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*Corresponding Author: Musa bashir, Department of Animal Science, Kano State University of Science & Tech, Wudil.
Citation: M bashir, J O. Alagbe, A M Betty, Omokore E.A. (2020) Growth Performance, Caeca Microbial Population And Immune Response Of Starter Broiler Chicks Fed Aqueous Extract Of Balanites Aegyptiaca And Alchornea Cordifolia Stem Bark Mixture. Biomedical Research and Clinical Reviews. 1(4); DOI: 10.31579/2692-9406/022
Copyright: © 2020 Musa bashir, This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Received: 03 September 2020 | Accepted: 10 October 2020 | Published: 30 October 2020
Keywords: broiler chicks; immune response; performance; antibodies
A total of Two hundred and fifty (250), 1-day old (Cobb) broiler chicks with mixed sex were used to evaluate the the growth performance, caeca microbial population and immune response of starter broiler chicks fed aqueous extract of Balanites aegyptiaca and Alchornea cordifolia stem bark mixture (BACM). Birds were reared on a deep litter system and randomly divided into five treatment with five replicates consisting of 10 birds each in a completely randomized design. Treatment 1 (T1) were given basal diet + 0 % BACM, T2, T3, T4 and T5 were fed 20, 40, 60 and 80 ml/liter BACM respectively. The experiment lasted for 28 during which clean feed and water were offered ad libitum. The results obtained revealed that the average weight gain (AWG), feed conversion ratio (FCR) and mortality were influenced by the dietary treatments (P<0.05). Birds in T5 had the highest AWG and FCR (1159.3 g, 1.57) followed by T4 (1070.2 g, 1.70), T3 (1047.4 g, 1.74), T2 (981.1 g, 1.86) and T1 (850.7 g, 2.14) respectively. Activities of superoxide dismutase (SDA), glutathione peroxidase (GPx), catalase (CAT) ,malonyldialdehyde (MLA) and antibody titres against Newcastle and gumboro disease were significantly affected by BACM (P<0.05). Caeca microbial population of Escherichia coli and Lactobacilli were significantly different among the treatments (P<0.05). E. coli count in T1 were higher compared to other treatments (P<0.05), Lactobacilli population increased in T2, T3, T4 and T5 compared to T1. It was concluded that BACM can be fed to broiler chicks at 80 ml/litre without any negetive effect on the performance and immune response of birds.
The use of medicinal plants of high therapeutic value have recently gained interest since the ban on the use of antibiotics by the European Union in 2006 due to anticipated toxicity, high cost and adverse effect (Adu et al., 2009; Oluwafemi et al., 2020). According to Mahima et al. (2012); Ezekiel et al. (2019), there are over 200,000 species of medicinal plant species where about 800 plant species have been used by different communities for curing different diseases and they contain several minerals, vitamins, protein and essential fatty acids. Among the potential medicinal plants are Balanites aegyptiaca and Alchornea cordifolia, they contains several bioactive chemicals or phytochemicals which allows them to perform multiple biological activities. The plants are found to contain alkaloids, flavonoids, phenols, saponins, tannins, oxalate, terpenoids, steroids etc. (Alagbe et al., 2020; Abu et al., 2016) which confers them ability to function as an antimicrobial (Ajetumobi, 2014), anti-inflammatory (Agyare et al., 2014), antiviral (Audu et al., 2014), antioxidant (Ojo et al., 2006), anti-ulcer, antihelminthic and anti-implantation (Francis et al., 2002; Chothani and Vaghasiya, 2011).
Balanites aegyptiaca belongs to the family Zygophyllaceae. It’s found in many parts of Africa (Kenya, Sudan, Somalia, Djibouti, Nigeria and Ethiopia) and Asia (China, India, Pakistan, Afghanistan, Sri Lanka and Bangladesh) (Dubey et al., 2011). The leaves are characterized by dark green or grey green colouration with two firm coriaceous leaflets spirally arranged on the shoots (Chothani and Vaghasiya, 2011). The leaf, stem bark and roots are traditionally used to treat headache, stomach disorder, wound; skin infection and tooth ache (Sunil et al., 2016). Phytochemical evaluation of the stem bark, leaves and roots revealed the presence of appreciable quantity of flavonoids, alkaloids, saponins, phenols, terpenoids, tannins glycosides, amino acids, vitamins, carbohydrates and protein (Sunil et al., 2016).
Alchornea cordifolia (Euphorbiaceae) is a perennial evergreen tree which measures between 4-8 m high, grows near river bank or marshy places and it is characterized by unisexual and sessile flowers (Timibitei et al., 2013) and wide spread in Kenya, Senegal, Nigeria, Niger, Cameroon, Tanzania, Angola, Mudagaska, China and some parts of India (Kwabena, 2012). Mamadou et al. (2005); Noundou (2012); Osabe and Okoye (2003) reported that A. cordifolia is loaded with several secondary metabolites (terpenoids, alkaloids, phenols, steroids, glycosides, hydroxybenzoic acid, tannins, imidazopyrimidine, alchorneine, namelygallic acid, anthralinic acid, ellagic acid, alchornidine guanidine, nutrients (carbohydrate, protein and amino acids) and have traditionally used to treat rheumatism, arthritis, tooth ache and pile.
Previous studies have shown that the immune system benefit greatly from proper nutrition (phytogenics) of the bird (Gary, 2002; Mahima et al., 2012). Phytogenics is a safe growth promoter without side effects on birds (Alagawany et al., 2016), enhance the modulation of beneficial intestinal microbiota by controlling potential pathogens (Alagbe, 2020; Oyuntsetseg et al., 2014; Farag et al., 2016) and improvement of nutrient absorption and enzyme activity to enhance better weight gain and feed conversion efficiency (Santi and Kim, 2017) due to the presence of several bioactive chemicals.
In view of these potential a synergistic combination of different plants will give better result, especially those that are underexplored. Therefore the experiment was carried out to evaluate the growth performance, caeca microbial population and immune response of starter broiler chicks fed aqueous extract of Balanites aegyptiaca and Alchornea cordifolia stem bark mixture.
EXPERIMENTAL SITE
The experiment was carried at Kano State University of Science and Technology, Wudil, Kano State, Nigeria.
COLLECTION, PROCESSING, PREPARATION OF EXTRACT AND ANALYSIS
Healthy stems of Balanites aegyptiaca and Alchornea cordifolia were obtained from the Teaching and Research farm of Kano State University, Nigeria. The plant materials were identified and authenticated by a botanist (Dr. Bashir), and thoroughly washed with distilled water to remove soil and other bound particles, air dried separately until a constant weight was obtained and made into powder using a pulverizer. Samples were later stored in a well labeled air tight container and kept for further analysis. 100 g of each ground sample (Balanites aegyptiaca and Alchornea cordifolia) were mixed together (1:1) dissolved in 1000 ml water, stirred continuously and kept in the refrigerator for 48 hours. The extract was filtered using Whatman filter paper No. 1 to obtain filtrate (BACM).
Proximate compositions of test material and experiment diet were determined by using official method of analysis by AOAC (2000).
Phytochemical evaluation of tannins, alkaloids, saponins, flavonoids, phenols, oxalate, glycosides, steroids and terpenoids were estimated using methods described by Atamgba et al. (2015), Harbone (1973), Shabbir et al. (2013), Odebiyi and Sofowora (1978), Boham and Kocipai (1974). Mineral analyses were carried out using Atomic Absorption Spectrophotometer (AAS) model 12-0TA.
ANIMALS AND THEIR MANAGEMENT
A total of two hundred and fifty one-day old broiler chicks (Cobb) strain of mixed sex were randomly distributed into five treatments with 5 replicates, each replicates contained 10 birds each in a completely randomized design. Prior to the arrival of the birds the deep litter pen house were properly disinfected and the foot bath is constructed to ensure biosecurity. Birds were weighed on arrival to the farm to determine their initial body weight and weekly thereafter. Wood shavings were used as litter material and lighting was continuous, vaccines were administered according to the prevailing disease condition in the environment and all necessary management practices were strictly adhered to, clean feed and water were offered ad libitum and the experiment lasted for 28 days.
FEED FORMULATION AND EXPERIMENTAL SET UP
A standard starter’s ration was formulated to meet the nutritional recommendation of birds by NRC (1994). It was made up of corn –soya meal based diet and it contained 23 % crude protein and 2900 Kcal/kg energy.
Treatment 1- Basal diet + 0 % BACM
Treatment 2 – Basal diet + 20 ml/liter BACM
Treatment 3 – Basal diet + 40 ml/liter BACM
Treatment 4 – Basal diet + 60 ml/liter BACM
Treatment 5 – Basal diet + 80 ml/liter BACM
EXPERIMENTAL MEASUREMENTS
Performance record
Feed intake was recorded daily and body weight gain was recorded weekly, feed conversion ratio was calculated by dividing the total feed intake by weight gain, mortality was also recorded as it occurs.
Fatty acid of the feed
Fatty acid composition of the feed was carried out using gas liquid chromatography (Model 231 A-01, Punjab, India). Percentage concentrations were evaluated according to the methods outlined by Suriya et al. (2014).
Haemagglutination inhibition test
Birds were orally vaccinated against Newcastle on the 5th and 18th day and Gumbroro diseases on the 11th and 23rd day. Three birds were randomly selected per replicate to access the antibody response to Newcastle and Gumboro virus on the 20th and 28th days of the experiment. Analysis was done according to the method described by Thayer and Beard (1998).
Caecal microbial population
At the end of the experiment (12 weeks), caeca microbial count was conducted using five (5) grasscutters per treatments, caeca contents were collected from slaughtered animal and 10-fold serial dilution method, in which of 1% peptone solution was mixed with caeca samples and poured on Mac Conkey agar plates and lactobacilli medium III agar plates, was used to determine the colony forming unit (cfu) in each gram of caeca sample by means of pour plate method. Colonies of E. coli and Lactobacilli were enumerated according to the method outlined by Phyo et al. (2017).The microbial counts were determined as colony forming units (Cfu/g) of sample.
Antioxidant status
Activity of superoxide dismutase (SDA), glutathione peroxidase (GPx), catalase (CAT) and malonyldialdehyde (MLA) were carried out using method outlined by Mahipal et al. (2015).
Statistical analysis
All data were subjected to one -way analysis of variance (ANOVA) using SPSS (23.0) and significant means were separated using Duncan multiple range tests (Duncan, 1955). Significant was declared if P ≤ 0.05.
1Total saturated fatty acid= C12:0 + C14:0 + C16:0 + C18:0 + C20:0 +C22:0
2Mono unsaturated fatty acid= C14:1C + C16:1C + C18:1C + C18:1n9t + C18:1n9c + C22:1
3Polyunsaturated fatty acid = C18:2 n6 + C20:5 n3 + C18:3n3 + C20:4n6 + C20:3n6 + C: 22:6n3
4n-6: n-3 = (C18:2 n6 + C20:4n 6 + C20:3n 6 / (C20:5n3 + C18:3n 3 + C: 22 6n 3), 5Antherogenic index = (C12:0+ 4×C14:0+ C16)/⅀ of UFA
Means in the same row with different superscripts differ significantly (P<0.05)
IBW: Initial body weight; FBW: final body weight; WG: weight gain; ADWG: average daily weight gain; FI: feed intake; ADFI: average daily feed intake; FCR: feed conversion ratio; MORT: mortality.
Means in the same row with different superscripts differ significantly (P<0.05)
SEM: Standard error of mean
Means in the same row with different superscripts differ significantly (P<0.05)
SOD, superoxide dismutase; CAT, catalase; MLA, malondialdehyde; GSH, reduced glutathione
SEM: Standard error of mean
Proximate and fatty acid composition of experimental diet
The chemical composition of experimental diet is presented in Table 1. Result revealed the presence of crude protein (23.11 %), crude fibre (3.07 %), ether extract (5.12 %), calcium (0.97 %), phosphorus (0.46 %) and energy (2990.7 Kcal/kg). Similarly, total saturated fatty (TSFA) 51.44 %, total unsaturated fatty acid (TUFA) 45.62 %, monosaturated fatty acid (MUFA) 38.06 %, omega 3 fatty acid (n-3) 1.01 %, omega 6 fatty acid (n-6) 10.22 %, n-3 : n-6 (10.11 %) and antheriogenic index (0.21 %) is presented in Table 2.
Phytochemical analysis of Balanites aegyptiaca and Alchornea cordifolia stem bark
Phytochemical composition of Balanites aegyptiaca and Alchornea cordifolia stem bark is presented in Table 3. Balanites aegyptiaca stem bark contained alkaloids (2.33 %), hydrolysable tannins (3.07 %), condensed tannins (0.01 %), flavonoids (5.84 %), saponins (0.36 %), phenols (2.88 %), terpenoids (0.60 %), steroids (1.00 %), phytates (0.07 %) and oxalates (0.05 %) while Alchornea cordifolia stem bark posses alkaloids, flavonoids, saponins, condensed tannins, hydrolysable tannins, phenols, steroids, oxalates and phytates at 0.42 %, 6.10 %, 0.22 %, 0.03 %, 2.44 %, 1.84 %, 0.04 %, 0.02 % and 0.15 % respectively.
Performance characteristics of birds fed different level of BACM
Performance characteristics of the experimental birds are presented in Table 4. IBW values ranged between (41.00 – 41.65 g), FBW (892.3 – 1200.3 g) and WG (850.7 – 1159.3 g) were higher (P<0.05) in T5 than in T4, T3, T2 and T1. FI values ranged between (1820 – 1822 g) and ADFI (65.0 – 65.08 g), however, no significant differences were observed among the treatments (P>0.05). Mortality were highest for T1 and none was recorded in the other treatments (P<0.05).
Caeca microbial population of broiler chicks given BACM
Caeca microbial population of birds fed BACM is presented in Table 5. E. coli values ranged between (9.01 – 17.80 Cfu/g) and lactobacilli (20.01 – 35.02 Cfu/g). E. coli were lowest in T5, T4, T3 and T2 and highest in T1 (P<0.05). Lactobacilli count were highest in T4 and T5 and lowest in T1 (P<0.05).
Immune and antioxidant status of broiler chicks fed BACM
The antioxidant status of the experimental birds is presented in Table 6. Whereas MLA (1.03 – 3.03 U/mgHb), SDA (21.5 – 35.0 U/mgHb) and GPx (14.5 – 28.1 U/mgHb) were lowest (P<0.05) for T1. CAT (29.5 – 41.0 U/mgHb) were highest (P<0.05) for T1 relative to other treatments.
The antibody titre as influenced by BACM is presented in Table 7. Newcastle antibody titre in day 5 ranges between 2.44 – 4.33 (Log2) while day 18 3.03 – 7.11(Log2). Parameters were significantly different among the treatments (P<0.05). Gumboro antibody titers on day 11 ranges between 1.78 – 3.00 (Log2) while on day 23 (2.56 – 5.05) (Log2) were affected by feeding BACM to birds (P<0.05).
The chemical composition of experimental diet is in agreement with the nutritional requirement of birds according to NRC (1994); Aduku (2004). This is an indication that the feed contains the entire nutrients necessary for optimum growth and immunocompetency of animals (Butcher and Miles, 2002; Makhosazana, 2015). Phytochemical composition of Balanites aegyptiaca and Alchornea cordifolia stem bark reveals the presence of several bioactive chemicals or secondary metabolites which performs multiple biological activities. The present findings coincides with other research findings from Ngaha et al. (2016); Audu et al. (2018); Onyema et al. (2017). The presence of alkaloids confers the stem bark ability to function as an antibacterial, anti-malarial and anticancer; this supports the earlier findings of Eldin et al. (2016). Flavonoids play a pivotal role as an anti-inflammatory, anti-allergic and anti-plasmodic (Dubey et al., 2011; Sunil et al., 2016). Saponin performs both antibacterial and antifungal activities (Adeshina et al., 2012; Alagbe, 2020). Phenols are strong antioxidants which prevents the entry of diseases (Ezeokeke et al., 2015; Alagbe, 2019). Terpenoids has high therapeutic value and function as antimicrobial, anticarcinogenic and anti-diuretic (Ismaila et al., 2012; Kamenan et al., 2013). Steroids play a major role in fertility of animals (Atamgba et al., 2015; Alagbe, 2019). Tannins have found therapeutic application as antiviral and antibacterial (Adisa et al., 2010). Phytate are antioxidant compounds capable of binding minerals (Maisarah et al., 2014; Akpabio and Ikpe, 2013). Bioactive chemicals in plants vary according to species, age, soil type, geographical area and method of extraction (Omokore and Alagbe, 2019). Olanipekun et al. (2016) reported a higher value of 6.78 % (alkaloids) and 2.79 % (saponins) in Morinda lucida stem bark. Enin et al. (2014) also reported a lower value of 0.60 % (tannins), 0.52 % (alkaloids), 0.31 % (flavonoids), 0.65 % (saponins), 0.14 % (oxalate) and 0.21 % (phytate) in Sida acuta. However, all the values obtained in this study were within the tolerable level reported by Olafadehan et al. (2020); Alagbe and Oluwafemi (2019).
The fatty acid in the diet shows that it’s loaded with poly unsaturated fatty acid; this removes the risk of cardiovascular infection and ensures food safety (meat). Feeding animals with the experimental diet and BACM will improve the nutritive value of the meat, since phytochemicals in plants can function as modulators, this result is consistent with the reports of Suriya et al. (2014); Alagbe (2020). Low antheriogenic index reduces the risk of artheriosclerosis (Kholif et al., 2017). Omega -3 and omega - 6 polyunsaturated fatty acid ratios was within the range recommended by Simopoulos (2001).
The improved the final live weight, total weight gain and average daily gain of birds in treatment 4 and 5 compared to the other treatments could be attributed to efficient feed utilization as a result of various phytochemicals in BACM, these bioactive chemicals also enhanced feed conversion ratio (FCR) in the group. The result obtained is in accordance with the reports of Fascina et al. (2017); Ahsan et al. (2018) when phytogenic additives were fed to broiler chickens. Similar observation was recorded by Dingfa et al. (2017) when turmeric rhizome extract was supplemented of Wenchang broiler chickens. According to Krishnan et al. (2015), intestinal microorganisms play a key role in nutrient absorption and modulating the immune system and metabolic signaling pathways. Hyun et al. (2018); Michiels et al. (2010) and Kim et al. (2013); Shittu et al. (2020) reported that phytogenic feed additives can reduce the activity of pathogenic bacteria by competitive exclusion due to the presence of phenols, alternation of bacteria cells and preventing the development of virulence structures in pathogenic microorganisms. This could be one of the reasons why mortality was not recorded in treatments fed BACM, the test material have also proven its ability to repopulate the beneficial bacteria (Lactobacilli) to maintain dysbiosis. This result is in consonance with the findings of Han et al. (2016); Noohi et al. (2014) and Torok et al. (2011) on the influence of antimicrobial feed additives on broiler commensal post hatch gut microbiota development.
According to Peréz and Aguilar (2013) oxidation occurs during the transfer of electrons from one atom to the other essential for cell metabolism with oxygen as an electron acceptor releasing energy in the form of Adenosine triphosphate. Free radicals are generated during a break in the process predisposing animals to stress and disease but are naturally scavenged from cells by serum antioxidant enzymes i.e., superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) (Hou et al., 2013; Omar, 2013). Phytogenics have been reported to contain antioxidants (phenol and flavonoids) giving total protection to the body and its metabolism against free radicals in the body, thus improving the health status of birds (Olafadehan et al., 2020; Alagbe, 2020). These phytochemicals are also responsible for the rise in serum antibody titres and hormonal immunity especially among birds in T4 and T5. This result in this study is in accordance with the work of Fuluyi and Agbede (2018); Olugbemi et al. (2010).
The use of phytogenic feed additives are one of the ways to ensure food safety, maximize potential and put an end to the leading worrying increase in cases of antibiotic resistance diagnosed in animals and humans through direct contact, environmental contamination and feed consumption. Medicinal plants are of high therapeutic value, relatively cheap, safe and effective. It was concluded from this experiment that BACM can be fed to broiler chicks at 80 ml/liter of water without any deleterious effect on the performance and health status of animals.
Funding: This research received no external funding.
Conflicts of Interest: The authors declare no conflict of interest.
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Dear editorial department: On behalf of our team, I hereby certify the reliability and superiority of the International Journal of Clinical Case Reports and Reviews in the peer review process, editorial support, and journal quality. Firstly, the peer review process of the International Journal of Clinical Case Reports and Reviews is rigorous, fair, transparent, fast, and of high quality. The editorial department invites experts from relevant fields as anonymous reviewers to review all submitted manuscripts. These experts have rich academic backgrounds and experience, and can accurately evaluate the academic quality, originality, and suitability of manuscripts. The editorial department is committed to ensuring the rigor of the peer review process, while also making every effort to ensure a fast review cycle to meet the needs of authors and the academic community. Secondly, the editorial team of the International Journal of Clinical Case Reports and Reviews is composed of a group of senior scholars and professionals with rich experience and professional knowledge in related fields. The editorial department is committed to assisting authors in improving their manuscripts, ensuring their academic accuracy, clarity, and completeness. Editors actively collaborate with authors, providing useful suggestions and feedback to promote the improvement and development of the manuscript. We believe that the support of the editorial department is one of the key factors in ensuring the quality of the journal. Finally, the International Journal of Clinical Case Reports and Reviews is renowned for its high- quality articles and strict academic standards. The editorial department is committed to publishing innovative and academically valuable research results to promote the development and progress of related fields. The International Journal of Clinical Case Reports and Reviews is reasonably priced and ensures excellent service and quality ratio, allowing authors to obtain high-level academic publishing opportunities in an affordable manner. I hereby solemnly declare that the International Journal of Clinical Case Reports and Reviews has a high level of credibility and superiority in terms of peer review process, editorial support, reasonable fees, and journal quality. Sincerely, Rui Tao.
Clinical Cardiology and Cardiovascular Interventions I testity the covering of the peer review process, support from the editorial office, and quality of the journal.
Clinical Cardiology and Cardiovascular Interventions, we deeply appreciate the interest shown in our work and its publication. It has been a true pleasure to collaborate with you. The peer review process, as well as the support provided by the editorial office, have been exceptional, and the quality of the journal is very high, which was a determining factor in our decision to publish with you.
The peer reviewers process is quick and effective, the supports from editorial office is excellent, the quality of journal is high. I would like to collabroate with Internatioanl journal of Clinical Case Reports and Reviews journal clinically in the future time.
Clinical Cardiology and Cardiovascular Interventions, I would like to express my sincerest gratitude for the trust placed in our team for the publication in your journal. It has been a true pleasure to collaborate with you on this project. I am pleased to inform you that both the peer review process and the attention from the editorial coordination have been excellent. Your team has worked with dedication and professionalism to ensure that your publication meets the highest standards of quality. We are confident that this collaboration will result in mutual success, and we are eager to see the fruits of this shared effort.
Dear Dr. Jessica Magne, Editorial Coordinator 0f Clinical Cardiology and Cardiovascular Interventions, I hope this message finds you well. I want to express my utmost gratitude for your excellent work and for the dedication and speed in the publication process of my article titled "Navigating Innovation: Qualitative Insights on Using Technology for Health Education in Acute Coronary Syndrome Patients." I am very satisfied with the peer review process, the support from the editorial office, and the quality of the journal. I hope we can maintain our scientific relationship in the long term.
Dear Monica Gissare, - Editorial Coordinator of Nutrition and Food Processing. ¨My testimony with you is truly professional, with a positive response regarding the follow-up of the article and its review, you took into account my qualities and the importance of the topic¨.
Dear Dr. Jessica Magne, Editorial Coordinator 0f Clinical Cardiology and Cardiovascular Interventions, The review process for the article “The Handling of Anti-aggregants and Anticoagulants in the Oncologic Heart Patient Submitted to Surgery” was extremely rigorous and detailed. From the initial submission to the final acceptance, the editorial team at the “Journal of Clinical Cardiology and Cardiovascular Interventions” demonstrated a high level of professionalism and dedication. The reviewers provided constructive and detailed feedback, which was essential for improving the quality of our work. Communication was always clear and efficient, ensuring that all our questions were promptly addressed. The quality of the “Journal of Clinical Cardiology and Cardiovascular Interventions” is undeniable. It is a peer-reviewed, open-access publication dedicated exclusively to disseminating high-quality research in the field of clinical cardiology and cardiovascular interventions. The journal's impact factor is currently under evaluation, and it is indexed in reputable databases, which further reinforces its credibility and relevance in the scientific field. I highly recommend this journal to researchers looking for a reputable platform to publish their studies.
Dear Editorial Coordinator of the Journal of Nutrition and Food Processing! "I would like to thank the Journal of Nutrition and Food Processing for including and publishing my article. The peer review process was very quick, movement and precise. The Editorial Board has done an extremely conscientious job with much help, valuable comments and advices. I find the journal very valuable from a professional point of view, thank you very much for allowing me to be part of it and I would like to participate in the future!”
Dealing with The Journal of Neurology and Neurological Surgery was very smooth and comprehensive. The office staff took time to address my needs and the response from editors and the office was prompt and fair. I certainly hope to publish with this journal again.Their professionalism is apparent and more than satisfactory. Susan Weiner
My Testimonial Covering as fellowing: Lin-Show Chin. The peer reviewers process is quick and effective, the supports from editorial office is excellent, the quality of journal is high. I would like to collabroate with Internatioanl journal of Clinical Case Reports and Reviews.
My experience publishing in Psychology and Mental Health Care was exceptional. The peer review process was rigorous and constructive, with reviewers providing valuable insights that helped enhance the quality of our work. The editorial team was highly supportive and responsive, making the submission process smooth and efficient. The journal's commitment to high standards and academic rigor makes it a respected platform for quality research. I am grateful for the opportunity to publish in such a reputable journal.
My experience publishing in International Journal of Clinical Case Reports and Reviews was exceptional. I Come forth to Provide a Testimonial Covering the Peer Review Process and the editorial office for the Professional and Impartial Evaluation of the Manuscript.
