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Wound Dressings from Electrospun Gelatin Fibres Coated with Zinc Oxide nano Particles

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Mini Review | DOI: https://doi.org/10.31579/2690-4861/394

Wound Dressings from Electrospun Gelatin Fibres Coated with Zinc Oxide nano Particles

  • N.Gokarneshan *
  • A.Jothimanikandan
  • P.Periyasamy
  • M.Ponmaniselvan
  • K.Sridhar
  • L.D.Kiruthika

Department of Textile Chemistry, SSM College of Engineering, Komarapalayam, Tamil Nadu, India.

*Corresponding Author: N. Gokarneshan, Department of Textile Chemistry, SSM College of Engineering, Komarapalayam, Tamil Nadu, India.

Citation: N.Gokarneshan, A.Jothimanikandan, P.Periyasamy, M.Ponmaniselvan, K.Sridhar, et al, (2024), Wound Dressings from Electrospun Gelatin Fibres Coated with Zinc Oxide nano Particles, International Journal of Clinical Case Reports and Reviews, 17(3); DOI:10.31579/2690-4861/394

Copyright: © 2024, N. Gokarneshan. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Received: 29 January 2024 | Accepted: 15 May 2024 | Published: 24 May 2024

Keywords: gelatin fibers; zno particles; antibacterial activity

Abstract

Traditional wound dressings require frequent replacement, are prone to bacterial growth and cause a lot of environmental pollution. Therefore, biodegradable and antibacterial dressings are eagerly desired. In this paper, gelatin/ZnO fibers were first prepared by side-by-side electrospinning for potential wound dressing materials. The morphology, composition, cytotoxicity and antibacterial activity were characterized by using Fourier transform infrared spectroscopy (FTIR), X-ray diffractometry (XRD), particle size analyzer (DLS), scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDX), thermogravimetry (TGA) and Incucyte™ Zoom system. The results show that ZnO particles are uniformly dispersed on the surface of gelatin fibers and have no cytotoxicity. In addition, the gelatin/ZnO fibers exhibit excellent antibacterial activity against Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) with a significant reduction of bacteria to more than 90%. Therefore, such a biodegradable, nontoxic and antibacterial fiber has excellent application prospects in wound dressing. 

1.Introduction

Skin, as the human body’s largest organ, exerts a vital role in protecting the human body from external harm. Skin damage can lead to microbial invasion of the human body, resulting in a threat to human health [1–3]. Medical dressing is a kind of medical equipment which can cover damaged skin and form a microenvironment conducive to wound healing, thus playing an effective role in wound care and treatment. At present, most medical dressings still use traditional cotton gauze. Nevertheless, traditional cotton gauze needs to be replaced frequently, and easily adheres to the wound, which can easily lead to secondary tissue trauma and bacterial breeding [4–6]. Moreover, huge dressing wastes cause great harm to the environment. Therefore, it is urgent to design new biodegradable and biocompatible dressing materials with good antimicrobial activity. Gelatin, as a kind of natural material, is hydrolyzed from collagen in animals, which has many advantages, such as good accessibility, a wide source of raw materials and low cost. Its amino acid composition is similar to collagen and has good biocompatibility, biodegradability and low immunogenicity [7–9]. Especially in the past decades, with the development of electrospinning technology, gelatin fibers can be prepared simply and quickly. Furthermore, there are many small secondary structures on the surface of gelatin fibers, which is similar to the structure of extracellular matrix (ECM) and closer to the structural size of organisms. Therefore, it plays an important part in cell attachment, growth, migration and differentiation, as well as the formation of new tissues [10–12]. Meanwhile, gelatin fibers have strong adsorption, good filtration, barriers, adhesion and hygroscopicity [13–16]. Therefore, the gelatin 3D nanostructures prepared by electrospinning can be widely used in the biomedical materials, which receives more and more attention. In addition, in recent years nano-inorganic ions and nano-metal oxides have been found to have extensive antibacterial properties, which caused widespread concern [17–19]. Among them, ZnO particles were generally recognized as a safe (GRAS) material by the Food and Drug Administration (FDA) [20]. It has excellent antibacterial properties and minimal effect on human cells, which is extensively used in biomedicine and health products [21–23]. Recently, the blending of ZnO particles with gelatin has been used in the research of antimicrobial materials. Liu et al. prepared gelatin/ethyl cellulose/ZnO nanofibers by electrospinning. The results showed that the nanofibers had a good inhibitory effect on Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) [24]. Chhabra et al. synthesized ZnO doped gelatin and poly-methyl vinyl ether-alt-maleic anhydride (PMVE/MA) composite electrospun scaffolds, which can inhibit bacterial activity and have no cytotoxicity to mammalian cells [25]. Münchow et al. prepared ZnO loaded gelatin/polycaprolactone (PCL) composite nanofibers by electrospinning technique [26]. At present, the current preparation of ZnO/gelatin fibers is usually carried out in the form of blending spinning. Nevertheless, ZnO particles are hard to fully exert efficient antibacterial activity when encapsulated in gelatin fibers. Consequently, it is necessary to increase the content of ZnO particles to achieve better antibacterial effects [27]. However, with that, excess ZnO particles cause waste of materials, and even more seriously excess ZnO particles may lead to cytotoxic effects and affect the growth of the tissue [28–30]. In this paper, unlike the traditional blending method, ZnO particles were dispersed in ethanol, so that ZnO particles can follow the solvent to be sprayed on gelatin fibers by using the side-by-side spray nozzles in the electrospinning process. With the volatilization of ethanol solvent, ZnO particles can be uniform spread only on the surface of gelatin fibers (as shown in Figure 1), so as to achieve the best antibacterial effect with the minimum content of ZnO particles. 

2 Technical details

The following methodology has been adopted

a) Gelatin (type B, basic-processed, prepared by bones, with a molecular weight of 100,000, viscosity value of 4.9 MPa·s −1 ) has been used as the material. 

b) Preparation of ZnO Particles [31-33]

c) Preparation of Gelatin/ZnO Fibers [34-38]

d) Characterization

e) Stability of GZ2 in Aqueous Solutions

f) Cell Culture and Proliferation

g) Antibacterial Evaluation

h) Statistical Analysis

The following studies have been carried out

a) . Morphologies of Gelatin/ZnO Fibers

Characterization of ZnO Particles 

Characterization of Gelatin/ZnO Fibers

b) Bioactivity Studies of Gelatin/ZnO Fibers 

Cell Activity

Antibacterial Activity [39-41]

3 Conclusions

In summary gelatin/ZnO fibers has been first fabricated via side-by-side electrospinning technique as a potential wound dressing. The data indicate that ZnO particles can be uniformly dispersed on the surface of gelatin fibers. Although gelatin/ZnO fibers have some inhibitory effect on MRC-5 cells, the corresponding cytotoxicity level is 0 or 1 per the standard, which indicates that fibers have no cytotoxicity. Moreover, the gelatin/ZnO fibers show excellent antibacterial activity against E. coli and S. aureus, which is caused by the superoxide radicals (·O2 −) produced by ZnO particles. In addition, the reductions of bacteria are all more than 90%. The experiments imply that gelatin/ZnO fibers have excellent application prospects for wound dressing.

References

  1. Liang, D.; Lu, Z.; Yang, H.; Gao, J.; Chen, R. (2016). A novel asymmetric wettable agnps/chitosan wound dressing: In vitro and in vivo evaluation. ACS Appl. Mater. Interfaces, 8, 3958–3968.
    View at Publisher | View at Google Scholar
  2. Pei, Y.; Ye, D.; Zhao, Q.; Wang, X.; Zhang, C.; Huang, W.; Zhang, N.; Liu, S.; Zhang, L. (2015). Effectively promoting wound healing with cellulose/gelatin sponges constructed directly from a cellulose solution. J. Mater. Chem. B, 3, 7518–7528.
    View at Publisher | View at Google Scholar
  3. Wang, Y.; Dou, C.; He, G.; Ban, L.; Huang, L.; Li, Z.; Gong, J.; Zhang, J.; Yu, P. (2018). Biomedical Potential of Ultrafine Ag particles Coated on Poly (Gamma-Glutamic Acid) Hydrogel with Special Reference to Wound Healing. Nanomaterials, 8, 324.
    View at Publisher | View at Google Scholar
  4. Guo, Y.; Pan, S.; Jiang, F.; Wang, E.; Miinea, L.; Marchant, N.; Cakmak, M. (2018). Anisotropic swelling wound dressings with vertically aligned water absorptive particles. RSC Adv., 8, 8173–8180.
    View at Publisher | View at Google Scholar
  5. Zhao, J.; Qu, Y.; Chen, H.; Xu, R.; Yu, Q.; Yang, P. (2018). Self-assembled proteinaceous wound dressings attenuate secondary trauma and improve wound healing in vivo. J. Mater. Chem B, 6, 4645–4655.
    View at Publisher | View at Google Scholar
  6. Farokhi, M.; Mottaghitalab, F.; Fatahi, Y.; Khademhosseini, A.; Kaplan, D.L. (2018). Overview of Silk Fibroin Use in Wound Dressings. Trends Biotechnol. , 36, 907–922.
    View at Publisher | View at Google Scholar
  7. Aldana, A.A.; Malatto, L.; Rehman, M.A.U.; Boccaccini, A.R.; Abraham, G.A. (2019). Fabrication of Gelatin Methacrylate (GelMA) Scaffolds with Nano- and Micro-Topographical and Morphological Features. Nanomaterials , 9, 120.
    View at Publisher | View at Google Scholar
  8. Chen, Y.; Lu, W.; Guo, Y.; Zhu, Y.; Lu, H.; Wu, Y. (2018). Superhydrophobic coatings on gelatin-based films: Fabrication, characterization and cytotoxicity studies. RSC Adv. , 8, 23712–23719.
    View at Publisher | View at Google Scholar
  9. Ou, Q.; Miao, Y.; Yang, F.; Lin, X.; Zhang, L.-M.; Wang, Y. (2019). Zein/gelatin/nanohydroxyapatite nanofibrous scaffolds are biocompatible and promote osteogenic differentiation of human periodontal ligament stem cells. Biomater. Sci.
    View at Publisher | View at Google Scholar
  10. Kuppan, P.; Sethuraman, S.; Krishnan, U.M. (2016). Fabrication and investigation of nanofibrous matrices as esophageal tissue scaffolds using human non-keratinized, stratified, squamous epithelial cells. RSC Adv., 6, 26461–26473.
    View at Publisher | View at Google Scholar
  11. Sahay, R.; Kumar, P.S.; Sridhar, R.; Sundaramurthy, J.; Venugopal, J.; Mhaisalkar, S.G.; (2016). Ramakrishna, S. Electrospun composite fibers and their multifaceted applications. J. Mater. Chem. , 22, 12953–12971.
    View at Publisher | View at Google Scholar
  12. Lei, B.; Shin, K.-H.; Noh, D.-Y.; Jo, I.-H.; Koh, Y.-H.; Choi, W.-Y.; Kim, H.-E. (2012). Nanofibrous gelatin-silica hybrid scaffolds mimicking the native extracellular matrix (ECM) using thermally induced phase separation. J. Mater. Chem., 22, 14133–14140. Nanomaterials 2019, 9, 525 12 of 13
    View at Publisher | View at Google Scholar
  13. Liu, Y.; Deng, L.; Zhang, C.; Feng, F.; Zhang, H. (2018). Tunable Physical Properties of Ethylcellulose/Gelatin Composite fibers by Electrospinning. J. Agric. Food Chem 66, 1907–1915.
    View at Publisher | View at Google Scholar
  14. Ren, X.; Feng, Y.; Guo, J.; Wang, H.; Li, Q.; Yang, J.; Hao, X.; Lv, J.; Ma, N.; Li, W. (2015). Surface modification and endothelialization of biomaterials as potential scaffolds for vascular tissue engineering applications. Chem. Soc. Rev., 44, 5680–5742.
    View at Publisher | View at Google Scholar
  15. Jalaja, K.; Sreehari, V.S.; Kumar, P.R.A.; Nirmala, R.J. (2015). Graphene oxide decorated electrospun gelatin fibers: Fabrication, properties and applications. Mater. Sci. Eng. C, 64, 11–19.
    View at Publisher | View at Google Scholar
  16. Li, H.; Wang, M.; Williams, G.R.; Wu, J.; Sun, X.; Lv, Y.; Zhu, L.-M. (2016). Electrospun gelatin fibers loaded with vitamins A and E as antibacterial wound dressing materials. RSC Adv. 6, 50267–50277.
    View at Publisher | View at Google Scholar
  17. Perelshtein, I.; Applerot, G.; Perkas, N.; Wehrschetz-Sigl, E.; Hasmann, A.; Guebitz, G.M.; Gedanken, A. (2008). Antibacterial Properties of an In Situ Generated and Simultaneously Deposited Nanocrystalline ZnO on Fabrics. ACS Appl. Mater. Interfaces, 1, 361–366.
    View at Publisher | View at Google Scholar
  18. Manna, J.; Begum, G.; Kumar, K.P.; Misra, S.; Rana, R.K. (2013). Enabling Antibacterial Coating via Bioinspired Mineralization of Nanostructured ZnO on Fabrics under Mild Conditions. ACS Appl. Mater. Interfaces, 5, 4457–4463.
    View at Publisher | View at Google Scholar
  19. Dutta, R.K.; Sharma, P.K.; Bhargava, R.; Kumar, N.; Pandey, A.C. (2010). Differential Susceptibility of Escherichia coli Cells toward Transition Metal-Doped and Matrix-Embedded ZnO particles. J. Phys. Chem. B , 114, 5594–5599.
    View at Publisher | View at Google Scholar
  20. Vijayakumar, S.; Vaseeharan, B. (2018).Antibiofilm, anti cancer and ecotoxicity properties of collagen based ZnO particles. Adv. Powder Technol., 29, 2331–2345.
    View at Publisher | View at Google Scholar
  21. Huang, Z.; Zheng, X.; Yan, D.; Yin, G.; Liao, X.; Kang, Y.; Yao, Y.; Huang, D.; Hao, B. (2008).Toxicological Effect of ZnO particles Based on Bacteria. Langmuir , 24, 4140–4144.
    View at Publisher | View at Google Scholar
  22. Brayner, R.; Dahoumane, S.A.; Yéprémian, C.; Djediat, C.; Meyer, M.; Couté, A.; Fiévet, F. ZnO (2010). particles: Synthesis, Characterization, and Ecotoxicological Studies. Langmuir, 26, 6522–6528.
    View at Publisher | View at Google Scholar
  23. Chen, Y.; Ding, H.; Sun, S. Preparation and Characterization of ZnO particles Supported on Amorphous SiO2 . Nanomaterials 2017, 7, 217.
    View at Publisher | View at Google Scholar
  24. Liu, Y.; Li, Y.; Deng, L.; Zou, L.; Feng, F.; Zhang, H. (2018). Hydrophobic Ethylcellulose/Gelatin fibers Containing Zinc Oxide particles for Antimicrobial Packaging. J. Agric. Food Chem., 66, 9498–9506.
    View at Publisher | View at Google Scholar
  25. Chhabra, H.; Deshpande, R.; Kanitkar, M.; Jaiswal, A.; Kale, V.P.; Bellare, J.R. A (2016). nano zinc oxide doped electrospun scaffold improves wound healing in a rodent model. RSC Adv. , 6, 1428–1439.
    View at Publisher | View at Google Scholar
  26. Münchow, E.A.; Albuquerque, M.T.P.; Zero, B.; Kamocki, K.; Piva, E.; Gregory, R.L.; Bottino, M.C. (2015). Development and characterization of novel ZnO-loaded electrospun membranes for periodontal regeneration. Dent. Mater., 31, 1038–1051.
    View at Publisher | View at Google Scholar
  27. Sudheesh Kumar, P.T.; Lakshmanan, V.-K.; Anilkumar, T.V.; Ramya, C.; Reshmi, P.; Unnikrishnan, A.G.; Nair, S.V.; Jayakumar, R. (2012). Flexible and Microporous Chitosan Hydrogel/Nano ZnO Composite Bandages for Wound Dressing: In Vitro and In Vivo Evaluation. ACS Appl. Mater. Interfaces, 4, 2618–2629.
    View at Publisher | View at Google Scholar
  28. Luo, M.; Shen, C.; Feltis, B.N.; Martin, L.L.; Hughes, A.E.; Wright, P.F.A.; Turney, T.W. (2014). Reducing ZnO particle cytotoxicity by surface modification. Nanoscale , 6, 5791.
    View at Publisher | View at Google Scholar
  29. Hsiao, I.-L.; Huang, Y.-J. (2011). Titanium Oxide Shell Coatings Decrease the Cytotoxicity of ZnO particles. Chem. Res. Toxicol., 24, 303–313.
    View at Publisher | View at Google Scholar
  30. Hong, T.-K.; Tripathy, N.; Son, H.-J.; Ha, K.-T.; Jeong, H.-S.; Hahn, Y.-B. A (2013). comprehensive in vitro and in vivo study of ZnO particles toxicity. J. Mater. Chem. B, 1, 2985–2992.
    View at Publisher | View at Google Scholar
  31. Zhang, Y.; Chung, J.; Lee, J.; Myoung, J.; Lim, S. (2011). Synthesis of ZnO nanospheres with uniform nanopores by a hydrothermal process. J. Phys. Chem. Solids , 72, 1548–1553. 32.
    View at Publisher | View at Google Scholar
  32. Xiong, G.; Pal, U.; Serrano, J.G.; Ucer, K.B.; Williams, R.T. (2010). Photoluminesence and ftir study of ZnO particles: The impurity and defect perspective. Phys. Status Solidi , 3, 3577–3581.
    View at Publisher | View at Google Scholar
  33. Ran, J.; He, M.; Li, W.; Cheng, D.; Wang, X. Growing ZnO (2018). particles on polydopamine-templated cotton fabrics for durable antimicrobial activity and UV protection. Polymers, 10, 495.
    View at Publisher | View at Google Scholar
  34. Chen, Y.; Ma, Y.; Lu, W.; Guo, Y.; Zhu, Y.; Lu, H.; Song, Y. (2018). Environmentally Friendly Gelatin/β-Cyclodextrin Composite Fiber Adsorbents for the Efficient Removal of Dyes from Wastewater. Molecules , 23, 2473.
    View at Publisher | View at Google Scholar
  35. Gupta, N.; Santhiya, D. (2019). In situ mineralization of bioactive glass in gelatin matrix. Mater. Lett. 2017, 188, 127–129. [CrossRef] Nanomaterials , 9, 525 13 of 13
    View at Publisher | View at Google Scholar
  36. Lomakin, S.M.; Rogovina, S.Z.; Grachev, A.V.; Prut, E.V.; Alexanyan, C.V. (2011). Thermal Degradation of Biodegradable Blends of Polyethylene with Cellulose and Ethylcellulose. Thermochim. Acta, 521, 66–73.
    View at Publisher | View at Google Scholar
  37. Kwak, H.W.; Kim, J.E.; Lee, K.H. (2019). Green fabrication of antibacterial gelatin fiber for biomedical application. React. Funct. Polym. , 136, 86–94.
    View at Publisher | View at Google Scholar
  38. Lonkar, S.P.; Pillai, V.; Abdala, A. (2019).Solvent-free Synthesis of ZnO-Graphene Nanocomposite with Superior Photocatalytic Activity. Appl. Surf. Sci. , 465, 1107–1113. 39.
    View at Publisher | View at Google Scholar
  39. Patil, P.P.; Meshram, J.V.; Bohara, R.A.; Nanaware, S.G.; Pawar, S.H. ZnO (2018). particle-embedded silk fibroin-polyvinyl alcohol composite film: A potential dressing material for infected wounds. New J. Chem., 42, 14620–14629.
    View at Publisher | View at Google Scholar
  40. Bai, X.; Li, L.; Liu, H.; Tan, L.; Liu, T.; Meng, X. Solvothermal Synthesis of ZnO (2015). particles and Anti-Infection Application in Vivo. ACS Appl. Mater. Interfaces, 7, 1308–1317.
    View at Publisher | View at Google Scholar
  41. Raghupathi, K.R.; Koodali, R.T.; Manna, A.C. (2011). Size-Dependent Bacterial Growth Inhibition and Mechanism of Antibacterial Activity of Zinc Oxide particles. Langmuir, 27, 4020–4028.
    View at Publisher | View at Google Scholar
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