AUCTORES
Globalize your Research
Research Article | DOI: https://doi.org/10.31579/ cobs.18/001
*Corresponding Author: Randy J. Seeley, PhD, Department of Surgery, University of Michigan, Ann Arbor, MI, USA. E-mail: seeleyrj@med.umich.edu
Citation: Elika Ridelman, Simon S. Evers, Mark J. Hoenerhoff, Jeffrey E. Green, Randy J. Seeley, Alterations in Serum-based Breast Cancer Markers Following Bariatric Surgery in rodent models: Are They of Clinical Value?, Clinical Obesity and Bariatric surgery.Doi: http://dx.doi.org/ 10.31579/ cobs.18/001.
Copyright: © 2018 Randy J. Seeley. This is an open-access article distributed under the terms of the Creative Commons Attribution License,which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Received: 14 August 2018 | Accepted: 20 August 2018 | Published: 23 August 2018
Keywords: vertical sleeve gastrectomy (VSG); bariatric surgery; breast cancer
Objective: Breast cancer is one of the most common malignancies in females worldwide. The percentage of adults with a body-mass index (BMI) of 25 kg/m2 or greater is 38.0% in U.S. females. The increase in obesity epidemic in females is accompanied with an increase in bariatric procedures that not only lower weight but appear to lower risks of breast cancer. The objective of this paper is to explore the effects of bariatric surgery on multiple serum-based breast tumor markers in breast cancer rodent models.
Research design and methods: 40 total mice were studied in C3Tag (1) transgenic model of breast cancer. Half of animals went through vertical sleeve gastrectomy (VSG) (one group on 60% HFD and one group on Chow) and were compared the other half undergoing sham surgery. Data collection was performed ~6 weeks post surgeries for both cohorts.
Results: Previously, our group has demonstrated positive impacts with VSG on glucose homeostasis, cholesterol, and body composition compared to sham treated animals. This study was performed to explore the serum growth factor alterations following bariatric surgeries. 6 weeks post-surgery, we observed changes in serum-based biomarkers post bariatric surgery.
Conclusions: Bariatric surgery may provide improvements in growth factor serum levels that might be contributors to reduced breast cancer risk.
Limitations: This study is limited by small sample size and advanced breast cancer in an aggressive tumorigenesis rodent model. Further study is warranted.
The prevalence of obesity continues to rise at a significant pace in most developed countries [1]. Long term consequences of obesity include cardiovascular complications and diabetes-related morbidity and mortality [2]. However, obesity more recently has been recognized as an important risk factor for the development of cancer, including breast cancer in women [3]. Strikingly, there is accumulating evidence that demonstrates that obesity is directly related to increased morbidity and mortality following cancer diagnosis [4]. Accomplishing a reduction in body weight may therefore be beneficial in reducing the risk of developing cancer but may also improve treatment outcomes following diagnosis. The most effective current strategy to induce weight loss is bariatric surgery. The most universally implemented bariatric surgeries include: open or laparoscopic Roux-en-Y gastric bypass (RYGB) and vertical sleeve gastrectomy (VSG) [5]. These surgeries result in significant improvements in glucose and insulin profiles, with some patients being able to discontinue type 2 diabetes treatment only a few days after surgery [6].
Besides the well-documented improvements in cardiovascular health and glucose metabolism related to surgery-induced weight loss, these surgeries have also been related to a reduction in the incidence of breast cancer [7, 8]. The question remains if this reduction in breast cancer occurrence is related to weight loss alone, or if there are other mechanisms triggered following these surgeries that may be detrimental to tumor growth. Weight loss following bariatric surgery was thought to be the result of mechanically restricting nutrient intake and absorption. However, currently it is accepted that the weight-loss and metabolic improvements induced by bariatric surgery are also caused by physiological changes of the intestinal tract [9]. Some of the physiological impacts of the surgery include changes in GI function and morphology [10], changes in secreted gut peptides such as ghrelin, GLP-1, GLP-2 and cholecystokinin (CCK) [11], bile acid levels and composition [12] and even rearrangements of microbial composition [13, 14].
Recently it has been shown that bariatric surgery reduces the inflammatory state as well as a reduction in local hypertrophy of the adipose tissue in rodents [15]. Adipocyte hypertrophy resulted from obesity leads to systemic inflammation [16, 17]. Several lines of evidence suggest that inflammation has crucial roles to the initiation, promotion, and progression and metastasis in breast cancer [18, 19]. It is suggested that the systematic inflammation, as well as the local inflamed adipose tissue of the breast could form a niche to promote a favorable environment for breast cancer [20]. Together these effects could result in lowering breast cancer risk in obese women.
Therefore, one possibility is that surgery could reduce inflammation and circulating markers of inflammation that include a variety of growth factors. In the studies described here, we performed VSG in a transgenic rodent model destined to develop breast cancer. In this model, atypia of the mammary ductal epithelium develops at 8weeks of age, and mammary intraepithelial neoplasia (MIN) are progressed by 12 weeks. Invasive and metastatic carcinomas develop in 100% of animals by 16 weeks of age [21]. This model is an established model of mammary and prostate cancers similar to human cancers. Here we measured a variety of serum-based endpoints to determine whether they are reduced after VSG. We also performed SCFA quantification through gas chromatography, in order to identify the potential benefits of VSG on gut bacteria by products.
Methods
Animals
For all experiments we used the C3Tag (1) mouse model. The breeding animals were donated to the Seeley lab from Jeffrey E Green at the National Institute of Health (NIH). Invasive and metastatic carcinomas develop in 100% of animals by 16 weeks of age. Two cohorts of age-matched (6 weeks) C3Tag (1) female mice (Cohort one: N=30; Cohort two: N=29), body weight (22.46 +/- 2.3 g at arrival; Jeffrey E Green Laboratories, NIH, Bethesda, MD) were individually housed and maintained on a 12/12-h light/dark cycle at 25°C and 50–60% humidity. The initial total number of animals in cohort one was sixty and in cohort two was forty. In cohort one 12 animals and in cohort two 7 animals were sacrificed due to over limit tumor size before the termination point. Twenty-two animals died because of unknown reasons, likely due to surgery complications. Animals were matched by age because of the complications involved in matching the tumor developing age required for experiments’ time points. Further, animals were randomized based on their body mass to minimize complicating different weight gain trajectories in response to 60% high-fat diet. Following acclimatization to the facilities, animals had ad libitum access to water and standard chow (manufacturer) or a palatable 60% high-fat diet (D12451, Research Diets, New Brunswick, NJ) for 2 weeks prior to surgery and maintained on the diet until the studies were terminated. Animals were assigned to receive either Sham or VSG surgery in a counterbalanced fashion by body weight. All procedures for animal use were approved by the University of Michigan Animal Care and Use Committee (Protocol number: PRO00008242) and follow the guidelines outlined in the National Institutes of Health guide for the care and use of laboratory animals (NIH Publications No. 8023, revised 1978).
Surgical procedures
Four days prior to surgery, body composition was assessed using an EchoMRITM analyzer (EchoMRI LLC, Houston, TX). Solid food was removed 24hrs prior to surgery and mice got ad lib access to a liquid diet (Osmolite 1.0 Cal, Abbott). Here we describe both surgical procedures in details:
Vertical Sleeve Gastrectomy
Animals were anesthetized by isoflurane inhalation and received subcutaneous injections of buprenorphine hydrochloride (0.1 mg/kg) and meloxicam (0.5 mg/kg). A midline abdominal skin incision is made, followed by an incision in the underlying muscle. The stomach is exposed and transected to form a sleeve. Sleeve formation consists of the removal of 80% of the stomach using an ETS 35-mm stapler (Ethicon Endo-Surgery), leaving behind 20% of the stomach which is a tubular gastric “sleeve”. The sleeve portion of the stomach is placed back into the abdominal cavity and the muscle and skin of abdominal wall are sutured in separate layers.
Sham Surgery
Animals were anesthetized by isoflurane inhalation and received subcutaneous injections of buprenorphine hydrochloride (0.1 mg/kg) and meloxicam (0.5 mg/kg). An abdominal laparotomy was performed, light manual pressure was applied with to the exteriorized stomach, and then the abdomen was closed in layers. After surgery, animals are put on a nutritionally complete Osmolite liquid diet which is replaced with pre-surgery solid diets on the fourth day post-operation. Post-surgical analgesia consisted of daily subcutaneous injection of an NSAID (e.g. Meloxicam) for at least 3 days.
Body weight and body composition
Measurements were collected in two cohorts for these studies. Cohort one refers to the first cohort where the main purpose was the growth factor measurements after VSG. Second cohort was used mainly for the SCFA measurements and is referred to cohort two in this paper. In all cohort of VSG and sham animals, body weights were measured daily for the first week following surgery as a post-op care routine and once weekly for the duration of the study. Body composition was determined in live animals 4 days prior to surgery, and then cohort one 5 weeks and cohort two 4 weeks following surgery via EchoMRI™ Analyzers.
Preparation of Plasma Samples
Plasma were collected in mice upon sacrificing. Plasma collection was performed using EDTA as an anti-coagulant. Samples were centrifuged for 10 minutes at 1000xg within 30 minutes of blood collection. Plasma was removed and aliquots were made to reduce freeze/thaw cycles for further analysis. Samples were stored at ≤ -80°C.
Biomarker Quantification
To identify specific hormones that change following bariatric surgeries in animal models high at risk of breast cancer, it was necessary to screen panels of growth factor hormones. Biomarker quantifications was performed by Luminex 200™ System and Luminex XY Platform™. Simultaneous quantification of several analyses was performed according to manufacture protocol in serum samples (Luminex®).
High-sensitivity detection of short-chain fatty acids in colon by gas chromatography-mass spectrometry
High-sensitivity detection of SCFA in colon was measured using the gas chromatography Agilent 69890N GC -5973 MS detector.
1) Sample preparation: ~50 mg of tissue was transferred to a pre-weighed locked Eppendorf tube and 600 uL of 30 mM hydrochloric acid containing isotopically-labeled acetate (150uM), propionate (75uM), butyrate, isobutyrate, valerate (30 uM), isovalerate, hexanoate, heptanoate and octanoate (15 uM) was added to each sample. Samples were homogenized in a Bullet Blender Gold at speed 8 for 5 minutes. Samples were vortexed for 10 seconds, incubated for 10 minutes on wet ice, and then vortexed again for 10 seconds. Samples were then centrifuged at 1500 rcf, 4 °C for 10 minutes and the supernatant transferred to a 1 mL glass tube. 20 uL of each sample was removed to a separate glass vial to create a pooled sample for QC purposes. 300 uL of methyl tert-butyl ether (MTBE) was added to each sample and the mixture vortexed for 10 seconds to emulsify, then held at 4 °C for 5 minutes, and vortexed again for 10 seconds. Samples were centrifuged for 1 minute to separate the solvent layers and the MTBE layer was then removed to an autosampler vial for GC-MS analysis. A series of calibration standards were prepared along with samples to quantify metabolites. Post-extract, tissues were taken to dryness in speedvac and the Eppendorf tube re-weighed to obtain the dry tissue weight for normalization.
2) GC-MS analysis: GC-MS analysis was performed on an Agilent 69890N GC -5973 MS detector with the following parameters: a 1µL sample was injected with a 1:10 split ratio on a ZB-WAXplus, 30m x0.25mmx0.25um (Phenomenex Cat#7HG-G013-11) GC column, with him as the carrier gas at a flow rate: 1.1ml/min. The injector temperature was 240 °C, and the column temperature was isocratic at 310 °C.
3) SCFAs Data analysis: Data were processed using Mass Hunter Quantitative analysis version B.07.00. SCFAs were normalized to the nearest isotope labeled internal standard and quantitated using 2 replicated injections of 5 standards to create a linear calibration curve with accuracy better than 80% for each standard. Data were processed using Mass Hunter Quantitative analysis version B.07.00. Metabolites in the glycolysis/tca/ppp pathways were normalized to the nearest isotope labeled internal standard and quantitated using 2 replicated injections of 5 standards to create a linear calibration curve with accuracy better than 80% for each standard. Other compounds in the analysis were normalized to the nearest internal standard, and the peak areas were used for differential analysis between groups. Samples were normalized to dry sample weight after quantification.
Statistical analysis
Data are expressed as average ± standard error of the mean (SEM). Between groups differences were analyzed for statistical significance using one-way ANOVA testing combined with post hoc Tukey when appropriate. For time dependent analysis repeated measures (RM) ANOVA were performed. Differences between data were considered statistically significant when P<0.05. Statistical tests were performed in IBM SPSS v.23, all graphs were created in Graph pad Prism 7.0.
Clinical data suggest that bariatric surgery reduces breast cancer risk in females [22]. To study the possible relation between VSG and carcinogenesis, two separate cohorts of the rodent model of breast cancer C3Tag (1) were generated to test the influence of VSG on 1) the composition of circulating biomarkers related to cancer in this cancer model (cohort one) and 2) the short chain fatty acid composition of the colon (cohort two). C3Tag (1) transgenic mice overexpress the 5' flanking region of the C3 (1) component of the rat prostate steroid binding protein (PSBP) and targets the expression of the SV40 large T-antigen (Tag) to the epithelium of mammary glands.
Cohort One: Effects of bariatric surgery on circulating levels of growth factor breast cancer markers
A. Body weight and composition
C3Tag (1) mice fed a high fat diet seem to be unresponsive to VSG at the level of long-term body weight regulation. C3Tag (1) mice fed a chow diet combined with VSG are lower in body weight over the duration of the study (fig 1a), but the body weight over time is only significantly different from the Sham-HFD group (P<0.01, F34, 459=2.282, rm-ANOVA post hoc Tukey). As depicted in figure 1A, actual body weights in the VSG-Chow group are lower compared to the Sham-HFD group at days -7, 0, 1-7, 28, 49, 63, and 70 post-surgery (*P<0.05, **P<0.01, one-way ANOVA post hoc Tukey).
Remarkably, only at day 70 post-surgery actual body weight between the chow and HFD fed VSG groups was significantly different (^P<0.05, post hoc Tukey). When body weight over time was tested for main effects, both an effect of surgery (P<0.01, F17, 459=2.444, rm-ANOVA) as well as an effect of diet (P<0.001, F17, 459=2.505, rm-ANOVA) was found. In contrast, relative body weight dynamics following surgery over the duration of the study did not differ between groups (rm-ANOVA post hoc Tukey; fig. 1B). This suggests that actual body weight differences are primarily a result of the initial lower body weight of the chow fed mice at the moment of surgery. This difference in initial body weight is most likely a result of reduced adipose tissue mass in the Chow fed mice prior to surgery (Fig. 1C) compared to both HFD fed groups (^P<0.05, F2, 31=5.354, one-way ANOVA post hoc Tukey), whereas at 5 weeks post-surgery adiposity levels in the VSG-Chow group were only reduced compared to the Sham-HFD group (P<0.01, F2, 31=7.978, One-way ANOVA post hoc Tukey). Nonetheless a general reduction in adiposity levels was observed in the Chow-VSG group compared to both the Sham-HFD and VSG-HFD group (P<0.001, F2, 32=11.344, rm-ANOVA post hoc Tukey).
B. Growth factors
Growth factors regulate development of the normal breast as well as the carcinogenesis of epithelium breast tissue. To assess the effects of bariatric surgery on circulating growth factors, we analyzed several of these factors that are the key mediators of breast cancer growth in serum from animals in each surgery group.
As depicted in figure 2, leptin levels (fig. 2A) were reduced in the VSG-Chow group compared to Sham-HFD (P<0.05, F2,27=5.095, one-way ANOVA post hoc Tukey), additionally a main effect of surgery was observed for reduced leptin levels following VSG (P<0.05, F1,27=3.518, glm-ANOVA). Compared to Sham-HFD, PlGF-2 levels (fig. 2B) were decreased in both the VSG-Chow (P<0.01) and VSG-HFD (P<0.05, F2,28=6.390, one-way ANOVA post hoc Tukey), additionally a main effect of surgery was observed for reduced levels of PlGF-2 in the VSG groups (P<0.05, F1,28=7.648, glm-ANOVA). Likewise, endoglin levels (fig. 2C) were reduced in both the VSG-Chow (P<0.01) and VSG-HFD (P<0.05, F2,29=18.744, one-way ANOVA post hoc Tukey) groups compared to the Sham-HFD group, resulting in a main effect of surgery (P<0.001, F1,29=33.453, glm-ANOVA). In contrast, circulating G-CSF levels (fig. 2D) were increased in the VSG-Chow (P<0.01) and VSG-HFD (P<0.05, F2,28=6.390, one-way ANOVA post hoc Tukey) compared to the Sham-HFD group, resulting in a main effect of surgery (P<0.01, F1,28=8.070, glm-ANOVA).
Table 1 shows the average circulating levels of all biomarkers tested and additionally shows a main effect of surgery for increased levels of Follistatin (P<0.05, F1,28=4.969, glm-ANOVA), whereas VSG resulted in a trend of increased levels of both IL-6 (P=0.07) and Prolactin (P=0.08).
Cohort Two: Colon derived short chain fatty acid composition is altered following vertical sleeve gastrectomy.
A. Body weight and body composition
VSG surgery induced an initial reduction in body weight compared to Sham that was completely re-gained at day 14 post-surgery and lasted throughout the rest of the study (Fig. 3A). Nonetheless, repeated measures analysis did measure a significant difference between the body weight dynamics over time between VSG and Sham (P<0.001, F10,270=6.641, rm-ANOVA). Between group analysis revealed that actual body weight was only lower at post-surgery days 1 to 7 (one-way ANOVA). Similarly, the overall relative weight changes post-surgery (fig. 3B) was different between groups (P<0.001, F10,270=8.960, rm-ANOVA) and relative body weight was lowered at days 1 to 6 post-surgery (one-way ANOVA) in the VSG group. Likewise, relative weight loss in the VSG was re-gained at day 7 post-surgery and persisted over time. Adipose tissue mass was assessed before and 4 weeks post-surgery. No differences between groups were observed for total adiposity (Fig. 3C).
B. Detection of short-chain fatty acids in colon
Short-chain fatty acids (SCFAs) are produced in the colon by the gut microbiome as a result of dietary fiber fermentation [23]. SCFAs play crucial roles in health and disease states and have been implicated in some types of cancers [24]. In particular, butyrate has been associated with breast carcinogenesis [25]. No significant differences between Sham and VSG were observed for the levels of butyrate, acetate, propionate, octanoate, heptanoate and valerate in the colon (see Table 2).
However, levels of iso-valerate were significantly higher in the colon of VSG-treated mice compared to Sham (P<0.05, F1,26=5.370, one-way ANOVA) (Fig. 4).
VSG in mice that are genetically prone to develop mammary gland carcinomas did not significantly affect the occurrence or severity of tumor development in these mice. One explanation may be that the aggressiveness of the tumor development due to the genetic modification is too powerful to be overcome by surgical intervention or reduced adiposity due to a standard chow diet. In short, whatever one does, these mice will develop carcinomas. More remarkable, the VSG did not result in additional weight loss, therefore the weight loss that may have reduced the spontaneous cancer development could also not be achieved. The reason that the VSG did not induce additional weight loss may be partly explained by the occurrence of tumor growth in these mice and therefor all mice, including the Sham, are lean and don’t have much excess weight to lose. Another reason may be that the background of the C3Tag (1) mice is FVBN, which is a mouse line known to be resistant to diet induced obesity [26]. That being said, the chow fed mice were leaner compared to their HFD fed VSG counterparts, so there could be another physiological mechanism in this strain that makes it resistant to surgery-induced weight loss. Fortunately, this provided the opportunity to study the weight-independent effects of surgery on circulating biomarkers and SCFAs.
In both cohorts, there was no significant surgery effects on weights between VSG-HFD groups compared to sham-HFD (Figure 1 and 3). This might strike some as surprising but the background strain of these C3Tag mice are FVBN. This strain is quite resistant to weight gain when exposed to a HFD and consequently their starting body weights and body adiposity levels are much lower than in most work that has been done exposing mice to VSG [27]. The advantage of this observation is that any observed physiological effects of VSG are the product of the surgical effect and not secondary to weight-loss. All the animals were maintained on their respective diet after surgery, as a result the VSG- chow group weights was significantly lower than both HFD groups.
Although a number of factors influence an individual’s predisposition to breast cancer and course of progression to cancer, a growing body of evidence shows various effects of SCFAs on carcinogenesis [28, 29]. SCFAs are produced in the proximal colon through anaerobic bacteria substrate fermentation [30]. Approximately, 95% of the produced SCFAs are absorbed by the colonocytes and about 5% are lost in the feces [31, 32]. Some of the anti-cancer effects of SCFAs includes roles in differentiation, tumor growth arrest, and apoptosis [33]. In this study, we measured the SCFAs concentrations in intestinal colon after VSG in a mouse breast cancer model. There is no significant difference in the levels of primary SCFAs (propionate, acetate and butyrate). However, isovalerate concentrations were higher as a result of VSG. High levels of isovalerate suggest inadequate protein digestion.
Here, we confirm that circulating leptin levels were significantly lower after VSG. The reductions in circulating leptin levels are similar to the total adiposity levels. Several studies have shown a link between the levels of the adipocyte derived hormone leptin contributing to tumor formation and/or development [34-36]. Nonetheless, lower levels of leptin after VSG may contribute to breast cancer risk reduction.
We show a dramatic reduction of PlGF-2 levels post-VSG in both diet groups. Placenta growth factor-2 (PlGF-2) is an angiogenic protein belonging to the vascular endothelial growth factor (VEGF) family of growth factors and is upregulated mainly in pathologic conditions and cancer [37, 38]. PIGF-2 has been extensively researched clinically [39] and experimentally [40, 41] and its significance and roles in tumor progression is established [42].
Consistent with the decreased breast cancer risk observed after bariatric surgery, G-CSF levels are significantly higher after VSG both in HFD and chow. Growth factor families play crucial roles in tumor initiation, expansion, invasion and angiogenesis [43, 44]. Therefore, it is of high interest to observe that G-CSF levels are significantly higher due to VSG independent of diet. Granulocyte colony stimulating factor (G-CSF) stimulates white blood cell production in the bone marrow and contributes to the movement of stem cells from the bone marrow into the blood, strengthening the immune system [45]. The combination effects of G-CSF actions have been known to reduce inflammation and improve breast cancer therapies [46].
Conclusions
VSG results in dramatic shifts in circulating levels of growth factors and some of these effects appear to be a direct effect of VSG rather than a secondary effect of the resulting weight loss. These direct effects of surgery may contribute to the observed effect of bariatric surgery to reduce breast cancer risk and imply that some of the effects of surgery to alter breast cancer risk may not be over and above the potential benefit that accrues as a result of the substantial weight loss. Further work is needed to develop and determine the mechanisms that are responsible for alterations of SCFAs and growth factors after VSG.
Acknowledgments
1- The Michigan Diabetes Research Center (MDRC) Chemistry Laboratory, University of Michigan, Ann Arbor, MI, USA.
2- The Metabolomics Core, University of Michigan, Ann Arbor, MI,USA.
Competing Interests
R.J.S. serves as a paid consultant for and receives research support from Ethicon Endo-Surgery (a subsidiary of Johnson & Johnson), Orexigen, Novo Nordisk, Novartis, Daiichi Sankyo, Janssen (a subsidary of Johnson & Johnson), and Kallyope. He serves as a paid consultant for Novartis, and Scohia. R.J.F. receives research support from Zafgen, Sanofi, and MedImmune. In addition, R.J.S. is a paid-consultant and expert witness for Paul Hastings Law Firm. B.C.E.P. has declared no conflicts of interest.
Clearly Auctoresonline and particularly Psychology and Mental Health Care Journal is dedicated to improving health care services for individuals and populations. The editorial boards' ability to efficiently recognize and share the global importance of health literacy with a variety of stakeholders. Auctoresonline publishing platform can be used to facilitate of optimal client-based services and should be added to health care professionals' repertoire of evidence-based health care resources.
Journal of Clinical Cardiology and Cardiovascular Intervention The submission and review process was adequate. However I think that the publication total value should have been enlightened in early fases. Thank you for all.
Journal of Women Health Care and Issues By the present mail, I want to say thank to you and tour colleagues for facilitating my published article. Specially thank you for the peer review process, support from the editorial office. I appreciate positively the quality of your journal.
Journal of Clinical Research and Reports I would be very delighted to submit my testimonial regarding the reviewer board and the editorial office. The reviewer board were accurate and helpful regarding any modifications for my manuscript. And the editorial office were very helpful and supportive in contacting and monitoring with any update and offering help. It was my pleasure to contribute with your promising Journal and I am looking forward for more collaboration.
We would like to thank the Journal of Thoracic Disease and Cardiothoracic Surgery because of the services they provided us for our articles. The peer-review process was done in a very excellent time manner, and the opinions of the reviewers helped us to improve our manuscript further. The editorial office had an outstanding correspondence with us and guided us in many ways. During a hard time of the pandemic that is affecting every one of us tremendously, the editorial office helped us make everything easier for publishing scientific work. Hope for a more scientific relationship with your Journal.
The peer-review process which consisted high quality queries on the paper. I did answer six reviewers’ questions and comments before the paper was accepted. The support from the editorial office is excellent.
Journal of Neuroscience and Neurological Surgery. I had the experience of publishing a research article recently. The whole process was simple from submission to publication. The reviewers made specific and valuable recommendations and corrections that improved the quality of my publication. I strongly recommend this Journal.
Dr. Katarzyna Byczkowska My testimonial covering: "The peer review process is quick and effective. The support from the editorial office is very professional and friendly. Quality of the Clinical Cardiology and Cardiovascular Interventions is scientific and publishes ground-breaking research on cardiology that is useful for other professionals in the field.
Thank you most sincerely, with regard to the support you have given in relation to the reviewing process and the processing of my article entitled "Large Cell Neuroendocrine Carcinoma of The Prostate Gland: A Review and Update" for publication in your esteemed Journal, Journal of Cancer Research and Cellular Therapeutics". The editorial team has been very supportive.
Testimony of Journal of Clinical Otorhinolaryngology: work with your Reviews has been a educational and constructive experience. The editorial office were very helpful and supportive. It was a pleasure to contribute to your Journal.
Dr. Bernard Terkimbi Utoo, I am happy to publish my scientific work in Journal of Women Health Care and Issues (JWHCI). The manuscript submission was seamless and peer review process was top notch. I was amazed that 4 reviewers worked on the manuscript which made it a highly technical, standard and excellent quality paper. I appreciate the format and consideration for the APC as well as the speed of publication. It is my pleasure to continue with this scientific relationship with the esteem JWHCI.
This is an acknowledgment for peer reviewers, editorial board of Journal of Clinical Research and Reports. They show a lot of consideration for us as publishers for our research article “Evaluation of the different factors associated with side effects of COVID-19 vaccination on medical students, Mutah university, Al-Karak, Jordan”, in a very professional and easy way. This journal is one of outstanding medical journal.
Dear Hao Jiang, to Journal of Nutrition and Food Processing We greatly appreciate the efficient, professional and rapid processing of our paper by your team. If there is anything else we should do, please do not hesitate to let us know. On behalf of my co-authors, we would like to express our great appreciation to editor and reviewers.
As an author who has recently published in the journal "Brain and Neurological Disorders". I am delighted to provide a testimonial on the peer review process, editorial office support, and the overall quality of the journal. The peer review process at Brain and Neurological Disorders is rigorous and meticulous, ensuring that only high-quality, evidence-based research is published. The reviewers are experts in their fields, and their comments and suggestions were constructive and helped improve the quality of my manuscript. The review process was timely and efficient, with clear communication from the editorial office at each stage. The support from the editorial office was exceptional throughout the entire process. The editorial staff was responsive, professional, and always willing to help. They provided valuable guidance on formatting, structure, and ethical considerations, making the submission process seamless. Moreover, they kept me informed about the status of my manuscript and provided timely updates, which made the process less stressful. The journal Brain and Neurological Disorders is of the highest quality, with a strong focus on publishing cutting-edge research in the field of neurology. The articles published in this journal are well-researched, rigorously peer-reviewed, and written by experts in the field. The journal maintains high standards, ensuring that readers are provided with the most up-to-date and reliable information on brain and neurological disorders. In conclusion, I had a wonderful experience publishing in Brain and Neurological Disorders. The peer review process was thorough, the editorial office provided exceptional support, and the journal's quality is second to none. I would highly recommend this journal to any researcher working in the field of neurology and brain disorders.
Dear Agrippa Hilda, Journal of Neuroscience and Neurological Surgery, Editorial Coordinator, I trust this message finds you well. I want to extend my appreciation for considering my article for publication in your esteemed journal. I am pleased to provide a testimonial regarding the peer review process and the support received from your editorial office. The peer review process for my paper was carried out in a highly professional and thorough manner. The feedback and comments provided by the authors were constructive and very useful in improving the quality of the manuscript. This rigorous assessment process undoubtedly contributes to the high standards maintained by your journal.
International Journal of Clinical Case Reports and Reviews. I strongly recommend to consider submitting your work to this high-quality journal. The support and availability of the Editorial staff is outstanding and the review process was both efficient and rigorous.
Thank you very much for publishing my Research Article titled “Comparing Treatment Outcome Of Allergic Rhinitis Patients After Using Fluticasone Nasal Spray And Nasal Douching" in the Journal of Clinical Otorhinolaryngology. As Medical Professionals we are immensely benefited from study of various informative Articles and Papers published in this high quality Journal. I look forward to enriching my knowledge by regular study of the Journal and contribute my future work in the field of ENT through the Journal for use by the medical fraternity. The support from the Editorial office was excellent and very prompt. I also welcome the comments received from the readers of my Research Article.
Dear Erica Kelsey, Editorial Coordinator of Cancer Research and Cellular Therapeutics Our team is very satisfied with the processing of our paper by your journal. That was fast, efficient, rigorous, but without unnecessary complications. We appreciated the very short time between the submission of the paper and its publication on line on your site.
I am very glad to say that the peer review process is very successful and fast and support from the Editorial Office. Therefore, I would like to continue our scientific relationship for a long time. And I especially thank you for your kindly attention towards my article. Have a good day!
"We recently published an article entitled “Influence of beta-Cyclodextrins upon the Degradation of Carbofuran Derivatives under Alkaline Conditions" in the Journal of “Pesticides and Biofertilizers” to show that the cyclodextrins protect the carbamates increasing their half-life time in the presence of basic conditions This will be very helpful to understand carbofuran behaviour in the analytical, agro-environmental and food areas. We greatly appreciated the interaction with the editor and the editorial team; we were particularly well accompanied during the course of the revision process, since all various steps towards publication were short and without delay".
I would like to express my gratitude towards you process of article review and submission. I found this to be very fair and expedient. Your follow up has been excellent. I have many publications in national and international journal and your process has been one of the best so far. Keep up the great work.
We are grateful for this opportunity to provide a glowing recommendation to the Journal of Psychiatry and Psychotherapy. We found that the editorial team were very supportive, helpful, kept us abreast of timelines and over all very professional in nature. The peer review process was rigorous, efficient and constructive that really enhanced our article submission. The experience with this journal remains one of our best ever and we look forward to providing future submissions in the near future.
I am very pleased to serve as EBM of the journal, I hope many years of my experience in stem cells can help the journal from one way or another. As we know, stem cells hold great potential for regenerative medicine, which are mostly used to promote the repair response of diseased, dysfunctional or injured tissue using stem cells or their derivatives. I think Stem Cell Research and Therapeutics International is a great platform to publish and share the understanding towards the biology and translational or clinical application of stem cells.
I would like to give my testimony in the support I have got by the peer review process and to support the editorial office where they were of asset to support young author like me to be encouraged to publish their work in your respected journal and globalize and share knowledge across the globe. I really give my great gratitude to your journal and the peer review including the editorial office.
I am delighted to publish our manuscript entitled "A Perspective on Cocaine Induced Stroke - Its Mechanisms and Management" in the Journal of Neuroscience and Neurological Surgery. The peer review process, support from the editorial office, and quality of the journal are excellent. The manuscripts published are of high quality and of excellent scientific value. I recommend this journal very much to colleagues.
Dr.Tania Muñoz, My experience as researcher and author of a review article in The Journal Clinical Cardiology and Interventions has been very enriching and stimulating. The editorial team is excellent, performs its work with absolute responsibility and delivery. They are proactive, dynamic and receptive to all proposals. Supporting at all times the vast universe of authors who choose them as an option for publication. The team of review specialists, members of the editorial board, are brilliant professionals, with remarkable performance in medical research and scientific methodology. Together they form a frontline team that consolidates the JCCI as a magnificent option for the publication and review of high-level medical articles and broad collective interest. I am honored to be able to share my review article and open to receive all your comments.
“The peer review process of JPMHC is quick and effective. Authors are benefited by good and professional reviewers with huge experience in the field of psychology and mental health. The support from the editorial office is very professional. People to contact to are friendly and happy to help and assist any query authors might have. Quality of the Journal is scientific and publishes ground-breaking research on mental health that is useful for other professionals in the field”.
Dear editorial department: On behalf of our team, I hereby certify the reliability and superiority of the International Journal of Clinical Case Reports and Reviews in the peer review process, editorial support, and journal quality. Firstly, the peer review process of the International Journal of Clinical Case Reports and Reviews is rigorous, fair, transparent, fast, and of high quality. The editorial department invites experts from relevant fields as anonymous reviewers to review all submitted manuscripts. These experts have rich academic backgrounds and experience, and can accurately evaluate the academic quality, originality, and suitability of manuscripts. The editorial department is committed to ensuring the rigor of the peer review process, while also making every effort to ensure a fast review cycle to meet the needs of authors and the academic community. Secondly, the editorial team of the International Journal of Clinical Case Reports and Reviews is composed of a group of senior scholars and professionals with rich experience and professional knowledge in related fields. The editorial department is committed to assisting authors in improving their manuscripts, ensuring their academic accuracy, clarity, and completeness. Editors actively collaborate with authors, providing useful suggestions and feedback to promote the improvement and development of the manuscript. We believe that the support of the editorial department is one of the key factors in ensuring the quality of the journal. Finally, the International Journal of Clinical Case Reports and Reviews is renowned for its high- quality articles and strict academic standards. The editorial department is committed to publishing innovative and academically valuable research results to promote the development and progress of related fields. The International Journal of Clinical Case Reports and Reviews is reasonably priced and ensures excellent service and quality ratio, allowing authors to obtain high-level academic publishing opportunities in an affordable manner. I hereby solemnly declare that the International Journal of Clinical Case Reports and Reviews has a high level of credibility and superiority in terms of peer review process, editorial support, reasonable fees, and journal quality. Sincerely, Rui Tao.
Clinical Cardiology and Cardiovascular Interventions I testity the covering of the peer review process, support from the editorial office, and quality of the journal.
Clinical Cardiology and Cardiovascular Interventions, we deeply appreciate the interest shown in our work and its publication. It has been a true pleasure to collaborate with you. The peer review process, as well as the support provided by the editorial office, have been exceptional, and the quality of the journal is very high, which was a determining factor in our decision to publish with you.
The peer reviewers process is quick and effective, the supports from editorial office is excellent, the quality of journal is high. I would like to collabroate with Internatioanl journal of Clinical Case Reports and Reviews journal clinically in the future time.
Clinical Cardiology and Cardiovascular Interventions, I would like to express my sincerest gratitude for the trust placed in our team for the publication in your journal. It has been a true pleasure to collaborate with you on this project. I am pleased to inform you that both the peer review process and the attention from the editorial coordination have been excellent. Your team has worked with dedication and professionalism to ensure that your publication meets the highest standards of quality. We are confident that this collaboration will result in mutual success, and we are eager to see the fruits of this shared effort.
Dear Dr. Jessica Magne, Editorial Coordinator 0f Clinical Cardiology and Cardiovascular Interventions, I hope this message finds you well. I want to express my utmost gratitude for your excellent work and for the dedication and speed in the publication process of my article titled "Navigating Innovation: Qualitative Insights on Using Technology for Health Education in Acute Coronary Syndrome Patients." I am very satisfied with the peer review process, the support from the editorial office, and the quality of the journal. I hope we can maintain our scientific relationship in the long term.
Dear Monica Gissare, - Editorial Coordinator of Nutrition and Food Processing. ¨My testimony with you is truly professional, with a positive response regarding the follow-up of the article and its review, you took into account my qualities and the importance of the topic¨.
Dear Dr. Jessica Magne, Editorial Coordinator 0f Clinical Cardiology and Cardiovascular Interventions, The review process for the article “The Handling of Anti-aggregants and Anticoagulants in the Oncologic Heart Patient Submitted to Surgery” was extremely rigorous and detailed. From the initial submission to the final acceptance, the editorial team at the “Journal of Clinical Cardiology and Cardiovascular Interventions” demonstrated a high level of professionalism and dedication. The reviewers provided constructive and detailed feedback, which was essential for improving the quality of our work. Communication was always clear and efficient, ensuring that all our questions were promptly addressed. The quality of the “Journal of Clinical Cardiology and Cardiovascular Interventions” is undeniable. It is a peer-reviewed, open-access publication dedicated exclusively to disseminating high-quality research in the field of clinical cardiology and cardiovascular interventions. The journal's impact factor is currently under evaluation, and it is indexed in reputable databases, which further reinforces its credibility and relevance in the scientific field. I highly recommend this journal to researchers looking for a reputable platform to publish their studies.
Dear Editorial Coordinator of the Journal of Nutrition and Food Processing! "I would like to thank the Journal of Nutrition and Food Processing for including and publishing my article. The peer review process was very quick, movement and precise. The Editorial Board has done an extremely conscientious job with much help, valuable comments and advices. I find the journal very valuable from a professional point of view, thank you very much for allowing me to be part of it and I would like to participate in the future!”
Dealing with The Journal of Neurology and Neurological Surgery was very smooth and comprehensive. The office staff took time to address my needs and the response from editors and the office was prompt and fair. I certainly hope to publish with this journal again.Their professionalism is apparent and more than satisfactory. Susan Weiner
My Testimonial Covering as fellowing: Lin-Show Chin. The peer reviewers process is quick and effective, the supports from editorial office is excellent, the quality of journal is high. I would like to collabroate with Internatioanl journal of Clinical Case Reports and Reviews.
My experience publishing in Psychology and Mental Health Care was exceptional. The peer review process was rigorous and constructive, with reviewers providing valuable insights that helped enhance the quality of our work. The editorial team was highly supportive and responsive, making the submission process smooth and efficient. The journal's commitment to high standards and academic rigor makes it a respected platform for quality research. I am grateful for the opportunity to publish in such a reputable journal.
My experience publishing in International Journal of Clinical Case Reports and Reviews was exceptional. I Come forth to Provide a Testimonial Covering the Peer Review Process and the editorial office for the Professional and Impartial Evaluation of the Manuscript.
I would like to offer my testimony in the support. I have received through the peer review process and support the editorial office where they are to support young authors like me, encourage them to publish their work in your esteemed journals, and globalize and share knowledge globally. I really appreciate your journal, peer review, and editorial office.
Dear Agrippa Hilda- Editorial Coordinator of Journal of Neuroscience and Neurological Surgery, "The peer review process was very quick and of high quality, which can also be seen in the articles in the journal. The collaboration with the editorial office was very good."
I would like to express my sincere gratitude for the support and efficiency provided by the editorial office throughout the publication process of my article, “Delayed Vulvar Metastases from Rectal Carcinoma: A Case Report.” I greatly appreciate the assistance and guidance I received from your team, which made the entire process smooth and efficient. The peer review process was thorough and constructive, contributing to the overall quality of the final article. I am very grateful for the high level of professionalism and commitment shown by the editorial staff, and I look forward to maintaining a long-term collaboration with the International Journal of Clinical Case Reports and Reviews.
To Dear Erin Aust, I would like to express my heartfelt appreciation for the opportunity to have my work published in this esteemed journal. The entire publication process was smooth and well-organized, and I am extremely satisfied with the final result. The Editorial Team demonstrated the utmost professionalism, providing prompt and insightful feedback throughout the review process. Their clear communication and constructive suggestions were invaluable in enhancing my manuscript, and their meticulous attention to detail and dedication to quality are truly commendable. Additionally, the support from the Editorial Office was exceptional. From the initial submission to the final publication, I was guided through every step of the process with great care and professionalism. The team's responsiveness and assistance made the entire experience both easy and stress-free. I am also deeply impressed by the quality and reputation of the journal. It is an honor to have my research featured in such a respected publication, and I am confident that it will make a meaningful contribution to the field.
"I am grateful for the opportunity of contributing to [International Journal of Clinical Case Reports and Reviews] and for the rigorous review process that enhances the quality of research published in your esteemed journal. I sincerely appreciate the time and effort of your team who have dedicatedly helped me in improvising changes and modifying my manuscript. The insightful comments and constructive feedback provided have been invaluable in refining and strengthening my work".
I thank the ‘Journal of Clinical Research and Reports’ for accepting this article for publication. This is a rigorously peer reviewed journal which is on all major global scientific data bases. I note the review process was prompt, thorough and professionally critical. It gave us an insight into a number of important scientific/statistical issues. The review prompted us to review the relevant literature again and look at the limitations of the study. The peer reviewers were open, clear in the instructions and the editorial team was very prompt in their communication. This journal certainly publishes quality research articles. I would recommend the journal for any future publications.
Dear Jessica Magne, with gratitude for the joint work. Fast process of receiving and processing the submitted scientific materials in “Clinical Cardiology and Cardiovascular Interventions”. High level of competence of the editors with clear and correct recommendations and ideas for enriching the article.
We found the peer review process quick and positive in its input. The support from the editorial officer has been very agile, always with the intention of improving the article and taking into account our subsequent corrections.
My article, titled 'No Way Out of the Smartphone Epidemic Without Considering the Insights of Brain Research,' has been republished in the International Journal of Clinical Case Reports and Reviews. The review process was seamless and professional, with the editors being both friendly and supportive. I am deeply grateful for their efforts.
To Dear Erin Aust – Editorial Coordinator of Journal of General Medicine and Clinical Practice! I declare that I am absolutely satisfied with your work carried out with great competence in following the manuscript during the various stages from its receipt, during the revision process to the final acceptance for publication. Thank Prof. Elvira Farina
Dear Jessica, and the super professional team of the ‘Clinical Cardiology and Cardiovascular Interventions’ I am sincerely grateful to the coordinated work of the journal team for the no problem with the submission of my manuscript: “Cardiometabolic Disorders in A Pregnant Woman with Severe Preeclampsia on the Background of Morbid Obesity (Case Report).” The review process by 5 experts was fast, and the comments were professional, which made it more specific and academic, and the process of publication and presentation of the article was excellent. I recommend that my colleagues publish articles in this journal, and I am interested in further scientific cooperation. Sincerely and best wishes, Dr. Oleg Golyanovskiy.
Dear Ashley Rosa, Editorial Coordinator of the journal - Psychology and Mental Health Care. " The process of obtaining publication of my article in the Psychology and Mental Health Journal was positive in all areas. The peer review process resulted in a number of valuable comments, the editorial process was collaborative and timely, and the quality of this journal has been quickly noticed, resulting in alternative journals contacting me to publish with them." Warm regards, Susan Anne Smith, PhD. Australian Breastfeeding Association.
Dear Jessica Magne, Editorial Coordinator, Clinical Cardiology and Cardiovascular Interventions, Auctores Publishing LLC. I appreciate the journal (JCCI) editorial office support, the entire team leads were always ready to help, not only on technical front but also on thorough process. Also, I should thank dear reviewers’ attention to detail and creative approach to teach me and bring new insights by their comments. Surely, more discussions and introduction of other hemodynamic devices would provide better prevention and management of shock states. Your efforts and dedication in presenting educational materials in this journal are commendable. Best wishes from, Farahnaz Fallahian.
Dear Maria Emerson, Editorial Coordinator, International Journal of Clinical Case Reports and Reviews, Auctores Publishing LLC. I am delighted to have published our manuscript, "Acute Colonic Pseudo-Obstruction (ACPO): A rare but serious complication following caesarean section." I want to thank the editorial team, especially Maria Emerson, for their prompt review of the manuscript, quick responses to queries, and overall support. Yours sincerely Dr. Victor Olagundoye.
Dear Ashley Rosa, Editorial Coordinator, International Journal of Clinical Case Reports and Reviews. Many thanks for publishing this manuscript after I lost confidence the editors were most helpful, more than other journals Best wishes from, Susan Anne Smith, PhD. Australian Breastfeeding Association.
Dear Agrippa Hilda, Editorial Coordinator, Journal of Neuroscience and Neurological Surgery. The entire process including article submission, review, revision, and publication was extremely easy. The journal editor was prompt and helpful, and the reviewers contributed to the quality of the paper. Thank you so much! Eric Nussbaum, MD
Dr Hala Al Shaikh This is to acknowledge that the peer review process for the article ’ A Novel Gnrh1 Gene Mutation in Four Omani Male Siblings, Presentation and Management ’ sent to the International Journal of Clinical Case Reports and Reviews was quick and smooth. The editorial office was prompt with easy communication.
Dear Erin Aust, Editorial Coordinator, Journal of General Medicine and Clinical Practice. We are pleased to share our experience with the “Journal of General Medicine and Clinical Practice”, following the successful publication of our article. The peer review process was thorough and constructive, helping to improve the clarity and quality of the manuscript. We are especially thankful to Ms. Erin Aust, the Editorial Coordinator, for her prompt communication and continuous support throughout the process. Her professionalism ensured a smooth and efficient publication experience. The journal upholds high editorial standards, and we highly recommend it to fellow researchers seeking a credible platform for their work. Best wishes By, Dr. Rakhi Mishra.
Dear Jessica Magne, Editorial Coordinator, Clinical Cardiology and Cardiovascular Interventions, Auctores Publishing LLC. The peer review process of the journal of Clinical Cardiology and Cardiovascular Interventions was excellent and fast, as was the support of the editorial office and the quality of the journal. Kind regards Walter F. Riesen Prof. Dr. Dr. h.c. Walter F. Riesen.
Dear Ashley Rosa, Editorial Coordinator, International Journal of Clinical Case Reports and Reviews, Auctores Publishing LLC. Thank you for publishing our article, Exploring Clozapine's Efficacy in Managing Aggression: A Multiple Single-Case Study in Forensic Psychiatry in the international journal of clinical case reports and reviews. We found the peer review process very professional and efficient. The comments were constructive, and the whole process was efficient. On behalf of the co-authors, I would like to thank you for publishing this article. With regards, Dr. Jelle R. Lettinga.
Dear Clarissa Eric, Editorial Coordinator, Journal of Clinical Case Reports and Studies, I would like to express my deep admiration for the exceptional professionalism demonstrated by your journal. I am thoroughly impressed by the speed of the editorial process, the substantive and insightful reviews, and the meticulous preparation of the manuscript for publication. Additionally, I greatly appreciate the courteous and immediate responses from your editorial office to all my inquiries. Best Regards, Dariusz Ziora
Dear Chrystine Mejia, Editorial Coordinator, Journal of Neurodegeneration and Neurorehabilitation, Auctores Publishing LLC, We would like to thank the editorial team for the smooth and high-quality communication leading up to the publication of our article in the Journal of Neurodegeneration and Neurorehabilitation. The reviewers have extensive knowledge in the field, and their relevant questions helped to add value to our publication. Kind regards, Dr. Ravi Shrivastava.
Dear Clarissa Eric, Editorial Coordinator, Journal of Clinical Case Reports and Studies, Auctores Publishing LLC, USA Office: +1-(302)-520-2644. I would like to express my sincere appreciation for the efficient and professional handling of my case report by the ‘Journal of Clinical Case Reports and Studies’. The peer review process was not only fast but also highly constructive—the reviewers’ comments were clear, relevant, and greatly helped me improve the quality and clarity of my manuscript. I also received excellent support from the editorial office throughout the process. Communication was smooth and timely, and I felt well guided at every stage, from submission to publication. The overall quality and rigor of the journal are truly commendable. I am pleased to have published my work with Journal of Clinical Case Reports and Studies, and I look forward to future opportunities for collaboration. Sincerely, Aline Tollet, UCLouvain.
Dear Ms. Mayra Duenas, Editorial Coordinator, International Journal of Clinical Case Reports and Reviews. “The International Journal of Clinical Case Reports and Reviews represented the “ideal house” to share with the research community a first experience with the use of the Simeox device for speech rehabilitation. High scientific reputation and attractive website communication were first determinants for the selection of this Journal, and the following submission process exceeded expectations: fast but highly professional peer review, great support by the editorial office, elegant graphic layout. Exactly what a dynamic research team - also composed by allied professionals - needs!" From, Chiara Beccaluva, PT - Italy.
Dear Maria Emerson, Editorial Coordinator, we have deeply appreciated the professionalism demonstrated by the International Journal of Clinical Case Reports and Reviews. The reviewers have extensive knowledge of our field and have been very efficient and fast in supporting the process. I am really looking forward to further collaboration. Thanks. Best regards, Dr. Claudio Ligresti
Dear Chrystine Mejia, Editorial Coordinator, Journal of Neurodegeneration and Neurorehabilitation. “The peer review process was efficient and constructive, and the editorial office provided excellent communication and support throughout. The journal ensures scientific rigor and high editorial standards, while also offering a smooth and timely publication process. We sincerely appreciate the work of the editorial team in facilitating the dissemination of innovative approaches such as the Bonori Method.” Best regards, Dr. Matteo Bonori.
I recommend without hesitation submitting relevant papers on medical decision making to the International Journal of Clinical Case Reports and Reviews. I am very grateful to the editorial staff. Maria Emerson was a pleasure to communicate with. The time from submission to publication was an extremely short 3 weeks. The editorial staff submitted the paper to three reviewers. Two of the reviewers commented positively on the value of publishing the paper. The editorial staff quickly recognized the third reviewer’s comments as an unjust attempt to reject the paper. I revised the paper as recommended by the first two reviewers.
Dear Maria Emerson, Editorial Coordinator, Journal of Clinical Research and Reports. Thank you for publishing our case report: "Clinical Case of Effective Fetal Stem Cells Treatment in a Patient with Autism Spectrum Disorder" within the "Journal of Clinical Research and Reports" being submitted by the team of EmCell doctors from Kyiv, Ukraine. We much appreciate a professional and transparent peer-review process from Auctores. All research Doctors are so grateful to your Editorial Office and Auctores Publishing support! I amiably wish our article publication maintained a top quality of your International Scientific Journal. My best wishes for a prosperity of the Journal of Clinical Research and Reports. Hope our scientific relationship and cooperation will remain long lasting. Thank you very much indeed. Kind regards, Dr. Andriy Sinelnyk Cell Therapy Center EmCell
Dear Editorial Team, Clinical Cardiology and Cardiovascular Interventions. It was truly a rewarding experience to work with the journal “Clinical Cardiology and Cardiovascular Interventions”. The peer review process was insightful and encouraging, helping us refine our work to a higher standard. The editorial office offered exceptional support with prompt and thoughtful communication. I highly value the journal’s role in promoting scientific advancement and am honored to be part of it. Best regards, Meng-Jou Lee, MD, Department of Anesthesiology, National Taiwan University Hospital.
Dear Editorial Team, Journal-Clinical Cardiology and Cardiovascular Interventions, “Publishing my article with Clinical Cardiology and Cardiovascular Interventions has been a highly positive experience. The peer-review process was rigorous yet supportive, offering valuable feedback that strengthened my work. The editorial team demonstrated exceptional professionalism, prompt communication, and a genuine commitment to maintaining the highest scientific standards. I am very pleased with the publication quality and proud to be associated with such a reputable journal.” Warm regards, Dr. Mahmoud Kamal Moustafa Ahmed
Dear Maria Emerson, Editorial Coordinator of ‘International Journal of Clinical Case Reports and Reviews’, I appreciate the opportunity to publish my article with your journal. The editorial office provided clear communication during the submission and review process, and I found the overall experience professional and constructive. Best regards, Elena Salvatore.
Dear Mayra Duenas, Editorial Coordinator of ‘International Journal of Clinical Case Reports and Reviews Herewith I confirm an optimal peer review process and a great support of the editorial office of the present journal
Dear Editorial Team, Clinical Cardiology and Cardiovascular Interventions. I am really grateful for the peers review; their feedback gave me the opportunity to reflect on the message and impact of my work and to ameliorate the article. The editors did a great job in addition by encouraging me to continue with the process of publishing.
Dear Cecilia Lilly, Editorial Coordinator, Endocrinology and Disorders, Thank you so much for your quick response regarding reviewing and all process till publishing our manuscript entitled: Prevalence of Pre-Diabetes and its Associated Risk Factors Among Nile College Students, Sudan. Best regards, Dr Mamoun Magzoub.
International Journal of Clinical Case Reports and Reviews is a high quality journal that has a clear and concise submission process. The peer review process was comprehensive and constructive. Support from the editorial office was excellent, since the administrative staff were responsive. The journal provides a fast and timely publication timeline.
Dear Maria Emerson, Editorial Coordinator of International Journal of Clinical Case Reports and Reviews, What distinguishes International Journal of Clinical Case Report and Review is not only the scientific rigor of its publications, but the intellectual climate in which research is evaluated. The submission process is refreshingly free of unnecessary formal barriers and bureaucratic rituals that often complicate academic publishing without adding real value. The peer-review system is demanding yet constructive, guided by genuine scientific dialogue rather than hierarchical or authoritarian attitudes. Reviewers act as collaborators in improving the manuscript, not as gatekeepers imposing arbitrary standards. This journal offers a rare balance: high methodological standards combined with a respectful, transparent, and supportive editorial approach. In an era where publishing can feel more burdensome than research itself, this platform restores the original purpose of peer review — to refine ideas, not to obstruct them Prof. Perlat Kapisyzi, FCCP PULMONOLOGIST AND THORACIC IMAGING.
Dear Mayra Duenas, Editorial Coordinator of the journal IJCCR, I write here a little on my experience as an author submitting to the International Journal of Clinical Case Reports and Reviews (IJCCR). This was my first submission to IJCCR and my manuscript was inherently an outsider’s effort. It attempted to broadly identify and then make some sense of life’s under-appreciated mysteries. I initially had responded to a request for possible submissions. I then contacted IJCCR with a tentative topic for a manuscript. They quickly got back with an approval for the submission, but with a particular requirement that it be medically relevant. I then put together a manuscript and submitted it. After the usual back-and-forth over forms and formality, the manuscript was sent off for reviews. Within 2 weeks I got back 4 reviews which were both helpful and also surprising. Surprising in that the topic was somewhat foreign to medical literature. My subsequent updates in response to the reviewer comments went smoothly and in short order I had a series of proofs to evaluate. All in all, the whole publication process seemed outstanding. It was both helpful in terms of the paper’s content and also in terms of its efficient and friendly communications. Thank you all very much. Sincerely, Ted Christopher, Rochester, NY.
Dear Grace Pierce, Editorial Coordinator of the journal IJCCR, I had a very positive experience with Auctores - Journal throughout the publication process. The Editorial Team was highly responsive, professional, and supportive at every stage. I would like to extend my sincere thanks to the Editor: Grace Pierce, for her guidance and assistance. The peer-review process was smooth and constructive, helping improve the quality of my work. I would gladly recommend Auctores Journal to fellow researchers and authors. Dr. SABITA SINHA, Medical Oncologist, MD (Electro Homeopathy).
