[email protected]
+1-(302)-520-2644
+1-(302)-520-2644

Pharmacology and Analytical Chemistry Profile of Dapagliflozin, Empagliflozin and Saxagliptin

  1. Home
  2. Journals
  3. Pharmaceutics and Pharmacology Research
  4. Archives
Submit Guidelines

Research Article | DOI: https://doi.org/10.31579/2693-7247/069

Pharmacology and Analytical Chemistry Profile of Dapagliflozin, Empagliflozin and Saxagliptin

  • Hany A. Batakoushy 1*
  • Mahmoud A. Omar 2
  • Hytham M. Ahmed 3
  • Mohamed A. Abdel Hamid 4
  • Mahmoud M. Sebaiy 5

1 Pharmaceutical Analysis Department, Faculty of Pharmacy, Menoufia University, Egypt.

2Department of Pharmacognosy and Pharmaceutical Chemistry, College of Pharmacy, Taibah University, Madinah, Saudi Arabia

3Department of Analytical Chemistry, Faculty of Pharmacy, Minia University, Egypt.

4 Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Tanta University, Egypt.

5Department of Medicinal Chemistry, Faculty of Pharmacy, Zagazig University, Zagazig, 44519, Egypt.

 

*Corresponding Author: Mahmoud M. Sebaiy, of Medicinal Chemistry, Faculty of Pharmacy, Zagazig University, Zagazig, 44519, Egypt.

Citation: Hany A. Batakoushy, Mahmoud A. Omar, Hytham M. Ahmed, Mohamed A. Abdel Hamid and Mahmoud M. Sebaiy (2022) Pharmacology and Analytical Chemistry Profile of Dapagliflozin, Empagliflozin and Saxagliptin,. J. Pharmaceutics and Pharmacology Research. 5(4); DOI: 10.31579/2693-7247/069

Copyright: © 2022, Mahmoud M. Sebaiy, this is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Received: 18 February 2022 | Accepted: 08 March 2022 | Published: 06 April 2022

Keywords: diabetes; dapagliflozin; empagliflozin; saxagliptin; pharmacology; analytical chemistry.

Abstract

Diabetes mellitus is a worldwide disease that requires special and continuous medical care. Many classes of oral hypoglycemic drugs are currently used; however, the treatment strategy depends on the nature of diabetes type, pharmacological properties of the used drugs in addition to the clinical characteristics of the patient. As such, in this literature review, we will shed the light on the pharmacology and analytical chemistry profile of certain oral hypoglycemic drugs specifically Dapagliflozin, Empagliflozin and Saxagliptin that got attention in the last decade. Mode of action and most of up-to-date reported methods that have been developed for determination of these important anti-diabetic drugs in their pure form, combined form with other drugs, combined form with degradation products, and in biological samples are mentioned in detail.

Introduction

Diabetes mellitus (DM) is a lifelong disease that requires continuous medical care. Chronic long-term hyperglycemia associated with diabetes that causes serious complications lead to either drug monitoring in the line of treatment single or combined dosage form. Type 2 diabetes mellitus (T2DM) is a worldwide problem that affects about 8% of the adults all over the world, with predictions of more than 400 million cases by 2030 [1]. The prevalence of this type of DM implies an urgent need for finding treatments and preventative strategies. The disease results from progressive β-cell dysfunction in the presence of insulin resistance, and this leads to a progressive decrease in plasma glucose homeostasis, increased glucagon secretion, gluconeogenesis, and renal glucose reabsorption and reduced incretin response. Treatments recommended by the American diabetes association and the European association for the study of diabetes include drugs affecting all of the above processes [2].

Monotherapy using oral medications should be concomitant with management of intensive lifestyle. When glycemic control is no longer maintained with a single drug, the addition of a second or third oral hypoglycemic drugs usually more effective than switching to another single drug.

Hypoglycemic drugs comprise a pharmacologically and chemically heterogeneous drugs groups. There are different classes of oral hypoglycemic drugs and their selection depends on the nature of diabetes, pharmacological properties of the compounds such as efficacy, safety profile and the clinical characteristics of the patient (stage of disease, age and bodyweight) [3]. These drugs, which exhibit different modes of action may be used as a monotherapy or in various combinations.

Gliflozins

Gliflozins are the newest class of approved oral hypoglycemic agents that specifically inhibit sodium glucose co-transporter 2 function in the kidney, thus preventing renal glucose reabsorption and increasing glycosuria in diabetic patients while reducing hyperglycemia with hypoglycemia minimal risk. They reduce glycated hemoglobin and exert favorable effects beyond glucose control with consistent body weight, serum uric acid reductions and blood pressure. The main drugs from this group are dapagliflozin (DGF) and empagliflozin (EGF) [4-8].

Gliptins

Gliptins represent a new class of drugs that improve the health of beta cells and suppress glucagon, resulting in improved post-prandial and fasting hyperglycemia. They function by augmenting the incretin system inhibiting their metabolism by dipeptidyl peptidase 4. They are also safe and do not cause significant hypoglycemia, making it a unique class of drugs. The main drug from this group is Saxagliptin (SXG) [9].

Mechanism of sodium glucose co-transporter 2 Inhibitors

SGLT2 is a human protein that facilitates glucose reabsorption from the kidney. SGLT2 is a low-affinity, high-capacity glucose transporter which is located in the kidney proximal tubules. It is responsible for 90 % of glucose reabsorption. Inhibition of SGLT2 can lead to a decrease in blood glucose due to the increase in excretion of renal glucose. The mechanism of action of this new class of drugs also offers more glucose control by allowing an increase in insulin sensitivity and uptake of glucose in the muscles, decreased gluconeogenesis and improved insulin release from the beta cells. Drugs in the SGLT2 inhibitors class include DGF and EGF, these drugs in this class are FDA approved for the treatment of T2DM.

The usage of studied drugs as tinny amount and very diluted in biological matrix to analyze studied drugs in low levels to be applied in their assay in biological samples and give challenge to find suitable method for analysis of these drugs. Moreover, it is well-known that spectrofluorimetric methods are much more sensitive than spectrophotometric methods [10]. Furthermore, studied drugs analysis in the required low level in plasma samples by measuring the native fluorescence of each DGF and EGF and needed the use of a fluorogenic derivatizing reagent to enhance the sensitivity of the analysis by producing a highly sensitive fluorophore. Therefore, benzofurazan derivative was used in this study for the first time to develop a new validated and sensitive spectrofluorimetric analytical method for studied drug analysis in all sample matrices either pure or biological.

A way to speed up the validation process consists of the use of experimental design, which can be very useful and advantageous for both the evaluation and the optimization of some performance parameters. Experimental design techniques are powerful tools for the exploration of multivariate systems [11-13]. Statistical design is a way of choosing experiments; efficiently and systematically to give reliable and coherent information.

From a statistical standpoint, design means construction of experiments so that the analysis of results yields the maximum amount of information that can be extracted from the experiments. More specifically, experimental design helps the researcher to verify if changes in factor values produce a statistically significant variation of the observed response, and this approach can be used each time it is necessary to have this type of information. Typically, experimental design techniques are used to understand the effect of several variables on a system by a well-defined mathematical model. The strategy is most effective if statistical design is used in most or all stages of development and not only for screening or optimizing the process.

A systematic use of statistical design in developing a method ensures traceability, supports validation, and makes the subsequent confirmatory validation much easier and more certain. In fact, it is difficult to completely separate method optimization from validation since these two areas are linked, and sometimes a compromise has to be found [14].

There is no reported voltammetry study for DGF analysis in the literature. DGF acts as electroactive compound, and it is easily oxidized. The development of electrochemical-based sensors is considered important. Electrochemical sensors have the reputation of being small, quick, cheap, and easy to use for analytical applications, but their designing to be sensitive and selective for analyte of interest is a challenge. The rapid nature of electrochemistry makes it appealing for use in medical applications where quick tests are necessary for medical diagnostics, to ensure drug quality, and to understand dynamics of molecular changes during diseases. Therefore, polymer films modified electrodes received a great attention recently due to their wide applications in the fields of chemical sensors and biosensors [15-19]. Such modified electrodes can significantly improve the electrocatalytic properties of substrates, decrease the over potential, increase the reaction rate and improve the stability and reproducibility of the electrode response in the area of electro analysis [20-29].

The incorporation of metallic nanoparticles (NPs) into conductive polymers is of great interest because of their strong electronic interactions between NPs and the polymer matrices. It has been reported that the electrocatalytic properties and conductivities of NPs could be enhanced by the conductive polymeric matrices [19]. Previously, poly 1,5‑diaminonaphthalene (PDAN) was prepared in aqueous and nonaqueous media at glassy carbon (GC) electrode [20, 22]. The electrodeposition of metal NPs in the polymer films improves their tolerance towards electrooxidation of small molecules [30]. Herein, in this perspective, PDAN films were prepared at the surface of GC electrode, followed by monometallic platinum (Pt) or palladium (Pd) NPs electrodeposition. Suitability of these new composite NPs modified polymeric GC electrodes towards the electrocatalytic oxidation of studied drugs have been studied by electrochemical measurements.

On the other hand, The combination therapy of DGF and SXG was shown to be superior in lowering blood glucose when compared with either of the monotherapy regimens [31]. However, this combination therapy leads to a big challenge in pharmaceutical and biomedical analysis area. Therefore, it is important to get a valid analytical separation technique suitable for the analysis of these drugs in presence of each other. Also, the analysis should be valid in presence of their degradation products and also in pharmaceutical dosage form. High performance thin-layer chromatography (HPTLC) has several advantages over HPLC in some analysis. As HPTLC, separations are generally more efficient than HPLC. Also, it takes short time for analysis. Moreover, it requires few nanoliter injection volumes. Furthermore, minimal use of solvent and no prior extraction steps compared to HPLC [32, 33].

Chemistry of the investigated oral hypoglycemic drugs

The chemical structures and pharmacokinetic parameters of the investigated drugs and their chemical names are presented in Tables 1 and 2.

Table 1. The names, chemical structures and nomenclature of the studied oral hypoglycemic drugs.
Table 2. Pharmacokinetic and physicochemical parameters of the studied oral antidiabetic drugs.

Analytical methods for the determination of certain antidiabetic drugs: 

Pharmaceutical analysis has become one of the most important stages in the therapeutic process. Drug analysis includes analytical investigations of bulk drug materials, intermediate products, drug formulations, impurities and degradation products. Analytical techniques play a significant role in understanding the chemical stability of the drug, in evaluating the toxicity of some impurities and in assessing thecontent of drug in formulations.

Also, they are fundamental tools in pharmacokinetic studies where the analysis of a drug and its metabolites in biological fluids must be performed. This review presentsanalytical procedures such as spectrophotometric (UV/VIS) methods, HPLC and HPTLC methods. It is based on a review of the literature from (2009-2020).

The studied drugs (DGF, EGF and SXG) have not an official method in any pharmacopeia. The reported method included;

Spectroscopic methods

Ultraviolet and visible spectrophotometric methods:

In literature survey, either spectrophotometric methods have been reported for determination of the studied drugs in pure forms or in their pharmaceutical preparations. These reported methods are summarized in Table 3.

Table 3. Spectrophotometric (UV/VIS) methods for the analysis of DGF, EGF and SXG in bulk materials and formulations.

Spectrofluorimetric methods

The reported spectrofluorimetric methods for the investigated drugs as the following;

Spectrofluorimetric methods of SAX and vildagliptin in bulk and pharmaceutical preparations using NBD-Cl fluorogenic reagent at λex of 468 and 465 nm for SAX and VDG, respectively. Fluorescence intensity at λem of 542 and 540 nm for SAX and VDG, respectively [49]. A simple and highly sensitive and robust spectrofluorimetric method was developed for the determination of sitagliptin phosphate and SAX. The proposed method is based on Hantzsch reaction of both drugs. Fluorescent products in presence of sodium dodecyl sulfate micellar system as additive to enhance the obtained fluorescence at 483 nm after excitation at 419 nm for both drugs[50]. 

High Performance Thin-Layer Chromatography (HPTLC):

A high-performance thin-layer chromatographic method was developed for simultaneous determination of EGF and Linagliptin. The proposed method was applied successfully to the pharmaceutical analysis using precoated silica plates coated with 0.2 mm layers of silica gel 60 F254 and methanol: toluene: ethyl acetate (2:4:4, v/v/v) was selected as mobile phase [51]. Stability indicating HPTLC-MS method for estimation of EGF in pharmaceutical dosage form using silica plates coated with 0.2 mm layers of silica gel 60 F254 and toluene : methanol (7:3, v/ v) was selected as mobile phase [52].

HPTLC was developed for the quantitative analysis of SXG in active pharmaceutical ingredients (APIs) and pharmaceutical dosage forms. The method was achieved using silica gel aluminum plate 60 F254 (10 × 10 cm) as stationary phase and methanol: chloroform (6:4 v/v) as mobile phase [53]. HPTLC method for the simultaneous determination of metformin, SXG and DGF in pharmaceuticals. Separation was performed using aluminum HPTLC sheets coated with silica gel 60 F254 with a mobile phase consisting of a mixture of acetonitrile: 1% w/v ammonium acetate in methanol (9: 1, v/v), scanning was performed at 210 nm[54].

HPTLC analytical method for simultaneous estimation of DGF and SXG in synthetic mixture using silica gel aluminum plate 60F254(10×10cm)as stationary phase and chloroform: ethyl acetate: methanol: ammonia (6:2:2:2 drops) as mobile phase[55]. HPTLC method was developed for the determination of either linagliptin, SXG or vildagliptin in their binary mixtures with metformin in pharmaceutical preparations. Separation was carried out on Merck HPTLC aluminum sheets of silica gel 60F254 using methanol: 0.5% w/v aqueous ammonium sulfate (8: 2, v/v) as mobile phase [56].

High Performance Liquid Chromatography (HPLC):

Various HPLC methods had been reported for the determination of the studied drugs either alone or in combination with others active ingredients in dosage forms or in biological fluids. Table 4: summarized the most recent applications of this technique.

Table 4. HPLC methods for the analysis of DGF, EGF and SXG in bulk materials and formulations.

 

Capillary electrophoresis methods

A capillary electrophoretic method coupled to a diode array detector (CE-DAD) was developed and validated for the simultaneous determination of metformin hydrochloride, SAX and DGF. The proposed method was used for the determination of these drugs in combinations namely, SXG/metformin, DGF/metformin and SXG/DGF. CE separation was performed on a fused silica capillary with background electrolyte consisting of 30mM phosphate buffer (pH 6.0). The compounds were detected at 203nm for SXG/DGF and 250 nm for metformin. The method was linear in the concentration ranges of 10-200, 1.25-50 and 7.5-100 μg/mL for SAX, DGF and metformin, respectively [86].

Electrochemical method:

The literature is devoid of any electrochemical methods for the quantitation of the studied drugs. The first, sensitive and accurate potentiometric sensor for the selective determination of SXG in the presence of either its active metabolite 5‑hydroxy SXG, other co-administered or structurally related drugs [87].

Conclusion:

This literature review represents the mode of action in addition to an up to date survey about all reported methods that have been developed for determination of Dapagliflozin, Empagliflozin and Saxagliptin in their pure form, combined form with other drugs, combined form with degradation products, and in biological samples such as liquid chromatography, spectrophotometry, spectroflourimetry, electrochemistry, etc.

References

  1. Zheng Y, S.H. Ley, and F.B. Hu , (2018).Global aetiology and epidemiology of type 2 diabetes mellitus and its complications. Nature Reviews Endocrinology, 14(2): 88.
    View at Publisher | View at Google Scholar
  2. Davies M.J., D.A. D’Alessio, J. Fradkin, W.N. Kernan, C. Mathieu, G. Mingrone, P. Rossing, A. Tsapas, D.J. Wexler, and J.B. Buse (2018). Management of hyperglycaemia in type 2 diabetes, 2018. A consensus report by the American Diabetes Association (ADA) and the European Association for the Study of Diabetes (EASD). Diabetologia, , 61(12): 2461-2498.
    View at Publisher | View at Google Scholar
  3. Inzucchi S.E., R.M. Bergenstal, J.B. Buse, M. Diamant, E. Ferrannini, M. Nauck, A.L. Peters, A. Tsapas, R. Wender, and D.R. Matthews(2015). Management of hyperglycaemia in type 2 diabetes, 2015: a patient-centred approach. Update to a position statement of the American Diabetes Association and the European Association for the Study of Diabetes. Diabetologia, , 58(3): 429-442.
    View at Publisher | View at Google Scholar
  4. Bonner C., J. Kerr-Conte, V. Gmyr, G. Queniat, E. Moerman, J. Thévenet, C. Beaucamps, N. Delalleau, I. Popescu, and W.J. Malaisse( 2015). Inhibition of the glucose transporter SGLT2 with dapagliflozin in pancreatic alpha cells triggers glucagon secretion. Nature medicine, 21(5): 512.
    View at Publisher | View at Google Scholar
  5. Freeman J.S (2013).Review of insulin-dependent and insulin-independent agents for treating patients with type 2 diabetes mellitus and potential role for sodium-glucose co-transporter 2 inhibitors. Postgraduate medicine, , 125(3): 214-226.
    View at Publisher | View at Google Scholar
  6. Grempler R., L. Thomas, M. Eckhardt, F. Himmelsbach, A. Sauer, D. Sharp, R. Bakker, M. Mark, T. Klein, and P. Eickelmann, Empagliflozin, a novel selective sodium glucose cotransporter‐2 (SGLT‐2) inhibitor: characterisation and comparison with other SGLT‐2 inhibitors. Diabetes, Obesity and Metabolism, , 14(1): 83-90.
    View at Publisher | View at Google Scholar
  7. Neumiller J.J(2014). Empagliflozin: a new sodium-glucose co-transporter 2 (SGLT2) inhibitor for the treatment of type 2 diabetes. Drugs in context, 3.
    View at Publisher | View at Google Scholar
  8. Washburn W.N. and S.M. Poucher(2013), Differentiating sodium-glucose co-transporter-2 inhibitors in development for the treatment of type 2 diabetes mellitus. Expert opinion on investigational drugs, , 22(4): 463-486.
    View at Publisher | View at Google Scholar
  9. Scirica B.M, E. Braunwald, I. Raz, M.A. Cavender, D.A. Morrow, P. Jarolim, J.A. Udell, O. Mosenzon, K. Im, and A.A. Umez-Eronini, Heart failure, saxagliptin, and diabetes mellitus: observations from the SAVOR-TIMI 53 randomized trial. Circulation , 130(18): 1579-1588.
    View at Publisher | View at Google Scholar
  10. Derayea S.M., A.A. Hamad, D.M. Nagy, D.A. Nour-Eldeen, H.R.H. Ali, and R. Ali(2018). Improved spectrofluorimetric determination of mebendazole, a benzimidazole anthelmintic drug, through complex formation with lanthanum (III); Application to pharmaceutical preparations and human plasma. Journal of Molecular Liquids, 272: 337-343.
    View at Publisher | View at Google Scholar
  11. Carlson R. and J.E. Carlson(2005). Design and optimization in organic synthesis. Vol. 24.Elsevier.
    View at Publisher | View at Google Scholar
  12. Lewis G., D. Mathieu, and R. Phan-Tan-Luu(1999). Mixtures in a constrained region of interest. Pharmaceutical Experimental Design. New York: Marcel Dekker, 413-54.
    View at Publisher | View at Google Scholar
  13. Montgomery D.C(1997). Design and analysis of experiments John Wiley & Sons. Inc., New York, 2001: 200.1.
    View at Publisher | View at Google Scholar
  14. Furlanetto S., S. Orlandini, P. Mura, M. Sergent, and S. Pinzauti(2003). How experimental design can improve the validation process. Studies in pharmaceutical analysis. Analytical and bioanalytical chemistry, 377(5): 937-944.
    View at Publisher | View at Google Scholar
  15. Ensafi A.A., B. Rezaei, M. Amini, and E. Heydari-Bafrooei(2012). A novel sensitive DNA–biosensor for detection of a carcinogen, Sudan II, using electrochemically treated pencil graphite electrode by voltammetric methods. Talanta, 88: 244-251.
    View at Publisher | View at Google Scholar
  16. Gong W, Z.-Y. Dou, P. Liu, X.-Y. Cai, and X.-q. He(2012). Simultaneous determination of dopamine, ascorbic acid by polyethylene oxide (PEO) covalently modified glassy carbon electrode. Journal of Electroanalytical Chemistry, 666: 62-66.
    View at Publisher | View at Google Scholar
  17. Hadi M. and A. Rouhollahi(2012). Simultaneous electrochemical sensing of ascorbic acid, dopamine and uric acid at anodized nanocrystalline graphite-like pyrolytic carbon film electrode. Analytica chimica acta, , 721: 55-60.
    View at Publisher | View at Google Scholar
  18. Yang L., S. Liu, Q. Zhang, and F. Li(2012). Simultaneous electrochemical determination of dopamine and ascorbic acid using AuNPs@ polyaniline core–shell nanocomposites modified electrode. Talanta, , 89: 136-141.
    View at Publisher | View at Google Scholar
  19. Yuan Y., H. Li, S. Han, L. Hu, S. Parveen, H. Cai, and G. Xu(2012). Immobilization of tris (1, 10-phenanthroline) ruthenium with graphene oxide for electrochemiluminescent analysis. Analytica chimica acta, 720: 38-42.
    View at Publisher | View at Google Scholar
  20. Abdel-Azzem M., U. Yousef, and G. Pierre(1998). A cyclic voltammetric and coulometric study of a modified electrode prepared by electrooxidative polymerization of1, 5-diaminonaphthalene in aqueous acidic medium. European polymer journal, 34(5-6): 819-826.
    View at Publisher | View at Google Scholar
  21. Adzic R., M. Hsiao, and E. Yeager(1989). Electrochemical oxidation of glucose on single crystal gold surfaces. Journal of electroanalytical chemistry and interfacial electrochemistry, 260(2): 475-485.
    View at Publisher | View at Google Scholar
  22. Azzem M.A., U. Yousef, D. Limosin, and G. Pierre(1994), Electropolymerization of 1, 5-diaminonaphthalene in acetonitrile and in aqueous solution. Synthetic metals, 63(1): 79-81.
    View at Publisher | View at Google Scholar
  23. Bai Y., W. Yang, Y. Sun, and C. Sun, Sensors Actuators. B, 2008, 134: 471.
    View at Publisher | View at Google Scholar
  24. Barrera C., I. Zhukov, E. Villagra, and F. Bedioui, MA P. aez, J(2006). Costamaga, JH Zagal. Journal of Electroanalytical Chemistry, 589: 212.
    View at Publisher | View at Google Scholar
  25. Jafarian M., F. Forouzandeh, I. Danaee, F. Gobal, and M(2009). Mahjani, Electrocatalytic oxidation of glucose on Ni and NiCu alloy modified glassy carbon electrode. Journal of Solid State Electrochemistry, 2009, 13(8): 1171-1179.
    View at Publisher | View at Google Scholar
  26. Kalimuthu P. and S.A. John(2010), Simultaneous determination of ascorbic acid, dopamine, uric acid and xanthine using a nanostructured polymer film modified electrode. Talanta, 80(5): 1686-1691.
    View at Publisher | View at Google Scholar
  27. Tominaga M., T. Shimazoe, M. Nagashima, H. Kusuda, A. Kubo, Y. Kuwahara, and I. Taniguchi(2006). Electrocatalytic oxidation of glucose at gold–silver alloy, silver and gold nanoparticles in an alkaline solution. Journal of Electroanalytical Chemistry, 590(1): 37-46.
    View at Publisher | View at Google Scholar
  28. Wang C., C. Yang, Y. Song, W. Gao, and X. Xia(2005), Adsorption and direct electron transfer from hemoglobin into a three‐dimensionally ordered macroporous gold film. Advanced Functional Materials, 15(8): 1267-1275.
    View at Publisher | View at Google Scholar
  29. Wang H.-S., T.-H. Li, W.-L. Jia, and H.-Y. Xu(2006). Highly selective and sensitive determination of dopamine using a Nafion/carbon nanotubes coated poly (3-methylthiophene) modified electrode. Biosensors and Bioelectronics, 22(5): 664-669.
    View at Publisher | View at Google Scholar
  30. Pournaghi-Azar M. and B. Habibi(2007). Electrocatalytic oxidation of methanol on poly (phenylenediamines) film palladized aluminum electrodes, modified by Pt micro-particles: comparison of permselectivity of the films for methanol. Journal of Electroanalytical Chemistry, 601(1-2): 53-62.
    View at Publisher | View at Google Scholar
  31. Singh-Franco D(2015). Potential for dipeptidyl peptidase-4 inhibitor and sodium glucose cotransporter 2 inhibitor single-pill combinations. Expert review of endocrinology & metabolism, 10(3): 305-317.
    View at Publisher | View at Google Scholar
  32. Mohamed A.-M.I., M.A. Omar, S.M. Derayea, M.A. Hammad, and A.A. Mohamed(2018) Innovative thin-layer chromatographic method combined with fluorescence detection for specific determination of Febuxostat: Application in biological fluids. Talanta, 176: 318-328.
    View at Publisher | View at Google Scholar
  33. Saraya R.E., M. Elhenawee, and H. Saleh(2018).Development of a highly sensitive high‐performance thin‐layer chromatography method for the screening and simultaneous determination of sofosbuvir, daclatasvir, and ledipasvir in their pure forms and their different pharmaceutical formulations. Journal of separation science, 41(18): 3553-3560.
    View at Publisher | View at Google Scholar
  34. Mante G.V., K.R. Gupta, and A.T. Hemke(2017). Estimation of Dapagliflozin from its tablet formulation by UV-Spectrophotometry. Pharm Methods, 8(2): 102-107.
    View at Publisher | View at Google Scholar
  35. Karuna P., E. China, and M. Basaveswara Rao(2015). Unique UV spectrophotometric method for reckoning of Dapagliflozin in bulk and pharmaceutical dosage forms. J Chem Pharm Res, 7(9): 45-9.
    View at Publisher | View at Google Scholar
  36. Jani B., K. Shah, and P. Kapupara(2015). Development and Validation of UV Spectroscopic First Derivative Method for Simultaneous Estimation of Dapagliflozin and Metformin Hydrochloride in Synthetic Mixture. J Bioequiv, 1(1): 102.
    View at Publisher | View at Google Scholar
  37. Manasa S., K. Dhanalakshmi, R. Nagarjuna, and S. Sreenivasa(2014) Method development and validation of dapagliflozin in API by RP-HPLC and UV-spectroscopy. International Journal of Pharmaceutical Science and Drug Research, 6: 250-252.
    View at Publisher | View at Google Scholar
  38. Suthar A.M, L.M. Prajapati, A.K. Joshi, J.R. Patel, M.L. Kharodiya, and L. Prajapati(2018). Estimation of Saxagliptin hydrochloride and Dapagliflozin propendiol monohydrate in combined dosage form. JIAPS, 3(2): 01-07.
    View at Publisher | View at Google Scholar
  39. Lotfy H.M, D. Mohamed, and M.S. Elshahe(2019). Novel univariate spectrophotometric determination of the recently released solid dosage form comprising dapagliflozin and saxagliptin via factorized response spectra: Assessment of the average content and dosage unit uniformity of tablets. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy.
    View at Publisher | View at Google Scholar
  40. Padmaja N., M.S. Babu, and G(2016). Veerabhadram, Development and validation of UV spectrophotometric method for Simultaneous estimation of Empagliflozin and Metformin hydrochloride in bulk drugs and combined dosage forms. Der Pharmacia Lettre , 8(13): 207-213.
    View at Publisher | View at Google Scholar
  41. Jyothirmai N., B. Nagaraju, and M. Anil Kumar(2016). Novel uv and visible spectrophotometric methods for the analysis of empagliflozin a type 2 diabetic drug in bulk and pharmaceutical formulations. Journal de afrikana, 3(1): 177-187.
    View at Publisher | View at Google Scholar
  42. Patil S.D., S.K. Chaure, and S. Kshirsagar(2017). Development and validation of UV spectrophotometric method for Simultaneous estimation of Empagliflozin and Metformin hydrochloride in bulk drugs. Asian Journal of Pharmaceutical Analysis, 7(2): 117-123.
    View at Publisher | View at Google Scholar
  43. Ayoub B.M(2016,). Development and validation of simple spectrophotometric and chemometric methods for simultaneous determination of empagliflozin and metformin: Applied to recently approved pharmaceutical formulation. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 168: 118-122.
    View at Publisher | View at Google Scholar
  44. Shaker L(2016). Development of Economic UV Spectrophotometric method for Determination of Linagliptin in its Tertiary Mixture with Empagliflozin and Metformin: Comparision to Economic pharmaceutical Analysis Literature. Scholars Research Library, 8(13): 267-269.
    View at Publisher | View at Google Scholar
  45. Kalaichelvi R. and E. Jayachandran(2011). Validated spectroscopic method for estimation of saxagliptin in pure and from tablet formulation. Int J Pharm Pharm Sci, 3(3): 179-180.
    View at Publisher | View at Google Scholar
  46. El-Bagary R.I., E.F. Elkady, and B.M. Ayoub(2012).Spectrophotometric methods based on charge transfer complexation reactions for the determination of saxagliptin in bulk and pharmaceutical preparation. International journal of biomedical science: IJBS, , 8(3): 204.
    View at Publisher | View at Google Scholar
  47. Narendra N. and J. Govinda(2012). Development and validation of uv-vis spectroscopy method for simultaneous estimation of saxagliptin hydrochloride and metformin hydrochloride in active pharmaceutical ingredient. J Pharm Educ Res, 3(2): 19-23.
    View at Publisher | View at Google Scholar
  48. Kalaichelvi R. and E. Jayachandran(2011). Validated Spectroscopic method for the estimation of Saxagliptinin pure and from tablet formulation. Int J Pharm Pharm Sci,3(3): 179-180.
    View at Publisher | View at Google Scholar
  49. Moneeb M.S(2013). Spectrophotometric and spectrofluorimetric methods for the determination of saxagliptin and vildagliptin in bulk and pharmaceutical preparations. Bulletin of Faculty of Pharmacy, Cairo University, 51(2): 139-150.
    View at Publisher | View at Google Scholar
  50. Barseem A., H. Ahmed, Y. El-Shabrawy, and F. Belal(2019). The use of SDS micelles as additive to increase fluorescence analysis of sitagliptin and saxagliptin derivatives in their tablets and human plasma. Microchemical Journal, 146: 20-24.
    View at Publisher | View at Google Scholar
  51. Bhole R., S. Wankhede, and M. pandey(2017). Stability indicating HPTLC method for simultaneous estimation of empagliflozin and linagliptin in pharmaceutical formulation. Analytical Chemistry Letters, 7(1): 76-85.
    View at Publisher | View at Google Scholar
  52. Bhole R.P. and F.R. Tamboli(2018). Development and Validation of Stability Indicating HPTLC-MS Method for Estimation of Empagliflozin in Pharmaceutical Dosage Form. Analytical Chemistry Letters, 8(2): 244-256.
    View at Publisher | View at Google Scholar
  53. Srividya S., E. Swetha, and C. Veeresham(2015) Development and validation of a high performance thin layer chromatographic method for quantitative analysis of saxagliptin. American journal of analytical chemistry, 6(10): 797.
    View at Publisher | View at Google Scholar
  54. Afnan E. Abdelrahman H.M.M., Nourah Z(2019). Alzoman, HPTLC Method for the Determination of Metformin Hydrochloride, Saxagliptin Hydrochloride, and Dapagliflozin in Pharmaceuticals. Current Analytical Chemistry, 15(1).
    View at Publisher | View at Google Scholar
  55. Shveta H. Parmar* D.S.V.L, Dr. Sachin B. Narkhede(2018). Development and validation of UV-spectroscopic first derivative and high performance thin layer chromatography analytical methods for simultaneous estimation of dapagliflozin propanediol monohydrate and saxagliptin hydrochloride in synthetic mixture,. ejbps,5(5): 668-684.
    View at Publisher | View at Google Scholar
  56. El-Kimary E.I., D.A. Hamdy, S.S. Mourad, and M.A. Barary(2015). HPTLC determination of three gliptins in binary mixtures with metformin. Journal of chromatographic science, 54(1): 79-87.
    View at Publisher | View at Google Scholar
  57. Debata J., S. Kumar, S.K. Jha, and A. Khan(2017). A New RP-HPLC method development and validation of dapagliflozin in bulk and tablet dosage form. Int J Drug Dev & Res, , 9(2): 48-51.
    View at Publisher | View at Google Scholar
  58. Aubry A.-F., H. Gu, R. Magnier, L. Morgan, X. Xu, M. Tirmenstein, B. Wang, Y. Deng, J. Cai, and P. Couerbe(2010). Validated LC–MS/MS methods for the determination of dapagliflozin, a sodium-glucose co-transporter 2 inhibitor in normal and ZDF rat plasma. Bioanalysis, 2(12): 2001-2009.
    View at Publisher | View at Google Scholar
  59. Sanagapati M., K. Dhanalakshmi, G. Nagarjunareddy, and S. Sreenivasa(2014). Development and validation of a RP-HPLC method for the estimation of dapagliflozin in API. International Journal of Pharmaceutical Sciences and Research, 5(12): 5394.
    View at Publisher | View at Google Scholar
  60. Manoharan G., A.M. Ismaiel, and Z.M. Ahmed (2018).Stabilityindicating RP-HPLC method development for simultaneous determination & estimation of dapagliflozin in raw & tablet formulation. Chemistry Research Journal, 3(2): 159-164.
    View at Publisher | View at Google Scholar
  61. Urooj A., P.S. Sundar, R. Vasanthi, M.A. Raja, K.R. Dutt, K. Rao, And H. Ramana(2017). Development And Validation Of Rp-Hplc Method For Simultaneous Estimation Of Dapagliflozin And Metformin In Bulk And In Synthetic Mixture. World journal of pharmacy and pharmaceutical sciences, 6(7): 2139-2150.
    View at Publisher | View at Google Scholar
  62. Singh N., P. Bansal, M. Maithani, and Y. Chauhan(2018), Development and validation of a stability-indicating RP-HPLC method for simultaneous determination of dapagliflozin and saxagliptin in fixed-dose combination. New Journal of Chemistry, 2018, 42(4): 2459-2466.
    View at Publisher | View at Google Scholar
  63. Kommineni V., K. Chowdary, and S. Prasad(2017), Development Of A New Stability Indicating Rp-Hplc Method For Simultaneous Estimation Of Saxagliptine And Dapagliflozin And Its Validation As Per Ich Guidelines. Indo American Journal Of Pharmaceutical Sciences, 4(9): 2920-2932.
    View at Publisher | View at Google Scholar
  64. Donepudi S. and S. Achanta(2019), Simultaneous Estimation of Saxagliptin and Dapagliflozin in Human Plasma by Validated High Performance Liquid Chromatography-Ultraviolet Method. Turkish Journal of Pharmaceutical Sciences,16(2): 227.
    View at Publisher | View at Google Scholar
  65. Khalil G.A., I. Salama, M.S. Gomaa, and M.A. Helal(2018), Validated Rp-Hplc Method For Simultaneous Determination Of Canagliflozin, Dapagliflozin, Empagliflozin And Metformin. International Journal of Pharmaceutical, Chemical & Biological Sciences, , 8(1).
    View at Publisher | View at Google Scholar
  66. Deepan T. and M.D. Dhanaraju(2018), Stability indicating HPLC method for the simultaneous determination of dapagliflozin and saxagliptin in bulk and tablet dosage form. Current Issues in Pharmacy and Medical Sciences, 31(1): 39-43.
    View at Publisher | View at Google Scholar
  67. Bhagavanji N(2012). Development and validation of stability indicating LC method for the simultaneous estimation of metformin and saxagliptin in combined dosage form. VSRD International Journal of Technical & Non-Technical Research, 3(11): 1-19.
    View at Publisher | View at Google Scholar
  68. Caglar S. and A. Alp(2014), A validated high performance liquid chromatography method for the determination of saxagliptin and metformin in bulk, a stability indicating study. J Anal Bioanal Tech S, 12: 2.
    View at Publisher | View at Google Scholar
  69. Demers R., H. Gu, L.J. Christopher, H. Su, L. Cojocaru, D.W. Boulton, M. Kirby, B. Stouffer, W.G. Humphreys, and M.E. Arnold(2012), Liquid chromatography and tandem mass spectrometry method for the quantitative determination of saxagliptin and its major pharmacologically active 5-monohydroxy metabolite in human plasma: method validation and overcoming specific and non-specific binding at low concentrations. Journal of Chromatography B, 889: 77-86.
    View at Publisher | View at Google Scholar
  70. Gao J.w., Y.m. Yuan, Y.s. Lu, and M.c. Yao(2012).Development of a rapid UPLC‐MS/MS method for quantification of saxagliptin in rat plasma and application to pharmacokinetic study. Biomedical Chromatography, 26(12): 1482-1487.
    View at Publisher | View at Google Scholar
  71. Mohammad M.A.-A., E.F. Elkady, and M.A. Fouad(2012), Development and validation of a reversed-phase column liquid chromatographic method for simultaneous determination of two novel gliptins in their binary mixtures with Metformin. European Journal of Chemistry, 3(2): 152-155.
    View at Publisher | View at Google Scholar
  72. Shah P.A., J.V. Shah, M. Sanyal, and P.S. Shrivastav(2017). LC–MS/MS analysis of metformin, saxagliptin and 5‐hydroxy saxagliptin in human plasma and its pharmacokinetic study with a fixed‐dose formulation in healthy Indian subjects. Biomedical Chromatography, 31(3): e3809.
    View at Publisher | View at Google Scholar
  73. Yunoos M. and D.G. Sankar(2015). A validated stability indicating high-performance liquid chromatographic method for simultaneous determination of metformin Hcl and dapagliflozin in bulk drug and tablet dosage form. Asian J Pharm Clin Res, 8(3): 320-326.
    View at Publisher | View at Google Scholar
  74. Daswadkar S.C., M.A. Roy, S.G. Walode, and C. Mahendra Kumar(2016). Quality by design approach for the development and validation of saxagliptin by RP-HPLC with application to formulated forms. Int J Pharm Sci, 7(4): 1670-1677.
    View at Publisher | View at Google Scholar
  75. Aswini R.E., MM Babu, P Srinivasa(2018), A Review on Analytical Methods for Estimation of Dapagliflozin and Saxagliptin in Bulk and in Pharmaceutcal Dosage Forms. IJRPC, 8(3): 460-468
    View at Publisher | View at Google Scholar
  76. Prasad P., K. Satyanaryana, and G. Krishnamohan(2015).Development and Validation of a Method for Simultaneous Determination of Metformin and Saxagliptin in a Formulation by RP-HPLC. American Journal of Analytical Chemistry, 6(11): 841.
    View at Publisher | View at Google Scholar
  77. Abdel-Ghany M.F., O. Abdel-Aziz, M.F. Ayad, and M.M. Tadros(2017). New LC–UV methods for pharmaceutical analysis of novel anti-diabetic combinations. Acta Chromatographica,29(4): 448-452.
    View at Publisher | View at Google Scholar
  78. Abdel‐Ghany M.F, M.F. Ayad, and M.M. Tadros(2018). Liquid chromatographic and spectrofluorimetric assays of empagliflozin: Applied to degradation kinetic study and content uniformity testing. Luminescence.
    View at Publisher | View at Google Scholar
  79. Ayoub B.M(2015). UPLC simultaneous determination of empagliflozin, linagliptin and metformin. RSC advances, 5(116): 95703-95709.
    View at Publisher | View at Google Scholar
  80. Ayoub B.M., S. Mowaka, E.S. Elzanfaly, N. Ashoush, M.M. Elmazar, and S.A. Mousa(2017), Pharmacokinetic Evaluation of Empagliflozin in Healthy Egyptian Volunteers Using LC-MS/MS and Comparison with Other Ethnic Populations. Scientific reports,7(1): 2583.
    View at Publisher | View at Google Scholar
  81. Godasu S. and S. Sreenivas(2017). A new validated RP-HPLC method for the determination of Metformin HCL and Empagliflozin in its bulk and pharmaceutical dosage forms. International Journal of Pharmaceutical Sciences and Research, 8(5): 2223-2232.
    View at Publisher | View at Google Scholar
  82. Padmaja N. and G. Veerabhadram(2017). A Novel Stability Indicating Rp-Uplc-Dad Method for Determination of Metformin and Empagliflozin in Bulk and Tablet Dosage form. Oriental Journal of Chemistry, 33(4): 1949-1958.
    View at Publisher | View at Google Scholar
  83. Jaiswal S.H., M. Katariya, V. Katariya, G. Karva, and K. Koshe(2017). Validated Stability Indicating Hplc Method For Determination Of Process Related Impurities In Empagliflozin Drug Substances. World journal of pharmaceutical research, 6: 8741.
    View at Publisher | View at Google Scholar
  84. Mabrouk M.M, S.M. Soliman, H.M. El-Agizy, and F.R. Mansour(2019). A UPLC/DAD method for simultaneous determination of empagliflozin and three related substances in spiked human plasma. BMC Chemistry, 13(1): 83.
    View at Publisher | View at Google Scholar
  85. Hassib S.T, E.A. Taha, E.F. Elkady, and G.H. Barakat(2019). Validated Liquid Chromatographic Method for the Determination of (Canagliflozin, Dapagliflozin or Empagliflozin) and Metformin in the Presence of (1-Cyanoguanidine). Journal of chromatographic science.
    View at Publisher | View at Google Scholar
  86. Maher H.M., A.E. Abdelrahman, N.Z. Alzoman, and H.I. Aljohar(2019). Stability-indicating capillary electrophoresis method for the simultaneous determination of metformin hydrochloride, saxagliptin hydrochloride, and dapagliflozin in pharmaceutical tablets. Journal of Liquid Chromatography & Related Technologies, 42(5-6): 161-171.
    View at Publisher | View at Google Scholar
  87. Abdallah N.A. and H.F. Ibrahim(2019). Electrochemical determination of Saxagliptin hydrochloride with MWCNTs/CuO/4′ aminobenzo-18-crown-6-ether composite modified carbon paste electrode. Microchemical Journal, , 147: 487-496.
    View at Publisher | View at Google Scholar

Clearly Auctoresonline and particularly Psychology and Mental Health Care Journal is dedicated to improving health care services for individuals and populations. The editorial boards' ability to efficiently recognize and share the global importance of health literacy with a variety of stakeholders. Auctoresonline publishing platform can be used to facilitate of optimal client-based services and should be added to health care professionals' repertoire of evidence-based health care resources.

img

Virginia E. Koenig

Journal of Clinical Cardiology and Cardiovascular Intervention The submission and review process was adequate. However I think that the publication total value should have been enlightened in early fases. Thank you for all.

img

Delcio G Silva Junior

Journal of Women Health Care and Issues By the present mail, I want to say thank to you and tour colleagues for facilitating my published article. Specially thank you for the peer review process, support from the editorial office. I appreciate positively the quality of your journal.

img

Ziemlé Clément Méda

Journal of Clinical Research and Reports I would be very delighted to submit my testimonial regarding the reviewer board and the editorial office. The reviewer board were accurate and helpful regarding any modifications for my manuscript. And the editorial office were very helpful and supportive in contacting and monitoring with any update and offering help. It was my pleasure to contribute with your promising Journal and I am looking forward for more collaboration.

img

Mina Sherif Soliman Georgy

We would like to thank the Journal of Thoracic Disease and Cardiothoracic Surgery because of the services they provided us for our articles. The peer-review process was done in a very excellent time manner, and the opinions of the reviewers helped us to improve our manuscript further. The editorial office had an outstanding correspondence with us and guided us in many ways. During a hard time of the pandemic that is affecting every one of us tremendously, the editorial office helped us make everything easier for publishing scientific work. Hope for a more scientific relationship with your Journal.

img

Layla Shojaie

The peer-review process which consisted high quality queries on the paper. I did answer six reviewers’ questions and comments before the paper was accepted. The support from the editorial office is excellent.

img

Sing-yung Wu

Journal of Neuroscience and Neurological Surgery. I had the experience of publishing a research article recently. The whole process was simple from submission to publication. The reviewers made specific and valuable recommendations and corrections that improved the quality of my publication. I strongly recommend this Journal.

img

Orlando Villarreal

Dr. Katarzyna Byczkowska My testimonial covering: "The peer review process is quick and effective. The support from the editorial office is very professional and friendly. Quality of the Clinical Cardiology and Cardiovascular Interventions is scientific and publishes ground-breaking research on cardiology that is useful for other professionals in the field.

img

Katarzyna Byczkowska

Thank you most sincerely, with regard to the support you have given in relation to the reviewing process and the processing of my article entitled "Large Cell Neuroendocrine Carcinoma of The Prostate Gland: A Review and Update" for publication in your esteemed Journal, Journal of Cancer Research and Cellular Therapeutics". The editorial team has been very supportive.

img

Anthony Kodzo-Grey Venyo

Testimony of Journal of Clinical Otorhinolaryngology: work with your Reviews has been a educational and constructive experience. The editorial office were very helpful and supportive. It was a pleasure to contribute to your Journal.

img

Pedro Marques Gomes

Dr. Bernard Terkimbi Utoo, I am happy to publish my scientific work in Journal of Women Health Care and Issues (JWHCI). The manuscript submission was seamless and peer review process was top notch. I was amazed that 4 reviewers worked on the manuscript which made it a highly technical, standard and excellent quality paper. I appreciate the format and consideration for the APC as well as the speed of publication. It is my pleasure to continue with this scientific relationship with the esteem JWHCI.

img

Bernard Terkimbi Utoo

This is an acknowledgment for peer reviewers, editorial board of Journal of Clinical Research and Reports. They show a lot of consideration for us as publishers for our research article “Evaluation of the different factors associated with side effects of COVID-19 vaccination on medical students, Mutah university, Al-Karak, Jordan”, in a very professional and easy way. This journal is one of outstanding medical journal.

img

Prof Sherif W Mansour

Dear Hao Jiang, to Journal of Nutrition and Food Processing We greatly appreciate the efficient, professional and rapid processing of our paper by your team. If there is anything else we should do, please do not hesitate to let us know. On behalf of my co-authors, we would like to express our great appreciation to editor and reviewers.

img

Hao Jiang

As an author who has recently published in the journal "Brain and Neurological Disorders". I am delighted to provide a testimonial on the peer review process, editorial office support, and the overall quality of the journal. The peer review process at Brain and Neurological Disorders is rigorous and meticulous, ensuring that only high-quality, evidence-based research is published. The reviewers are experts in their fields, and their comments and suggestions were constructive and helped improve the quality of my manuscript. The review process was timely and efficient, with clear communication from the editorial office at each stage. The support from the editorial office was exceptional throughout the entire process. The editorial staff was responsive, professional, and always willing to help. They provided valuable guidance on formatting, structure, and ethical considerations, making the submission process seamless. Moreover, they kept me informed about the status of my manuscript and provided timely updates, which made the process less stressful. The journal Brain and Neurological Disorders is of the highest quality, with a strong focus on publishing cutting-edge research in the field of neurology. The articles published in this journal are well-researched, rigorously peer-reviewed, and written by experts in the field. The journal maintains high standards, ensuring that readers are provided with the most up-to-date and reliable information on brain and neurological disorders. In conclusion, I had a wonderful experience publishing in Brain and Neurological Disorders. The peer review process was thorough, the editorial office provided exceptional support, and the journal's quality is second to none. I would highly recommend this journal to any researcher working in the field of neurology and brain disorders.

img

Dr Shiming Tang

Dear Agrippa Hilda, Journal of Neuroscience and Neurological Surgery, Editorial Coordinator, I trust this message finds you well. I want to extend my appreciation for considering my article for publication in your esteemed journal. I am pleased to provide a testimonial regarding the peer review process and the support received from your editorial office. The peer review process for my paper was carried out in a highly professional and thorough manner. The feedback and comments provided by the authors were constructive and very useful in improving the quality of the manuscript. This rigorous assessment process undoubtedly contributes to the high standards maintained by your journal.

img

Raed Mualem

International Journal of Clinical Case Reports and Reviews. I strongly recommend to consider submitting your work to this high-quality journal. The support and availability of the Editorial staff is outstanding and the review process was both efficient and rigorous.

img

Andreas Filippaios

Thank you very much for publishing my Research Article titled “Comparing Treatment Outcome Of Allergic Rhinitis Patients After Using Fluticasone Nasal Spray And Nasal Douching" in the Journal of Clinical Otorhinolaryngology. As Medical Professionals we are immensely benefited from study of various informative Articles and Papers published in this high quality Journal. I look forward to enriching my knowledge by regular study of the Journal and contribute my future work in the field of ENT through the Journal for use by the medical fraternity. The support from the Editorial office was excellent and very prompt. I also welcome the comments received from the readers of my Research Article.

img

Dr Suramya Dhamija

Dear Erica Kelsey, Editorial Coordinator of Cancer Research and Cellular Therapeutics Our team is very satisfied with the processing of our paper by your journal. That was fast, efficient, rigorous, but without unnecessary complications. We appreciated the very short time between the submission of the paper and its publication on line on your site.

img

Bruno Chauffert

I am very glad to say that the peer review process is very successful and fast and support from the Editorial Office. Therefore, I would like to continue our scientific relationship for a long time. And I especially thank you for your kindly attention towards my article. Have a good day!

img

Baheci Selen

"We recently published an article entitled “Influence of beta-Cyclodextrins upon the Degradation of Carbofuran Derivatives under Alkaline Conditions" in the Journal of “Pesticides and Biofertilizers” to show that the cyclodextrins protect the carbamates increasing their half-life time in the presence of basic conditions This will be very helpful to understand carbofuran behaviour in the analytical, agro-environmental and food areas. We greatly appreciated the interaction with the editor and the editorial team; we were particularly well accompanied during the course of the revision process, since all various steps towards publication were short and without delay".

img

Jesus Simal-Gandara

I would like to express my gratitude towards you process of article review and submission. I found this to be very fair and expedient. Your follow up has been excellent. I have many publications in national and international journal and your process has been one of the best so far. Keep up the great work.

img

Douglas Miyazaki

We are grateful for this opportunity to provide a glowing recommendation to the Journal of Psychiatry and Psychotherapy. We found that the editorial team were very supportive, helpful, kept us abreast of timelines and over all very professional in nature. The peer review process was rigorous, efficient and constructive that really enhanced our article submission. The experience with this journal remains one of our best ever and we look forward to providing future submissions in the near future.

img

Dr Griffith

I am very pleased to serve as EBM of the journal, I hope many years of my experience in stem cells can help the journal from one way or another. As we know, stem cells hold great potential for regenerative medicine, which are mostly used to promote the repair response of diseased, dysfunctional or injured tissue using stem cells or their derivatives. I think Stem Cell Research and Therapeutics International is a great platform to publish and share the understanding towards the biology and translational or clinical application of stem cells.

img

Dr Tong Ming Liu

I would like to give my testimony in the support I have got by the peer review process and to support the editorial office where they were of asset to support young author like me to be encouraged to publish their work in your respected journal and globalize and share knowledge across the globe. I really give my great gratitude to your journal and the peer review including the editorial office.

img

Husain Taha Radhi

I am delighted to publish our manuscript entitled "A Perspective on Cocaine Induced Stroke - Its Mechanisms and Management" in the Journal of Neuroscience and Neurological Surgery. The peer review process, support from the editorial office, and quality of the journal are excellent. The manuscripts published are of high quality and of excellent scientific value. I recommend this journal very much to colleagues.

img

S Munshi

Dr.Tania Muñoz, My experience as researcher and author of a review article in The Journal Clinical Cardiology and Interventions has been very enriching and stimulating. The editorial team is excellent, performs its work with absolute responsibility and delivery. They are proactive, dynamic and receptive to all proposals. Supporting at all times the vast universe of authors who choose them as an option for publication. The team of review specialists, members of the editorial board, are brilliant professionals, with remarkable performance in medical research and scientific methodology. Together they form a frontline team that consolidates the JCCI as a magnificent option for the publication and review of high-level medical articles and broad collective interest. I am honored to be able to share my review article and open to receive all your comments.

img

Tania Munoz

“The peer review process of JPMHC is quick and effective. Authors are benefited by good and professional reviewers with huge experience in the field of psychology and mental health. The support from the editorial office is very professional. People to contact to are friendly and happy to help and assist any query authors might have. Quality of the Journal is scientific and publishes ground-breaking research on mental health that is useful for other professionals in the field”.

img

George Varvatsoulias

Dear editorial department: On behalf of our team, I hereby certify the reliability and superiority of the International Journal of Clinical Case Reports and Reviews in the peer review process, editorial support, and journal quality. Firstly, the peer review process of the International Journal of Clinical Case Reports and Reviews is rigorous, fair, transparent, fast, and of high quality. The editorial department invites experts from relevant fields as anonymous reviewers to review all submitted manuscripts. These experts have rich academic backgrounds and experience, and can accurately evaluate the academic quality, originality, and suitability of manuscripts. The editorial department is committed to ensuring the rigor of the peer review process, while also making every effort to ensure a fast review cycle to meet the needs of authors and the academic community. Secondly, the editorial team of the International Journal of Clinical Case Reports and Reviews is composed of a group of senior scholars and professionals with rich experience and professional knowledge in related fields. The editorial department is committed to assisting authors in improving their manuscripts, ensuring their academic accuracy, clarity, and completeness. Editors actively collaborate with authors, providing useful suggestions and feedback to promote the improvement and development of the manuscript. We believe that the support of the editorial department is one of the key factors in ensuring the quality of the journal. Finally, the International Journal of Clinical Case Reports and Reviews is renowned for its high- quality articles and strict academic standards. The editorial department is committed to publishing innovative and academically valuable research results to promote the development and progress of related fields. The International Journal of Clinical Case Reports and Reviews is reasonably priced and ensures excellent service and quality ratio, allowing authors to obtain high-level academic publishing opportunities in an affordable manner. I hereby solemnly declare that the International Journal of Clinical Case Reports and Reviews has a high level of credibility and superiority in terms of peer review process, editorial support, reasonable fees, and journal quality. Sincerely, Rui Tao.

img

Rui Tao

Clinical Cardiology and Cardiovascular Interventions I testity the covering of the peer review process, support from the editorial office, and quality of the journal.

img

Khurram Arshad

Clinical Cardiology and Cardiovascular Interventions, we deeply appreciate the interest shown in our work and its publication. It has been a true pleasure to collaborate with you. The peer review process, as well as the support provided by the editorial office, have been exceptional, and the quality of the journal is very high, which was a determining factor in our decision to publish with you.

img

Gomez Barriga Maria Dolores

The peer reviewers process is quick and effective, the supports from editorial office is excellent, the quality of journal is high. I would like to collabroate with Internatioanl journal of Clinical Case Reports and Reviews journal clinically in the future time.

img

Lin Shaw Chin

Clinical Cardiology and Cardiovascular Interventions, I would like to express my sincerest gratitude for the trust placed in our team for the publication in your journal. It has been a true pleasure to collaborate with you on this project. I am pleased to inform you that both the peer review process and the attention from the editorial coordination have been excellent. Your team has worked with dedication and professionalism to ensure that your publication meets the highest standards of quality. We are confident that this collaboration will result in mutual success, and we are eager to see the fruits of this shared effort.

img

Maria Dolores Gomez Barriga

Dear Dr. Jessica Magne, Editorial Coordinator 0f Clinical Cardiology and Cardiovascular Interventions, I hope this message finds you well. I want to express my utmost gratitude for your excellent work and for the dedication and speed in the publication process of my article titled "Navigating Innovation: Qualitative Insights on Using Technology for Health Education in Acute Coronary Syndrome Patients." I am very satisfied with the peer review process, the support from the editorial office, and the quality of the journal. I hope we can maintain our scientific relationship in the long term.

img

Dr Maria Dolores Gomez Barriga

Dear Monica Gissare, - Editorial Coordinator of Nutrition and Food Processing. ¨My testimony with you is truly professional, with a positive response regarding the follow-up of the article and its review, you took into account my qualities and the importance of the topic¨.

img

Dr Maria Regina Penchyna Nieto

Dear Dr. Jessica Magne, Editorial Coordinator 0f Clinical Cardiology and Cardiovascular Interventions, The review process for the article “The Handling of Anti-aggregants and Anticoagulants in the Oncologic Heart Patient Submitted to Surgery” was extremely rigorous and detailed. From the initial submission to the final acceptance, the editorial team at the “Journal of Clinical Cardiology and Cardiovascular Interventions” demonstrated a high level of professionalism and dedication. The reviewers provided constructive and detailed feedback, which was essential for improving the quality of our work. Communication was always clear and efficient, ensuring that all our questions were promptly addressed. The quality of the “Journal of Clinical Cardiology and Cardiovascular Interventions” is undeniable. It is a peer-reviewed, open-access publication dedicated exclusively to disseminating high-quality research in the field of clinical cardiology and cardiovascular interventions. The journal's impact factor is currently under evaluation, and it is indexed in reputable databases, which further reinforces its credibility and relevance in the scientific field. I highly recommend this journal to researchers looking for a reputable platform to publish their studies.

img

Dr Marcelo Flavio Gomes Jardim Filho

Dear Editorial Coordinator of the Journal of Nutrition and Food Processing! "I would like to thank the Journal of Nutrition and Food Processing for including and publishing my article. The peer review process was very quick, movement and precise. The Editorial Board has done an extremely conscientious job with much help, valuable comments and advices. I find the journal very valuable from a professional point of view, thank you very much for allowing me to be part of it and I would like to participate in the future!”

img

Zsuzsanna Bene

Dealing with The Journal of Neurology and Neurological Surgery was very smooth and comprehensive. The office staff took time to address my needs and the response from editors and the office was prompt and fair. I certainly hope to publish with this journal again.Their professionalism is apparent and more than satisfactory. Susan Weiner

img

Dr Susan Weiner

My Testimonial Covering as fellowing: Lin-Show Chin. The peer reviewers process is quick and effective, the supports from editorial office is excellent, the quality of journal is high. I would like to collabroate with Internatioanl journal of Clinical Case Reports and Reviews.

img

Lin-Show Chin

My experience publishing in Psychology and Mental Health Care was exceptional. The peer review process was rigorous and constructive, with reviewers providing valuable insights that helped enhance the quality of our work. The editorial team was highly supportive and responsive, making the submission process smooth and efficient. The journal's commitment to high standards and academic rigor makes it a respected platform for quality research. I am grateful for the opportunity to publish in such a reputable journal.

img

Sonila Qirko

My experience publishing in International Journal of Clinical Case Reports and Reviews was exceptional. I Come forth to Provide a Testimonial Covering the Peer Review Process and the editorial office for the Professional and Impartial Evaluation of the Manuscript.

img

Luiz Sellmann

I would like to offer my testimony in the support. I have received through the peer review process and support the editorial office where they are to support young authors like me, encourage them to publish their work in your esteemed journals, and globalize and share knowledge globally. I really appreciate your journal, peer review, and editorial office.

img

Zhao Jia

Dear Agrippa Hilda- Editorial Coordinator of Journal of Neuroscience and Neurological Surgery, "The peer review process was very quick and of high quality, which can also be seen in the articles in the journal. The collaboration with the editorial office was very good."

img

Thomas Urban

I would like to express my sincere gratitude for the support and efficiency provided by the editorial office throughout the publication process of my article, “Delayed Vulvar Metastases from Rectal Carcinoma: A Case Report.” I greatly appreciate the assistance and guidance I received from your team, which made the entire process smooth and efficient. The peer review process was thorough and constructive, contributing to the overall quality of the final article. I am very grateful for the high level of professionalism and commitment shown by the editorial staff, and I look forward to maintaining a long-term collaboration with the International Journal of Clinical Case Reports and Reviews.

img

Cristina Berriozabal

To Dear Erin Aust, I would like to express my heartfelt appreciation for the opportunity to have my work published in this esteemed journal. The entire publication process was smooth and well-organized, and I am extremely satisfied with the final result. The Editorial Team demonstrated the utmost professionalism, providing prompt and insightful feedback throughout the review process. Their clear communication and constructive suggestions were invaluable in enhancing my manuscript, and their meticulous attention to detail and dedication to quality are truly commendable. Additionally, the support from the Editorial Office was exceptional. From the initial submission to the final publication, I was guided through every step of the process with great care and professionalism. The team's responsiveness and assistance made the entire experience both easy and stress-free. I am also deeply impressed by the quality and reputation of the journal. It is an honor to have my research featured in such a respected publication, and I am confident that it will make a meaningful contribution to the field.

img

Dr Tewodros Kassahun Tarekegn

"I am grateful for the opportunity of contributing to [International Journal of Clinical Case Reports and Reviews] and for the rigorous review process that enhances the quality of research published in your esteemed journal. I sincerely appreciate the time and effort of your team who have dedicatedly helped me in improvising changes and modifying my manuscript. The insightful comments and constructive feedback provided have been invaluable in refining and strengthening my work".

img

Dr Shweta Tiwari

I thank the ‘Journal of Clinical Research and Reports’ for accepting this article for publication. This is a rigorously peer reviewed journal which is on all major global scientific data bases. I note the review process was prompt, thorough and professionally critical. It gave us an insight into a number of important scientific/statistical issues. The review prompted us to review the relevant literature again and look at the limitations of the study. The peer reviewers were open, clear in the instructions and the editorial team was very prompt in their communication. This journal certainly publishes quality research articles. I would recommend the journal for any future publications.

img

Dr Farooq Wandroo

Dear Jessica Magne, with gratitude for the joint work. Fast process of receiving and processing the submitted scientific materials in “Clinical Cardiology and Cardiovascular Interventions”. High level of competence of the editors with clear and correct recommendations and ideas for enriching the article.

img

Dr Anyuta Ivanova

We found the peer review process quick and positive in its input. The support from the editorial officer has been very agile, always with the intention of improving the article and taking into account our subsequent corrections.

img

Dr David Vinyes