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Research Article | DOI: https://doi.org/10.31579/2692-9406/156
1 Department of pharmaceutics, RBVRR Women’s college of pharmacy, Affiliated to Osmania University, Hyderabad.
*Corresponding Author: A.Krishna Sailaja, Department of pharmaceutics, RBVRR Women’s college of pharmacy, Affiliated to Osmania University, Hyderabad.
Citation: A.Krishna Sailaja and Jyoti S. Rajput (2023), Aspirin Loaded Nanosuspension Using Probe Sonication Method, J. Biomedical Research and Clinical Reviews. 8(3); DOI:10.31579/2692-9406/156
Copyright: © 2023, A.Krishna Sailaja. this is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Received: 14 June 2023 | Accepted: 21 June 2023 | Published: 28 June 2023
Keywords: asprin; nanosuspension; polaxamer; drug content; entrapment efficiency
Aspirin inhibit the activity of the enzyme now called cyclooxygenase (COX) which leads to the formation of prostaglandins (PGs) that cause inflammation, swelling, pain and fever. Aspirin having various function like anti-inflammatory, analgesic, antipyretic etc. Aspirin loaded nanosuspension was prepared to enhance the bioavailability of the drug. Nanosuspension is defined as a finely dispersed solid particle dissolved in aqueous and organic medium for oral, tropical, parenteral or pulmonary administration. The size ranges about 200nm to 600nm.Aspirin loaded nanosuspension was prepared by probe sonication method. The probe sonication method is used to reduce the particle size and to increase the solubility of the drug. Total 5 Nano formulations were prepared in which NS1 shows 100% of drug content. Invitro Drug release was performed using Franz diffusion cell in which NS5 formulation shows 94.7% drug release in a time period of two hours and 30 minutes. Entrapment efficiency was performed using Ultracentrifugation method kept for 40 minutes at 17,000 rpm speed. Among all NS5 shows 71.9% of entrapment efficiency
Parenteral or pulmonary administration. The size ranges about 200nm to 600nm.Nanosuspension is having several advantage like it will improve the solubility issues of the drug and also improve bioavailability. Aspirin having anti-inflammatory action by inhibiting cyclooxygenase which will prevent the synthesis of prostaglandins. Aspirin is rapidly deacetylated by esterasein the body there by producing salicylate which has anti-inflammatory, antipyretic and analgesic effect. Aspirin loaded nanosuspension is prepare by using probe sonication method to improve solubility of the drug and reduce dosage regimen [1-3].
Advantages:
Disadvantages:
Table no :1
Preparation
Method: Probe Sonication method
Aspirin loaded nanosuspension was prepared by probe sonication method. Drug [10mg] was dissolved in a aqueous solution consisting of surfactant [20,40,60,80,100mg]. Then subjected to stirring until drug completely dissolves. The prepared suspension was subjected to probe sonication.
Characterization of Nanosuspension
Table no: 2 Stability issues of Nanosuspension
Evaluation test
1] Drug content: Take 1ml of suspension dissolved in 10 ml of volumetric flask make up the volume up to 10ml with methanol. Then check for the absorbance under UV spectrometer.
2] Entrapment efficiency : 1lml of suspension was taken dissolved in 9 ml of 7.4 phosphate bufferkept for ultracentrifugation for 45 minutes at 17,000 rpm speed. Thesupernatant was collected and check under UV spectrophotometry.
3] Dissolution study: The dissolution study was carried out by Franz diffusion cell. In which it consist of 2 compartment one is donar and another is receptor .The membrane was placed in between donar and receptor compartment. The sample was withdrawn at every regular interval and check under UV.
4] Determination of nanosuspension particle yield: The product yield was calculated by gravy metery [11-13].
1] Calibration curve: The calibration curve of Aspirin was performed by taking methanol as solvent.
Figure 1 calibration curve of Aspirin
The plot was showing linearity with a regression value of 0.973 as shown in figure no 1 2] Drug content: the Drug content was carried out by taking 5 ml of nanosuspension formulation in which NS1 shows 100% of drug content as shown in figure no 2
Figure 2 Drug content of nanosuspension formulations
3] Entrapment efficiency: The entrapment efficiency was carried out by ultracentrifugation in which NS5 shows 71.9%
Figure 3: Entrapment efficiencies of nanosuspension formulations
4] Dissolution Study: In vitro drug release was obtained by using Franz diffusion cell in which NS5 formulation showed 94% of drug release in 150 minute of time interval as shown in figure no 4
Figure 4: In-vitro dissolution studies of nanosuspension formulations
Aspirin loaded nanosuspension were prepared by probe sonication method. In which 5 Nanoformulation were prepare and evaluation test was carried out. The NS1 shows 100% of drug content. The entrapment efficiency was performed by using probe sonication method in which NS5 shows 71.9% of entrapment. In vitro drug release were done by using Franz diffusion cell NS5 formulation showed 94.7 % of drug release in 2.30 minutes of time interval.Aspirin loaded nanosuspension will enhance bioavailability of drug and its solubility profile .The probe sonication method is used to reduce the particle size and increase the solubility of the drug.
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