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Research | DOI: https://doi.org/10.31579/2768-0487/190
Assistant Professor, Department of Animal Nutrition and Biochemistry, Sumitra Research Institute, Gujarat, India.
*Corresponding Author: Alagbe John Olujimi., Assistant Professor, Department of Animal Nutrition and Biochemistry, Sumitra Research Institute, Gujarat, India.
Citation: Alagbe J. Olujimi (2025), Investigating the Effect of Dietary Supplementation of Ficus Exasperata Oil on The Growth Performance, Apparent Digestibility and Nitrogen Utilization of Weaner Rabbits, Journal of Clinical and Laboratory Research, 8(6); DOI:10.31579/2768-0487/190
Copyright: © 2025, Alagbe John Olujimi. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Received: 03 November 2025 | Accepted: 14 November 2025 | Published: 08 December 2025
Keywords: ficus exasperata oil; growth; nutrient retention; nutrient utilization; rabbits
This research was carried out to investigate the effect of dietary supplementation of Ficus exasperata oil on the growth performance, apparent digestibility and nitrogen utilization of weaner rabbits. Forty healthy weaned male rabbits of mixed breed with a mean body weight of 507 g (± 1.80 g) and an average age of 4 months were randomly assigned into four treatments (n=10 rabbits/treatment). Experimental treatments included: 1- control diet (without essential oil), 2- basal diet supplemented with 250 mg Ficus exasperata oil kg DM per day, 3- basal diet supplemented with 500 mg Ficus exasperata oil kg DM per day and 4- basal diet supplemented with 750 mg Ficus exasperata oil/ kg DM daily. Basal diet was compounded to meet the standard nutritional requirements for rabbits according to NRC recommendation. The experimental period was 60 days excluding 14 days’ acclimatization period. A completely randomized design was adopted and animals received fresh water ad libitum. Ficus exasperata oil supplementation significantly increased (p˂0.05) average daily weight gain, average daily feed intake as well as feed conversion ratio. Similarly, dietary supplementation of Ficus exasperata oil enhanced (p< 0.05) the apparent digestibilities of dry matter, organic matter, ether extract, crude protein and crude fibre. Ficus exasperata oil supplementation decreased faecal and urinary nitrogen and increased the concentrations (p < 0.05) of nitrogen intake and nitrogen retention. In conclusion, Ficus exasperata oil dietary supplementation at 750 mg/kg DM daily improved growth performance, nutrient digestibility and nitrogen retention of weaner rabbits without causing any deleterious effect on their health status.
Antibiotic residues in animal products are considered as potential health hazard for human consumption due to developing multi-drug resistance [1, 15]. In recent years, the use of essential oils from medicinal plants has emerged as one of the most sustainable strategy to enhance animal performance particularly in reducing the use of antibiotics [2]. This is because plant extracts are generally regarded as safe, effective, do not produce environmental pollution and has no withdrawal period [15, 22]. Herbs like Ficus exasperate have several potentials and could be used as alternative to antibiotics due to their abundant phyto-components [3]. Ficus exasperate commonly known as sand paper fig is a deciduous small plant belonging to the family Moraceae and order Urticales [3]. The plant is widely distributed in Africa, Australia, America and some parts of Asia including India [4]. Phytochemical studies on the various parts of the plant have revealed the presence of flavonoids, saponins, tannins, phenolics, glycosides, saponins, carbonyl compounds and alcohols [4]. In recent work [25], Ficus exasperate essential oil have been found to contained; α-Pinene (2.2 %), p-Cymene (11.4 %), 1, 8-cineole (13.8 %), β-Caryophyllene (3.80 %), α-Ionone (2.90 %), Neryl acetone (4.2 %), β-Ionone (7.50 %), Caryophyllene oxide (5.4%), 6,10,14-Trimethyl-2-pentadecanone (7.00 %), 9-Octadecenoic acid (2.90 %), Cyclooctasulfur (6.30 %) and phytol (13.70 %). These compounds have numerous pharmacological activities such as, anti-inflammatory, antioxidant, anti-tumor, antidiabetic, hypolipidemic, hypoglycemic, anti-arthritic, anticonvulsant, immuno-modulatory, gastro-protective, antiplatelet, cytotoxicity, angiogenic, antineoplasic, antiviral, sedative, muscle relaxant, cytotoxic, antiviral, insecticidal, cardiotonic, analgesic, inhibition of lipid peroxidation, fungicidal, antiprotozoal, antimalarial, and anti-rheumatic, antifertility, antinociceptive, amongst others [6,8]. Aqueous and ethanolic extract from the leaves, stem and roots of Ficus exasperate have been reported to inhibit the activities of Escherichia coli, Staphyllococcus spp, Salmonella spp, Klebsiellia spp, Aspergillus niger, Candida albicans, Penicillium notatum and Rhizopus stolon [7,9]. It has also been used to traditionally treat fever, catarrh, allergic eruptions, somniferous, nervousness, cough, diabetes, asthma, bronchitis, prostrate disease, tumor, gastro-intestinal disorder, skin infections, snake bite amongst others [10]. It has been well-documented [22] that dietary inclusion of essential oils could improve rabbit performance by increasing digestive enzyme secretion, lowering the number of harmful bacteria in the digestive tract, modulating intestinal morphology functions, and positively affecting productivity, blood metabolites, and immunological and antioxidant status of animals. For instance, extracts from herbs such as Rauvolfia Vomitoria extract at 3 mL per litre has been demonstrated to promote growth and scavenge the activities of free radicals due to the active ingredients (phenols, tannins, alkaloids, saponins and flavonoids) of these herbs possessing antioxidants effect [23]. However, there is little or no information on the dietary supplementation of Ficus exasperate on the performance of rabbits. Evaluating its effect on rabbits will help to establish an optimum level, promote sustainability and help to address the increasing cases of antimicrobial resistance.
Study Area
The study was carried out at the Rabbit section, Gandhi College of Agriculture located at Rajasthan, India. Experiment animals were taken care of according to the Indian Council guidelines of Animal Care. Laboratory analysis on the chemical composition of experimental diet, fecal droppings and chemical composition of Ficus exasperata oil was carried out at Sumitra Research Institute, Gujarat India using standard procedures according to Association of Analytical Chemist [11].
Ficus exasperata oil and Gas Chromatography-Mass Spectrometry analysis
Ficus exasperata oil was sourced from Singh Unique Company in Rajasthan, India. The oil was extraction by hydrodistillation method according to the package insert. 200 mL of purchased Ficus exasperata oil was sent to the Biochemistry Department, Sumitra Research Institute in Gujarat for proper identification of their bioactive components by Gas Chromatography – Mass Spectrometry (GC-MS). GC-MS analysis was done using Thermo Specific TSQ Quadrupole GC-MS system (Belgium). The kit was equipped with fused silica capillary column (35 m x 0.30 mm, film thickness 0.20 μm). Analytical conditions of the GC-unit were: Oven temperature: 65 ℃, with 2 minutes’ initial hold, and then to 250 ℃ at 6 ℃/min, with final hold time of 10 minutes; helium was used as carrier gas at a flow rate of 1 mL/min. The MS chamber was maintained at a EI mode with ionization voltage 70 eV and ion source temperature, 220 ℃ to maintain precision in results. Percentage composition of each constituent was calculated by integration of the GC peak areas. Retention indices were determined with reference to a homologous series of normal alkanes analyzed under the same conditions.
Rabbits and management
Forty healthy weaned male rabbits of mixed breed with a mean body weight of 507 g (± 1.80 g) and an average age of 4 months were purchased from a reputable breeding farm in Rajasthan, India. Animals were reared in a specially constructed battery cage which was properly disinfected two weeks before the commencement of the study. On arrival, rabbits were quarantined for 14 days and dewormed with Wormcap (Albendazole 2.5 % Amit Pharmaceutical Company, Gujarat) and fed basal diet which meet the standard nutritional requirements for rabbits according to NRC recommendation [12]. After the acclimatization period, rabbits were weighed and randomly allocated to four dietary treatments based on live weights (n=10 rabbits/treatment). Experimental treatments included: 1- control diet (without essential oil), 2- basal diet supplemented with 250 mg Ficus exasperata oil kg DM per day, 3- basal diet supplemented with 500 mg Ficus exasperata oil kg DM per day and 4- basal diet supplemented with 750 mg Ficus exasperata oil/ kg DM per day. Animals were fed thrice daily and offered equal amount of feed at 7:30, 12:30 and 17:30. To ensure that animals take a full dose of Ficus exasperata oil, supplement, the oil was mixed in 200 g total diet DM to each rabbits before the first feeding. Fresh water was supplied unrestricted throughout the period of the experiment. A completely randomized design was adopted and the duration of the experiment is 60 days. Composition of experimental diet and chemical composition of Ficus exasperata oil is presented in Table 1 and Table 2 respectively. Animals were weighed weekly during the experimental period using digital sensitive scale, feed intake was estimated as the difference between the feed offered and refusal from the previous day feeding. Average body weight gain was calculated as the difference between the average initial body weight and the average final body weight. Average daily weight gain and average daily feed intake was calculated as body weight gain and total feed intake divided by the experimental period respectively. Feed conversion ratio was calculated by dividing average daily feed intake by average daily weight gain.
Nutrient apparent digestibility and nitrogen utilization trial
At the end of the experiment, five rabbits were randomly selected per treatment for the seven days’ digestibility trial. Animals were kept in a metabolic cage (to separate their fecal dropping from urine) and allowed to acclimatize for two days while the remaining five days is for data collection. Internal indigestibility marker was mixed with feed supplied to each animals, fecal droppings and urine from each animal were collected separately every morning before feeding and were weighed and recorded daily for five days. Each day’s total faecal output was weighed and about 10% of it was saved, oven dried at 60℃ for 48 hours and coefficients of apparent digestion was calculated. The urine output also measured daily for each rabbit and kept in an airtight sample bottle containing a few drops of concentrated H2SO4 used to prevent volatilization loss of nitrogen. About 10% of the daily urinary output was saved over the 5 days’ collection period for each rabbit. Proximate composition of faecal droppings and experimental diet was carried out according to the procedure outlined by [13]
Apparent nutrient digestibility (%) = Nutrient intake (DM) - Nutrient in faeces (DM) x 100
Nutrient Intake (DM)
Nitrogen utilisation was estimated as:
N absorbed = Nitrogen intake - Faecal N
N balance or retained (%) = Nitrogen intake – faecal N – urinary N
N retention (as the % on N absorbed) = N absorbed × 100
N intake
N retention (expressed as the % of N intake) = N retained × 100
N intake
Concentrations (% DM) of organic matter (OM) = 100 – ash
All data collected were subjected to a one-way analysis of variance (ANOVA). Means showing significant differences were separated using Duncan’s Multiple Range Test. The SPSS (version 21) statistical package was used for all statistical analysis.
| S/N | Compounds | Retention time (min) | Percentage Area |
| 1 | β-caryophyllene | 6.18 | 12.95 |
| 2 | δ-cadinene | 6.57 | 9.35 |
| 3 | hexadecanoic acid | 7.01 | 15.32 |
| 4 | β-Linalol | 7.22 | 1.46 |
| 5 | Methyl Nonanoate | 7.69 | 0.6 |
| 6 | Cis-7-Decen-1-ol | 8.08 | 1.73 |
| 7 | m-Xylene | 8.55 | 8.75 |
| 8 | D-Limonene | 9.08 | 0.81 |
| 9 | 4,6-Dimethyldodecane | 9.26 | 0.17 |
| 10 | Cyclododecyl Ethanone | 9.71 | 0.33 |
| 11 | Cis-9-Tetradecenol | 10.04 | 0.49 |
| 12 | Cis-9,17-Octadecadiene | 10.63 | 0.61 |
| 13 | Squalene | 10.92 | 1.33 |
| 14 | α-Terpinolene | 11.36 | 1.78 |
| 15 | Trans-3(10)-Caren-2-ol | 11.55 | 0.12 |
| 16 | 1-Octen-3-ol | 12.09 | 0.04 |
| 17 | Bicyclo [10,6,0]Octadeca-1(12),15- diene | 12.56 | 0.11 |
| 18 | α-Bergamotene | 12.79 | 2.57 |
| 19 | Phytol | 13.02 | 27.07 |
| 20 | Isopropyl Myristate | 13.86 | 0.66 |
| 21 | Humulane-1,6-dien-3-ol | 14.05 | 1.24 |
| 22 | Epiglobulol | 14.65 | 0.02 |
| 23 | α-Glyceryl Linolenate | 14.87 | 0.71 |
| 24 | Total | - | 88.22 |
| 25 | Number of compounds | - | 23 |
| 26 | Monoterpenes | - | 1.85 |
| 27 | Sesquiterpenes | - | 59.20 |
| 28 | Diterpenes | - | 13.76 |
| 29 | Triterpenes | - | 3.91 |
| 30 | Non-terpenes | - | 9.50 |
Table 1: Compounds identified in Ficus exasperata oil by GC-MS.
| Components | Quantity (% DM) |
| Maize (9.0 % CP) | 30.00 |
| Corn barn (11% CP) | 20.00 |
| Palm kernel meal | 20.00 |
| Groundnut cake (39 % CP) | 13.50 |
| Soya bean meal (48 % CP) | 12.00 |
| Bone meal | |
| Mineral/Vitamin Premix | 2.00 |
| Bone meal | 4.00 |
| Limestone | 2.00 |
| Salt | 1.00 |
| Total | 100.0 |
| Chemical composition (% DM) | |
| Dry matter | 92.00 |
| Organic matter | 92.50 |
| Crude protein | 14.70 |
| Ether extract | 3.80 |
| Ash | 7.50 |
| Energy (Kcal/kg) | 2691.8 |
Table 2: Ingredient and chemical composition of basal diet (% DM).
2.5 kg Growers premix contained: 15,000 I.U. vitamin A; 8000 mg vitamin B1; 3000 I.U. vitamin D3; 60.0 mg vitamin E; 15 mg Choline; 0.96 mg Cobalt; 2.00 mg I; 50 manganese Mn; 0.50 mg Selenium; 250 mg.
| Variables | T1 | T2 | T3 | T4 | SEM |
| Average initial body weight (g/rabbit) | 508.7 | 507.6 | 507.0 | 506.9 | 0.08 |
| Average final body weight (g/rabbit) | 1997.8c | 2200.6b | 2298.1b | 2355.8a | 41.29 |
| Average body weight gain (g/rabbit) | 1489.1c | 1693.0b | 1791.1b | 1848.9a | 20.85 |
| Average daily weight gain (g/rabbit) | 24.81c | 28.22b | 29.85b | 39.26a | 0.06 |
| Average total feed intake (g/rabbit) | 6200.1c | 6500.3b | 6511.1b | 6800.2a | 95.07 |
| Average daily feed intake (g/rabbit | 103.34c | 108.34b | 108.51b | 113.3a | 1.22 |
| Feed conversion ratio | 4.20a | 4.00b | 4.00b | 3.10c | 0.02 |
Table 3: Growth performance of weaned rabbits fed diet supplemented with Ficus exasperata oil.
,a,b,c: Means within a row with different superscripts are significantly different (p˂0.05); T1: basal diet without oil; T2 T3 and T4: basal diet with supplemented with 250 mg, 500 mg and 750 mg Ficus exasperata oil per kg DM feed daily.
| Variables (%) | T1 | T2 | T3 | T4 | SEM |
| Dry matter | 73.66c | 80.92b | 81.40b | 86.95a | 0.19 |
| Organic matter | 79.43c | 81.87b | 81.92b | 85.67a | 0.10 |
| Crude protein | 68.40c | 72.15b | 72.55b | 78.94a | 0.07 |
| Crude fibre | 59.95b | 63.74a | 65.61a | 67.09a | 0.05 |
| Ether extract | 48.17c | 55.91b | 59.08b | 60.80a | 0.02 |
Table 4: Apparent digestibility of weaned rabbits fed diet supplemented with Ficus exasperata oil.
,a,b,c: Means within a row with different superscripts are significantly different (p˂0.05); T1: basal diet without oil; T2 T3 and T4: basal diet with supplemented with 250 mg, 500 mg and 750 mg Ficus exasperata oil per kg DM feed daily.
| Variables (%) | T1 | T2 | T3 | T4 | SEM |
| Nitrogen Intake | 9.55c | 12.76b | 12.85b | 15.77a | 0.02 |
| Faecal Nitrogen | 6.90a | 4.05b | 3.96b | 2.95c | 0.01 |
| Urinary Nitrogen | 2.59a | 1.28b | 1.21b | 1.05c | 0.01 |
| Nitrogen retention | 43.89c | 50.02b | 50.10b | 56.17a | 0.09 |
Table 4: Apparent digestibility of weaned rabbits fed diet supplemented with Ficus exasperata oil.
,a,b,c: Means within a row with different superscripts are significantly different (p˂0.05); T1: basal diet without oil; T2 T3 and T4: basal diet with supplemented with 250 mg, 500 mg and 750 mg Ficus exasperata oil per kg DM feed daily
In Table 1 compounds identified in Ficus exasperata oil by GC-MS reveals the prevalence of phytol (27.07 %), hexadecanoic acid (15.32 %), β-caryophyllene (12.95 %), δ-cadinene (9.32 %), xylene (8.75 %) as major bioactive compounds. Methyl Nonanoate (0.61 %), D-Limonene (0.81 %), Cis-9-Tetradecenol (0.49 %), 4,6-Dimethyldodecane (0.17 %), 1-Octen-3-ol (0.04 %) and Bicyclo [10,6,0] Octadeca-1(12),15- diene (0.11 %) amongst others were part of the minor compounds. Furthermore, on analysis of the class of compounds present, the essential oils from Ficus exasperata leaf were dominated by sesquiterpenes (59.20 %) followed by diterpenes (13.76 %), non-terpenes (9.50 %), triterpenes (3.91 %) and monoterpenes (1.85 %). All these compounds have numerous therapeutic activities, anti-inflammatory, anti-tumor [14], antioxidant, antifungal, immuno-modulatory [15], gastro-protective [16], cardio-protective, anti-helminthic [17], antiviral [18], antidiabetic [19], anti-cancer, antidiarrheal [20], hypo-lipidemic, hepato-protective [21] and dermato-protective [22]. It can also be used in folk medication in the treatment of cough, gastro-intestinal disorder, sexually transmitted infections, skin infection, snake bite, wound, pyrexia, malaria, ulcer amongst others [23, 24]. Result obtained in this study is in agreement with the reports of [25]. Growth performance of weaned rabbits fed diet supplemented with Ficus exasperata oil (Table 3). Average daily weight gain was lower in treatment 1 [T1] (24.81 g) than in T2 (28.22 g), T3 (29.85) and T4 (39.26 g) (p˂0.05). This result suggests that Ficus exasperata oil has an inhibitory effect against pathogenic organisms exerting beneficial effects on the gastrointestinal tract by increasing the activities of digestive enzymes translating to better weight gain especially when it is supplemented in the diet of rabbits between 250 – 750 mg/kg DM daily. The result obtained is in agreement with the reports of [26] who supplemented Moringa oleifera and Rosmarius officinalis leaves was supplemented in the diet of growing rabbits. [27] recorded a higher body weight in weaner rabbits fed diet supplemented with 200 mg of Allium sativum and Curcuma longa oil. Average daily feed intake in T2 (108.34 g), T3 (108.51 g) and T4 (113.30 g) were similar (p˃0.05) but significantly higher than those in T1 (103.34 g) (p˂0.05). Feed conversion ratio value which varied from 3.10 – 4.20 were influenced by the treatment (p˂0.05). Rabbits fed diet supplemented with Ficus exasperata oil had a better feed conversion ratio compared to the control (T1). Result obtained indicates that Ficus exasperata oil has the capacity to increase feed intake by improving palatability of diets possibly due to enhanced favour and odor [23]. This outcome confirms the earlier report of [28] who recorded a higher feed intake in finishing pigs fed different levels of Saururus chinensis extracts. This is contrary to the report of [29], disparity in result could be as a result of dosage used, chemical composition of oil, duration of the experiment as well as extraction procedure adopted [17]. Table 4 reveals the apparent digestibility of weaned rabbits fed diet supplemented with Ficus exasperata oil. Dry matter, organic matter, crude protein and ether extract values follow similar pattern and ranged from 73.66 – 86.95 %, 79.43 – 85.67 %, 68.40 – 78.94 % and 48.17 – 60.80 % in that order. In this parameters, rabbits fed T2, T3 and T4 were similar (p˃0.05) but significantly higher than those in T1 (p˂0.05). Conversely, crude fibre value was lower in T1 (59.95 %) than T2 (63.74 %), T3 (65.61 %) and T4 (67.09 %). This outcome suggests that Ficus exasperata oil has the potential to improve villus height to crypt depth and muscular layer of the jejunum and ileum thereby enhancing intestinal absorption capacity [15]. The dietary supplementation of Ficus exasperata oil could also enhance nutrient absorption due to the stimulation of bile, saliva to facilitate the activities of enzymes in the gastro intestinal tract and enhance their health status [17]. This result is in consonance with the reports of [29] who recorded an improvement in apparent digestibility of dry matter, organic matter and crude protein of rabbits fed diet supplemented with rocket seed oil and onion seed oil. Apparent digestibility of weaned rabbits fed diet supplemented with Ficus exasperata oil (Table 4). Nitrogen intake was higher in T4 (15.77 %), intermediate in T2, T3 (12.76 %, 12.85 %) and lower in T1 (9.55 %). Faecal nitrogen and urinary nitrogen values follow similar trend, as faecal nitrogen was higher in T1 (6.90 %) than T2 (4.05 %), T3 (3.96 %) and T4 (2.95 %) (p˂0.05). Urinary nitrogen was lower in T4 (1.05 %), intermediate in T2 (1.28 %), T3 (1.21 %) and lower in T1 (2.59 %) (p˂0.05). This outcome suggests Ficus exasperata oil possess antimicrobial properties which made animals in T2, T3 and T4 have lesser loss of nitrogen needed for growth, this justifies their higher body weight gain. Similarly, nitrogen retention was higher in T4 (56.17 %) than in T3 (50.10 %), T2 (50.02 %) and T1 (43.89 %) (p˂0.05). Rabbits to fed diet supplemented with Ficus exasperata oil retained more nitrogen to produce superior body weight gain. The result obtained in this study is in consonance with the reports of [30,31] when phytogenics was supplemented in the diet of grass cutters.
In conclusion, Ficus exasperata oil is rich in bioactive compounds with therapeutic properties, such as: anti-inflammatory, antioxidant, anti-tumor, immuno-stimulatory, hepato-protective, gastro-protective, cardio-protective, amongst others. Supplementing the oil up to 750 mg/kg DM daily gave a positive result on the growth performance, apparent nutrient digestibility as well as nutrient utilization of rabbits without causing any deleterious effect on the health status.
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I would like to give my testimony in the support I have got by the peer review process and to support the editorial office where they were of asset to support young author like me to be encouraged to publish their work in your respected journal and globalize and share knowledge across the globe. I really give my great gratitude to your journal and the peer review including the editorial office.
I am delighted to publish our manuscript entitled "A Perspective on Cocaine Induced Stroke - Its Mechanisms and Management" in the Journal of Neuroscience and Neurological Surgery. The peer review process, support from the editorial office, and quality of the journal are excellent. The manuscripts published are of high quality and of excellent scientific value. I recommend this journal very much to colleagues.
Dr.Tania Muñoz, My experience as researcher and author of a review article in The Journal Clinical Cardiology and Interventions has been very enriching and stimulating. The editorial team is excellent, performs its work with absolute responsibility and delivery. They are proactive, dynamic and receptive to all proposals. Supporting at all times the vast universe of authors who choose them as an option for publication. The team of review specialists, members of the editorial board, are brilliant professionals, with remarkable performance in medical research and scientific methodology. Together they form a frontline team that consolidates the JCCI as a magnificent option for the publication and review of high-level medical articles and broad collective interest. I am honored to be able to share my review article and open to receive all your comments.
“The peer review process of JPMHC is quick and effective. Authors are benefited by good and professional reviewers with huge experience in the field of psychology and mental health. The support from the editorial office is very professional. People to contact to are friendly and happy to help and assist any query authors might have. Quality of the Journal is scientific and publishes ground-breaking research on mental health that is useful for other professionals in the field”.
Dear editorial department: On behalf of our team, I hereby certify the reliability and superiority of the International Journal of Clinical Case Reports and Reviews in the peer review process, editorial support, and journal quality. Firstly, the peer review process of the International Journal of Clinical Case Reports and Reviews is rigorous, fair, transparent, fast, and of high quality. The editorial department invites experts from relevant fields as anonymous reviewers to review all submitted manuscripts. These experts have rich academic backgrounds and experience, and can accurately evaluate the academic quality, originality, and suitability of manuscripts. The editorial department is committed to ensuring the rigor of the peer review process, while also making every effort to ensure a fast review cycle to meet the needs of authors and the academic community. Secondly, the editorial team of the International Journal of Clinical Case Reports and Reviews is composed of a group of senior scholars and professionals with rich experience and professional knowledge in related fields. The editorial department is committed to assisting authors in improving their manuscripts, ensuring their academic accuracy, clarity, and completeness. Editors actively collaborate with authors, providing useful suggestions and feedback to promote the improvement and development of the manuscript. We believe that the support of the editorial department is one of the key factors in ensuring the quality of the journal. Finally, the International Journal of Clinical Case Reports and Reviews is renowned for its high- quality articles and strict academic standards. The editorial department is committed to publishing innovative and academically valuable research results to promote the development and progress of related fields. The International Journal of Clinical Case Reports and Reviews is reasonably priced and ensures excellent service and quality ratio, allowing authors to obtain high-level academic publishing opportunities in an affordable manner. I hereby solemnly declare that the International Journal of Clinical Case Reports and Reviews has a high level of credibility and superiority in terms of peer review process, editorial support, reasonable fees, and journal quality. Sincerely, Rui Tao.
Clinical Cardiology and Cardiovascular Interventions I testity the covering of the peer review process, support from the editorial office, and quality of the journal.
Clinical Cardiology and Cardiovascular Interventions, we deeply appreciate the interest shown in our work and its publication. It has been a true pleasure to collaborate with you. The peer review process, as well as the support provided by the editorial office, have been exceptional, and the quality of the journal is very high, which was a determining factor in our decision to publish with you.
The peer reviewers process is quick and effective, the supports from editorial office is excellent, the quality of journal is high. I would like to collabroate with Internatioanl journal of Clinical Case Reports and Reviews journal clinically in the future time.
Clinical Cardiology and Cardiovascular Interventions, I would like to express my sincerest gratitude for the trust placed in our team for the publication in your journal. It has been a true pleasure to collaborate with you on this project. I am pleased to inform you that both the peer review process and the attention from the editorial coordination have been excellent. Your team has worked with dedication and professionalism to ensure that your publication meets the highest standards of quality. We are confident that this collaboration will result in mutual success, and we are eager to see the fruits of this shared effort.
Dear Dr. Jessica Magne, Editorial Coordinator 0f Clinical Cardiology and Cardiovascular Interventions, I hope this message finds you well. I want to express my utmost gratitude for your excellent work and for the dedication and speed in the publication process of my article titled "Navigating Innovation: Qualitative Insights on Using Technology for Health Education in Acute Coronary Syndrome Patients." I am very satisfied with the peer review process, the support from the editorial office, and the quality of the journal. I hope we can maintain our scientific relationship in the long term.
Dear Monica Gissare, - Editorial Coordinator of Nutrition and Food Processing. ¨My testimony with you is truly professional, with a positive response regarding the follow-up of the article and its review, you took into account my qualities and the importance of the topic¨.
Dear Dr. Jessica Magne, Editorial Coordinator 0f Clinical Cardiology and Cardiovascular Interventions, The review process for the article “The Handling of Anti-aggregants and Anticoagulants in the Oncologic Heart Patient Submitted to Surgery” was extremely rigorous and detailed. From the initial submission to the final acceptance, the editorial team at the “Journal of Clinical Cardiology and Cardiovascular Interventions” demonstrated a high level of professionalism and dedication. The reviewers provided constructive and detailed feedback, which was essential for improving the quality of our work. Communication was always clear and efficient, ensuring that all our questions were promptly addressed. The quality of the “Journal of Clinical Cardiology and Cardiovascular Interventions” is undeniable. It is a peer-reviewed, open-access publication dedicated exclusively to disseminating high-quality research in the field of clinical cardiology and cardiovascular interventions. The journal's impact factor is currently under evaluation, and it is indexed in reputable databases, which further reinforces its credibility and relevance in the scientific field. I highly recommend this journal to researchers looking for a reputable platform to publish their studies.
Dear Editorial Coordinator of the Journal of Nutrition and Food Processing! "I would like to thank the Journal of Nutrition and Food Processing for including and publishing my article. The peer review process was very quick, movement and precise. The Editorial Board has done an extremely conscientious job with much help, valuable comments and advices. I find the journal very valuable from a professional point of view, thank you very much for allowing me to be part of it and I would like to participate in the future!”
Dealing with The Journal of Neurology and Neurological Surgery was very smooth and comprehensive. The office staff took time to address my needs and the response from editors and the office was prompt and fair. I certainly hope to publish with this journal again.Their professionalism is apparent and more than satisfactory. Susan Weiner
My Testimonial Covering as fellowing: Lin-Show Chin. The peer reviewers process is quick and effective, the supports from editorial office is excellent, the quality of journal is high. I would like to collabroate with Internatioanl journal of Clinical Case Reports and Reviews.
My experience publishing in Psychology and Mental Health Care was exceptional. The peer review process was rigorous and constructive, with reviewers providing valuable insights that helped enhance the quality of our work. The editorial team was highly supportive and responsive, making the submission process smooth and efficient. The journal's commitment to high standards and academic rigor makes it a respected platform for quality research. I am grateful for the opportunity to publish in such a reputable journal.
My experience publishing in International Journal of Clinical Case Reports and Reviews was exceptional. I Come forth to Provide a Testimonial Covering the Peer Review Process and the editorial office for the Professional and Impartial Evaluation of the Manuscript.
I would like to offer my testimony in the support. I have received through the peer review process and support the editorial office where they are to support young authors like me, encourage them to publish their work in your esteemed journals, and globalize and share knowledge globally. I really appreciate your journal, peer review, and editorial office.
Dear Agrippa Hilda- Editorial Coordinator of Journal of Neuroscience and Neurological Surgery, "The peer review process was very quick and of high quality, which can also be seen in the articles in the journal. The collaboration with the editorial office was very good."
I would like to express my sincere gratitude for the support and efficiency provided by the editorial office throughout the publication process of my article, “Delayed Vulvar Metastases from Rectal Carcinoma: A Case Report.” I greatly appreciate the assistance and guidance I received from your team, which made the entire process smooth and efficient. The peer review process was thorough and constructive, contributing to the overall quality of the final article. I am very grateful for the high level of professionalism and commitment shown by the editorial staff, and I look forward to maintaining a long-term collaboration with the International Journal of Clinical Case Reports and Reviews.
To Dear Erin Aust, I would like to express my heartfelt appreciation for the opportunity to have my work published in this esteemed journal. The entire publication process was smooth and well-organized, and I am extremely satisfied with the final result. The Editorial Team demonstrated the utmost professionalism, providing prompt and insightful feedback throughout the review process. Their clear communication and constructive suggestions were invaluable in enhancing my manuscript, and their meticulous attention to detail and dedication to quality are truly commendable. Additionally, the support from the Editorial Office was exceptional. From the initial submission to the final publication, I was guided through every step of the process with great care and professionalism. The team's responsiveness and assistance made the entire experience both easy and stress-free. I am also deeply impressed by the quality and reputation of the journal. It is an honor to have my research featured in such a respected publication, and I am confident that it will make a meaningful contribution to the field.
"I am grateful for the opportunity of contributing to [International Journal of Clinical Case Reports and Reviews] and for the rigorous review process that enhances the quality of research published in your esteemed journal. I sincerely appreciate the time and effort of your team who have dedicatedly helped me in improvising changes and modifying my manuscript. The insightful comments and constructive feedback provided have been invaluable in refining and strengthening my work".
I thank the ‘Journal of Clinical Research and Reports’ for accepting this article for publication. This is a rigorously peer reviewed journal which is on all major global scientific data bases. I note the review process was prompt, thorough and professionally critical. It gave us an insight into a number of important scientific/statistical issues. The review prompted us to review the relevant literature again and look at the limitations of the study. The peer reviewers were open, clear in the instructions and the editorial team was very prompt in their communication. This journal certainly publishes quality research articles. I would recommend the journal for any future publications.
Dear Jessica Magne, with gratitude for the joint work. Fast process of receiving and processing the submitted scientific materials in “Clinical Cardiology and Cardiovascular Interventions”. High level of competence of the editors with clear and correct recommendations and ideas for enriching the article.
We found the peer review process quick and positive in its input. The support from the editorial officer has been very agile, always with the intention of improving the article and taking into account our subsequent corrections.
My article, titled 'No Way Out of the Smartphone Epidemic Without Considering the Insights of Brain Research,' has been republished in the International Journal of Clinical Case Reports and Reviews. The review process was seamless and professional, with the editors being both friendly and supportive. I am deeply grateful for their efforts.
To Dear Erin Aust – Editorial Coordinator of Journal of General Medicine and Clinical Practice! I declare that I am absolutely satisfied with your work carried out with great competence in following the manuscript during the various stages from its receipt, during the revision process to the final acceptance for publication. Thank Prof. Elvira Farina
Dear Jessica, and the super professional team of the ‘Clinical Cardiology and Cardiovascular Interventions’ I am sincerely grateful to the coordinated work of the journal team for the no problem with the submission of my manuscript: “Cardiometabolic Disorders in A Pregnant Woman with Severe Preeclampsia on the Background of Morbid Obesity (Case Report).” The review process by 5 experts was fast, and the comments were professional, which made it more specific and academic, and the process of publication and presentation of the article was excellent. I recommend that my colleagues publish articles in this journal, and I am interested in further scientific cooperation. Sincerely and best wishes, Dr. Oleg Golyanovskiy.
Dear Ashley Rosa, Editorial Coordinator of the journal - Psychology and Mental Health Care. " The process of obtaining publication of my article in the Psychology and Mental Health Journal was positive in all areas. The peer review process resulted in a number of valuable comments, the editorial process was collaborative and timely, and the quality of this journal has been quickly noticed, resulting in alternative journals contacting me to publish with them." Warm regards, Susan Anne Smith, PhD. Australian Breastfeeding Association.
Dear Jessica Magne, Editorial Coordinator, Clinical Cardiology and Cardiovascular Interventions, Auctores Publishing LLC. I appreciate the journal (JCCI) editorial office support, the entire team leads were always ready to help, not only on technical front but also on thorough process. Also, I should thank dear reviewers’ attention to detail and creative approach to teach me and bring new insights by their comments. Surely, more discussions and introduction of other hemodynamic devices would provide better prevention and management of shock states. Your efforts and dedication in presenting educational materials in this journal are commendable. Best wishes from, Farahnaz Fallahian.
Dear Maria Emerson, Editorial Coordinator, International Journal of Clinical Case Reports and Reviews, Auctores Publishing LLC. I am delighted to have published our manuscript, "Acute Colonic Pseudo-Obstruction (ACPO): A rare but serious complication following caesarean section." I want to thank the editorial team, especially Maria Emerson, for their prompt review of the manuscript, quick responses to queries, and overall support. Yours sincerely Dr. Victor Olagundoye.
Dear Ashley Rosa, Editorial Coordinator, International Journal of Clinical Case Reports and Reviews. Many thanks for publishing this manuscript after I lost confidence the editors were most helpful, more than other journals Best wishes from, Susan Anne Smith, PhD. Australian Breastfeeding Association.
Dear Agrippa Hilda, Editorial Coordinator, Journal of Neuroscience and Neurological Surgery. The entire process including article submission, review, revision, and publication was extremely easy. The journal editor was prompt and helpful, and the reviewers contributed to the quality of the paper. Thank you so much! Eric Nussbaum, MD
Dr Hala Al Shaikh This is to acknowledge that the peer review process for the article ’ A Novel Gnrh1 Gene Mutation in Four Omani Male Siblings, Presentation and Management ’ sent to the International Journal of Clinical Case Reports and Reviews was quick and smooth. The editorial office was prompt with easy communication.
Dear Erin Aust, Editorial Coordinator, Journal of General Medicine and Clinical Practice. We are pleased to share our experience with the “Journal of General Medicine and Clinical Practice”, following the successful publication of our article. The peer review process was thorough and constructive, helping to improve the clarity and quality of the manuscript. We are especially thankful to Ms. Erin Aust, the Editorial Coordinator, for her prompt communication and continuous support throughout the process. Her professionalism ensured a smooth and efficient publication experience. The journal upholds high editorial standards, and we highly recommend it to fellow researchers seeking a credible platform for their work. Best wishes By, Dr. Rakhi Mishra.
Dear Jessica Magne, Editorial Coordinator, Clinical Cardiology and Cardiovascular Interventions, Auctores Publishing LLC. The peer review process of the journal of Clinical Cardiology and Cardiovascular Interventions was excellent and fast, as was the support of the editorial office and the quality of the journal. Kind regards Walter F. Riesen Prof. Dr. Dr. h.c. Walter F. Riesen.
Dear Ashley Rosa, Editorial Coordinator, International Journal of Clinical Case Reports and Reviews, Auctores Publishing LLC. Thank you for publishing our article, Exploring Clozapine's Efficacy in Managing Aggression: A Multiple Single-Case Study in Forensic Psychiatry in the international journal of clinical case reports and reviews. We found the peer review process very professional and efficient. The comments were constructive, and the whole process was efficient. On behalf of the co-authors, I would like to thank you for publishing this article. With regards, Dr. Jelle R. Lettinga.
Dear Clarissa Eric, Editorial Coordinator, Journal of Clinical Case Reports and Studies, I would like to express my deep admiration for the exceptional professionalism demonstrated by your journal. I am thoroughly impressed by the speed of the editorial process, the substantive and insightful reviews, and the meticulous preparation of the manuscript for publication. Additionally, I greatly appreciate the courteous and immediate responses from your editorial office to all my inquiries. Best Regards, Dariusz Ziora
Dear Chrystine Mejia, Editorial Coordinator, Journal of Neurodegeneration and Neurorehabilitation, Auctores Publishing LLC, We would like to thank the editorial team for the smooth and high-quality communication leading up to the publication of our article in the Journal of Neurodegeneration and Neurorehabilitation. The reviewers have extensive knowledge in the field, and their relevant questions helped to add value to our publication. Kind regards, Dr. Ravi Shrivastava.
Dear Clarissa Eric, Editorial Coordinator, Journal of Clinical Case Reports and Studies, Auctores Publishing LLC, USA Office: +1-(302)-520-2644. I would like to express my sincere appreciation for the efficient and professional handling of my case report by the ‘Journal of Clinical Case Reports and Studies’. The peer review process was not only fast but also highly constructive—the reviewers’ comments were clear, relevant, and greatly helped me improve the quality and clarity of my manuscript. I also received excellent support from the editorial office throughout the process. Communication was smooth and timely, and I felt well guided at every stage, from submission to publication. The overall quality and rigor of the journal are truly commendable. I am pleased to have published my work with Journal of Clinical Case Reports and Studies, and I look forward to future opportunities for collaboration. Sincerely, Aline Tollet, UCLouvain.
Dear Ms. Mayra Duenas, Editorial Coordinator, International Journal of Clinical Case Reports and Reviews. “The International Journal of Clinical Case Reports and Reviews represented the “ideal house” to share with the research community a first experience with the use of the Simeox device for speech rehabilitation. High scientific reputation and attractive website communication were first determinants for the selection of this Journal, and the following submission process exceeded expectations: fast but highly professional peer review, great support by the editorial office, elegant graphic layout. Exactly what a dynamic research team - also composed by allied professionals - needs!" From, Chiara Beccaluva, PT - Italy.
Dear Maria Emerson, Editorial Coordinator, we have deeply appreciated the professionalism demonstrated by the International Journal of Clinical Case Reports and Reviews. The reviewers have extensive knowledge of our field and have been very efficient and fast in supporting the process. I am really looking forward to further collaboration. Thanks. Best regards, Dr. Claudio Ligresti
Dear Chrystine Mejia, Editorial Coordinator, Journal of Neurodegeneration and Neurorehabilitation. “The peer review process was efficient and constructive, and the editorial office provided excellent communication and support throughout. The journal ensures scientific rigor and high editorial standards, while also offering a smooth and timely publication process. We sincerely appreciate the work of the editorial team in facilitating the dissemination of innovative approaches such as the Bonori Method.” Best regards, Dr. Matteo Bonori.
I recommend without hesitation submitting relevant papers on medical decision making to the International Journal of Clinical Case Reports and Reviews. I am very grateful to the editorial staff. Maria Emerson was a pleasure to communicate with. The time from submission to publication was an extremely short 3 weeks. The editorial staff submitted the paper to three reviewers. Two of the reviewers commented positively on the value of publishing the paper. The editorial staff quickly recognized the third reviewer’s comments as an unjust attempt to reject the paper. I revised the paper as recommended by the first two reviewers.
Dear Maria Emerson, Editorial Coordinator, Journal of Clinical Research and Reports. Thank you for publishing our case report: "Clinical Case of Effective Fetal Stem Cells Treatment in a Patient with Autism Spectrum Disorder" within the "Journal of Clinical Research and Reports" being submitted by the team of EmCell doctors from Kyiv, Ukraine. We much appreciate a professional and transparent peer-review process from Auctores. All research Doctors are so grateful to your Editorial Office and Auctores Publishing support! I amiably wish our article publication maintained a top quality of your International Scientific Journal. My best wishes for a prosperity of the Journal of Clinical Research and Reports. Hope our scientific relationship and cooperation will remain long lasting. Thank you very much indeed. Kind regards, Dr. Andriy Sinelnyk Cell Therapy Center EmCell
Dear Editorial Team, Clinical Cardiology and Cardiovascular Interventions. It was truly a rewarding experience to work with the journal “Clinical Cardiology and Cardiovascular Interventions”. The peer review process was insightful and encouraging, helping us refine our work to a higher standard. The editorial office offered exceptional support with prompt and thoughtful communication. I highly value the journal’s role in promoting scientific advancement and am honored to be part of it. Best regards, Meng-Jou Lee, MD, Department of Anesthesiology, National Taiwan University Hospital.
Dear Editorial Team, Journal-Clinical Cardiology and Cardiovascular Interventions, “Publishing my article with Clinical Cardiology and Cardiovascular Interventions has been a highly positive experience. The peer-review process was rigorous yet supportive, offering valuable feedback that strengthened my work. The editorial team demonstrated exceptional professionalism, prompt communication, and a genuine commitment to maintaining the highest scientific standards. I am very pleased with the publication quality and proud to be associated with such a reputable journal.” Warm regards, Dr. Mahmoud Kamal Moustafa Ahmed
Dear Maria Emerson, Editorial Coordinator of ‘International Journal of Clinical Case Reports and Reviews’, I appreciate the opportunity to publish my article with your journal. The editorial office provided clear communication during the submission and review process, and I found the overall experience professional and constructive. Best regards, Elena Salvatore.
Dear Mayra Duenas, Editorial Coordinator of ‘International Journal of Clinical Case Reports and Reviews Herewith I confirm an optimal peer review process and a great support of the editorial office of the present journal
Dear Editorial Team, Clinical Cardiology and Cardiovascular Interventions. I am really grateful for the peers review; their feedback gave me the opportunity to reflect on the message and impact of my work and to ameliorate the article. The editors did a great job in addition by encouraging me to continue with the process of publishing.
Dear Cecilia Lilly, Editorial Coordinator, Endocrinology and Disorders, Thank you so much for your quick response regarding reviewing and all process till publishing our manuscript entitled: Prevalence of Pre-Diabetes and its Associated Risk Factors Among Nile College Students, Sudan. Best regards, Dr Mamoun Magzoub.
International Journal of Clinical Case Reports and Reviews is a high quality journal that has a clear and concise submission process. The peer review process was comprehensive and constructive. Support from the editorial office was excellent, since the administrative staff were responsive. The journal provides a fast and timely publication timeline.
Dear Maria Emerson, Editorial Coordinator of International Journal of Clinical Case Reports and Reviews, What distinguishes International Journal of Clinical Case Report and Review is not only the scientific rigor of its publications, but the intellectual climate in which research is evaluated. The submission process is refreshingly free of unnecessary formal barriers and bureaucratic rituals that often complicate academic publishing without adding real value. The peer-review system is demanding yet constructive, guided by genuine scientific dialogue rather than hierarchical or authoritarian attitudes. Reviewers act as collaborators in improving the manuscript, not as gatekeepers imposing arbitrary standards. This journal offers a rare balance: high methodological standards combined with a respectful, transparent, and supportive editorial approach. In an era where publishing can feel more burdensome than research itself, this platform restores the original purpose of peer review — to refine ideas, not to obstruct them Prof. Perlat Kapisyzi, FCCP PULMONOLOGIST AND THORACIC IMAGING.
Dear Grace Pierce, International Journal of Clinical Case Reports and Reviews I appreciate the opportunity to review for Auctore Journal, as the overall editorial process was smooth, transparent and professionally managed. This journal maintains high scientific standards and ensures timely communications with authors, which is truly commendable. I would like to express my special thanks to editor Grace Pierce for his constant guidance, promt responses, and supportive coordination throughout the review process. I am also greatful to Eleanor Bailey from the finance department for her clear communication and efficient handling of all administrative matters. Overall, my experience with Auctore Journal has been highly positive and rewarding. Best regards, Sabita sinha
Dear Mayra Duenas, Editorial Coordinator of the journal IJCCR, I write here a little on my experience as an author submitting to the International Journal of Clinical Case Reports and Reviews (IJCCR). This was my first submission to IJCCR and my manuscript was inherently an outsider’s effort. It attempted to broadly identify and then make some sense of life’s under-appreciated mysteries. I initially had responded to a request for possible submissions. I then contacted IJCCR with a tentative topic for a manuscript. They quickly got back with an approval for the submission, but with a particular requirement that it be medically relevant. I then put together a manuscript and submitted it. After the usual back-and-forth over forms and formality, the manuscript was sent off for reviews. Within 2 weeks I got back 4 reviews which were both helpful and also surprising. Surprising in that the topic was somewhat foreign to medical literature. My subsequent updates in response to the reviewer comments went smoothly and in short order I had a series of proofs to evaluate. All in all, the whole publication process seemed outstanding. It was both helpful in terms of the paper’s content and also in terms of its efficient and friendly communications. Thank you all very much. Sincerely, Ted Christopher, Rochester, NY.
