Pathogenic bacteria antimicrobial resistance consider a significant danger to human health, so many new antibiotic-free approaches are being developed. “Non-Thermal Treatments Ultraviolet (UV) Light Radiation” used for industrial sterilization, was considered the third and fourth technology in kill pathogenic bacteria. The aim was experiment with UV Light Radiation their effect on pathogenic bacteria in order to reduce the use of antibiotics and reduce their effect on patients. The UV Light Radiation was used in elimination pathogenic bacteria in less than ten minutes in order to maintain public health and reduce the occurrence of infectious diseases. The understudy pathogenic bacteria were collected from “Private Laboratory”, included Staphylococcous aureus, Listeria monocytogenes, Salmonella typhi, Pseudomonas aeruginosa, Escherichia coli, and Klebsiella pneumoniae. The LED equipment was treated the bacterial suspensions (~5 log CFU/mL) in wavelengths (280+300)nm for (1-7second). The pathogenic bacteria passed via bacterial membrane device, then elimination percentage were done. Statistical analysis was treated by IBM SPSS Statistics Software Version 21. The results presented an elimination percentage of pathogenic bacteria, the high significant antipathogenic bacterial effects were (P<0.001). The results observed the effect of UV Light Radiation which eliminated the pathogenic bacteria were Staphylococcous aureus, Escherichia coli and Klebsiella pneumoniae within six minutes. Listeria monocytogenes, Salmonella typhi, also Pseudomonas aeruginosa were eliminated at the seventh minute by UV Light Radiation. It was concluded that through experiment action of UV Light Radiation was evident within less than ten minutes to eliminate the pathogenic bacteria which cause infectious diseases. This was one of the ways to maintain public health and eliminate pathogenic bacterial cause infection diseases that affect public health. It was recommend that through experiment, the use of UV Light Radiation in hospitals, clinics, sanitary, and food production factories and others. It could be a quickly eliminate pathogenic bacteria and thus maintain public health and advance sterilization methods.

Antimicrobial resistance pathogenic bacteria is a human health significant threat, new antibiotics were developed resulting in the interface of physics factors as “Non-Thermal TreatmentsUltraviolet (UV) Light Radiation” are increasing used for industrial sterilization. It’s reasoned the third and fourth application with higher technical application in kill the pathogenic bacteria [1]. It was alternate to thermal saving sterilization in gentle temperatures [2]. It showed different antipathogenic bacteria effects where using 280/300nm, the most effective treatment was with 4Dt value of 5 s observed in the bacterial suspension of 5 log CFU/mL. That explained dynamics of the pathogenic bacteria inactivation and UV Light Radiation procedure of industrial level [3]. It revealed 4log action of pathogenic bacterial strains, certain UV-tolerant Pseudomonas byphoto-lyases [4]. Xenex Germ-Zapping Robot TM, high-intensity flashing light (200–320nm) that chenal done the bacteria and bacterial spores cell wall, effort had harm to DNA, RNA, and proteins by photo-hydration, photo-splitting, photo-crosslinking, and photo-dimerization [5]. It had action be upon quiescence of DNA reproduction on cells are in a inactive form, put off death by precaution their basal constituent, that straight treaty pathogenic bacterial DNA [4]. It had straight impacts DNA by the arrangement nitrogen bases, also mutations arising in different genes pertaining to DNA repair and replication [6]. It and Oxidation Processes UV-Based Advanced uses sensual energy, control spoilage microorganisms used (220-280) nm, caused DNA physical change and suppress bacterial reproduction by arrangement of protein bases, artifact DNA reproduction, leading to cell death. The bactericidal acts mainly be on dose (J/m2) [7]. The influence to berth or sub-lethal doses was for elimination of cells within pathogenic bacterial biofilms [8]. 

The aim was to experiment with UV Light Radiation methods other than antibiotics and their effect on the pathogenic bacteria to decrease the use of antibiotics and reduce their effect on patients. As well as it reduce the pathogenic bacteria mutation and create mutated pathogenic bacterial strains resistant to antibiotics, they need new antibiotics. UV Light Radiation was used in elimination the pathogenic bacteria in less than ten minutes to maintain public health and decrease occurrence of infectious diseases caused by pathogenic bacteria.

• Understudy Pathogenic Bacteria: They were included Staphylococcous aureus, Listeria monocytogenes, Salmonella typhi, Pseudomonas aeruginosa, Escherichia coli, and Klebsiella pneumoniae. They were inoculated on Mueller Hinton Agar (Oxoid, Basingstoke, UK) for 24 hour. The colonies were transferred on Mueller Hinton Broth (CM0405, Oxoid) to make 0.5 McFarland cloudiness (~5 log CFU/mL) [9].
• LED Equipment: It was (PearlLab Beam, Aquisense technologies, NC, USA), it was treated samples with wavelengths (280+300)nm for treatment times (1, 2, 3, 4, 5, 6, and 7 second), and Intensity/influence rate was 0.112W/cm2 [3].
• UV Light Radiation Treated Pathogenic Bacteria: The bacterial suspensions 10 mL were displaced in glassful Petri-dishes (without cover) with degree of ~6 mm and volume 24 cm3. Then Petri-dishes were centrally set in LED chamber spacing 5cm of origin [3].
• Bacterial Membrane Device:After treated they were passed via bacterial membrane device. The filters were transited to Mueller-Hinton Agar (Oxoid, Basingstoke, UK), were incubated 37oC for 24 hour. The elimination percents were made by the Equation [Colony Number/300 X 100-100] [10].
• Statistical Analysis: All results was treated by IBM SPSS Statistics software version 21 (IBM, Armonk, NY, United States) [11].

• Elimination percents of pathogenic bacteria treated with UV Light Radiation:

Table 1 and Graph 1 presented an elimination percents of pathogenic bacteria treated with UV Light Radiation. That were with the “Wavelengths (280+300) nm, treatment times were from (1-7second)”. It was displayed the high significant antipathogenic bacterial effects were (P<0>Staphylococcous aureus, Escherichia coli and Klebsiella pneumoniae were eliminated within six minutes. Listeria monocytogenes, Salmonella typhi, and Pseudomonas aeruginosa were eliminated at the seventh minute by it (Table 1, Graph 1). Antimicrobial resistance pathogenic bacteria is a human health significant threat. Physics factors as “Non-Thermal TreatmentsUltraviolet (UV) Light Radiation” are increasingly used for industrial sterilization. UV Light radiation reasoned the third and fourth application application in kill pathogenic bacteria [1], it has saving sterilization [2], it had antipathogenic bacteria effects of 280/300nm [3]. UV-tolerant Pseudomonas by photo-lyases [4]. Xenex Germ-Zapping Robot TM, high-intensity flashing light (200–320nm) harm to DNA, and RNA, by photo-hydration, photo splitting, photo-crosslinking, and photo-dimerization [5]. It is on DNA quiescence reproduction on cells inactive form, put off death by precaution their basal constituent, straight treaty pathogenic bacterial DNA [4]. It straight impacts DNA, mutations and replication [6-7]. So for elimination pathogenic bacterial biofilms [8]. The pathogenic bacterial cells treated by UV Light Radiation were affect cell growth and division leads to cell failure and death, lading to pathogenic bacterial elimination [12]. It was harmless to humans, as non-antibiotics UV Light Radiation. It eliminated the pathogenic bacteria that affecting public health and leave shadow on diseased individuals from antibiotics. As a result of their use and pathogenic bacterial mutation [13]. UV Light Radiation can be used to maintain public health and avoid losses. As well as sterilization of sanitary tools, and the absence of transmission of pathogenic bacteria cause infectious diseases. It reducing food poisoning, maintains public health and protection from pathogenic bacterial causes and the transmission of diseases between individuals [14].

Table 1: Elimination percents of pathogenic bacteria treated with UV Light Radiation

Graph 1: Elimination percents of pathogenic bacteria treated with UV Light Radiation

It was concluded that through experiment effect of UV Light Radiation was evident within less than ten minutes to eliminate pathogenic bacteria cause infectious diseases. This was one of the ways to maintain public health and eliminate pathogenic bacterial causes infections diseases that affect public health.

It was recommend that through experiment, the use of UV Light Radiation in hospitals, clinics, sanitary, and food production factories etc…… That it was a quickly eliminate pathogenic bacteria and thus maintain public health and advance sterilization methods.

The authors would passed their dear appreciation to “Private Laboratory”, and technicians assisted in the experiment.