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Review Article | DOI: https://doi.org/10.31579/2690-8808/217
1Department of Eastern Medicine, Government College University Faisalabad-Pakistan
2Faculty of Medical Sciences, Government College University Faisalabad-Pakistan
3DISAE Research Group. University of La Laguna. Spain
4Molecular Nutritional and Biochemistry Laboratory, University of Peradeniya, Sri Lanka
5Department of Cell Biology, Center of Research and Advanced Studies of the National Polytechnical Institute, Mexico City, Mexico.
6Department of Pharmacology and Toxicology, Faculty of Pharmacy, Umm Al Qura University, Makkah, Saudi Arabia
7Department of Molecular Biology and Genetics, Faculty of Science and Art, Bingol University, Bingol, 1200, Türkiye
8Department of Pharmacology and Clinical Pharmacy, Universitas Andalas, Indonesia
9Department of Neurology, Neuroceuta, (Virgen de Africa Clinic), Spain
10Department of Psychiatry, Patton State Hospital, USA
11Department of Pharmacy, University of Pisa, Via Bonanno, 6, I-56126 Pisa, Italy
12Faculty of Health and Life Sciences, INTI International University, 71800, Putra Nilai, Negeri Sembilan, Malaysia.
13Department of Sociology & Criminology, University of Sargodha, Sargodha
147BCNet International Working Group, IARC/WHO, Dakar –Senegal
15Department of Medical Laboratory Science, Faculty of Allied Health Sciences, Bayero University, Kano, Nigeria
16Pharmacy, Bangabandhu Sheikh Mujibur Rahman Science and Technology University, Bangladesh
17Federal College of Forest Resources Management Maiduguri Borno State Nigeria
*Corresponding Author: Muhammad Akram, Department of Eastern Medicine, Government College University Faisalabad-Pakistan.
Citation: Muhammad Akram, Abid Rashid, David P. Jorge, Fahad S. Khan, Shafiq Ur Rehman, et al, (2024), Single-Cell Genomic DNA Profiling of Microbial Species in Fungal Hosts, J, Clinical Case Reports and Studies, 5(7); DOI:10.31579/2690-8808/217
Copyright: ©, 2024, Muhammad Akram. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Received: 27 August 2024 | Accepted: 09 September 2024 | Published: 17 September 2024
Keywords: genomic; DNA sequencing; microbial species; single cells; fungus
This study presents a novel approach to understanding microbial differing qualities inside contagious has through single-cell genomic DNA profiling. By confining and sequencing single cells, we capture the genomic scenes of fungal-resident microbial species, advertising uncommon determination of their hereditary cosmetics. This strategy overcomes the restrictions of enormous sequencing, uncovering perplexing microbial intelligent and already veiled varieties. Our discoveries give experiences into microbial community structure, utilitarian possibilities, and developmental elements inside contagious environments. This approach not as it were progresses microbial genomics but moreover makes strides our understanding of host-microbe connections in complex situations.
The globe is making efforts to eradicate infectious parasites, unlike other organisms such as plants, because parasites are the second most diverse group of species after arthropods [5]. As microbial decomposers and nutrient exchangers, these organisms are vital to ecosystems [13]. However, the mechanisms of their individual development in nature are still largely unknown [4]. The spread of harmful and prevalent parasitic diseases poses a significant threat to food security, with various illnesses, including oomycetes, leading to some of the most unexpected wildlife extinctions recorded [15]. The frequencies of harmful microbes have been observed to be significant [2]. Documenting every type of parasite can assist in recognizing, managing, and preventing wildlife failures caused by microbial infections [6]. With 1.5–3 million species reported as extinct globally, the projection is that all plant species, animals, and microorganisms will be represented between 30 and 90 years old, and completely represented parasites were previously estimated to make up only 7% of the 1.5 million species [3]. Between 1980–1989, 1,229 species were reviewed annually, with 1,097 between 1990–1999, and 1,196 between 1999–2009 [7] The estimated number of parasitic species worldwide has increased from 2.2 to 3.8 million, with estimates reaching up to 5.1 million [16]. This estimate is based on observed growth in parasitic species and their ecological impacts [8]. Phylogenetic studies using DNA markers have been instrumental in identifying diverse parasitic species and understanding their evolutionary history [10]. For example, the C. gloeosporioides complex includes multiple species interacting with various plant hosts [12]. Research also focuses on single-cell genome techniques to study parasites like Caulochytrium protostelioides, Dimargaris cristalligena, Piptocephalus cylindrospora, and Rozella allomycis [17]. These techniques help understand the genetic makeup and evolutionary dynamics of these organisms [18]. Accurate identification and understanding of these parasites are essential for addressing their ecological impact and developing effective management strategies [22].
Techniques for single-cell analysis have developed throughout time, and they are currently mostly employed in basic and applied research. They come from samples taken in the environment. We are presently refining the MDA reaction's enzymology to eliminate bias and chimeric rearrangements. Notwithstanding these drawbacks, single-cell sequencing should enable rapid progress in determining metabolic traits and environmental adaptations in the vast majority of uncultivated microorganisms. It also provides a new method for researching genetic variation patterns within and between species in phylogenetic, epidemiological, and evolutionary research.
Single-cell genomic DNA profiling uncovers point by point hereditary differing qualities of microbial species inside contagious has, advertising more profound experiences into microbial intuitive and community structure. This strategy makes strides our understanding of have organism elements and gives a effective device to investigate complex microbial biological systems with phenomenal determination. Sequencing the genomic DNA of microbial species from individual fungal cells is one of the most sophisticated techniques for determining the genetic composition of individual cells within a fungal community. Our approach illuminates features of the genetic diversity and distinctive characteristics of fungi that large-scale sequencing approaches could miss. Recent advancements have made it possible to sequence genomic DNA from individual microbial cells. Utilizing every laboratory method to its fullest potential is the main objective of this study's design. Genome sequencing is made possible by single-cell sequencing, which eliminates the need for developed organisms and their genome sequencing as a prerequisite. Multiple displacement amplification (MDA) can be used to obtain an increased copy number of a single bacterium. During the DNA amplification process, many sequence rearrangements may occur. According to several research articles, during the past two years, many organizations have been employing MDA and creating practical techniques for sequencing individual cells. Newer studies show that consensus is developing on best practices, amplified template trustworthiness, "composite" genomes, and how to properly evaluate them. To finish the assembly, data from several single-cell MDA reactions are combined to form the basis of the study.
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