis

For statistical evaluation, ANOVA test was used to measure the variation of the results, comparing the data between all the groups. We applied the Bonferroni post-test, which strengthened and made the result presented in the ANOVA more precise (GraphPad Prism6, Version 6.01, GraphPad Software, Inc., La Jolla, CA, USA). A 5% significance level was used.

Microbiological inhibition

The antibacterial activity of SKNW was assessed through agar diffusion test against three bacteria species: Staphylococcus aureus ATCC 6538 (American Type Culture Collection, Rockville, MD), Staphylococcus epidermidis ATCC 12228 and Cutibacterium acnes ATCC 6919. The microorganisms were maintained in BHI for 24hat 35ºC and each bacterial suspension (inoculum) was diluted to 108 CFU/mL and uniformly spread on a sterile Petri dish containing Muller Hinton agar. SKNW was cut into 1cm2 and added to each of the 3 wells (8 mm diameter holes cut in the agar gel, 20 mm apart from one another). The systems were incubated for 48h and the inhibition of the bacterial growth around each well was measured in mm. The inhibition was classified as mild - halo up to 4 mm, moderate - halo from 5 mm to 9 mm, and strong halo above 10 mm [14].

Results and Discussion

In this work, we present a combination of preclinical results obtained for a wet handkerchief (SKNW) mainly indicated for cleaning nasal secretions, crusts and runny nose caused by flu, colds and rhinitis, considering the skin barrier protective, non-irritating and antiseptic actions. These characteristics are desired for this category of products, considering the need for repeated use for hygiene of the nasal region in situations of runny nose. The softness of material used for cleaning nasolabial area is determining to cause less damage on skin barrier during flu crisis [13].

A wide variety of methods can be employed to measure the in vitro activity of microorganisms against antimicrobial agents in products, which can be classified into three types: bioautographic, diffusion and dilution assays [15]. To evaluate the antiseptic action of the SKNW, we opted by agar diffusion method. Diffusion antibiogram testing is a conventional way to measure antimicrobial activity and is based on diffusion of the product or substance into a solid culture medium with the inoculated microorganism. From this diffusion, a halo appears around the product if it has antimicrobial properties [15-16]. The reading of the results is done after 48 hours, where it is checked the presence or absence of halo formation around the orifice where the sample is placed.

SKNW promoted a mild to moderate inhibition in the growth of Staphylococcus aureus, Staphylococcus epidermidis and Cutibacterium acnes, as described in Table 1. Specifically, the inhibition zone was 1.90 mm for Staphylococcus aureus (mild inhibition), 6.42 mm for Staphylococcus epidermidis (moderate inhibition) and 5.58 mm (moderate inhibition) for Cutibacterium acnes. Regarding the experimental controls, there was proper development of the microorganisms evaluated in the positive control plates and in the negative control there was no type of growth(Figure 1).

Bacteria evaluated in this study are part of the skin microbiota and play an important role in the maintenance of cutaneous metabolism. The balance between the populations of Staphylococcus aureus, Staphylococcus epidermidis and Cutibacterium acnes, can attribute beneficial properties to products applied topically, particularly in certain skin conditions, such as transient barrier disturbance, sensitive skin, dermatitis, and acne [17-18]. Clearly, this is only a preliminar result, however despitethe limitations of the present study, we can suggest that SKNW showed a mild and moderate antiseptic action on the evaluated microorganisms.

Table 1. Results and classification of inhibition halos obtained for each microorganism evaluated.
Figure 1. Inhibition halo formed after incubation of the microorganisms Staphylococcus aureus, Staphylococcus epidermidis and Cutibacterium acnes with SKNW and their respective control groups

Regarding the protective ability on skin barrier, we evaluated in this work the effects of SKNW on the production of envelope protein filaggrin using ex vivo human skin model submitted to epidermal barrier disruption [19-21]. Figures 2 and 3 represent the results of immunostaining and and subjected to barrier disruption with Sodium Lauryl Sulfate (5% - SLS).  As expected protein semi-quantification of filaggrin, respectively, in human skin culture treated with SKNW, barrier disruption induced with 5% SLS produced a 59.32% reduction in filaggrin synthesis when compared to baseline control (P<0>

These results allow us to infer that SKNW has a positive effect on the protection and maintenance of skin barrier by increasing the synthesis of filaggrin. The epidermal protein profilaggrin, lately synthesized during epidermal differentiation, plays an important role in generating and maintaining SC flexibility and moisturizing [22]. This highly phosphorylated protein is quickly dephosphorylated and proteolyzed during the end of the transition from granular keratinocyte to the corneocyte, where it remains retained. During the transition from the granular layer to SC, profilaggrin is converted to filaggrin by proteolysis and dephosphorylation [23-26]. In the SC, filaggrin is released from the interactions with keratin [27] and completly degraded into amino-acid constituents, which in turn, constitute approximately 50% of natural moiusturizing factors (NMFs). NMFs are crucial for the maintenance of the epidermal moisture barrier, and they are reduced in dry skin, particularly during aging and seasonal alterations [27].

Figure 2: Immunofluorescence evaluation of filaggrin protein labeling in human skin fragments incubated with the evaluated product SKNW and submitted to barrier disruption with Sodium Lauryl Sulfate (5%-SLS). Filaggrin is marked in green and the blue mark represents the cell nucleus(DNA). The reference bar corresponds to 20 μm.
Figure 3: Semi-qualification of the fluorescence intensity of filagrin protein immunostaining in fragments of human skin fragments incubated with the evaluated product SKNW and submitted to barrier disruption with Sodium Laury1 Sulfate (5% - SLS). The data represent the mean +- standard deviation of 09 areas (ANOVA – Bonferroni).

Another important aspect to be considered during mechanically induced skin irritation is the exacerbation of the inflammatory response that occurs due to the increased permeability of the skin barrier [28-30]. Several inflammatory mediators are locally produced stimulating skin mast cells activation and histamine release, which in turn, result in degranulation, growth and/or survival of skin mast cells through the high affinity to the neurokinin-1 (NK-1) receptor [31-33]. Histamine is one of the most important mediators released during the first stage of allergic and irritating reaction [31] and is involved with pruritus, erythema and scaliness [34]. In the skin, beyond the mast cells, keratinocytes also produce and release histamine in response to stimuli [35-36]. Histamine exerts its effects by binding to its receptors(H1R to H4R), present in keratinocytes (H1R,H2R and H4R) and Langerhans cells (H4R) [37], which activate the inflammatory signaling pathway NF Kappa B [38].

In this study, we evaluated the effects of the SKNW on the production of histamine in human skin cultures subjected to barrier disruption with 5 % SLS and the results are shown in Figure 4. As can be seen, the barrier disruption with SLS increased histamine production by 52.26times compared to baseline control(P<0>). The treatment with SKNW was able to significantly reduce the excessive production of this mediator by 60.02%(P <0>), when compared to the SLS group.

This result points to an antihistaminic action of the SKNW, which indirectly acts through anti-inflammatory mechanisms, such as inhibition of mast cell and basophil degranulation, inhibition of adhesion molecules and cell chemotaxis, enhancing apoptosis of inflammatory cells and reduction of cytokine/chemokine expression [39]. In addition to the effects on the inhibition of inflammatory and irritative response, the downregulation of histamine production promotes the recovery of skin barrier. Studies indicate that the excessive production of histamine suppressed epidermal differentiation by significant reductions in filaggrin, loricrin and keratin 10 expressions in keratinocytes culturesand epidermal human models, mediated by H1R activation on keratinocytes [40]. Besides, it has been known that histamine disrupts tight junctions, reduces transepithelial electric resistance (TEER) and enhances permeability in human skin explants [33].

Figure 4: Effects of evaluated product SKNW on histamine production in human skin fragments submitted to barrier disruption with Sodium Lauryl Sulfate (5% - SLS). The data represent the mean ± standard deviation of 03 replicates (ANOVA - Bonferroni).

The promising effects promoted by SKNW in this work can be attributed to its composition - Tocopheryl Acetate, Aloe barbadensis (leaf juice) and Chamomilla recutita (flower extract).

Tocopheryl acetate, also known as vitamin E acetate, is a synthetic form of vitamin E which is widely used in dermatological products mainly for its undeniable antioxidant action [41]. Vitamin E is part of the antioxidant defense system of the organism and has important photoprotective and anti-photoaging role in the skin [42]. In the epidermis, tocopheryl acetate inhibits the production of prostaglandin E2, nitric oxide and protects against lipid peroxidation and edema formation induced by UV radiation [43-45]. In patients with atopic dermatitis, daily supplementation of 400 IE of vitamin E promoted a reduction in IgE levels and the clinical manifestations of this disorder [45]. In vitro and clinical studies also indicate an anti-inflammatory action of alpha-tocopherol through inhibition of NFBand proinflammatory cytokines [47-49].

Aloe barbadensis Miller or Aloe vera Linne is a tropical succulent plant widely used from skin disorders. It can be effective for psoriasis, seborrheic dermatitis, aphthous stomatitis, xerosis, lichen planus, frostbite, genital herpes, human papilloma virus, burn, wound healing and inflammation [50-55].It can also be used for its antimicrobial and antifungal properties, in addition to photodynamic therapy of some cancers [50]. Maenthaisong et al. conducted a meta-analysis clinical study that showed the healing time for burn wound, for first to second degree, in Aloe vera treated-group was 8.79 days shorter than those in the control group [51]. Regarding skin effects, Aloe vera was described to stimulate collagen and hyaluronic acid production by human dermal fibroblastos [54]. Oral intake of Aloe vera contibutes to maintaining healthy skin, since skin elasticity, collagen score, skin moisturizing and transepidermal water loss were significantly improved after 12-week of use [54]. Likewise, hydration and skin barrier improvement of Aloe extract were confirmed after 2-weeks of topical application [56].

The mechanisms by which this plant exerts its effects are closely linked to its diverse composition of polysaccharides, glycoproteins, lectins, anthraquinone, and phenolic compounds, among others. Mucopolysaccharides exert protective effects on epithelial barriers, such as stomachs and duodenum, reducing the susceptibility to allergies and irritations [52]. Aloe also presents antioxidants, anti-inflammatory and strong immunomodulatory properties, activating macrophages which gives an antimicrobial action to this species [53].

Another ingredient presents in SKNW is the Chamomilla Recutita flower extract, defined by Srivastava et al. as “a herbal medicine of the past with bright future” [57]. In fact, chamomile is one of the oldest, most widely used, and well-documented medicinal plants in the world and has been recommended for a variety of healing applications [58]. Terpenoids and flavonoids are the main bioactive constituents of chamomile and present anti-inflammatory and antioxidants activities [59-60]. Chamomile is vastly used to treat inflammations of the skin and mucous membranes, such as diaper rash and cracked nipples, and for various bacterial infections of the skin, oral cavity and gums, and respiratory tract [61-62]. A clinical study demonstrated that chamomile flavonoids and essential oils penetrate skin surfaces into the deeper skin layers [63]. This fact supports their use as the topical antiphlogistic (anti-inflammatory) agent through mechanisms that involve inhibition of LPS-induced prostaglandin E2 release and attenuation of cyclooxygenase (COX-2)enzyme activity without affecting the constitutive form, COX-1 [65]. In this respect, topical applications of chamomile have been shown to be moderately effective in the treatment of atopic eczema [65]. It was found to be about 60% as effective as 0.25% hydrocortisone cream [66]. Compared to corticosteroids, chamomile was also suggesting to present faster and complete wound healing[67].

In summary, this preclinical study showed that the combination of specific ingredients in SKNW was able to present a considerable approach in the mechanisms involving in skin barrier integrity. This study demonstrated that SKNW could be considered a feasible option for consecutive wiping of the nasolabial zone avoiding the transient mechanical dermatitis, considering its skin barrier protective, non-irritating, and antiseptic actions.

Acknowledgements

This work was supported by grantsfrom Libbs Farmacêutica Ltda. The authors also express many thanks to Dr. Mark Little for their technical assistance.

References

  1. Chong Neto HJ, Bojarski LFM, Rosário CS, Rosário BA, Chong FH, Grasselli EA, et al (2014). Allergic rhinitis in preschool children from southern Brazil. Allergy 69: 545-547.
    View at Publisher | View at Google Scholar
  2. Solé D, Camelo-Nunes IC, Vana AT, Yamada E, Werneck F, Freitas LS, et al (2004). Prevalence of rhinitis and related-symptoms in schoolchildren from different cities in Brazil. Allergol et Immunopathol 32(1):7-12.
    View at Publisher | View at Google Scholar
  3. Houben E, Adam R, Hachem JP, Roseeuw D, Rogiers V, Paepe K (2008). Clinical scoring and biophysical evaluation of nasolabial skin barrier damage caused by rhinorrhea. Contact Dermatitis 59: 296-300.
    View at Publisher | View at Google Scholar
  4. Ishida-Yamamoto A, Iizuka H (1998). Structural organization of cornified cell envelopes and alterations in inherited skin disorders. Exp Dermatol 7: 1-10.
    View at Publisher | View at Google Scholar
  5. Williams IR, Kupper TS (1996). Immunity at the surface: homeostatic mechanisms of the skin immune system. Life Sci 58: 1485-1507.
    View at Publisher | View at Google Scholar
  6. Paus R, Theoharides TC, Arck PC (2006). Neuroimmunoendocrine circuitry of the „brain-skin connection‟. Trends Immunol 27: 32-39.
    View at Publisher | View at Google Scholar
  7. Luger TA (2002). Neuromediators – a crucial component of the skin immune system. J. Dermatol Sci 30: 87-93.
    View at Publisher | View at Google Scholar
  8. Grouzmann E, Bigliardi P, Appenzeller M, Pannatier A, Buclin T (2011). Substance P-induced skin inflammation is not modulated by a single dose of sitagliptin in human volunteers. Biol Chem 392: 217- 221.
    View at Publisher | View at Google Scholar
  9. Misery L, Myon E, Martin N, Consoli S, Bousetta S, Nocera T, et al (2007). Sensitive skin: psychological effects and seasonal changes. J Eur Acad Dermatol Venereol 21: 620-628.
    View at Publisher | View at Google Scholar
  10. Baldwin HE, Bhatia ND, Friedman A, Eng RM, Seite S (2017). The Role of Cutaneous Microbiota Harmony in Maintaining a Functional Skin Barrier. J Drugs Dermatol 16: 12–8.
    View at Publisher | View at Google Scholar
  11. Zeeuwen PL, Boekhorst J, van den Bogaard EH, de Koning HD, van de Kerkhof PM, Saulnier DM, et al (2012). Microbiome dynamics of human epidermis following skin barrier disruption. Genome Biol 13:R101.
    View at Publisher | View at Google Scholar
  12. Weyrich LS, Dixit S, Farrer AG, Cooper AJ, Cooper AJ (2015). The skin microbiome: Associations between altered microbial communities and disease. Australas J Dermatol 56: 268–74.
    View at Publisher | View at Google Scholar
  13. De Paepe K, Houben E, Adam R, Rogiers V (2007). Effective protection of the nasolabial skin by using lotionimpregnated paper handkerchiefs during an acute viral cold. J Cosmet Sci 29: 219–238. Symposium abstract 013 p. 223.
    View at Publisher | View at Google Scholar
  14. INCQS/FioCruz (1992). Padronização dos Testes de Sensibilidade a Antimicrobianos – Método Anvisa/CLSI (M2-A8, Vol. 23 n°1). Método para a avaliação da atividade inibitória de preparação nas formas líquida, cremosa e sólida, Manual de Saneantes, Método de difusão em placas de ágar.
    View at Publisher | View at Google Scholar
  15. Gradim AJ, Anjos M (2015). Guia de Microbiologia 1ed. Disponível em: https://abihpec.org.br/guia-microbiologia/files/assets/basic-html/page75.html. Acesso em: 16 janeiro.
    View at Publisher | View at Google Scholar
  16. Rios JL, Recio MC, Villar A (1988). Screening methods for natural products with antimicrobial activity: a review of the literature. J Ethnopharmacol 23:127-149.
    View at Publisher | View at Google Scholar
  17. Brandwein M, Steinberg D, Meshner S (2016). Microbial biofilms and the human skin microbiome. NPJ Biofilms Microbiomes 2:3.
    View at Publisher | View at Google Scholar
  18. Eberlin S, Facchini G, Silva GH, Pinheiro ALTA, Pinheiro AS (2020). Metodologías para evaluar la eficacia de los productos en la regulación de la microbiota cutânea Arte y Ciencia Cosmética 32(77): 74- 81. ISSN 0122-8072.
    View at Publisher | View at Google Scholar
  19. Eberlin S, Facchini G, Silva GH, Eberlin S, Bragatto AR, Pinheiro ALTA, Pinheiro AS (2021). Ex vivo Human skin model: an alternative test system for skin irritation and corrosion assays. ATLA (no prello).
    View at Publisher | View at Google Scholar
  20. Eberlin S, Facchini G, Silva MS, Silva GH, Pinheiro ALTA, Costa A (2020). Ex vivo Human skin model as a tool for atopic dermatitis assessment. J Am Acad Dermatol, 83(6) Supplement 1, AB161.
    View at Publisher | View at Google Scholar
  21. Eberlin S, Silva MS, Facchini G, Silva GH, Pinheiro ALTA, Eberlin S, Pinheiro AS (2020). The ex Vivo skin model as an alternative tool for the efficacy and safety evaluation of topical products. ATLA 48(1):10-22.
    View at Publisher | View at Google Scholar
  22. Harding CR, Scott IR. (2002) Stratum corneum moisturizing factors. In: J Leyden, A Rawlings, eds. Skin Moisturization. New York: Marcel Dekker: pp. 61-80.
    View at Publisher | View at Google Scholar
  23. Kam E, Resing KA, Lim S, Dale BA (1993). Identification of rat epidermal profilaggrin phosphatase as a member of the protein phosphatase 2A family. J Cell Sci 106: 219-226.
    View at Publisher | View at Google Scholar
  24. Haugen-Scofield J, Resing KA, Dale BA (1988). Characterization of an epidermal phosphatase specific for filaggrin phosphorylated by casein kinase II. J Invest Dermatol 91:533-559.
    View at Publisher | View at Google Scholar
  25. Resing KA, Walsh KA, Dale BA (1984). Identification of two intermediates during processing of profilaggrin to filaggrin in neonatalmouse epidermis. J Cell Biol 99:1372-1378.
    View at Publisher | View at Google Scholar
  26. Resing KA, Walsh KA, Haugen-Scofield J, Dale BA (1989). Identification of proteolytic cleavage sites in the conversion of profilaggrin to filaggrin in mammalian epidermis. J Biol Chem 264: 1837-1845.
    View at Publisher | View at Google Scholar
  27. Harding CR, Scott IR (1983). Histidine-rich proteins (filaggrins). Structural and functional heterogeneity during epidermal differentiation. J Mol Biol 170: 651-673.
    View at Publisher | View at Google Scholar
  28. Yosipovitch G, Yarnitzky D (1997). Quantitative sensory testing. In: Dermatotoxicology Methods: The Laboratory Worker‟s Vade Mecum; Maibach, H.I., Marzulli, F.N., Eds.; Taylor & Francis: New York, pp. 120-135.
    View at Publisher | View at Google Scholar
  29. Pons-Guiraud A (2004). Sensitive skin: a complex and multifactorial syndrome. J. Cosm. Dermatol 3, 145-148.
    View at Publisher | View at Google Scholar
  30. Costa A, Eberlin S, Polettini AJ, Pereira AFC, Pereira CS, Ferreira NMC, et al (2014). Neuromodulatory and Anti-Inflammatory Ingredient for Sensitive Skin: In Vitro Assessment. IADT 13:191-198.
    View at Publisher | View at Google Scholar
  31. Dreborg S (2001). Histamine reactivity of the skin. Allergy 56:359-364.
    View at Publisher | View at Google Scholar
  32. Columbo M, Horowitz EM, Kagey-Sobotka A, Lichtenstein LM (1996). Substance P activates the release of histamine from human skin mast cells through a pertussis toxin-sensitive and protein kinase c- dependent mechanism. Clin Immunol Immunopathol 81(1):68-73.
    View at Publisher | View at Google Scholar
  33. Douglas AD, Leeman SE (2011). Neurokinin-1 receptor: functional significance in the immune system in reference to selected infections and inflammation. Ann N Y Acad Sci 1217: 83-95.
    View at Publisher | View at Google Scholar
  34. Benedetto A, Yoshida T, Fridy S, Park JES, Kuo IH, Beck LA (2015). Histamine and skin barrier: are histamine antagonists useful for the prevention or treatment of atopic dermatitis? J Clin Med 4, 741- 755.
    View at Publisher | View at Google Scholar
  35. Kubo Y, Nakano K (1999). Regulation of histamine synthesis in mouse CD4+ and CD8+ T lymphocytes. Inflamm Res 48, 149-153.
    View at Publisher | View at Google Scholar
  36. Malaviya R, Morrison AR, Pentland AP (1996). Histamine in human epidermal cells is induced by ultraviolet light injury. J Inv Dermatol 106, 785-789.
    View at Publisher | View at Google Scholar
  37. Glatzer F, Gschwandtner M, Ehling S, Rossbach K, Janik K, Klos, et al (2013) Histamine induces proliferation in keratinocytes from patients with atopic dermatitis through the histamine 4 receptor. J Allergy Clin Immunol 132, 1358-1367.
    View at Publisher | View at Google Scholar
  38. Akdis CA, Simons FE (2006). Histamine receptors are hot in immunopharmacology. Eur J Pharmacol 533, 69-76.
    View at Publisher | View at Google Scholar
  39. Bielory L, Ghafoor S (2005). Histamine receptors and the conjunctiva. Curr Opin Allergy Clin Immunol 5, 437-440.
    View at Publisher | View at Google Scholar
  40. Gschwandtner M, Mildner M, Mlitz V, Gruber F, Eckhart L, Werfel, T, et al (2013). Histamine suppresses epidermal keratinocyte differentiation and impairs skin barrier function in a human skin model. Allergy 68, 37-47.
    View at Publisher | View at Google Scholar
  41. Keen MA, Hassan I (2016). Vitamin E in Dermatology. Indian Dermatol Online J 7(4): 311-315.
    View at Publisher | View at Google Scholar
  42. Nachbar F, Korting HC (1995). The role of vitamin-E in normal and damaged skin. J Mol Med 73, 7- 17.
    View at Publisher | View at Google Scholar
  43. Jiang Q, Christen S, Shigenaga MK, Ames BN (2001). Gamma-tocopherol, the major form of vitamin E in the US diet, deserves more attention. Am J Clin Nutr 74:714-722.
    View at Publisher | View at Google Scholar
  44. Yoshida E, Watanabe T, Takata J, Yamazaki A, Karube Y, Kobayashi S (2006). Topical application of a novel, hydrophilic gamma-tocopherol derivative reduces photo- inflammation in mice skin. J Invest Dermatol 126:1633-1640.
    View at Publisher | View at Google Scholar
  45. Beharka AA, Wu D, Serafini M, Meydani SN (2002). Mechanism of vitamin E inhibition of cyclooxygenase activity in macrophages from old mice: Role of peroxynitrite. Free Radic Biol Med 32:503-511.
    View at Publisher | View at Google Scholar
  46. Tsoureli-Nikita E, Hercogova J, Lotti T, Menchini G (2002). Evaluation of dietary intake of vitamin E in the treatment of atopic dermatitis: A study of the clinical course and evaluation of the immunoglobulin E serum levels. Int J Dermato 41:146-150.
    View at Publisher | View at Google Scholar
  47. Pallast EG, Schouten EG, de Waart FG, Fonk HC, Doekes G, von Blomberg BM, et al (1999). Effect of 50and 100-mg vitamin E supplements on cellular immune function in noninstitutionalized elderly persons. Am J Clin Nutr 69(6):1273-1281.
    View at Publisher | View at Google Scholar
  48. Reiter E, Jiang Q, Christen S (2007). Anti-inflammatory properties of α- and γ-tocopherol. Mol Aspects Med 28(5-6): 668-691.
    View at Publisher | View at Google Scholar
  49. Konger RL (2006). A new wrinkle on topical vitamin E and photo-inflammation: mechanistic studies of a hydrophilic γ-tocopherol derivative compared with α-tocopherol. J Inv Dermatol 126, 1447-1449.
    View at Publisher | View at Google Scholar
  50. Feily A, Namazi MR (2009). Aloe vera in dermatology: a brief review. G Ital Dermatol Venereol 144(1):85-91.
    View at Publisher | View at Google Scholar
  51. Maenthai song R, Chaiyakunapruk N, Niruntraporn S, Kongkaew C (2007). The efficacy of aloe vera used for burn wound healing: A systematic review. Burns 33:713-718.
    View at Publisher | View at Google Scholar
  52. Hęś M, Dziedzic K, Górecka D, Jędrusek-Golińska A, Gujska E (2019). Aloe vera (L.) Webb. Natural Sources of Antioxidants – A Review. Plant Foods for Hum Nutr 74:255-265.
    View at Publisher | View at Google Scholar
  53. Ray A, Aswatha SM (2013). An analysis of the influence of growth periods on physical appearance, and acemannan and elemental distribution of Aloe vera L.gel. Ind Crop Prod 48:36-42.
    View at Publisher | View at Google Scholar
  54. Tanaka M, Yamamoto Y, Misawa E, Nabeshima K, Saito M, Yamauchi K, et al (2016). Effects of Aloe sterol supplementation on skin elasticity, hydration, and collagen score: a 12-week double-blind, randomized, controlled trial. Skin Pharmacol Physiol 29:309-317.
    View at Publisher | View at Google Scholar
  55. Hekmatpou D, Mehrabi F, Rahzani K, Aminiyan A (2019). The effect of aloe vera clinical trials on prevention and healing of skin wound: a systematic review. Iran J Med Sci 44(1):1-9.
    View at Publisher | View at Google Scholar
  56. Dal'Belo SE, Gaspar LR, Campos PMBGM (2006). Moisturizing effect of cosmetic formulations containing Aloe vera extract in different concentrations assessed by skin bioengineering techniques. Skin Res Technol 12(4):241-6.
    View at Publisher | View at Google Scholar
  57. Srivastava JK, Shankar E, Gupta S (2010). Chamomile: A herbal medicine of the past with bright future. Mol Med Report 3(6): 895-901.
    View at Publisher | View at Google Scholar
  58. Astin JA, Pelletier KR, Marie A, Haskell WL (2000). Complementary and alternative medicine use among elderly persons: one year analysis of blue shield medicare supplement. J Gerontol 5:M4-M9.
    View at Publisher | View at Google Scholar
  59. Sabale V, Kunjwani H, Sabale P (2011). Formulation and in vitro evaluation of the topical antiageing preparation of the fruit of Benincasa hispida. J Ayurveda and Integr Med 2(3): 124-128.
    View at Publisher | View at Google Scholar
  60. Jadoon S, Karim S, Asad MHHB, Akram MR, Kalsoom A, Khan AK, et al (2015). Anti-aging potential of phytoextract loaded-pharmaceutical creams for human skin cell longetivity. oxidative medicine and cellular longevity, Article ID 709628, 17 pages.
    View at Publisher | View at Google Scholar
  61. Mahmood T, Akhtar N, Khan BA, Khan HMS,Saeed T (2010). Outcomes of 3% green tea emulsion on skin sebum production in male volunteers. Bosnian J Bas Med Sci 10(3): 260-264.
    View at Publisher | View at Google Scholar
  62. Mahmood T, Akhtar N, Khan BA, Ahmad M, Khan HMS, Zaman SU (2010). Application of stable green tea extract cream on human cheeks. Int J Acad Res 2(2): 121-126.
    View at Publisher | View at Google Scholar
  63. Almeida IF, Maleckova J, Saffi R, Monteiro H, Góios F, Amaral MH et al (2013). Characterization of an antioxidant surfactant-free topical formulation containing Castanea sativa leaf extract. Drug Dev Ind Pharm 41(1):148-155.
    View at Publisher | View at Google Scholar
  64. McDaniel DH (2009). Clinical safety and efficacy in photoaged skin with CoffeeBerry extract, a natural antioxidant. Cosm Dermatol 22(12): 610-616.
    View at Publisher | View at Google Scholar
  65. Ali A, Akhtar N, Chowdhary F (2014). Enhancement of human skin facial revitalization by moringa leaf extract cream. Postepy Dermatologii i Alergologii 3(2): 71-76.
    View at Publisher | View at Google Scholar
  66. Akhtar N, Hisham J, Khan HMS, Khan BA, Mahmood T, Saeed T (2012). Whitening and antierythemic effect of a cream containing Morus alba extract. Hygeia J Drugs and Med 4(1): 97-103, 2012.
    View at Publisher | View at Google Scholar
  67. Waqas MK, Akhtar N, Khan HMS, Mustafa R, Murtaza G (2014). Stability study of a cosmetic emulsion loaded with Tamarindus indica seeds extract. Latin Am J of Pharm 33(5): 731-738.
    View at Publisher | View at Google Scholar

Clearly Auctoresonline and particularly Psychology and Mental Health Care Journal is dedicated to improving health care services for individuals and populations. The editorial boards' ability to efficiently recognize and share the global importance of health literacy with a variety of stakeholders. Auctoresonline publishing platform can be used to facilitate of optimal client-based services and should be added to health care professionals' repertoire of evidence-based health care resources.

img

Virginia E. Koenig

Journal of Clinical Cardiology and Cardiovascular Intervention The submission and review process was adequate. However I think that the publication total value should have been enlightened in early fases. Thank you for all.

img

Delcio G Silva Junior

Journal of Women Health Care and Issues By the present mail, I want to say thank to you and tour colleagues for facilitating my published article. Specially thank you for the peer review process, support from the editorial office. I appreciate positively the quality of your journal.

img

Ziemlé Clément Méda

Journal of Clinical Research and Reports I would be very delighted to submit my testimonial regarding the reviewer board and the editorial office. The reviewer board were accurate and helpful regarding any modifications for my manuscript. And the editorial office were very helpful and supportive in contacting and monitoring with any update and offering help. It was my pleasure to contribute with your promising Journal and I am looking forward for more collaboration.

img

Mina Sherif Soliman Georgy

We would like to thank the Journal of Thoracic Disease and Cardiothoracic Surgery because of the services they provided us for our articles. The peer-review process was done in a very excellent time manner, and the opinions of the reviewers helped us to improve our manuscript further. The editorial office had an outstanding correspondence with us and guided us in many ways. During a hard time of the pandemic that is affecting every one of us tremendously, the editorial office helped us make everything easier for publishing scientific work. Hope for a more scientific relationship with your Journal.

img

Layla Shojaie

The peer-review process which consisted high quality queries on the paper. I did answer six reviewers’ questions and comments before the paper was accepted. The support from the editorial office is excellent.

img

Sing-yung Wu

Journal of Neuroscience and Neurological Surgery. I had the experience of publishing a research article recently. The whole process was simple from submission to publication. The reviewers made specific and valuable recommendations and corrections that improved the quality of my publication. I strongly recommend this Journal.

img

Orlando Villarreal

Dr. Katarzyna Byczkowska My testimonial covering: "The peer review process is quick and effective. The support from the editorial office is very professional and friendly. Quality of the Clinical Cardiology and Cardiovascular Interventions is scientific and publishes ground-breaking research on cardiology that is useful for other professionals in the field.

img

Katarzyna Byczkowska

Thank you most sincerely, with regard to the support you have given in relation to the reviewing process and the processing of my article entitled "Large Cell Neuroendocrine Carcinoma of The Prostate Gland: A Review and Update" for publication in your esteemed Journal, Journal of Cancer Research and Cellular Therapeutics". The editorial team has been very supportive.

img

Anthony Kodzo-Grey Venyo

Testimony of Journal of Clinical Otorhinolaryngology: work with your Reviews has been a educational and constructive experience. The editorial office were very helpful and supportive. It was a pleasure to contribute to your Journal.

img

Pedro Marques Gomes

Dr. Bernard Terkimbi Utoo, I am happy to publish my scientific work in Journal of Women Health Care and Issues (JWHCI). The manuscript submission was seamless and peer review process was top notch. I was amazed that 4 reviewers worked on the manuscript which made it a highly technical, standard and excellent quality paper. I appreciate the format and consideration for the APC as well as the speed of publication. It is my pleasure to continue with this scientific relationship with the esteem JWHCI.

img

Bernard Terkimbi Utoo

This is an acknowledgment for peer reviewers, editorial board of Journal of Clinical Research and Reports. They show a lot of consideration for us as publishers for our research article “Evaluation of the different factors associated with side effects of COVID-19 vaccination on medical students, Mutah university, Al-Karak, Jordan”, in a very professional and easy way. This journal is one of outstanding medical journal.

img

Prof Sherif W Mansour

Dear Hao Jiang, to Journal of Nutrition and Food Processing We greatly appreciate the efficient, professional and rapid processing of our paper by your team. If there is anything else we should do, please do not hesitate to let us know. On behalf of my co-authors, we would like to express our great appreciation to editor and reviewers.

img

Hao Jiang

As an author who has recently published in the journal "Brain and Neurological Disorders". I am delighted to provide a testimonial on the peer review process, editorial office support, and the overall quality of the journal. The peer review process at Brain and Neurological Disorders is rigorous and meticulous, ensuring that only high-quality, evidence-based research is published. The reviewers are experts in their fields, and their comments and suggestions were constructive and helped improve the quality of my manuscript. The review process was timely and efficient, with clear communication from the editorial office at each stage. The support from the editorial office was exceptional throughout the entire process. The editorial staff was responsive, professional, and always willing to help. They provided valuable guidance on formatting, structure, and ethical considerations, making the submission process seamless. Moreover, they kept me informed about the status of my manuscript and provided timely updates, which made the process less stressful. The journal Brain and Neurological Disorders is of the highest quality, with a strong focus on publishing cutting-edge research in the field of neurology. The articles published in this journal are well-researched, rigorously peer-reviewed, and written by experts in the field. The journal maintains high standards, ensuring that readers are provided with the most up-to-date and reliable information on brain and neurological disorders. In conclusion, I had a wonderful experience publishing in Brain and Neurological Disorders. The peer review process was thorough, the editorial office provided exceptional support, and the journal's quality is second to none. I would highly recommend this journal to any researcher working in the field of neurology and brain disorders.

img

Dr Shiming Tang

Dear Agrippa Hilda, Journal of Neuroscience and Neurological Surgery, Editorial Coordinator, I trust this message finds you well. I want to extend my appreciation for considering my article for publication in your esteemed journal. I am pleased to provide a testimonial regarding the peer review process and the support received from your editorial office. The peer review process for my paper was carried out in a highly professional and thorough manner. The feedback and comments provided by the authors were constructive and very useful in improving the quality of the manuscript. This rigorous assessment process undoubtedly contributes to the high standards maintained by your journal.

img

Raed Mualem

International Journal of Clinical Case Reports and Reviews. I strongly recommend to consider submitting your work to this high-quality journal. The support and availability of the Editorial staff is outstanding and the review process was both efficient and rigorous.

img

Andreas Filippaios

Thank you very much for publishing my Research Article titled “Comparing Treatment Outcome Of Allergic Rhinitis Patients After Using Fluticasone Nasal Spray And Nasal Douching" in the Journal of Clinical Otorhinolaryngology. As Medical Professionals we are immensely benefited from study of various informative Articles and Papers published in this high quality Journal. I look forward to enriching my knowledge by regular study of the Journal and contribute my future work in the field of ENT through the Journal for use by the medical fraternity. The support from the Editorial office was excellent and very prompt. I also welcome the comments received from the readers of my Research Article.

img

Dr Suramya Dhamija

Dear Erica Kelsey, Editorial Coordinator of Cancer Research and Cellular Therapeutics Our team is very satisfied with the processing of our paper by your journal. That was fast, efficient, rigorous, but without unnecessary complications. We appreciated the very short time between the submission of the paper and its publication on line on your site.

img

Bruno Chauffert

I am very glad to say that the peer review process is very successful and fast and support from the Editorial Office. Therefore, I would like to continue our scientific relationship for a long time. And I especially thank you for your kindly attention towards my article. Have a good day!

img

Baheci Selen

"We recently published an article entitled “Influence of beta-Cyclodextrins upon the Degradation of Carbofuran Derivatives under Alkaline Conditions" in the Journal of “Pesticides and Biofertilizers” to show that the cyclodextrins protect the carbamates increasing their half-life time in the presence of basic conditions This will be very helpful to understand carbofuran behaviour in the analytical, agro-environmental and food areas. We greatly appreciated the interaction with the editor and the editorial team; we were particularly well accompanied during the course of the revision process, since all various steps towards publication were short and without delay".

img

Jesus Simal-Gandara

I would like to express my gratitude towards you process of article review and submission. I found this to be very fair and expedient. Your follow up has been excellent. I have many publications in national and international journal and your process has been one of the best so far. Keep up the great work.

img

Douglas Miyazaki

We are grateful for this opportunity to provide a glowing recommendation to the Journal of Psychiatry and Psychotherapy. We found that the editorial team were very supportive, helpful, kept us abreast of timelines and over all very professional in nature. The peer review process was rigorous, efficient and constructive that really enhanced our article submission. The experience with this journal remains one of our best ever and we look forward to providing future submissions in the near future.

img

Dr Griffith

I am very pleased to serve as EBM of the journal, I hope many years of my experience in stem cells can help the journal from one way or another. As we know, stem cells hold great potential for regenerative medicine, which are mostly used to promote the repair response of diseased, dysfunctional or injured tissue using stem cells or their derivatives. I think Stem Cell Research and Therapeutics International is a great platform to publish and share the understanding towards the biology and translational or clinical application of stem cells.

img

Dr Tong Ming Liu

I would like to give my testimony in the support I have got by the peer review process and to support the editorial office where they were of asset to support young author like me to be encouraged to publish their work in your respected journal and globalize and share knowledge across the globe. I really give my great gratitude to your journal and the peer review including the editorial office.

img

Husain Taha Radhi

I am delighted to publish our manuscript entitled "A Perspective on Cocaine Induced Stroke - Its Mechanisms and Management" in the Journal of Neuroscience and Neurological Surgery. The peer review process, support from the editorial office, and quality of the journal are excellent. The manuscripts published are of high quality and of excellent scientific value. I recommend this journal very much to colleagues.

img

S Munshi

Dr.Tania Muñoz, My experience as researcher and author of a review article in The Journal Clinical Cardiology and Interventions has been very enriching and stimulating. The editorial team is excellent, performs its work with absolute responsibility and delivery. They are proactive, dynamic and receptive to all proposals. Supporting at all times the vast universe of authors who choose them as an option for publication. The team of review specialists, members of the editorial board, are brilliant professionals, with remarkable performance in medical research and scientific methodology. Together they form a frontline team that consolidates the JCCI as a magnificent option for the publication and review of high-level medical articles and broad collective interest. I am honored to be able to share my review article and open to receive all your comments.

img

Tania Munoz

“The peer review process of JPMHC is quick and effective. Authors are benefited by good and professional reviewers with huge experience in the field of psychology and mental health. The support from the editorial office is very professional. People to contact to are friendly and happy to help and assist any query authors might have. Quality of the Journal is scientific and publishes ground-breaking research on mental health that is useful for other professionals in the field”.

img

George Varvatsoulias

Dear editorial department: On behalf of our team, I hereby certify the reliability and superiority of the International Journal of Clinical Case Reports and Reviews in the peer review process, editorial support, and journal quality. Firstly, the peer review process of the International Journal of Clinical Case Reports and Reviews is rigorous, fair, transparent, fast, and of high quality. The editorial department invites experts from relevant fields as anonymous reviewers to review all submitted manuscripts. These experts have rich academic backgrounds and experience, and can accurately evaluate the academic quality, originality, and suitability of manuscripts. The editorial department is committed to ensuring the rigor of the peer review process, while also making every effort to ensure a fast review cycle to meet the needs of authors and the academic community. Secondly, the editorial team of the International Journal of Clinical Case Reports and Reviews is composed of a group of senior scholars and professionals with rich experience and professional knowledge in related fields. The editorial department is committed to assisting authors in improving their manuscripts, ensuring their academic accuracy, clarity, and completeness. Editors actively collaborate with authors, providing useful suggestions and feedback to promote the improvement and development of the manuscript. We believe that the support of the editorial department is one of the key factors in ensuring the quality of the journal. Finally, the International Journal of Clinical Case Reports and Reviews is renowned for its high- quality articles and strict academic standards. The editorial department is committed to publishing innovative and academically valuable research results to promote the development and progress of related fields. The International Journal of Clinical Case Reports and Reviews is reasonably priced and ensures excellent service and quality ratio, allowing authors to obtain high-level academic publishing opportunities in an affordable manner. I hereby solemnly declare that the International Journal of Clinical Case Reports and Reviews has a high level of credibility and superiority in terms of peer review process, editorial support, reasonable fees, and journal quality. Sincerely, Rui Tao.

img

Rui Tao

Clinical Cardiology and Cardiovascular Interventions I testity the covering of the peer review process, support from the editorial office, and quality of the journal.

img

Khurram Arshad

Clinical Cardiology and Cardiovascular Interventions, we deeply appreciate the interest shown in our work and its publication. It has been a true pleasure to collaborate with you. The peer review process, as well as the support provided by the editorial office, have been exceptional, and the quality of the journal is very high, which was a determining factor in our decision to publish with you.

img

Gomez Barriga Maria Dolores

The peer reviewers process is quick and effective, the supports from editorial office is excellent, the quality of journal is high. I would like to collabroate with Internatioanl journal of Clinical Case Reports and Reviews journal clinically in the future time.

img

Lin Shaw Chin

Clinical Cardiology and Cardiovascular Interventions, I would like to express my sincerest gratitude for the trust placed in our team for the publication in your journal. It has been a true pleasure to collaborate with you on this project. I am pleased to inform you that both the peer review process and the attention from the editorial coordination have been excellent. Your team has worked with dedication and professionalism to ensure that your publication meets the highest standards of quality. We are confident that this collaboration will result in mutual success, and we are eager to see the fruits of this shared effort.

img

Maria Dolores Gomez Barriga

Dear Dr. Jessica Magne, Editorial Coordinator 0f Clinical Cardiology and Cardiovascular Interventions, I hope this message finds you well. I want to express my utmost gratitude for your excellent work and for the dedication and speed in the publication process of my article titled "Navigating Innovation: Qualitative Insights on Using Technology for Health Education in Acute Coronary Syndrome Patients." I am very satisfied with the peer review process, the support from the editorial office, and the quality of the journal. I hope we can maintain our scientific relationship in the long term.

img

Dr Maria Dolores Gomez Barriga

Dear Monica Gissare, - Editorial Coordinator of Nutrition and Food Processing. ¨My testimony with you is truly professional, with a positive response regarding the follow-up of the article and its review, you took into account my qualities and the importance of the topic¨.

img

Dr Maria Regina Penchyna Nieto

Dear Dr. Jessica Magne, Editorial Coordinator 0f Clinical Cardiology and Cardiovascular Interventions, The review process for the article “The Handling of Anti-aggregants and Anticoagulants in the Oncologic Heart Patient Submitted to Surgery” was extremely rigorous and detailed. From the initial submission to the final acceptance, the editorial team at the “Journal of Clinical Cardiology and Cardiovascular Interventions” demonstrated a high level of professionalism and dedication. The reviewers provided constructive and detailed feedback, which was essential for improving the quality of our work. Communication was always clear and efficient, ensuring that all our questions were promptly addressed. The quality of the “Journal of Clinical Cardiology and Cardiovascular Interventions” is undeniable. It is a peer-reviewed, open-access publication dedicated exclusively to disseminating high-quality research in the field of clinical cardiology and cardiovascular interventions. The journal's impact factor is currently under evaluation, and it is indexed in reputable databases, which further reinforces its credibility and relevance in the scientific field. I highly recommend this journal to researchers looking for a reputable platform to publish their studies.

img

Dr Marcelo Flavio Gomes Jardim Filho

Dear Editorial Coordinator of the Journal of Nutrition and Food Processing! "I would like to thank the Journal of Nutrition and Food Processing for including and publishing my article. The peer review process was very quick, movement and precise. The Editorial Board has done an extremely conscientious job with much help, valuable comments and advices. I find the journal very valuable from a professional point of view, thank you very much for allowing me to be part of it and I would like to participate in the future!”

img

Zsuzsanna Bene

Dealing with The Journal of Neurology and Neurological Surgery was very smooth and comprehensive. The office staff took time to address my needs and the response from editors and the office was prompt and fair. I certainly hope to publish with this journal again.Their professionalism is apparent and more than satisfactory. Susan Weiner

img

Dr Susan Weiner

My Testimonial Covering as fellowing: Lin-Show Chin. The peer reviewers process is quick and effective, the supports from editorial office is excellent, the quality of journal is high. I would like to collabroate with Internatioanl journal of Clinical Case Reports and Reviews.

img

Lin-Show Chin

My experience publishing in Psychology and Mental Health Care was exceptional. The peer review process was rigorous and constructive, with reviewers providing valuable insights that helped enhance the quality of our work. The editorial team was highly supportive and responsive, making the submission process smooth and efficient. The journal's commitment to high standards and academic rigor makes it a respected platform for quality research. I am grateful for the opportunity to publish in such a reputable journal.

img

Sonila Qirko

My experience publishing in International Journal of Clinical Case Reports and Reviews was exceptional. I Come forth to Provide a Testimonial Covering the Peer Review Process and the editorial office for the Professional and Impartial Evaluation of the Manuscript.

img

Luiz Sellmann