AUCTORES
Review Article | DOI: https://doi.org/10.31579/2693-4779/213
Division of Biology/Biochemistry, University of Orléans, France.
*Corresponding Author: Michel Leclerc, Division of Biology/Biochemistry, University of Orléans, France.
Citation: Michel Leclerc, (2024), Complementary-Determining Region Invertebrate Primitive Antibody From;Sea Star »Modelization 3d With Human Igk, Clinical Research and Clinical Trials, 10(4); DOI:10.31579/2693-4779/213
Copyright: © 2024, Michel Leclerc. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Received: 18 June 2024 | Accepted: 05 July 2024 | Published: 16 August 2024
Keywords: artificial intelligence; urology; medical training; learning; technological advances
Our findings consist of the importance of an individualized approach based on infectious risk and prudent antibiotic management to prevent antibiotic resistance, and highlight the need for further research to better assess the clinical impact of antibiotic prophylaxis in this specific context of transurethral resection of bladder tumor.
10 years ago, we tried to clone, for the first time, the Asterias rubens sea star IGKappa gene by the use and the help of E.coli as amplificator [1]. It allowed, in à second time, to verify that the Young Protein, or anti-HRP Protein recognizes the HRP antigen [1,2]
In the present work we research Complementary Determining Regions called more briefly CDR1, CDR2, CDR3. Or Complementary-Determining Regions[3,4]
First, anti-HRP sequence in nucleotids is given :
5’GGA TCC GGA GGA ATG CGTGGCAACATGGCGTCTCTATGGATGTTCTTCTT
TGTCGTGGGGATAACTTTACAACGGAGTTTGGCGATTTACACGTTTCGCG
AGCAACCGTCGGACACTAGCGCGTTGCAGGGGAGCACAGTGGTGCTTCAC
TGCTCCGTTGAGCAGTACATAAACACCACGGCCATCGTTTGGTGGAGCCG
TGACTCGGTCATCAGCCACAACAAAGACCTGAAACTGTCCAGTCTAAACA
CCGACCAGCTCCAAAGGTACTCGATTTCAGGCGACGCATCTCGGGGGGAA
TTCAACCTTAAAATAGTGAACTTTACCGCCACAGACGCCGCCAGTTACCG
CTGTCAGATG TAA GAA TTC3’
with the tranlation https://web.expasy.org/translate/
gga tcc gga gga atg cgt ggc aac atg gcg tct cta tgg atg ttc ttc ttt gtc gtg ggg
G S G G M R G N M A S L W M F F F V V G
ata act tta caa cgg agt ttg gcg att tac acg ttt cgc gag caa ccg tcg gac act agc
I T L Q R S L A I Y T F R E Q P S D T S
gcg ttg cag ggg agc aca gtg gtg ctt cac tgc tcc gtt gag cag tac ata aac acc acg
A L Q G S T V V L H C S V E Q Y I N T T
gcc atc gtt tgg tgg agc cgt gac tcg gtc atc agc cac aac aaa gac ctg aaa ctg tcc
A I V W W S R D S V I S H N K D L K L S
agt cta aac acc gac cag ctc caa agg tac tcg att tca ggc gac gca tct cgg ggg gaa
S L N T D Q L Q R Y S I S G D A S R G E
ttc aac ctt aaa ata gtg aac ttt acc gcc aca gac gcc gcc agt tac cgc tgt cag atg
F N L K I V N F T A T D A A S Y R C Q M
taa gaa ttc
- E F .
OR in ANOTHER WAY :
MRGNMASLWMFFFVVGITLQRSLAIYTFREQPSDTSALQGSTVVLHCSVEQYINTTAIVWWSRDSVISHNKDLKLSSLNTDQLQRYSISGDASRGEFNLKIVNFTATDAASYRCQMFA
2 tables issued from IMGT resume the following analysis below : I):
https://www.imgt.org/3Dstructure-DB/cgi/DomainGapAlign.cgi with default settings, 17/01/2024
IMGT/DomainGapAlign version : 4.10.3 (2021-12-06)
II) Table II:Alignments :
>starfish|IGKV1-5*01|33.3|||Pongo abelii
…EQPSDTSALQGSTVVLHCSVEQYI.....NTTAIVWWSRDSVISHNKDL.KL.......SSLNTDQL.QRYSISGDASRGEFNLKIVNFTATDAASYRCQ.......................
The conserved amino acids (positions 23, 41, 89, 104) are found in the starfish sequence.
This molecule appears to have an IG AA sequence as seen from the above analysis.
1. If it aligns with the Pongo IGKV1-5, the percentage of alignment is 33%, so it is a sequence that seems to have similarities to an IGKV gene when it comes to conserved amino acids.
It appears clearly that CDR1 and CDR2 exist in the sea star primitive antibody and less clearly for CDR3 [1] amino acid which is conserved)
Undoubtly :
These new parameters corroborate the existence of an Invertebrate Primitive Antbody and NOT IG-LIKE as it is often said. We recall also the discovery by us of T and B sea star lymphocytes [5] Humoral specific response [6] Genomic data [7] with specially Invertebrate MHC genes
ALL these elements assess the existence of an IPA : Invertebrate Primitive Antibody which shares strong sequence alignments(at least for CDR1 and CDR2) with the Primate : Pongo pygmaeuserences . More recently, in a work concerning Modelizations in 3D of the sea star anti-HRP protein, we found a CDR3 region (see below this modelization when compared to AlphaFold prediction of IGKV1-5 03 from Homo sapiens (Figure.1)
Figure 1 : Alfold prediction of IGKV1-5*03 Homo sapiens
In black sea star CDR1, CDR2, CDR3(at the left) determining regions