Post-COVID-19 Aortitis with Acute Thoracic Aortic Aneurysms: A Gene Expression and Molecular Pathway Analysis

Research Article | DOI: https://doi.org/10.31579/2690-1897/199

Post-COVID-19 Aortitis with Acute Thoracic Aortic Aneurysms: A Gene Expression and Molecular Pathway Analysis

  • Michael P. Dorsey
  • Navneet Narula
  • Harvey I. Pass, Les James
  • Valeria Mezzano
  • Hua Zhou
  • Aristotelis Tsirigos
  • Sitharam Ramaswami
  • Mark D. Peterson
  • Deane E. Smith
  • Aubrey C. Galloway *

NYU Grossman School of Medicine, New York, NY 10010 

*Corresponding Author: Aubrey C. Galloway, MD Seymour Cohn Professor Department of Cardiothoracic Surgery NYU Grossman School of Medicine 530 1st Avenue, Suite 9V, New York, NY 10016.

Citation: Michael P. Dorsey, Navneet Narula, Harvey I. Pass, Les James, Valeria Mezzano, et al, (2024), post-COVID-19 Aortitis with Acute Thoracic Aortic Aneurysms: A Gene Expression and Molecular Pathway Analysis, J, Surgical Case Reports and Images, 7(7); DOI:10.31579/2690-1897/199

Copyright: © 2024, Aubrey C. Galloway. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Received: 14 June 2024 | Accepted: 03 July 2024 | Published: 14 August 2024

Keywords: digital spatial profiling, covid19, inflammatory thoracic aortic aneurysms

Abstract

Post-COVID-19 aortitis (AoC19) with aneurysm formation has been described, but little is    known about the molecular pathophysiology. This study utilized Digital Spatial Profiling (DSP) to evaluate differentially expressed genes (DEG) and molecular pathway activation in the aorta of AoC19 compared to Giant Cell aortitis (AoGC). 

Methods:  Histopathology analysis was performed on AoC19 and AoGC and regions of interest (ROI) based on varying degrees of inflammation were chosen and encircled. ROIs were stained with fluorescent anti-cytokeratin, anti-leukocyte, anti-nuclear antibodies and profiling agents for 1,850 RNA targets using the COVID-19 Immune Response Atlas. DSP was performed to compare DEG in AoC19 compared to AoGC, and across zones of AoC19 itself. Molecular pathway activation and gene primary ontology functions were analyzed. 

Results: 103 DGEs were demonstrated when comparing AoC19 to AoGC. In AoC19, the pathways activated were associated with viral protein interaction with cytokines and cytokine receptors, cytokine-cytokine receptor interactions, and COVID-19; while in AoGC, pathways activated were associated with human leukocyte antigens HLA-DRB1, HLA-DRB4, and CD4 T cells. 42 DGEs were demonstrated when comparing ROIs across AoC19 itself, activating molecular pathways associated with cytokine-cytokine receptor interactions, complement and coagulation cascades, signaling pathways for TNF, IL-17 and nuclear factor-kappa ß, and rheumatoid arthritis. The primary molecular ontology functions of the DGEs across AoC19 were for COVID-19, Kawasaki Disease/Takayasu arteritis, and aneurysm formation.

Conclusions:  This study demonstrated differential gene expression in the aortic wall of AoC19 with the activation of molecular pathways shared between COVID-19, Kawasaki Disease/Takayasu arteritis and aneurysm formation. 

Glossary of Abbreviations

AoC19                    aortic aneurysm specimen from patient with post-COVID-19 aortitis

AoGC                     aortic aneurysm specimen from patient with Giant Cell aortitis 

COVID-19             coronavirus 2019 disease

DEG                       differentially expressed genes 

DSP                        digital spatial profiling

FDR                        false discovery rate

KEGG                    Kyoto Encyclopedia of Genes and Genomes 

ROI                        regions of interest

Graphical Abstract

Introduction

Patients with COVID-19 can develop serious cardiovascular complications resulting in  increased morbidity and mortality, including acute myocardial infarction, myocarditis, vasculitis, vascular thrombosis and thromboembolic events.[1,2] The  SARS-CoV-2  viral spike (S) protein enters cells through angiotensin-converting enzyme 2  (ACE2) receptors, which  are highly expressed in the heart, vascular system, respiratory tract and gastrointestinal tract.  RNA sequencing data from multiple vascular beds, however, has demonstrated a  low level of ACE2 expression in vascular endothelial cells compared to epithelial cells in the respiratory track and enterocytes in the gastrointestinal tract.[3-6] Therefore,  much of the vascular endothelial cell damage in COVID-19 may be mediated by the host inflammatory immune response through  the activation of inflammatory cytokines, macrophage and lymphocyte infiltration, and platelet and complement activation.  The SARS-CoV-2 viral spike (S) protein also binds directly to toll-like receptors and nucleotide-binding and oligomerization domain (NOD)-like receptors on host leukocytes to activate the host immune system directly. In the most severely ill COVID-19 patients the host immune response was found to be  dysregulated and maladaptive, which contributed to multisystem organ damage and the high mortality in these patients.[7] The host immune transcriptomic response signature to COVID-19 can persist for 6 weeks to 8 months after the initial infection and,   result in late post-acute COVID-19 sequelae in certain patients.8,9  Late sequelae most commonly occurs  2-3 months after the initial infection, and the etiology is thought to be due to  a persistent, maladaptive  host immune response pattern rather than ongoing infection.[9]  The development post COVID-19 aortic aneurysms has been reported as a rare late complication,  although the molecular mechanism is unknown. [10,11,12] 

This study was generated after we treated a patient who presented with large vessel aortitis and acute thoracic aortic aneurysms six weeks after a mild COVID-19 respiratory illness.  Based on the temporal relationship to the initial COVID-19 infection, and the unusual operative findings of acute inflammatory thoracic aortic aneurysms, with areas localized dissection, acute thrombus, and multiple pseudoaneurysms, our hypothesis was that this likely represented a late post-acute COVID-19 sequelae of large vessel inflammatory aortitis (AoC19). This study was designed to evaluate gene expressing and the molecular pathway activating patterns in the aortic wall of this patient (AoC19) using Digital Spatial Profiling (DSP) and to compare this to patient with Giant Cell aortitis (AoGC). 

Materials and Methods

Patient Population 

A 59-year-old female with a history of hypertension, diabetes, obesity, and metabolic syndrome presented with mild upper respiratory symptoms, normal oxygen saturation scattered mild infiltrates on Chest X-ray, a normal mediastinum, and tested positive for COVID-19 on nasopharyngeal swab. She did not require oxygen or hospital admission and was discharged. At home she remained clinically well, without further symptom, and apparently recovered, but developed left chest pain and back pain 6 weeks later and represented to the emergency room. The follow-up chest x-ray revealed clearing of the prior pulmonary infiltrates, but demonstrated a newly enlarged mediastinum. The nasopharyngeal swab for COVID-19 was negative, while blood work demonstrated anti-COVID-19 immunoglobulins (IgG.3.4, IgG 16.9, and IgM 3.9) consistent with a prior COVID-19 infection, and elevated inflammatory markers (D-dimer 1258 ng/mL, Ferritin 1035 ng/mL, and C-reactive protein 220 mg/L). Work up for infection (blood cultures, human immunodeficiency virus) and autoimmune disease (antinuclear antibodies, rapid plasma reagin, and antiphospholipid antibodies) was enegative. A computed tomographic angiography (CTA) of the chest demonstrated diffuse thickening of the thoracic aorta consistent with aortitis, and saccular pseudo-aneurysms in both the ascending and descending thoracic aorta. (Figure 1) Due to ongoing left lower back pain she underwent urgent endovascular aortic repair (TEVAR) of the descending thoracic aorta.  Her pain resolved and she was discharged home on a course of steroids for presumed inflammatory aortitis, with plans for follow-up elective open repair of the ascending aorta and aortic arch in 2-3 weeks.  However, she developed recurrent chest pain in 2 weeks and was readmitted for urgent surgery for ascending aorta and zone 2 aortic arch replacement. The operative findings included extensive inflammation and thickening of the aorta, with multiple areas of localized dissection, thrombus, and pseudoaneurysms. (Figure 2, B, C) She recovered uneventfully and was discharged home on postoperative day 5. The postoperative CT scan demonstrating the ascending aorta and zone II aortic arch replacement is shown in Figure 3.

 

Figure 1. CTA coronal view with arrow demonstrating 4.5cm saccular pseudoaneurysm of the ascending aorta CTA coronal view with arrows demonstrating two descending thoracic aortic pseudoaneurysm (arrows) CTA three-dimensional reconstruction demonstrating multiple thoracic aortic pseudoaneurysms.

CTA, computed tomographic angiography

 

Figure 2. A. Completion aortogram after initial endovascular repair of the descending thoracic aorta. B. Intraoperative image demonstrating the whitish, inflamed and thickened ascending aorta. C. Intraoperative image demonstrating intimal thickening and multiple penetrating defects in the inner curvature of the open aortic arch.

Figure 3. Postoperative CT scan demonstrating the ascending aorta and zone II aortic arch repair with debranching, and prior descending thoracic aorta stent graft.  CT (computed tomographic).

Methods

This study was conducted under the NYU Grossman School of Medicine Lung Cancer Biomarker Center (#I8896) and underwent expedited IRB review and approval in 2021.  Histopathologic analysis, along with special bacterial and fungal stains, was performed on the AoC19  specimen, and on a specimen  from a patient with Giant Cell aortitis (AoGC) who had previously undergone ascending aneurysm repair.  Electron microscopy to test for viral particles was also performed on the AoC19 specimen. Regions of interest (ROI) in different zones of the aortic, based on varying degrees of inflammation or fibrosis, and were marked and encircled for subsequent analysis. Formalin-fixed, paraffin-embedded sections of the specimens were subjected to a standard antigen retrieval protocol and stained with fluorescent anti-cytokeratin (i.e. pan-cytokeratin), anti-leukocyte (i.e. CD45) antibodies, and DNA binding SYTO-13 for nuclear recognition. The barcodes for each fluorescent DNA target in the ROIs were then selected for analysis and decoded for sequencing. (Figure 4) DSP) was then performed to evaluate DEGs, using the COVID-19 Immune Response Atlas (NanoString, WA, USA), which included a combination of the GeoMx Cancer Transcriptome Atlas of 1850 immune genes with an additional 30 genes from a specially designed COVID-19 panel.  DEG analyses were performed by comparing ROI’s in AoC19 to AoGC, and by comparing ROIs across various zones across 

AoC19 itself, representing normal areas, and  zones with varying  degrees of inflammation or fibrosis. Log fold changes with a false discovery rate (FDR) of <0>

Results

Histopathology

Histopathologic analysis of AoC19 demonstrated patchy necrosis of the aortic wall, with localized areas of dissection, thrombus, and multiple sterile abscesses. Stains for fungal and bacterial organisms were negative, and no viral particles were identified in the AoC19 specimen by electron microscopy.  Immunofluorescence microscopy of AoC19 demonstrated acute thrombus with infiltrating neutrophils, with surrounding areas of lymphoplasmacytic inflammation, and areas of fibrosis in the adventitia and in AoGC demonstrated histiocytic inflammation, with granulomatous aortitis and giant cells, as is typically seen in AoGC.   ROIs of each specimen, based on normal appearing areas and areas with varying degrees of inflammation or fibrosis, were encircled for subsequent analysis by DSP. (Figure 5, sidebars)

Figure 5. Digital Spatial transcriptomic heat-map identifying differentially expressed genes when comparing post-COVID-19 aortitis (AoC19) to giant cell arteritis (AoGC). Histologic sections of each =specimen are demonstrated on the side panels, with the colored squares in the heat map corresponding to the regions of interest (ROI)). The black line separates the AoGC clustes (left) from the AoC19 clusters (right). At the bottom of the heat map the ROI squares corresponding to the varying degrees of progressive inflammation across AoC19 itself are arranged from left to right.

Digital Spatial Profiling and KEGG Pathway Analysis

In comparing AoC19 to AoGC, 103 DGEs were demonstrated. The DEGs in AoC19 activated KEGG pathways associated with viral protein interaction with cytokines and cytokine receptors, cytokine-cytokine receptor interactions, and COVID-19; while in AoGC the KEGG pathways activated were associated with human leukocyte antigens HLA-DRB1, HLA-DRB4, and CD4 T cells. 42 DGEs were demonstrated when comparing ROIs across various inflammatory zones of AoC19 itself, (Table 1) activating KEGG pathways associated with cytokine-cytokine receptor interactions (q value=0.002); complement and coagulation cascades (q value=0.0009); signaling pathways for TNF (q value=0.004), IL-17 (q value=0.009), and NF-kß (q value=0.013); and rheumatoid arthritis (q value=0.009);  Citrullinated H3-5, CLEC5A and CXCL2  are associated with neutrophil extracellular trap  formation, neutrophil and macrophage migration, Kawasaki Disease and COVID-19.[15,16], The primary ontology functions of the DEGs identified across the  zones of AoC19 were for COVID-19 infection (purple font), Kawasaki Disease/Takayasu arteritis (green font), and aneurysm formation (red font). (Figure 6)

GeneLog Fold ChangeFDR
ITGA8-1.3720.012
CES3-0.9390.013
CFI-0.6110.022
ACTA2-1.5410.034
IL22-0.6460.035
USP9Y-0.8990.035
TNFRSF11B-1.6750.036
ARG2-0.7600.040
ITGAX1.7240.000
H3-3A1.7020.001
AQP91.1300.002
CXCR41.7140.006
KRT140.8720.008
CD550.8790.010
RPS27A1.5050.012
CSF3R1.6890.012
VEGFA2.3890.012
SIRPA0.9420.012
IER31.8980.012
RPL7A1.3740.013
TREM11.1630.015
CXCL20.9880.015
SGK10.9840.015
ALDOA1.6010.015
TNFAIP60.8570.015
TNFAIP31.2000.018
ICAM11.0170.022
SERPINA12.3460.023
IL1R21.0180.030
TNFRSF1B0.7300.030
PTPN110.6940.030
MMP12.6770.032
BMP21.4210.032
CD441.7340.033
CEBPB0.9080.035
NFKB20.6390.035
DDIT41.0500.035
CLEC5A1.0530.038
CD531.0350.040
H3-51.0750.040
FPR10.8350.043
VSIR0.6230.050

Table 1. The significant differentially expressed genes across zones of AoC19 itself when comparing normal  zones to  zones with varying degrees of inflammation or fibrosis.

Figure 6: The digital spatial transcriptomic heat-map that displays the 42 significant differentially expressed genes (DEGs) across the various inflammatory zones of AoC19 along the vertical y-axis, and displays the zones of AoC19 with increasing degrees of inflammation or fibrosis from left to right along the horizontal x-axis. The primary ontology functions of the DEGs were for COVID-19 (purple font), Kawasaki Disease/Takayasu arteritis (green font), and aneurysm formation (red font).

Discussion

The host inflammatory process plays a major role in the development of thoracic aortic aneurysms, producing endothelial cell dysfunction, vascular smooth muscle cell stress with a change in phenotype, and the  activation of matrix metalloproteases in the extracellular matrix. (13)  An analysis of bulk transcriptomome and single-cell RNA sequencing data identified 1525 DEGs in thoracic aortic aneurysms compared to normal aortic controls, activating multiple immune cell types, including macrophages, neutrophils, and T cells, and molecular pathways involved with leukocyte trans-endothelial migration, and the activation of complement and coagulation cascades. Thirty-nine (39) DEGs were identified in the macrophage population of and 30 DEGs in the T cell population. These were associated with increased infiltration of M2 macrophages and CD8+ T cells into the aortic wall, suggesting a major role of these cell types in development of thoracic aneurysms. (13) 

Large vessel vasculitis with inflammatory thoracic aneurysms are most commonly due to  Giant Cell aortitis (AoGC),  Takayasu’s arteritis, and isolated aortitis. (14,15)  While the exact etiology is unknown, the molecular pathophysiology is thought to be antigen related, with the activation of inflammatory cytokines, macrophages, and T cells (13) Using global transcriptomic profiling and bulk RNA sequencing, Hur and associates compared DEG signatures between inflammatory and non-inflammatory aneurysms, and demonstrated 159 upregulated DEGs and 93 downregulated DEGs in the inflammatory  group. (16) Gene ontology enrichment demonstrated that the top 3 gene ontology terms were for immune response, host defense response, and inflammatory response;  and pathways associated with increased cytokine and chemokine activity, and autoimmune disease. (16). Patients with Giant Cell aortitis have been shown to have DEGs in the aortic wall and circulating leukocytes that activate CD4 T cells and macrophages, and molecular pathways associated with a Type I interferon signature.[17]

Multisystem inflammatory syndrome in children (MIS-C) is a systemic medium vessel vasculitis thought to be triggered by SARS-CoV-2. It typically develops 3-4 weeks after the initial infection, (18,19,20)   MIS-C) and is distinct from Kawasaki Disease, but with overlapping transcriptomic pathways.  (18). An artificial intelligence-guided review of host immune signatures in the COVID-19 pandemic revealed shared host immune response patterns between COVID-19 (SARS-Co-V-2), MIS-C, and Kawasaki disease.  (20). 

The patient in this study presented 6 weeks after a mild COVID-19 infection with acute large vessel vasculitis and thoracic aortic aneurysms. There were no viral particles in the aortic specimen and the patient did not have an active infection.  Our findings, however, demonstrated DEGs in the aortic wall that activated KEGG pathways associated with viral protein interaction with cytokines and cytokine receptors, cytokine-cytokine receptor interactions, and COVID-19 with primary ontology functions for COVID-19, Kawasaki Disease/Takayasu arteritis, and aneurysm formation. These findings suggest a shared and overlapping expression of host immune transcriptomic pathways in large vessel inflammatory vasculitis, inflammatory thoracic aneurysms, MIS-C, Kawasaki disease and autoimmune aortitis, The study is unique in the use of multiplexed DSP to analyze DEGs and molecular pathway activation patterns in specific areas of the aortic wall.  This approach is considerably different than the more widely used bulk transcriptomic and single cell RNA methods, which measure DEGs in the entire aortic specimen.   Based on this pilot study it is possible that DSP might be useful in analyzing molecular mechanisms in specific zones of aortic wall in various types of aneurysms, which could lead to a better understanding of the molecular pathophysiology involved. 

Limitations.

This was a pilot study with only one patient and one control, and has many limitations. Based on the small sample size, the statistical significance of the findings should be considered marginal and the findings need to be validated across a larger number of patients. A major limitation is that the study does not have a normal aorta as a control. These findings only represent DEG between specific areas within the aortic wall across the AoC19 specimen, and DEGs in comparison to Giant Cell aortitis, and do not identify DEGs compared to a normal aorta. While DSP may have certain advantages in allowing the spatial comparison of gene expression in the microenvironment of tissue specimens, that is also a limitation, and the use of bulk transcriptomics and single cell RNA may be more robust in identifying major tissue wide transcriptomic patterns across a large number of specimens or patients. 

Conflict of Interest and Disclosures Statement:

Pass, Mezzano, Zhou, Tsirigos, Ramaswami, James,  Peterson, and Smith, have nothing to disclose. Dr. Galloway has intellectual property with Medtronic and receives royalties for valve repair devices. 

Funding Statement:

This study was supported by NYU Grossman School of Medicine, Department of Cardiothoracic Surgery Research Fund. The Experimental Pathology and Genome Technology Center core laboratories are a part of the NYU Grossman School of Medicine, Division of Advanced Research Technologies, and are funded in part by the National Institutes of Health/National Cancer Institute [5 P30CA16087].

Presentation:

Abstract presented at 2022 AATS Aortic Symposium: “Post-COVID-19 Aortitis with Aneurysm Formation.”

Institutional Review Board Approval:

The study was conducted under the NYU Grossman School of Medicine Lung Cancer Biomarker Center (#I8896) and underwent expedited IRB review. 

Data Availability Statement:

The GeoMx gene tag sequencing data sets generated in this study will be available at GEO, and Code will be available at GitHub. Microscopy image data is stored in public OMERO Plus and will be accessible through the NYU Data Catalog upon publication. 

References

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George Varvatsoulias

Dear editorial department: On behalf of our team, I hereby certify the reliability and superiority of the International Journal of Clinical Case Reports and Reviews in the peer review process, editorial support, and journal quality. Firstly, the peer review process of the International Journal of Clinical Case Reports and Reviews is rigorous, fair, transparent, fast, and of high quality. The editorial department invites experts from relevant fields as anonymous reviewers to review all submitted manuscripts. These experts have rich academic backgrounds and experience, and can accurately evaluate the academic quality, originality, and suitability of manuscripts. The editorial department is committed to ensuring the rigor of the peer review process, while also making every effort to ensure a fast review cycle to meet the needs of authors and the academic community. Secondly, the editorial team of the International Journal of Clinical Case Reports and Reviews is composed of a group of senior scholars and professionals with rich experience and professional knowledge in related fields. The editorial department is committed to assisting authors in improving their manuscripts, ensuring their academic accuracy, clarity, and completeness. Editors actively collaborate with authors, providing useful suggestions and feedback to promote the improvement and development of the manuscript. We believe that the support of the editorial department is one of the key factors in ensuring the quality of the journal. Finally, the International Journal of Clinical Case Reports and Reviews is renowned for its high- quality articles and strict academic standards. The editorial department is committed to publishing innovative and academically valuable research results to promote the development and progress of related fields. The International Journal of Clinical Case Reports and Reviews is reasonably priced and ensures excellent service and quality ratio, allowing authors to obtain high-level academic publishing opportunities in an affordable manner. I hereby solemnly declare that the International Journal of Clinical Case Reports and Reviews has a high level of credibility and superiority in terms of peer review process, editorial support, reasonable fees, and journal quality. Sincerely, Rui Tao.

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Rui Tao

Clinical Cardiology and Cardiovascular Interventions I testity the covering of the peer review process, support from the editorial office, and quality of the journal.

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Khurram Arshad

Clinical Cardiology and Cardiovascular Interventions, we deeply appreciate the interest shown in our work and its publication. It has been a true pleasure to collaborate with you. The peer review process, as well as the support provided by the editorial office, have been exceptional, and the quality of the journal is very high, which was a determining factor in our decision to publish with you.

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Gomez Barriga Maria Dolores

The peer reviewers process is quick and effective, the supports from editorial office is excellent, the quality of journal is high. I would like to collabroate with Internatioanl journal of Clinical Case Reports and Reviews journal clinically in the future time.

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Lin Shaw Chin

Clinical Cardiology and Cardiovascular Interventions, I would like to express my sincerest gratitude for the trust placed in our team for the publication in your journal. It has been a true pleasure to collaborate with you on this project. I am pleased to inform you that both the peer review process and the attention from the editorial coordination have been excellent. Your team has worked with dedication and professionalism to ensure that your publication meets the highest standards of quality. We are confident that this collaboration will result in mutual success, and we are eager to see the fruits of this shared effort.

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Maria Dolores Gomez Barriga

Dear Dr. Jessica Magne, Editorial Coordinator 0f Clinical Cardiology and Cardiovascular Interventions, I hope this message finds you well. I want to express my utmost gratitude for your excellent work and for the dedication and speed in the publication process of my article titled "Navigating Innovation: Qualitative Insights on Using Technology for Health Education in Acute Coronary Syndrome Patients." I am very satisfied with the peer review process, the support from the editorial office, and the quality of the journal. I hope we can maintain our scientific relationship in the long term.

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Dr Maria Dolores Gomez Barriga

Dear Monica Gissare, - Editorial Coordinator of Nutrition and Food Processing. ¨My testimony with you is truly professional, with a positive response regarding the follow-up of the article and its review, you took into account my qualities and the importance of the topic¨.

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Dr Maria Regina Penchyna Nieto

Dear Dr. Jessica Magne, Editorial Coordinator 0f Clinical Cardiology and Cardiovascular Interventions, The review process for the article “The Handling of Anti-aggregants and Anticoagulants in the Oncologic Heart Patient Submitted to Surgery” was extremely rigorous and detailed. From the initial submission to the final acceptance, the editorial team at the “Journal of Clinical Cardiology and Cardiovascular Interventions” demonstrated a high level of professionalism and dedication. The reviewers provided constructive and detailed feedback, which was essential for improving the quality of our work. Communication was always clear and efficient, ensuring that all our questions were promptly addressed. The quality of the “Journal of Clinical Cardiology and Cardiovascular Interventions” is undeniable. It is a peer-reviewed, open-access publication dedicated exclusively to disseminating high-quality research in the field of clinical cardiology and cardiovascular interventions. The journal's impact factor is currently under evaluation, and it is indexed in reputable databases, which further reinforces its credibility and relevance in the scientific field. I highly recommend this journal to researchers looking for a reputable platform to publish their studies.

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Dr Marcelo Flavio Gomes Jardim Filho

Dear Editorial Coordinator of the Journal of Nutrition and Food Processing! "I would like to thank the Journal of Nutrition and Food Processing for including and publishing my article. The peer review process was very quick, movement and precise. The Editorial Board has done an extremely conscientious job with much help, valuable comments and advices. I find the journal very valuable from a professional point of view, thank you very much for allowing me to be part of it and I would like to participate in the future!”

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Zsuzsanna Bene

Dealing with The Journal of Neurology and Neurological Surgery was very smooth and comprehensive. The office staff took time to address my needs and the response from editors and the office was prompt and fair. I certainly hope to publish with this journal again.Their professionalism is apparent and more than satisfactory. Susan Weiner

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Dr Susan Weiner