AUCTORES
Globalize your Research
Research Article | DOI: https://doi.org/10.31579/2693-7247/105
1 Debre Markos University, College of Natural and Computational Sciences, Department of Chemistry, Ethiopia.
2 Injibara University, College of Natural and Computational Sciences, Department of Chemistry, Ethiopia.
*Corresponding Author: M. A Omoirri, Department of Pharmacology and Toxicology, Faculty of Pharmaceutical Sciences, Federal University of Oye-Ekiti, Ekiti State, Nigeria.
Citation: Omoirri Moses Aziakpono, Augustine Wisdom Ehimen, Frank Okezie Eze, Maduka Kate Ifeanyi and Osagie Mike Odigie, (2022), Evaluation of the Anti-inflammatory Activity of the Leaf Extract and Fractions of ipomoea Quamoclit in Albino Wistar Rats. J. Pharmaceutics and Pharmacology Research, 5(9); DOI:10.31579/2693-7247/105
Copyright: © 2022 M. A Omoirri, This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Received: 19 September 2022 | Accepted: 21 October 2022 | Published: 17 November 2022
Keywords: ipomoea quamoclit; inflammation; phytochemicals; fractions
Background: Medicinal plants have been shown to contain intrinsic active ingredients that can be used for curative purpose for some diseases. Ipomoea quamoclit is one of such ethnomedicinal plants of reported health benefits.
Aim of study: This study was aimed at evaluating the anti-inflammatory activity of ethanol extract and fractions of the leaves of Ipomoea quamoclit Mill.
Objectives of the study: These include: crude extraction and fractionation of Ipomoea quamoclit leaves; evaluation of the anti-inflammatory and anti-arthritic properties of leaf extract and fractions of Ipomoea quamoclit. Materials and Methods: Ipomoea quamoclit leaves were harvested and extracted with 80% ethanol by cold maceration for 48 hours and the crude extract was fractionated to obtain n-hexane, n-butanol and ethyl acetate fraction, respectively. The crude extract and its fractions were screened to unveil the resident phytochemical constituents. The ethanol extract was tested for acute toxicity (LD50). The systemic acute inflammation of the crude extract and fractions of Ipomoea quamoclit was studied using the water (fluid) displacement method after inducing inflammation with egg albumin, using rats. The effect of the crude extract and fractions of Ipomoea quamoclit on arthritis was studied using the formaldehyde-induced arthritis model in rats.
Results and Conclusions: The results of the qualitative phytochemical evaluation of the plant confirmed the presence of these phytoconstituents such as alkaloids, flavonoids, saponins, tannins, steroids, terpenoids, glycosides, carbohydrates, proteins, and coumarins. There were no deaths recorded for acute toxicity study for both phases of the test. In the acute anti-inflammation assay, significant (P<0.05) anti-inflammatory activities were observed from the 5th hour for the ethyl acetate fraction (150 and 400 mg/kg) and the butanol fraction (400 mg/kg). The anti-arthritic activity of the crude extract (150 mg/kg), ethyl acetate fraction (150 and 400 mg/kg), n-hexane fraction (150 mg/kg) and butanol fraction (150 and 400 mg/kg) was obvious from the second day after administration. By the 10th day the crude extract (50, 150, and 400 mg/kg) and ethyl acetate fraction (150 and 400 mg/kg) brought about a significant reduction of chronic inflammation (arthritis) in the test animals to normal basal levels. This study thus provides scientific information and validates the ethnobotanical use of Ipomoea quamoclit leaves in management of diseases and inflammation inclusive. The findings of this study also reveal that potentially Ipomoea quamoclit could be a source of pharmacologically active compounds of pharmaceutical importance.
Globally, plants which constitute a major component of foodstuffs are sources of therapeutic agents for cure and prevention of diseases. At present more than 35,000 plants species are used for medicinal purposes globally (Yirga et al., 2011).
Plants and plant products have long been used in traditional medicine to treat infectious diseases. The use of medicinal plants remains significant as therapeutic remedies and still plays an important role in primary health care in developing countries such as Nigeria (Onyebule et al., 2014).
For many years’ people depended solely on leaves, flowers and barks of plants for medicine. However, in recent times synthetic drugs which are either replicas or modifications of chemicals identified in plant are being used. (Conway, 1973).
Investigations into the chemical and biological activities of plants during the past two decades have yielded compounds for the development of modern synthetic organic chemistry as a major route for the discovery of novel and more effective therapeutic agents (Roja and Rao, 2002).
Several medicinal plants products are used in Africa for the management of pain and inflammatory conditions with traditional affirmation of their efficacy and potency (Umar et al., 2013). Phytochemicals with various bioactivities including antioxidant, anti-inflammatory and chemotherapeutic properties are extensively extracted from many plant species (Khalita et al., 2013).
In conventional Western medicine, 50-60% of pharmaceutical commodities contain natural products or their derivatives (Cameron et al.,2005). There is a growing interest in correlating phytochemical constituents of plants with their pharmacological activity (Vukovic et al.,2007). Flavonoids have been reported to possess antibacterial activity just as purified alkaloids and their synthetic products are used for various biological effects including antispasmodics, analgesics and antibacterial remedies (Evans, 2002).
Ipomoea quamoclit L. is a less studied medicinal plant, which is an annual, herbaceous; twining vine belongs to the family Convolvulaceae. It is commonly known as cypress vine, star glory, cardinal vine, cardinal creeper, Indian pink, hummingbird vine and cupid’s flower (Chetty et al. 2008, Haque and Ghosh 2013). Ipomoea quamoclit is one of the most commonly seen plant throughout the tropics in and around of the living area from northern South America to Mexico. In India, it is called mayil manikkam, akasamulla, kunjalata, tarulata, kamalata, getphul etc. Plant annual or perennial herb, twining vine which grows upto a height of 1–3 m. Leaves are long (2-9 cm), pinnately lobed, each side of the leaf containing 9-19 lobes. The flowers are long (3-4 cm) with a diameter of 2 cm, five points trumpet-shaped, and can be red, pink or white. It flowers in summer and fall (Lowell and Lucansky 1990).
Ipomoea quamoclit is used as folk medicine around the world for various illnesses (Rajendran et al. 2007, Sajem et al. 2008). The plant is considered cooling and purgative; used in chest pain. Pounded leaves are used as remedy for bleeding piles and carbuncles (Pullaiah et al. 1997, Yusuf et al. 2009). Leaves are also used as poultices for bleeding haemorrhoids (Kumar & Akhtar 2013, Sorathia 2014). Plant found its significant use in Siddha medicine where the decoction of leaves and stems are used to treat fever, diabetes and in Thailand, it is used for snake bites and bloody cough (Khare 2007, Hasan et al. 2009).
Aim of study
This study evaluates the anti-inflammatory properties of ethanol extract and fractions of the leaves of Ipomoea quamoclit. Specifically, study;
Test Animals
Healthy adult Albino Wistar rats of either sex weighing between 200 - 250 g were used for the experiments. The animals were obtained from the animal house of Department of Pharmacology and Toxicology, Faculty of Pharmaceutical Sciences, Nnamdi Azikiwe University, Awka, Anambra State, Nigeria. They were maintained under standard environmental conditions with free access to standard diet and water ad libitum. All animal experiments were conducted in compliance with NIH Guide for Care and Use of Laboratory Animals (Pub. No. 85-23 Revised 1985).
Sample Collection, Identification and Preparation
Fresh leaves of Avocado Ipomoea quamoclit were collected from Awka in the month of October, 2018. The identity of the plant was authenticated by a plant expert at the Department of Pharmacognosy and Traditional Medicine, Nnamdi Azikiwe University, Awka, Anambra State, Nigeria and a voucher specimen deposited (N.a.u.h.n-183a). The leaves were air dried, and the dried leaves were then pulverized to fine powder.
Extraction of Persea americana
i. Crude Extraction
Extraction of the plant leaves was carried out as described by Onyegbule et al. (2014). The pulverized leaves were cold-macerated in ethanol 48 h and filtered. The filtrates were concentrated in a rotary evaporator at 40oC.
ii. Fractionation
Fractionation of the crude ethanol leaf extracts of the plant was carried out as described by Onyegbule et al. (2014). The extracts were fractionated using the liquid-liquid fractionation method with ethyl acetate, n-hexane, and n-butanol. The filtrates obtained were concentrated in a rotary evaporator at 40oC.
Determination of Percentage Yield
The percentage yield for the leaf and seed extract and fractions was calculated and recorded.
Percentage (%) yield= Weight (g) of concentrated leaf or stem extract X 100
Weight (g) of powder leaves or stem 1
Qualitative Phytochemical Analysis
Screening for presence of secondary metabolites (flavonoids, alkaloids, reducing sugars, tannins and saponins, etc. in Ipomoea quamoclit leaves was performed following standard phytochemical tests. The test was carried out according to the procedures outlined by Trease and Evans (2002). Ten percent (10%) preparation of the extract in distilled water was considered as the test samples. Distilled water was used as a negative control throughout the phytochemical tests.
Determination of LD50
The acute toxicity (LD50) of the extract was determined in order to define the range of the lethal dose and the safe range for the extract. The test was carried out in two phases as described by Lorke (1983) using a total of 13 rat of weight 200-250g. In the first phase, the animals were divided into 3 groups of 3 rats each, and the extract was administered at three dose level (10, 100 and 1000 mg/kg) body weight. The animals were then monitored for 24 hours. Based on the results obtained from this first phase, the remaining animals were then grouped into 4 groups of 1 animal each for second phase of the test. In the second phase, 4 dose range were also used 800, 1600, 2900 and 5000 mg/kg body weight. Each dose was administered to one specific group only and the animals were examined again for another 24 hours.
Study Design
Acute inflammatory test
A total of 55 rats were used. They were grouped into 11 groups of five rats per group as follows;
Group 1 received 10ml/kg of distilled water
Group 2 received 50mg/kg of diclofenac
Group 3 received 50mg/kg of crude extract
Group 4 received 150mg/kg of crude extract
Group 5 received 400mg/kg of crude extract
Group 6 received 150mg/kg of ethyl acetate fraction
Group 7 received 400mg/kg of ethyl acetate fraction
Group 8 received 150mg/kg of n-hexane fraction
Group 9 received 400mg/kg of n-hexane fraction
Group 10 received 150mg/kg of butanol fraction
Group 11 received 400mg/kg of butanol fraction
One (1) hour post-treatment, the animals received sub-plantar injection of 0.1 ml of fresh egg albumin in the right hind paw. The paw size was measured before (basal) and at 1, 2, 3, 4 and 5 hour(s) after egg albumin injection.
Chronic inflammation (arthritis) study
The effect of the crude extract and fractions of Ipomoea quamoclit on arthritis was studied using the formaldehyde-induced arthritis model in rats as described by (Udegbunam et al., 2014). A total of 55 rats were used. They were grouped into 11 groups of 5 rats per group.
Group 1 received 10ml/kg of distilled water
Group 2 received 50mg/kg of diclofenac
Group 3 received 50mg/kg of crude extract
Group 4 received 150mg/kg of crude extract
Group 5 received 400mg/kg of crude extract
Group 6 received 150mg/kg of ethyl acetate fraction
Group 7 received 400mg/kg of ethyl acetate fraction
Group 8 received 150mg/kg of n-hexane fraction
Group 9 received 400mg/kg of n-hexane fraction
Group 10 received 150mg/kg of butanol fraction
Group 11 received 400mg/kg of butanol fraction
One (1) hour after administration, the animals received sub-plantar injection of 0.1ml of 2.5% (v/v) formaldehyde in the right hind paw on day zero. The paw volume was measured before (basal) and once every day for 10 days. On day 3, the paw was re-inflamed with formaldehyde. The extract was administered once daily for the 10 days. The volume of water displaced by the inflamed paw was used as measure of edema. Increase in paw volume is quantified as inflammation. Percentage inhibition of edema was calculated using the formula
100 * [1-(a-x/b-y)]
Where a = mean paw volume of treated rat at various times after formaldehyde injection
x = mean paw volume of treated rats before formaldehyde injection
b = mean paw volume of control rats at various times after formaldehyde injection
y = mean paw volume of control rats before formaldehyde injection.
Statistical Analysis
Data obtained from the study were analyzed using Statistical Package for Social Sciences (SPSS-21). Results was expressed as mean ± SEM. Raw data were subjected to one way analyses of variances (ANOVA) followed by post hoc Tukey’s test. P< 0>
Determination of percentage yield
The crude ethanol extract of Ipomoea quamoclit derived from the cold maceration of the powdered dried plant material was fractionated into ethyl acetate, n-hexane, and butanol fractions. The yield from the extraction and fractionation of Ipomoea quamoclit is shown in Table 1.
Table 1: Percentage Yield of the crude extract and fractions of Ipomoea quamoclit Leaves
Acute toxicity (LD50) of Ipomoea quamoclit
The LD50 of the extract was above 5000mg/kg as no death was recorded at the highest dose after 24hours monitoring. There was no sign of weakness, anorexia or restlessness observed within 24 hours monitoring.
Qualitative phytochemical analysis of Ipomoea quamoclit leaves
Qualitative phytochemical analysis of Ipomoea quamoclit leaves was conducted using standard methods. It can be observed from the table, that alkaloids, flavonoids, saponins, tannins, steroids, terpenoids, glycosides, carbohydrates, proteins, and coumarins were detected in the plant leaves; while carotenoids and anthraquinones were absent (Table 2).
(-) => Not Present, (+) => Present in small concentration, (++) => Present in moderately high concentration, (+++) => Present in high concentration
Table 2: Phytochemical Analysis
Acute-inflammation
The systemic acute inflammation of the crude extract and fractions of Ipomoea quamoclit was studied using the water (fluid) displacement method.
The plant extract and fractions showed varying levels of anti-inflammatory activity and results are presented in Figure 1. Significant (P<0>
Figure 1: Effect of crude extract of Ipomoea quamoclit Leaves on fresh egg albumin induced paw oedema
Figure 2: Effect of Formaldehyde-induced paw oedema of fractions extract of Ipomoea quamoclit Leaves
Chronic inflammation (arthritis) assay
The effect of the crude extract and fractions of Ipomoea quamoclit on arthritis was studied using the Formaldehyde-induced arthritis model in rats.
The plant extract and fractions showed varying levels of anti-arthritic activity and results of the anti-arthritis assay are presented in Figure 2.
Anti-arthritic activity of the crude extract (150 mg/kg), ethyl acetate fraction (150 and 400 mg/kg), n-hexane fraction (150 mg/kg) and butanol fraction (150 and 400 mg/kg) was obvious from the second day after administration.
By the 10th day the crude extract (50, 150, and 400 mg/kg) and ethyl acetate fraction (150 and 400 mg/kg), just like the standard drug- diclofenac (50 mg/kg), brought about a significant (P<0>
Table 3: AUC of ac inflammation of crude extract of P. americana leaves
Table 4: AUC of chronic inflammation of fraction of P. americana leaves
Inflammation is a protective response purposely to get rid of organism that causes injury (e.g. microbes and toxins) and the consequence of the injury (e.g. the damage of cell and tissue). Without inflammation, infection will continue developing, wounds will not heal, and the injured organ will continue suppurating. In the process of inflammation, there is a process in which free radical compounds are generated (Ardhie, 2011). Free radicals can cause tissue decay that triggers biosynthesis of arachidonic acid into prostaglandin as the mediator of inflammation (Sanchez et al., 2015). Excessive inflammatory responses or severe tissue decay should be handled immediately, thus inflammatory reaction should be treated to reduce signs and symptoms (Meliala and Pinzon, 2007). General symptoms of inflammation such as swelling, heat, pain, redness, and the loss of function can cause discomfort for the patient so that it needs to be handled (Kristanti et al., 2017). In that case, anti-inflammatory and analgesic compounds are needed.
Ipomoea quamoclit is used in traditional medicine practice with validated claims. The leaves of Ipomoea quamoclit have been used as anticancer (Renuka and Ravishankar 2014), antioxidant (Hasan et al., 2009), antimicrobial (Moin et al., 2012), antidiabetic (Reddy et al., 2015) and Insecticidal Activity (Prodhan et al., 2012)
In this study, the crude extract and fractions of Ipomoea quamoclit showed varying levels of anti-inflammatory activity. This finding are similar to that of (Nworu and Akah 2015), using the plant extract of cyclotide-rich in a similar mode.
These findings are consistent with those of previous studies (Adeyemi et al, 2003) despite differences in experimental design, extraction protocol, solvent, solvent strengths, experimental animals (species, weight, age, gender), animal models, reference standards, sources of plants and time of collection. The anti-inflammatory activities of Ipomoea quamoclit may be attributed to the presence of secondary metabolites and pharmacologically active compounds such as saponins, tannins, flavonoids, fatty acids, steroids, alkaloids. Available reports indicate the biological activities of saponins from medicinal plants are linked to their amphiphilic nature, exhibiting the activities via their capabilities to intercalate into the plasma membrane culminating in changes in membrane fluidity that in turn affect membrane function, thus bringing about cellular response (Hassan et al., 2015). The mechanism for the anti-inflammatory activities of Ipomoea quamoclit may be due to their inhibition of arachidonic acid metabolism, pro-inflammatory cytokines, inducible nitric oxide, transcription activation (NF-eB) (Nworu and Akah, 2015). The varying anti-inflammatory activities may be due on type of formulation (crude extract or fraction), polarity (polar or nonpolar), dose, phytochemical content, binding affinities, physicochemical properties and bioavailability of formulations (Antia et al, 2015). The study found biphasic increases in anti-arthritic activities associated with the crude extract and some fractions but the biphasic increases and decreases differed based on time, formulation, treatment, (extract or fraction) and dose. The present study found that the anti-arthritic activity was a multiphasic phenomenin fluctuating, waxing and waning over time. The anti-arthritic activities were characterized by a first increase followed by first slump, then second increase and second slump or decrease in ant-inflammatory activity. The study confirmed the characteristic Day 2-5 increase in anti-inflammatory activities found in previous studies (Adeyemi et al., 2012). In contrast this present study reported a second slump that occurred on Days 2 and 6-7 not reported by previous studies due to the limited observation period (Kamau et al, 2016) (4 hours). The first increase may be attributed to the release of histamine, serotonin, mast cells, basophils, neutrophils and cyclooxygenase and arachidonic acid metabolism while the first slump may be due to the metabolism of inflammatory cells and mediators. Also, the second increase in anti-inflammatory activities may be due to the secretion of pro-inflammatory mediators such as prostaglandin PGE2, cytokines, interleukins and interferons while the second slump (percentage inhibition) may be due to the binding of inflammatory mediators to their respective receptors, redistribution to systemic circulation, metabolism of pro-inflammatory mediators or down-regulation of bound receptors.
Pharmacologically active substances (phytochemical composition, concentration of bioactive agents with anti-arthritic and anti-inflammatory properties) may have to be released from the formulation (bioavailability) which possess the ability to penetrate the cell membrane (physicochemical properties, concentration, dose, lipid: water partition coefficient) in sufficient concentration over time to achieve and sustain a maximum effect. The time to reach the maximum anti-arthritic activity may depend on the fraction, phytochemical composition, AUC, dose, concentration, physicochemical properties, bio availabilities of pharmacological active and anti-arthritic compounds.
In this study, the ethanol leaf extract and fractions of Ipomoea quamoclit was evaluated for anti-inflammatory. The crude extract and fractions of the Ipomoea quamoclit leaves displayed various degrees of anti-inflammatory, which confirms and validates the ethnobotanical use of the plant leaves in disease management; and also reveal the potentials of Ipomoea quamoclit as a source of pharmacologically active compounds of pharmaceutical importance.
Clearly Auctoresonline and particularly Psychology and Mental Health Care Journal is dedicated to improving health care services for individuals and populations. The editorial boards' ability to efficiently recognize and share the global importance of health literacy with a variety of stakeholders. Auctoresonline publishing platform can be used to facilitate of optimal client-based services and should be added to health care professionals' repertoire of evidence-based health care resources.
Journal of Clinical Cardiology and Cardiovascular Intervention The submission and review process was adequate. However I think that the publication total value should have been enlightened in early fases. Thank you for all.
Journal of Women Health Care and Issues By the present mail, I want to say thank to you and tour colleagues for facilitating my published article. Specially thank you for the peer review process, support from the editorial office. I appreciate positively the quality of your journal.
Journal of Clinical Research and Reports I would be very delighted to submit my testimonial regarding the reviewer board and the editorial office. The reviewer board were accurate and helpful regarding any modifications for my manuscript. And the editorial office were very helpful and supportive in contacting and monitoring with any update and offering help. It was my pleasure to contribute with your promising Journal and I am looking forward for more collaboration.
We would like to thank the Journal of Thoracic Disease and Cardiothoracic Surgery because of the services they provided us for our articles. The peer-review process was done in a very excellent time manner, and the opinions of the reviewers helped us to improve our manuscript further. The editorial office had an outstanding correspondence with us and guided us in many ways. During a hard time of the pandemic that is affecting every one of us tremendously, the editorial office helped us make everything easier for publishing scientific work. Hope for a more scientific relationship with your Journal.
The peer-review process which consisted high quality queries on the paper. I did answer six reviewers’ questions and comments before the paper was accepted. The support from the editorial office is excellent.
Journal of Neuroscience and Neurological Surgery. I had the experience of publishing a research article recently. The whole process was simple from submission to publication. The reviewers made specific and valuable recommendations and corrections that improved the quality of my publication. I strongly recommend this Journal.
Dr. Katarzyna Byczkowska My testimonial covering: "The peer review process is quick and effective. The support from the editorial office is very professional and friendly. Quality of the Clinical Cardiology and Cardiovascular Interventions is scientific and publishes ground-breaking research on cardiology that is useful for other professionals in the field.
Thank you most sincerely, with regard to the support you have given in relation to the reviewing process and the processing of my article entitled "Large Cell Neuroendocrine Carcinoma of The Prostate Gland: A Review and Update" for publication in your esteemed Journal, Journal of Cancer Research and Cellular Therapeutics". The editorial team has been very supportive.
Testimony of Journal of Clinical Otorhinolaryngology: work with your Reviews has been a educational and constructive experience. The editorial office were very helpful and supportive. It was a pleasure to contribute to your Journal.
Dr. Bernard Terkimbi Utoo, I am happy to publish my scientific work in Journal of Women Health Care and Issues (JWHCI). The manuscript submission was seamless and peer review process was top notch. I was amazed that 4 reviewers worked on the manuscript which made it a highly technical, standard and excellent quality paper. I appreciate the format and consideration for the APC as well as the speed of publication. It is my pleasure to continue with this scientific relationship with the esteem JWHCI.
This is an acknowledgment for peer reviewers, editorial board of Journal of Clinical Research and Reports. They show a lot of consideration for us as publishers for our research article “Evaluation of the different factors associated with side effects of COVID-19 vaccination on medical students, Mutah university, Al-Karak, Jordan”, in a very professional and easy way. This journal is one of outstanding medical journal.
Dear Hao Jiang, to Journal of Nutrition and Food Processing We greatly appreciate the efficient, professional and rapid processing of our paper by your team. If there is anything else we should do, please do not hesitate to let us know. On behalf of my co-authors, we would like to express our great appreciation to editor and reviewers.
As an author who has recently published in the journal "Brain and Neurological Disorders". I am delighted to provide a testimonial on the peer review process, editorial office support, and the overall quality of the journal. The peer review process at Brain and Neurological Disorders is rigorous and meticulous, ensuring that only high-quality, evidence-based research is published. The reviewers are experts in their fields, and their comments and suggestions were constructive and helped improve the quality of my manuscript. The review process was timely and efficient, with clear communication from the editorial office at each stage. The support from the editorial office was exceptional throughout the entire process. The editorial staff was responsive, professional, and always willing to help. They provided valuable guidance on formatting, structure, and ethical considerations, making the submission process seamless. Moreover, they kept me informed about the status of my manuscript and provided timely updates, which made the process less stressful. The journal Brain and Neurological Disorders is of the highest quality, with a strong focus on publishing cutting-edge research in the field of neurology. The articles published in this journal are well-researched, rigorously peer-reviewed, and written by experts in the field. The journal maintains high standards, ensuring that readers are provided with the most up-to-date and reliable information on brain and neurological disorders. In conclusion, I had a wonderful experience publishing in Brain and Neurological Disorders. The peer review process was thorough, the editorial office provided exceptional support, and the journal's quality is second to none. I would highly recommend this journal to any researcher working in the field of neurology and brain disorders.
Dear Agrippa Hilda, Journal of Neuroscience and Neurological Surgery, Editorial Coordinator, I trust this message finds you well. I want to extend my appreciation for considering my article for publication in your esteemed journal. I am pleased to provide a testimonial regarding the peer review process and the support received from your editorial office. The peer review process for my paper was carried out in a highly professional and thorough manner. The feedback and comments provided by the authors were constructive and very useful in improving the quality of the manuscript. This rigorous assessment process undoubtedly contributes to the high standards maintained by your journal.
International Journal of Clinical Case Reports and Reviews. I strongly recommend to consider submitting your work to this high-quality journal. The support and availability of the Editorial staff is outstanding and the review process was both efficient and rigorous.
Thank you very much for publishing my Research Article titled “Comparing Treatment Outcome Of Allergic Rhinitis Patients After Using Fluticasone Nasal Spray And Nasal Douching" in the Journal of Clinical Otorhinolaryngology. As Medical Professionals we are immensely benefited from study of various informative Articles and Papers published in this high quality Journal. I look forward to enriching my knowledge by regular study of the Journal and contribute my future work in the field of ENT through the Journal for use by the medical fraternity. The support from the Editorial office was excellent and very prompt. I also welcome the comments received from the readers of my Research Article.
Dear Erica Kelsey, Editorial Coordinator of Cancer Research and Cellular Therapeutics Our team is very satisfied with the processing of our paper by your journal. That was fast, efficient, rigorous, but without unnecessary complications. We appreciated the very short time between the submission of the paper and its publication on line on your site.
I am very glad to say that the peer review process is very successful and fast and support from the Editorial Office. Therefore, I would like to continue our scientific relationship for a long time. And I especially thank you for your kindly attention towards my article. Have a good day!
"We recently published an article entitled “Influence of beta-Cyclodextrins upon the Degradation of Carbofuran Derivatives under Alkaline Conditions" in the Journal of “Pesticides and Biofertilizers” to show that the cyclodextrins protect the carbamates increasing their half-life time in the presence of basic conditions This will be very helpful to understand carbofuran behaviour in the analytical, agro-environmental and food areas. We greatly appreciated the interaction with the editor and the editorial team; we were particularly well accompanied during the course of the revision process, since all various steps towards publication were short and without delay".
I would like to express my gratitude towards you process of article review and submission. I found this to be very fair and expedient. Your follow up has been excellent. I have many publications in national and international journal and your process has been one of the best so far. Keep up the great work.
We are grateful for this opportunity to provide a glowing recommendation to the Journal of Psychiatry and Psychotherapy. We found that the editorial team were very supportive, helpful, kept us abreast of timelines and over all very professional in nature. The peer review process was rigorous, efficient and constructive that really enhanced our article submission. The experience with this journal remains one of our best ever and we look forward to providing future submissions in the near future.
I am very pleased to serve as EBM of the journal, I hope many years of my experience in stem cells can help the journal from one way or another. As we know, stem cells hold great potential for regenerative medicine, which are mostly used to promote the repair response of diseased, dysfunctional or injured tissue using stem cells or their derivatives. I think Stem Cell Research and Therapeutics International is a great platform to publish and share the understanding towards the biology and translational or clinical application of stem cells.
I would like to give my testimony in the support I have got by the peer review process and to support the editorial office where they were of asset to support young author like me to be encouraged to publish their work in your respected journal and globalize and share knowledge across the globe. I really give my great gratitude to your journal and the peer review including the editorial office.
I am delighted to publish our manuscript entitled "A Perspective on Cocaine Induced Stroke - Its Mechanisms and Management" in the Journal of Neuroscience and Neurological Surgery. The peer review process, support from the editorial office, and quality of the journal are excellent. The manuscripts published are of high quality and of excellent scientific value. I recommend this journal very much to colleagues.
Dr.Tania Muñoz, My experience as researcher and author of a review article in The Journal Clinical Cardiology and Interventions has been very enriching and stimulating. The editorial team is excellent, performs its work with absolute responsibility and delivery. They are proactive, dynamic and receptive to all proposals. Supporting at all times the vast universe of authors who choose them as an option for publication. The team of review specialists, members of the editorial board, are brilliant professionals, with remarkable performance in medical research and scientific methodology. Together they form a frontline team that consolidates the JCCI as a magnificent option for the publication and review of high-level medical articles and broad collective interest. I am honored to be able to share my review article and open to receive all your comments.
“The peer review process of JPMHC is quick and effective. Authors are benefited by good and professional reviewers with huge experience in the field of psychology and mental health. The support from the editorial office is very professional. People to contact to are friendly and happy to help and assist any query authors might have. Quality of the Journal is scientific and publishes ground-breaking research on mental health that is useful for other professionals in the field”.
Dear editorial department: On behalf of our team, I hereby certify the reliability and superiority of the International Journal of Clinical Case Reports and Reviews in the peer review process, editorial support, and journal quality. Firstly, the peer review process of the International Journal of Clinical Case Reports and Reviews is rigorous, fair, transparent, fast, and of high quality. The editorial department invites experts from relevant fields as anonymous reviewers to review all submitted manuscripts. These experts have rich academic backgrounds and experience, and can accurately evaluate the academic quality, originality, and suitability of manuscripts. The editorial department is committed to ensuring the rigor of the peer review process, while also making every effort to ensure a fast review cycle to meet the needs of authors and the academic community. Secondly, the editorial team of the International Journal of Clinical Case Reports and Reviews is composed of a group of senior scholars and professionals with rich experience and professional knowledge in related fields. The editorial department is committed to assisting authors in improving their manuscripts, ensuring their academic accuracy, clarity, and completeness. Editors actively collaborate with authors, providing useful suggestions and feedback to promote the improvement and development of the manuscript. We believe that the support of the editorial department is one of the key factors in ensuring the quality of the journal. Finally, the International Journal of Clinical Case Reports and Reviews is renowned for its high- quality articles and strict academic standards. The editorial department is committed to publishing innovative and academically valuable research results to promote the development and progress of related fields. The International Journal of Clinical Case Reports and Reviews is reasonably priced and ensures excellent service and quality ratio, allowing authors to obtain high-level academic publishing opportunities in an affordable manner. I hereby solemnly declare that the International Journal of Clinical Case Reports and Reviews has a high level of credibility and superiority in terms of peer review process, editorial support, reasonable fees, and journal quality. Sincerely, Rui Tao.
Clinical Cardiology and Cardiovascular Interventions I testity the covering of the peer review process, support from the editorial office, and quality of the journal.
Clinical Cardiology and Cardiovascular Interventions, we deeply appreciate the interest shown in our work and its publication. It has been a true pleasure to collaborate with you. The peer review process, as well as the support provided by the editorial office, have been exceptional, and the quality of the journal is very high, which was a determining factor in our decision to publish with you.
The peer reviewers process is quick and effective, the supports from editorial office is excellent, the quality of journal is high. I would like to collabroate with Internatioanl journal of Clinical Case Reports and Reviews journal clinically in the future time.
Clinical Cardiology and Cardiovascular Interventions, I would like to express my sincerest gratitude for the trust placed in our team for the publication in your journal. It has been a true pleasure to collaborate with you on this project. I am pleased to inform you that both the peer review process and the attention from the editorial coordination have been excellent. Your team has worked with dedication and professionalism to ensure that your publication meets the highest standards of quality. We are confident that this collaboration will result in mutual success, and we are eager to see the fruits of this shared effort.
Dear Dr. Jessica Magne, Editorial Coordinator 0f Clinical Cardiology and Cardiovascular Interventions, I hope this message finds you well. I want to express my utmost gratitude for your excellent work and for the dedication and speed in the publication process of my article titled "Navigating Innovation: Qualitative Insights on Using Technology for Health Education in Acute Coronary Syndrome Patients." I am very satisfied with the peer review process, the support from the editorial office, and the quality of the journal. I hope we can maintain our scientific relationship in the long term.
Dear Monica Gissare, - Editorial Coordinator of Nutrition and Food Processing. ¨My testimony with you is truly professional, with a positive response regarding the follow-up of the article and its review, you took into account my qualities and the importance of the topic¨.
Dear Dr. Jessica Magne, Editorial Coordinator 0f Clinical Cardiology and Cardiovascular Interventions, The review process for the article “The Handling of Anti-aggregants and Anticoagulants in the Oncologic Heart Patient Submitted to Surgery” was extremely rigorous and detailed. From the initial submission to the final acceptance, the editorial team at the “Journal of Clinical Cardiology and Cardiovascular Interventions” demonstrated a high level of professionalism and dedication. The reviewers provided constructive and detailed feedback, which was essential for improving the quality of our work. Communication was always clear and efficient, ensuring that all our questions were promptly addressed. The quality of the “Journal of Clinical Cardiology and Cardiovascular Interventions” is undeniable. It is a peer-reviewed, open-access publication dedicated exclusively to disseminating high-quality research in the field of clinical cardiology and cardiovascular interventions. The journal's impact factor is currently under evaluation, and it is indexed in reputable databases, which further reinforces its credibility and relevance in the scientific field. I highly recommend this journal to researchers looking for a reputable platform to publish their studies.
Dear Editorial Coordinator of the Journal of Nutrition and Food Processing! "I would like to thank the Journal of Nutrition and Food Processing for including and publishing my article. The peer review process was very quick, movement and precise. The Editorial Board has done an extremely conscientious job with much help, valuable comments and advices. I find the journal very valuable from a professional point of view, thank you very much for allowing me to be part of it and I would like to participate in the future!”
Dealing with The Journal of Neurology and Neurological Surgery was very smooth and comprehensive. The office staff took time to address my needs and the response from editors and the office was prompt and fair. I certainly hope to publish with this journal again.Their professionalism is apparent and more than satisfactory. Susan Weiner
My Testimonial Covering as fellowing: Lin-Show Chin. The peer reviewers process is quick and effective, the supports from editorial office is excellent, the quality of journal is high. I would like to collabroate with Internatioanl journal of Clinical Case Reports and Reviews.
My experience publishing in Psychology and Mental Health Care was exceptional. The peer review process was rigorous and constructive, with reviewers providing valuable insights that helped enhance the quality of our work. The editorial team was highly supportive and responsive, making the submission process smooth and efficient. The journal's commitment to high standards and academic rigor makes it a respected platform for quality research. I am grateful for the opportunity to publish in such a reputable journal.
My experience publishing in International Journal of Clinical Case Reports and Reviews was exceptional. I Come forth to Provide a Testimonial Covering the Peer Review Process and the editorial office for the Professional and Impartial Evaluation of the Manuscript.
